LOCUS       RR213790                9291 bp    DNA     circular  SYN 
DEFINITION  Magi3 (Myc-DDK-tagged ORF) - Rat membrane associated guanylate kinase, WW and PDZ domain containing 3 (Magi3), (10 ug)
ACCESSION   NM_139084
VERSION     NM_139084.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5448..5539 
                     /label="Myc-DDK"  
     misc_feature    5569..6154 
                     /label="PolyA signal"  
     misc_feature    5626..5645 
                     /label="Restriction Enzyme"  
     misc_feature    6430..7102 
                     /label="ColE1"  
     misc_feature    7532..8326 
                     /label="Kan/Neo"  
     misc_feature    8361..8663 
                     /label="SV40 ori"  
     misc_feature    8749..9226 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5430..5436 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5430 
                     /label="Magi3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCGAAGACG TTGAAGAAGA AGAAGCATTG GCTCAGCAAG GTGCAGGAGT 
     1081 GTGCGGTGTC CTGGGCCGGG CCCCCGGGCG ACTTGGGCGC TGAGATCCGC GGCGGCGCGG 
     1141 AGCGCGGCGA GTTCCCTTAC CTGGGGCGGC TCCGTGACGA GCCTGGCGGC GGCGGAGGCA 
     1201 CCTGCTGCGT GGTCTCGGGC AAGGCGCCCA GTCCTGGCGA TGTGCTGTTG GAGGTGAACG 
     1261 GGACGCCCGT CAGTGGGCTC ACCAACCGGG ACACCCTGGC TGTCATCCGC CACTTCCGCG 
     1321 AGCCAATCCG TCTGAAGACC GTGAAGCCAG GCAAAGTCAT AAATAAAGAT TTGCGACATT 
     1381 ACCTGAGTCT GCAGTTTCAG AAAGGATCGA TTGACCACAA ACTACAGCAG GTCATCAGAG 
     1441 ATAACCTCTA CTTGATAACC ATCCCATGCA CTACAAGGGC TCCCAGGGAT GGGGAAGTTC 
     1501 CAGGGGTGGA CTATAATTTC ATTTCTGTTG AACAATTCAA GGCACTGGAA GAAAGCGGAG 
     1561 CATTGTTAGA AAGTGGAACG TATGATGGAA ATTTCTATGG CACGCCCAAG CCTCCAGCAG 
     1621 AGCCTAGTCC TTTCCAGCCC GATCCAGTTG ATCAGGTCCT CTTTGACAAT GAGTTTGACA 
     1681 CAGAATCCCA AAGAAAACGA ACTACATCTG TCAGCAAGAT GGAAAGGATG GACAGCTCTC 
     1741 TCCCTGAGGA AGAGGAAGAT GAGGACAAAG AAGCTGTCAA TGGCAGCGGA AGCATGGAAA 
     1801 CTAGAGAGAT GCATTCTGAG TCGTCTGACT GCTGGATGAA GACTGTTCCA AGTTATAACC 
     1861 AAACAAATCG CTCCATGGAC TTTAGAAATT ATATGATGAG AGACGAGAAT CTGGAGCCGC 
     1921 TGCCCAAAAA CTGGGAAATG GCCTACACTG ACACAGGAAC GATCTACTTC ATTGATCACA 
     1981 ATACGAAGAC AACCACCTGG CTGGATCCTC GTCTCTGCAA GAAAGCCAAA GCCCCTGAAG 
     2041 ACTGTGAGGA TGGAGAGCTT CCATATGGCT GGGAGAAAAT AGAGGACCCT CAGTATGGAA 
     2101 CATACTATGT TGATCACCTC AACCAGAAAA CTCAGTTTGA AAATCCAGTG GAGGAAGCGA 
     2161 AAAGGAAAAA GCAGATAGGA CAGGCTGAAA CTCATTCCGC AAAAACAGAT GTGGAAAGAG 
     2221 CACACTTTAC CCGGGACCCA TCCCAACTTA AAGGTGTCCT TGTTCGAGCA TCACTGAAAA 
     2281 AAAGCACAAT GGGCTTTGGT TTCACCATTA TAGGTGGAGA TCGGCCTGAT GAATTTCTAC 
     2341 AAGTAAAAAA TGTGCTCAAA GACGGTCCCG CTGCTCAGGA TGGGAAAATG GCACCAGGTG 
     2401 ATGTTATTGT AGACATCAAT GGCAACTGTG TCCTTGGTCA CACCCATGCA GATGTTGTCC 
     2461 AGATGTTTCA ATTGGTACCT GTCAACCAGT ATGTCAACCT TACTTTATGC CGTGGTTATG 
     2521 CACTTCCTGA CGACAGTGAA GACCCTGTTG TGGACATTGT TGCTGCTACC CCTGTTATCA 
     2581 ATGGACAATC ATTAGCAAAG GGAGAGGCGT GCATGAGTAC TCAGGATTTT AAACTGGGAG 
     2641 CAATGGTTTT GGATCAGAAT GGAAAATCAG GAAAACTTTT GTCCAGTGAT CGTCTCAATG 
     2701 GCCCATCAGA TTCAAATGAG CAAAGAGCAT CCCTGGCTTC ATCAGGCAGC TCCCAGCCTG 
     2761 AACTAGTGAC TATCCCGCTG GTTAAGGGTC CCAAAGGCTT CGGGTTTGCA ATTGCCGATA 
     2821 GCCCAACTGG GCAGAAGGTG AAAATGATAC TGGATAGCCA GTGGTGTCAA GGCCTTCAAA 
     2881 AAGGGGATAT CATCAAAGAA ATTTACCATC AGAATGTGCA GAATTTAACA CATCTCCAAG 
     2941 TCGTAGAAGT ACTGAAACAG TTCCCAGTAG GTGCAGATGT CCCGTTGCTT ATCTTAAGAG 
     3001 GAGGTCCTTG TTCACCAACT AAAACTGCCA AAATGAAAAC AGATACAAAA GAAACTTCAG 
     3061 GAAGTTTGGA GACTATAAAT GAGCCTACTC CCCAGCCTAT GCCTTTTCCA CCCAGCATAA 
     3121 TCAGATCAGG ATCCCCGAAA TTGGATCCTT CTGAAGTCTA CCTGAAATCT AAGACTTTAT 
     3181 ATGAAGATAA ACCACCCAAC ACCAAAGACC TAGATGTCTT TCTTCGGAAA CAAGAATCGG 
     3241 GGTTTGGCTT CAGGGTGCTG GGAGGAGATG GACCTGACCA GTCTATATAT ATTGGGGCCA 
     3301 TCATTCCCCT GGGAGCAGCG GAAAAGGATG GTAGGCTCCG TGCTGCTGAT GAATTGATGT 
     3361 GTATTGATGG GATTCCTGTT AAAGGGAAAT CACATAAGCA AGTCTTGGAC TTAATGACCA 
     3421 CTGCTGCTCG AAATGGCCAC GTGTTACTCA CTGTGAGGAG GAAGATCTTC TATGGAGAGA 
     3481 AACAACCCGA GGATGAAAGC CCTCAAGCCT TCTCACAGAG TGGATCTCCT CGCCTAAATC 
     3541 GGACAGAGCT TCCAACCAGG TCTGCCCCAC AGGAGTCCTA TGATGTTATC TTACAGAGGA 
     3601 AAGAAAATGA AGGGTTTGGT TTTGTCATCC TCACCTCTAA AAGCAAGCCG CCCCCAGGAG 
     3661 TTATTCCTCA TAAAATTGGC CGGGTCATAG ATGGAAGTCC AGCTGATCGT TGTGGAAGAC 
     3721 TGAAAGTTGG AGACCACATC TCTGCGGTGA ATGGGCAGTC CATTGTTGAC CTGTCCCATG 
     3781 ATAATATTGT TCAGCTCATC AAAGATGCTG GGGTCACCGT CACGCTGACA GTGGTTGCTG 
     3841 AAGAAGAGCA TCATGGTCCA CCATCAGGAA CAAACTCAGC CAGGCAGAGC CCAGCACTGC 
     3901 AGCACAGACC CATGGGACAG GCGCAGGCCA CCCACATACC TGGGGACAGA ACTGCCCTAG 
     3961 AAGGTGAAGT TGGGAAAGAT GTCTGCAGCT CTTACAGACA CTCCTGGTCT GACCACAAGC 
     4021 ACCTTGCACA GCCGGACACC GCGGTGATTT CCGTGGTGGG CAGTCGGCAC AGTCAGAGCC 
     4081 TTGGGTGTTA CCCTGTGGAG CTGGAGAGAG GCCCTCGAGG CTTTGGGTTC AGCCTCCGAG 
     4141 GAGGGAAGGA GTACAACATG GGGCTGTTCA TCCTTCGCCT TGCTGAGGAC GGGCCTGCCA 
     4201 TCAAAGACGG CAGAATTCAC GTTGGTGACC AGATCGTGGA AATCAATGGG GAACCTACAC 
     4261 AAGGCATCAC ACACACGCGA GCCATTGAGC TCATTCAGGC TGGCGGGAAT AAAGTCCTCC 
     4321 TCCTTCTGAG GCCAGGAACT GGCTTGATAC CTGATCATGG TGATTGGGAT ATTTATAGTC 
     4381 CTTCATCTTC CAATGTCATT TATGATGAAC AGCCACCACC TCTCCCCTCT TCACATTCTG 
     4441 CTGCCACATT TGAAGAGTCT CATGTGCCAG TAACTGAAGA CTCTCTGATT AGAGTTCAGA 
     4501 CATGTGAAAA GGCAGAAGAA TTAAAGGACA CTGTACAAGA AAAGAAAAGC ACTTTAAATG 
     4561 GAAGCCAGCC TGAGATGAAG TATCAGTCTG TCCAGAAAAA TGTGAGTAAG AAGGATCCAT 
     4621 CCAGAAGTCA TGGGCATGGT GACAAGAATC TACCTAAAGG AGAAAATGGT GTTACACGGA 
     4681 GAGGTAGATC TGCTAGTCCC AAAAAGTCAG TTAATCGACA TTCAGAGGAA CATCTGGAAA 
     4741 AGATTCCCAG GCCCCTTAGA AGTGACCCCA AAGGAAAATC TAGAGACCGG TCACTCAGCC 
     4801 CTAGGAAAGG AGAAAATAAA GGTCAGGTCA CCATTAAGGC GGGCTCTGGA CAAGATCCCT 
     4861 GTAGAAAAGA CAGAGGACGG TCTTCTAGCC CCAGGAAGCA GCAAAAGATT GGAGGCAACA 
     4921 GCCTGTCAAA CACTGAAGGC AAATTATCAG AGGCTGGGAG CCGAAGAGCA GCAGGCCTCT 
     4981 CTAGTGACAG CCCTGAACAG CTCCCAGAGG GGAAAGAAAA GTCAGGGGTC AGCAGGAAAG 
     5041 ATCTGAAGCT CAGTCAGCTG GGGAAAAACA GAACAAGGTC TCCAGAGAAA AGGAGCAGCA 
     5101 AAGTTGATGA AGCGTCTCTC CCATCCAAAA AGACTAGTGA CACTGCTAGT AGGGTAGTGT 
     5161 CTGAAAAGGA GAAAGGAAGG AAGCCAGGCA CAGGAGAAAG AAGTAGAGAC AAAACAGGAG 
     5221 AAAGCGTCCA GACCTCAGCA AAGCCGCTCA CGCAGGAAGC GGGAGAGAAG ATGGCATTAA 
     5281 GCAAAGCATC AGAGGTCACT GACAGAGGCA AGGAGCGTGC AGGGGGCGCA CCTGAGAGTA 
     5341 GCTCTCCAGT CAAGAAAGCA CCCATCACTC CAGGACCCTG GAGGGTGCCA CGTGCAAATA 
     5401 AAGTCACAGG CACTGCTGGC ATGGCTGACA AACAGCTGAC GCGTACGCGG CCGCTCGAGC 
     5461 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5521 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5581 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5641 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5701 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5761 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5821 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5881 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5941 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     6001 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     6061 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6121 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6181 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6241 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6301 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6361 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6421 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6481 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6541 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6601 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6661 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6721 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6781 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6841 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6901 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6961 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     7021 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     7081 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7141 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7201 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7261 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7321 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7381 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7441 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7501 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7561 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7621 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7681 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7741 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7801 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7861 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7921 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7981 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     8041 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     8101 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8161 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8221 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8281 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8341 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8401 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8461 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8521 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8581 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8641 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8701 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8761 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8821 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8881 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8941 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     9001 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     9061 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9121 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9181 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9241 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//