LOCUS       RR213792                7998 bp    DNA     circular  SYN 
DEFINITION  Pld1 (Myc-DDK-tagged ORF) - Rat phospholipase D1 (Pld1), (10 ug)
ACCESSION   NM_030992
VERSION     NM_030992.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4155..4246 
                     /label="Myc-DDK"  
     misc_feature    4276..4861 
                     /label="PolyA signal"  
     misc_feature    4333..4352 
                     /label="Restriction Enzyme"  
     misc_feature    5137..5809 
                     /label="ColE1"  
     misc_feature    6239..7033 
                     /label="Kan/Neo"  
     misc_feature    7068..7370 
                     /label="SV40 ori"  
     misc_feature    7456..7933 
                     /label="f1 ori"  
     misc_feature    1029..1037 
                     /label="Restriction Enzyme"  
     misc_feature    4137..4143 
                     /label="Restriction Enzyme"  
     misc_feature    1029..4137 
                     /label="Pld1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCGG CGCGCCCATG TCACTAAGAA GTGAGGCCCG GGTGAACACT TCTACACTGC 
     1081 AGAAGATCGC CGCAGATATG AGAAACCTGA TAGAAAATCT CGACACCCGG GAACTTCATT 
     1141 TTGAGGGAGA GGAGGTGGAG TATGATGCAT CTCCAGGAGA CCCCACGGCT CAGGAAGCGT 
     1201 GCATCCCATT CTCTTCTATT TACAACACTC AAGGATTTAA GGAGCCCAAC ATCCAGATCT 
     1261 ACCTCTCTGG CTGTCCTGTA AAAGCACAAG TACTGGAAGT GGAGCGCTTC ACGTCGACCT 
     1321 CGAGGATGCC AAGTGTCAAC CTGTACACCA TCGAGTTAAC ACACGGGGAA TTCACATGGC 
     1381 AAGTTAAGAG GAAATTCAAG CACTTCCAAG AGTTTCATAG AGAGCTCCTC AAGTACAAAG 
     1441 CCTTCATCCG GATCCCCATT CCCACCAAAA GACATACGTT TAGAAGACAA AATGTCAAAG 
     1501 AAGAGCCCAG AGAAATGCCC AGTCTGCCCC GTTCATCAGA AAATGCAATC CAGGAGGAGC 
     1561 AGTTCTTTGG CAGGAGGAAG CAACTGGAAG ATTACTTGAC AAAAATACTA AAAATGCCCA 
     1621 TGTACAGAAA CTATCATGCC ACGACAGAGT TCCTTGATGT AAGCCAACTG TCTTTTATCC 
     1681 ATGATTTGGG ACCAAAGGGC CTGGAAGGAA TGATCATGAA AAGATCTGGG GGACACAGGA 
     1741 TACCAGGTGT GAATTGCTGT GGCCATGGAA GAGCCTGCTA CCGGTGGTCA AAGAGGTGGT 
     1801 TGATAGTGAA AGACTCTTTC CTGCTGTATA TGAAACCAGA CAGCGGGGCG ATCGCCTTCG 
     1861 TCCTTCTTGT AGACAAAGAA TTCAGAATCA AAGTGGGAAA GAAGGAGACA GAAACGAAAT 
     1921 ATGGACTTCG AATTGATAAT CTTTCAAGGA CACTGATTTT AAAATGTAAC AGCTACAGAC 
     1981 ATGCCCGGTG GTGGGGAGGC GCCATCGAGG AGTTCATCCA GAAGCACGGC ACTGACTTCC 
     2041 TCAAAGACCA TCGCTTCGGC TCCTACGCTG CGGTCCACGA GAACATTTTA GCTAAATGGT 
     2101 ACGTGAACGC CAAAGGTTAC TTTGAAGATA TCGCAAACGC AATGGAAGGG GCAACGGAAG 
     2161 AGATTTTTAT CACAGATTGG TGGTTAAGTC CAGAAATCTT CCTGAAGCGC CCAGTGGTGG 
     2221 AAGGCAACCG CTGGAGGCTG GACTGCATCC TCAAACGGAA AGCACAACAA GGTGTGCGGA 
     2281 TTTTCATAAT GCTTTACAAA GAGGTGGAGC TAGCCCTCGG GATCAACAGC GAGTACACCA 
     2341 AGCGGACTTT GATGCGGCTG CACCCCAACA TAAAGGTGAT GAGACACCCA GACCATGTGT 
     2401 CCTCCAGTGT GTATCTGTGG GCTCATCACG AGAAGCTCGT CATTATCGAC CAATCGGTGG 
     2461 CGTTTGTGGG TGGGATTGAC CTGGCCTATG GACGGTGGGA TGACAATGAG CACAGACTCA 
     2521 CAGATGTGGG CAGTGTCAAG CGGGTCACCT CAGGACAGTC TCTGGGCTCT CTCACAGCAG 
     2581 CATCAGTAGA GTCTATGGAA TCCTTAAGCC TCAAGGACAA ACATCAATCT CATAAAAACG 
     2641 AGCCCGTCTT AAAGAGTGTC AACGATACCG ACATGAAACT GAAAGGCATA GGGAAGTCCA 
     2701 GGAAATTCTC CAAATTTAGC CTCTATCGCC AACTTCACCG CCGTAATCTG CACAACTCGG 
     2761 ACAGCATTAG CAGCGTCGAC AGCGCCTCCA ACACCGGCTC CATCCGAAGT GTGCAGACAG 
     2821 GAGTGGGAGA GCTCCATGGG GAGACTAGGT TTTGGCATGG GAAGGATTAC TGCAACTTTG 
     2881 TCTTCAAGGA CTGGGTTCAA CTGGACAAAC CTTTTGCTGA TTTCATCGAC AGGTACTCCA 
     2941 CCCCCCGGAT GCCTTGGCAT GACATTGGTT CTGTGGTTCA CGGAAAGGCA GCTCGTGACG 
     3001 TGGCTCGACA CTTCATCCAG CGCTGGAACT TCACAAAGAT TATGAAGCCA AAATATCGTT 
     3061 CCCTTTCTTA TCCTTTCCTG CTCCCAAAGT CTCAAGCCAC AGCGCATGAG CTGAGGTATC 
     3121 AGGTGCCAGG GGCCGTCCAC GCCAAAGCGC AGTTGCTCCG ATCTGCTGCT GACTGGTCTG 
     3181 CTGGTATAAA GCANCACGAG GAATCTATCC ATGCTGCCTA CACCCATGTC ATAGAGAACA 
     3241 GCAAGCACTA CATCTATATT GAAAACCAAT TTTTCATAAG CTGCGCTGAT GATAAAGTCG 
     3301 TGTTCAACAA GGTGGGCAAT GCTATCGCCC AGAGAATCCT GAAAGCCCAC AGGGAAGGCC 
     3361 AGAGATATCG AGTGTACATC GTGATACCAC TGCTGCCGGG ATTCGAAGGA GACATTTCCA 
     3421 CAGGCGGGGG AAATGCCCTA CAGGCAATAA TGCACTTCAA CTACAGAACC ATGTGCAGAG 
     3481 GGGAGAGCTC CATCCTTGAA CAGTTAAAGC CAGAGCTTGG TAACAAGTGG ATAAATTACA 
     3541 TATCATTTTG CGGTCTGAGA ACACATGCAG AGCTGGAAGG AAACCTTGTC ACTGAGCTCA 
     3601 TCTATGTCCA CAGCAAGTTG TTAATTGCTG ATGATAACAC GGTTATCATA GGTTCTGCCA 
     3661 ACATCAATGA CCGGAGCATG CTGGGAAAGC GAGACAGCGA AATGGCTGTC ATAGTGCAGG 
     3721 ACAGACAGAC AGTCCCGTCA GTGATGGATG GAAAAGAATA TCAAGCTGGC CGCTTCGCTC 
     3781 AAGGCCTTCG GCTGGAGTGC TTCAGGCTTG TCCTGGGTTA TCTTTCTGAC CCAAGTGAGG 
     3841 ACATTCAGGA TCCTGTAAGT GACAAATTCT TCAAGGAGAT TTGGGTTTCA ACGGCAGCTC 
     3901 GAAATGCTAC CATATATGAT AAGGTCTTCC GGTGCCTTCC CAATGACGAA GTGCACAATT 
     3961 TAATCCAGTT GCGGGACTTT ATAAACAAGC CTATATTGGC AAAAGAGGAC CGCCTTCGTG 
     4021 CCGAGGAAGA ACTGAGGAAG ATCCGTGGCT TCTTGGTGCA GTTCCCCTTC TACTTCTTAT 
     4081 CAGAAGAAAA CCTGCTGCCT TCCGTTGGAA CCAAGGAAGC CATTGTGCCC ATGGAGGTTT 
     4141 GGACTACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     4201 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     4261 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4321 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4381 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4441 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4501 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4561 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4621 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4681 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4741 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4801 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4861 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4921 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4981 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     5041 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     5101 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     5161 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     5221 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     5281 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5341 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5401 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5461 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5521 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5581 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5641 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5701 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5761 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5821 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5881 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5941 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     6001 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     6061 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     6121 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     6181 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     6241 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6301 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6361 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6421 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6481 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6541 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6601 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6661 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6721 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6781 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6841 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6901 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6961 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     7021 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     7081 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     7141 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     7201 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     7261 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7321 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7381 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7441 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7501 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7561 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7621 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7681 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7741 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7801 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7861 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7921 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7981 AAATTTAACG CGAATTTT
//