LOCUS       RR213776                8196 bp    DNA     circular  SYN 
DEFINITION  Atp8b2 (Myc-DDK-tagged ORF) - Rat Atpase, class I, type 8B, member 2 (Atp8b2), (10 ug)
ACCESSION   NM_001024798
VERSION     NM_001024798.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4353..4444 
                     /label="Myc-DDK"  
     misc_feature    4474..5059 
                     /label="PolyA signal"  
     misc_feature    4531..4550 
                     /label="Restriction Enzyme"  
     misc_feature    5335..6007 
                     /label="ColE1"  
     misc_feature    6437..7231 
                     /label="Kan/Neo"  
     misc_feature    7266..7568 
                     /label="SV40 ori"  
     misc_feature    7654..8131 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4335..4341 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4335 
                     /label="Atp8b2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGACGTCG GTGGGCACTA ACCGAGCCCG GGGAAACTGG GAACAACCTC 
     1081 AAAACCAAAA CCAGACACAG CACAAGCAGC GGCCACAGGC CACTGCAGAG CAAATTCGAC 
     1141 TTGCACAGAT GATTTCGGAC CATAATGATG CTGACTTTGA GGAGAAGGTG AAACAATTGA 
     1201 TTGATATCAC AGGCAAAAAC CAGGATGAAT GTGTGATTGC TTTGCATGAC TGCAATGGAG 
     1261 ATGTCAACAG AGCCATCAAT GTTCTCCTGG AAGGAAATCC AGACACGCAT TCCTGGGAGA 
     1321 TGGTCGGGAA GAAGAAAGGA GTCTCAGGAC AGAAGGATGG TGGACAAACT GAATCTAATG 
     1381 AAGAAGGAAA AGAAAATCGA GACCGGGACA GAGACTATAG TCGACGACGT GGTGGGCCAC 
     1441 CAAGACGGGG GAGAGGTGCC AGCCGTGGAC GAGAGTTTCG GGGCCAGGAA AATGGATTGG 
     1501 ATGGCACTAA GAGTGGTGGA CCTTCTGGAA GAGGCACAGA CAGAGGGAGA AGAGGCCGTG 
     1561 GCCGGGGCAG AGGTAGTTCT GGTAGACGAG GAGGAAGGTT TTCTGCTCAA GGAATGGGAA 
     1621 CCTTTAACCC TGCTGATTAT GCAGAGCCAG CCAACACTGA TGATAACTAT GGCAGTAGCA 
     1681 GTGGCAATAC ATGGAACAAC ACTGGCCACT TTGAACCAGA TGATGGAACA AGACTTGATT 
     1741 TCATTGGGGT TGAGGGGTCA AATTATCCCC GAAAATTTGA GACTGCTCCT GGTGCATGGA 
     1801 GGACTGCAAC AGAAGAGTGG GGAACTGAAG ATTGGAATGA AGATCTTTCT GAGACCAAGA 
     1861 TCTTTACTGC CTCTAATGTG TCTTCAGTGC CTCTGCCTGC GGAGAATGTG ACAATCACTG 
     1921 CTGGTCAGAG AATTGATCTT GCTGTTCTGT TGGGGAAGAC ACCGTCTTCA ATGGAGAATG 
     1981 ATTCATCTAA TCTGGATCCA TCTCAGGCTC CTTCCCTGGC CCAGCCTCTG GTATTCAGTA 
     2041 ATTCGAAGCA GAATGCCATA TCACAGCCTG CTTCAGGGAA TACATTTTCT CATCATAGCA 
     2101 TGGTGAGCAT GTTGGGGAAA GGATTTGGTG ATGTTGGGGA AGCTAAAGGT GGCAGTACAA 
     2161 CAGGCTCTCA GTTCTTGGAG CAATTCAAGA CTGCACAGGC CCTGGCTCAA CTGGCAGCTC 
     2221 AACATTCTCA GTCTGGAAGC ACCACCACCT CCTCTTGGGA TATGGGCTCA ACAACACAAT 
     2281 CACCATCACT GGTGCAGTAT GATTTGAAGA GTGCAAATGA TTCAACAGTG CACAGTCCTT 
     2341 TTACAAAGCG CCAGGCATTC ACCCCATCAT CAACCATGAT GGAGGTGTTC CTTCAGGAGA 
     2401 AGCCACCTGC AGTGGCTACC TCGACAGCTG CACCTCCTCC CCCCTCTTCT CCTCTGCCAA 
     2461 GCAAATCCAC CTCGGCTCCA CAGATGTCTC CGGGGTCTTC GGACAACCAA TCCTCCAGCC 
     2521 CTCAGCCGGC TCAACAGAAA CTGAAACAGC AAAAGAAGAA AACCTCCTTG ACGTCTAAGA 
     2581 TTCCTGCTCT GGCTGTGGAG ATGCCTGGCT CAGCAGATAT CTCAGGGCTA AATCTGCAGT 
     2641 TTGGGGCATT GCAATTTGGG TCAGAGCCTG TCCTTTCTGA TTATGAGTCC ACCCCCACCA 
     2701 CGAGCGCCTC TTCAAGCCAG GCTCCCAGTA GCCTGTATAC CAGCACGGCC AGTGAGTCTT 
     2761 CATCTACAGT TTCATCTAAT CAGAGTCAGG AGTCTGGTTA TCAGAGTGGC CCAATTCAGT 
     2821 CGACAACTTA TACCTCCCAA AATAATGCTC AGGGCCCTCT ATATGAACAG AGATCCACAC 
     2881 AGACACGGCG GTATCCCAGC TCCATCTCTT CATCACCCCA AAAGGACCTG ACTCAGGCTA 
     2941 AGAATGGTTT CAGCTCTGTG CAGGCCACGC AGTTACAGAC CACGCAATCT GTTGAAGGTG 
     3001 CTACAGGCTC TGCAGTGAAA TCTGAATCAC CTTCCACTTC TAGTATACCC TCTCTCAATG 
     3061 AAACAGTACC TGCAGCTTCC TTACTGACGA CAGCCAGTCA GCATTCATCC TCCTTAAGTG 
     3121 GCTTGAGCCA TAGTGAGGAG ATTCCAAATA CCACCACCAC ACAACATAGC AGTGCATTAT 
     3181 CTACACAGCA GAATACCCTT TCTTCATCAA CATCTTCTGG GCGTACTTCA ACATCTACTC 
     3241 TTTTGCATAC AAGTGTGGAG AGTGAAGCGA ATCTGCATTC TTCCTCCAGC ACTTTCTCTA 
     3301 CCACGTCCAG CACTGTCTCT GCACCTCCCC CAGTGGTCAG TGTCTCCTCC AGTCTGAATA 
     3361 GTGGCAGTAG CCTGGGCCTT AGCCTGGGCA GCAACTCTAC TGTCACAGCC TCAACTCGAA 
     3421 GCTCAGTTGC TACAACTTCA GGAAAAGCTC CTCCCAACCT TCCTCCTGGA GTCCCGCCAT 
     3481 TGTTGCCTAA TCCATATATT ATGGCTCCAG GGCTGTTACA TGCCTACCCG CCACAAGTAT 
     3541 ATGGTTATGA TGATTTACAG ATGCTCCAGA CAAGATTTCC ATTGGATTAC TACAGTATCC 
     3601 CGTTTCCCAC ACCCACCACT CCACTTACTG GGAGGGATGG TAGCCTGGCC AGCAACCCTT 
     3661 ACTCTGGCGA CCTCACCAAG TTCGGCCGCG GGGATGCCTC CTCCCCAGCC CCAGCCACAA 
     3721 CCTTGGCCCA ACCCCAACAG AACCAGACGC AGACTCACCA CACCACGCAG CAGACATTCC 
     3781 TGAACCCGGC GCTGCCTCCT GGCTATAGTT ACACCAGCCT GCCATACTAC ACAGGGGTCC 
     3841 CGGGCCTCCC CAGCACCTTC CAGTATGGGC CTGCTGTGTT CCCTGTGGCT CCTACCTCTT 
     3901 CCAAGCAGCA TGGTGTGAAT GTCAGTGTGA ATGCCTCGGC CACCCCTTTC CAACAACCAA 
     3961 GTGGATATGG GTCTCATGGA TACAACACTG GTGTGTCAGT CACCTCCAGT AACACGGGCG 
     4021 TGCCAGATAT CTCGGGTTCT GTGTACTCCA AAACCCAGCA GTCCTTTGAG AAACAAGGTT 
     4081 TTCATTCCGG TACTCCTGCT GCCTCCTTCA ACTTGCCTTC AGCCCTAGGA AGTGGAGGGC 
     4141 CCATCAATCC AGCCACAGCT GCTGCCTACC CACCTGCCCC CTTTATGCAC ATTCTGACCC 
     4201 CACATCAGCA GCCACACTCT CAGATCCTTC ACCATCACCT GCAGCAGGAT GGCCAGCTAC 
     4261 CATATTTGCA GATGATTCTC TGTTGCCAGC GCCAGCAGGA GGAGCAGGAT ATCCTCACTC 
     4321 TCGTCGATGA CCAGCTTGGT GAAACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4381 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4441 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4501 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4561 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4621 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4681 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4741 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4801 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4861 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4921 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4981 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5041 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5101 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5161 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5221 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5281 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5341 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5401 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5461 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5521 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5581 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5641 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5701 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5761 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5821 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5881 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5941 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6001 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6061 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6121 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6181 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6241 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6301 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6361 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6421 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6481 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6541 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6601 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6661 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6721 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6781 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6841 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6901 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6961 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7021 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7081 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7141 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7201 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7261 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7321 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7381 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7441 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7501 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7561 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7621 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7681 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7741 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7801 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7861 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7921 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7981 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8041 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8101 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8161 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//