LOCUS       RR213810                7548 bp    DNA     circular  SYN 
DEFINITION  Zfp281 (Myc-DDK-tagged ORF) - Rat zinc finger protein 281 (Zfp281), (10 ug)
ACCESSION   NM_001012030
VERSION     NM_001012030.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3705..3796 
                     /label="Myc-DDK"  
     misc_feature    3826..4411 
                     /label="PolyA signal"  
     misc_feature    3883..3902 
                     /label="Restriction Enzyme"  
     misc_feature    4687..5359 
                     /label="ColE1"  
     misc_feature    5789..6583 
                     /label="Kan/Neo"  
     misc_feature    6618..6920 
                     /label="SV40 ori"  
     misc_feature    7006..7483 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3687..3693 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3687 
                     /label="Zfp281"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAATCGGC AGCGGGTTCC TCAGCGGCGG CGGCGGTCCC GGCAGTAGCG 
     1081 GTGGTAGCGG CTCCAGCGGC AGCTCCGGCA GCGGCGGCAG CAGGAGAGCT GAGATGGAGC 
     1141 CCACCTTCCC CCAGAGTATG GTTATGTTCA ACCACCGGCT CCCCCCGGTC ACCAGCTTCA 
     1201 CCCGGCCTGC GGGGACGGCC GCCCCTCCCC CGCAGTGCGT GTTATCCTCC TCTACCTCCG 
     1261 CAGCCCCGGC CGCTGAGCCC CCCCCTCCAC CAGCCCCGGA CATGACTTTC AAGAAGGAGC 
     1321 CGGCGGCGTC AGCCGCGGCC TTCCCTTCGC AGAGGACCTC CTGGGGATTC TTGCAGTCCT 
     1381 TGGTGAGCAT CAAGCAGGAG AAACCTGCGG ATCCTGAGGA GCAGCAGTCC CACCACCATC 
     1441 ATCACCACCA CCACTATGGG GGGCTGTTCG CTGGGGCTGA AGAGAGATCA CCAGGCCTAG 
     1501 GAGGAGGGGA AGGGGGGAGC CACGGCGTCA TCCAAGACCT CAGTATTCTC CACCAGCATG 
     1561 TCCAGCAGCA ACCAGCCCAG CATCACCGCG ATGTATTACT GAGCAGCAGC GGTAGCAGGA 
     1621 CTGATGACCA TGGCAACGAG GAGCCAAAGC AGGACACTAA CGTCAAAAAG GCAAAGAGGC 
     1681 CAAAGCCAGA ATCTCAGGGA ATCAAAGCCA AGAGGAAGCC AAGTGCATCT TCCAAACCTT 
     1741 TGGTTGGAGA GGGAGAAGGT GCCGTCCTGT CCCCAAGTCA GAAACCTCAT ATCTGTGATC 
     1801 ACTGTAGTGC TGCTTTCAGG AGCTCCTACC ACCTGCGGAG ACATGTCCTC ATTCACACAG 
     1861 GAGAAAGGCC GTTCCAGTGC AGCCAGTGTA GTATGGGTTT CATTCAGAAA TACCTACTGC 
     1921 AGAGACACGA GAAAATTCAC AGCAGAGAGA AGCCCTTTGG GTGCGATCAG TGCAGCATGA 
     1981 AGTTTATTCA GAAGTACCAT ATGGAGAGAC ACAAGAGGAC ACATAGTGGA GAAAAGCCAT 
     2041 ATAAGTGTGA CACTTGCCAA CAGTATTTTT CAAGGACTGA CAGATTGTTG AAGCACAGGC 
     2101 GCACGTGTGG TGAAGCCATA GCAAAAGGGG CCGCTAGTGC AGAACCTGGG TCATCAAACC 
     2161 ATAATAATAT GGGTAATCTG GCTGTGTTGT CTCAGGGAAA TACAAGTTCT TCACGGAGAA 
     2221 AATCGAAGTC AAAAAGCATA GCTGTTGAAA ATAAGGAACA CAAGACTGGC AAAACAAACG 
     2281 AATCACAGAT GTCAAACAAT ATAAACATGC AGAGTTACTC AGTAGAAATG CCTACTGTGT 
     2341 CCACCAGTGG CGGCATCATC GGCACGGGGC TCGATGAGCT ACAGAAAAGG GTGCCAAAAC 
     2401 TGATCTTTAA GAAAGGAAGC AGAAAGAATA CAGATAAAAG CTACCTTAAT TTTGTGTCAC 
     2461 CGTTACCAGA TGTAGCTGGG CAGAAATCCT TGTCTGGTAA ACCAAGTGGC TCGCTTGGCA 
     2521 TCGTATCGAA CAATAGCGTG GAGACCATTA GTCTTCTCCA AAGTGCAAGC GGCAAACCAG 
     2581 GCCCGATAAG TAGTAATTAT GATGATGCCA TGCAGTTCTC AAAGAAGAGA AGATACTTAC 
     2641 CAACTGCCAG CAGCAACAGT GCCTTTTCTA TCAATGTAGG ACACATGGTC TCCCAGCAGT 
     2701 CAGTCATTCA GTCTGCAGGG GTCAGTGTTT TGGACAATGA GGCGCCATTG TCACTTATTG 
     2761 ACTCCTCAGC TCTAAATGCT GAAATTAAGT CTTGTCACGA CAAGTCTGGA ATTCCTGATG 
     2821 AGGTTCTACA AAGTATTTTG GATCAGTACT CTAGCAAGTC AGAACCACAG AAGGAGGATC 
     2881 CCTTCAACTT AACAGAACCA CGAGTGGATT TACACACCTC AGGAGAACAC TCTGAATTGG 
     2941 TTCAAGAAGA GCATTTGAGC CCAGGCACCC AGACACCTTC AAATGACAAA GCAAGCGTGT 
     3001 TGCAGGAGTA CTCCAAATAC CTCCAGCAGG CTTTTGAGAA GTCCACGAAT GCAGGTTTTA 
     3061 CCCTCGGACA TGGTTTCCAG TTTGTCAGCT TATCTTCACC TCTCCACAAC CACACTTTGT 
     3121 TTCCAGAAAA ACAGATATAC ACTACATCTC CTTTGGAGTG TGGTTTTGGC CAATCTGTTA 
     3181 CCTCAGTGTT GCCATCTTCA TTGCCAAAGC CTCCTTTTGG GATGTTGTTT GGATCTCAAC 
     3241 CAGGTCTTTA TTTATCTGCT TTGGATGCTA CACATCAGCA GTTGACACCT TCCCAGGAGC 
     3301 TGGATGATCT GATAGATTCT CAGAAGAACT TAGAGACATC ATCAGCCTTC CAGTCCTCAT 
     3361 CTCAGAAATT GACTAGCCAG AAGGAACAAC AGAAAAATTT AGAGTCCTCA ACAAGCTTTC 
     3421 AGATTCCATC TCAGGAGTTA GCTAGCCAGA TTGATCCTCA GAAAGACATA GAGCCTAGAA 
     3481 CAACGTACCA GATTGAGAAC TTTGCACAAG CATTCGGTTC TCAGTTTAAG TCGGGCAGCA 
     3541 GGGTGCCAAT GACCTTTATC ACTAACTCTA ATGGAGAAGT GGACCATAGA GTAAGGACTT 
     3601 CAGTGTCAGA TTTCTCAGGG TATACAAATA TGATGTCTGA TGTAAGTGAG CCATGTAGTA 
     3661 CAAGAGTAAA GACACCCACC AGCCAGAGTT ACAGGACGCG TACGCGGCCG CTCGAGCAGA 
     3721 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3781 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3841 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     3901 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     3961 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4021 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4081 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4141 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4201 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4261 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4321 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4381 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4441 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4501 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4561 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4621 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4681 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4741 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4801 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4861 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     4921 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     4981 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5041 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5101 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5161 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5221 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5281 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5341 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5401 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5461 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5521 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5581 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5641 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5701 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5761 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5821 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     5881 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     5941 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6001 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6061 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6121 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6181 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6241 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6301 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6361 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6421 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6481 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6541 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6601 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6661 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6721 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6781 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6841 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     6901 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     6961 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7021 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7081 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7141 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7201 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7261 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7321 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7381 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7441 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7501 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//