LOCUS       RR213845                8433 bp    DNA     circular  SYN 
DEFINITION  Cilp (Myc-DDK-tagged ORF) - Rat cartilage intermediate layer protein, nucleotide pyrophosphohydrolase (Cilp), (10 ug)
ACCESSION   NM_001108161
VERSION     NM_001108161.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4590..4681 
                     /label="Myc-DDK"  
     misc_feature    4711..5296 
                     /label="PolyA signal"  
     misc_feature    4768..4787 
                     /label="Restriction Enzyme"  
     misc_feature    5572..6244 
                     /label="ColE1"  
     misc_feature    6674..7468 
                     /label="Kan/Neo"  
     misc_feature    7503..7805 
                     /label="SV40 ori"  
     misc_feature    7891..8368 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4572..4578 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4572 
                     /label="Cilp"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCAGCAACC AAGACCTGGG TACTCTCTTT CCTGGTCCTT GAAGTCACCA 
     1081 CCGTGCTGGG GAGACAGATG ATGCTTACCC AGTCAGCAAG AAGAGTCCAG CCTGTGAAGA 
     1141 GGACCCCTAA GATCCTCACC AAGCCTGTTA ACTCCCAGGA GAGTCCTGGA GAGTGGACGA 
     1201 CTTGGTTTAA CATCGACCAC CCAGGTGGGC AGGGTGACTA TGAGCGACTG GATGCCATTC 
     1261 GATTCTACTA CGGGGAGCGC GTGTGCGCCC GTCCCCTCCG GCTAGAGGCT CGGACCACTG 
     1321 ACTGGATGCC TGCGGGCATC ACTGGCCAGG TGGTCCATGG CAGTCCCCGT GAGGGCTTCT 
     1381 GGTGCCTCAA CAAGGAACAG AGACCAGGCC AGAACTGTTC CAATTACACA GTGCGCTTCC 
     1441 TCTGCCCACC AGGGTCCTTG CGGAGAGATA CAGAGCATAT CTGGAGTTCC TGGTCTCCCT 
     1501 GGAGCGCGTG CTCAGCCGCC TGTGGTCACA CCGGGGTCCA GACCCGTACC CGCACCTGCT 
     1561 TGGCACAGAC AGTGTCACTA TGTAATGAGG CCACTGAGGA GGGACAGCTC TGCATGAGCC 
     1621 AGGCCTGCAC AGCTTGTGAC CTGACCTGCC CCATGGGCCA GGTAAATGCT GACTGTGATG 
     1681 CCTGCATGTG TCAGGACTTC ATGCTTCACG GGGCCATCTC CCTCCCTGGA GGTGGCCCAG 
     1741 CTCCAGGGGC CACTGTCTAC CTGCTGGCTA AGGCACCAAA GATGCTGACC CAAACAGACA 
     1801 GCAGCGGAAG TTTCCGAGTT CCTGGCTTGT GTCCTGATGG CAAAACCGTC CTGAAAGTTA 
     1861 CCAAGAGCAA GTTTGCCCCA ATTATGATCA CGATGCCCAA GACTAGCCTG AAGTCAGCCA 
     1921 CCATCAACGC AGAGTTTGTG AGGGCAGAGA CCCCATACAT TGTAATGAAC CCTGAGACGA 
     1981 AAGCACGTCG GGCTGGGCAG AGTGTGTCTC TGTGCTGCAA GGCCACAGGG AAACCTAGTC 
     2041 CAGACAAGTA CTTCTGGTAC CACAACAACA CATTGCTGGA TCCTTCCCTC TATAAACACC 
     2101 AGAGCAAGCT GGTGCTGAGG AACCTGCAGC AGGACCAAGC AGGGGAGTAC TTCTGTAAGG 
     2161 CGCAGAGTGA TGCTGGGGCT GTGAAGTCCA AGGTCACCCA ACTCACTGTC ATTGCACACG 
     2221 ATGAGGCTCC TTGCAACCCA ACCCCAGAGA GCTACCTTAT CCGGCTGCCC CATGATTGCT 
     2281 TCCAGAATGC CACCAACTCC TTCTACTATG ATGTAGGCCG CTGCCCCATC AAGACCTGTG 
     2341 CAGGGCAGCA AGACAATGGG ATCCGGTGCC AAGATGCCGT GGAGAACTGC TGTGGTATTT 
     2401 CCAGAACAGA GGAGAGGGAG ATCCAGTGCA GTGGGTACAC ACTGCCCACC AAGGTGGCCA 
     2461 TGGAGTGCAG CTGTCAACGA TGTGCAGAGA CACGGAGCAT CGTTCGGGGC CGGGTCACTG 
     2521 CTGCTGACGA TGGGGAGCCC ATGCGCTTTG GCCACGTGTA CATGGGGAAT AACCGTGTGA 
     2581 GCATGACTGG CTACAAAGGC ACATTCACCC TCCACGTCCC CCAGGACACG GAGAGGCTGG 
     2641 TGCTCACGTT TGTGGACAGG CTGCAGAAAT TTGTCAACAC TACCAAAGTG CTGCCCTTCA 
     2701 ACAAGAAAGG GAGCGCAGTG TTCCATGAGA TCAAGATGCT TCGGCAGAAA GAGCCCATCA 
     2761 CGTTGGAAGC CACGGAAACC AACATTATCC CCCTGGGAGA GGTGGCTGGT GAGGATCCTG 
     2821 TGGCTGAACT GGAGATTCCA CCCAAAAGTT TCTATAAGCA GAATGGGGAG CCCTTCACAG 
     2881 GAAAAGTAAA GGCCAGTGTG ACCTTCCTGG ATCCTCGGAA TGTCTCTACG GCCACAGCCA 
     2941 CCCAAAGTGA CCTGAACTTC ATTAATGATG AAGGAGACAC CTTTCCCCTT AGAACATACG 
     3001 GGATGTTTTC TGTGGACTTC AGAGATGAGG CCACTTCGGA GTCACTTAAT GCTGGCAAGG 
     3061 TGAAGGTCCA TCTTGACTCG ACCCAGGTCA AAATGCCAGA GCATGCGCGG GCCATGAAAC 
     3121 TCTGGTCGCT CAACCCAAAC ACGGGGCTGT GGGAAGAGGA AGGCGATTTC AAATTTGAAA 
     3181 GCCAGAGGAG GAACAAGAGG GAAGAGAGAA CCTTCCTAGT GGGAAATATG GAGATCCGTG 
     3241 AGAGAAGGCT CTTTAACCTG GATGTCCCAG AAAGCAGAAG GTGCTTCATC AAGGTGAGGA 
     3301 CTTATCGGAG TGAGAGGTTC CTGCCCAGTG AGCAAATCCA GGGGGTTGTA GTCTCAGTGA 
     3361 TCAACCTGGA GCCCAGAACT GGCTTTTCAT CTAACCCCAG GGCCTGGGGC CGATTTGACA 
     3421 GTGTCATCAC AGGGCCCAAT GGAGCCTGCC TGCCTGCCTT CTGTGACGAT CAGTCCCCTG 
     3481 ATGCCTACTC TGTCTATGTT TTGGCAAGCC TTTCTGGAGA AGAACTAGAG GCAGTTGAGT 
     3541 CTTCTCCTAA ATTCAACCCA AATGCAATCG GTGTCCCTCA GCCTTACCTC AACAAACTTA 
     3601 AATACCGTCG GACAGACCAT GAGGATCCAC GGGTTAAAAA GACGGCTTTC CAAATCAGTA 
     3661 TGGCCAAACC AAGGCCCAAC TCAGCTGAGG AGAGCAATGG GCCCATCTAT GCCTTTGAGA 
     3721 ACCTTCGGGC ATGCGAGGAG GCACCACCCA GTGCAGCTCA CTTCCGGTTC TATCAGATTG 
     3781 AGGGGGATCG CTATGACTAT AACACAGTTC CTTTCAATGA AGATGACCCT ATGAGCTGGA 
     3841 CTGAAGACTA CCTGGCATGG TGGCCCAAGC CAATGGAGTT CAGAGCCTGC TACATCAAGG 
     3901 TAAAGATTGT GGGGCCACTT GAGGTGAATG TACGATCCCG TAACATGGGC GGCACCCACC 
     3961 GGCAGACTGT GGGGAAGCTC TATGGGATCC GGGATGTGAA AAGTACTCGG GACAGAGACC 
     4021 AACCTAATGT CTCATCTGCC TGTCTGGAGT TCAAGTGCAG TGGAATGCTG TATGACCAAG 
     4081 ACCGTGTAGA CCGCACATTA GTGAAGGTCA TCCCCCAGGG CAGCTGCCAT CGAGCCAGTG 
     4141 TTAACTCTAT GCTGCACGAG TACCTGGTCA ACCACCTTCC CCTGGCAGTC AACAATGACA 
     4201 CCAGCGAGTA TACCATGCTG GCACCCCTGG ACCCTCTGGG CCACAATTAC GGTATCTATA 
     4261 CTGTCACTGA CCAGGACCCT GGTACAGCTA AGGAGATTGC GCTTGGCCGG TGCTTTGACG 
     4321 GCACTTCTGA TGGCTCCTCC AGAATCATGA AGAGCAACGT GGGAGTCGCC CTGACCTTTA 
     4381 ACTGTGCAGA AAGGCAGGTA GGCCGCCAGA GCGCCTTCCA GTACCTCCAA AGCACCCCGG 
     4441 CCCGGTCCCC AGCTACAGGC ACTGTCCAAG GAAGAGTACC CGCCATGAGG CAACAACGGG 
     4501 CAAGCAGGGG TGGCCTACGC CGGCGTGGAA GCATGGCCCC TCTGAGATTT TCTGGTGTCG 
     4561 CTCAACAACC TCTGAGCAAC ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4621 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4681 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4741 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4801 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4861 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4921 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4981 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5041 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5101 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5161 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5221 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5281 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5341 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5401 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5461 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5521 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5581 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5641 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5701 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5761 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5821 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5881 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5941 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6001 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6061 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6121 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6181 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6241 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6301 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6361 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6421 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6481 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6541 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6601 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6661 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6721 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6781 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6841 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6901 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6961 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7021 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7081 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7141 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7201 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7261 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7321 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7381 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7441 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7501 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7561 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7621 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7681 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7741 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7801 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7861 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7921 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7981 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8041 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8101 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8161 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8221 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8281 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8341 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8401 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//