LOCUS       RR213734                8136 bp    DNA     circular  SYN 
DEFINITION  Cacna2d3 (Myc-DDK-tagged ORF) - Rat calcium channel, voltage-dependent, alpha2/delta subunit 3 (Cacna2d3), (10 ug)
ACCESSION   NM_175595
VERSION     NM_175595.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4293..4384 
                     /label="Myc-DDK"  
     misc_feature    4414..4999 
                     /label="PolyA signal"  
     misc_feature    4471..4490 
                     /label="Restriction Enzyme"  
     misc_feature    5275..5947 
                     /label="ColE1"  
     misc_feature    6377..7171 
                     /label="Kan/Neo"  
     misc_feature    7206..7508 
                     /label="SV40 ori"  
     misc_feature    7594..8071 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4275..4281 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4275 
                     /label="Cacna2d3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCGGGCCG GGCTCGCTGT GCTGCGCGTC CCGGGGGGCC TCGGCGCTCC 
     1081 TAGCCACAGC GCTTCTCTAC GCCGCGCTGG GGGACGTGGT GCGCTCCGAG CAGCAGATCC 
     1141 CGCTCTCCGT AGTGAAGCTC TGGGCCTCCG CTTTTGGTGG GGAGATAAAA TCCATTGCTG 
     1201 CCAAGTACTC GGGTTCCCAG CTTCTGCAAA AGAAATACAA AGAGTATGAG AAAGACGTTG 
     1261 CCATTGAAGA AATCGACGGT CTCCAACTGG TGAAAAAGCT GGCCAAGAAC ATGGAAGAGA 
     1321 TGTTTCACAA GAAGTCCGAG GCAGTGCGGC GTCTCGTGGA GGCTGCAGAG GAAGCACACC 
     1381 TGAAGCATGA ATTTGACGCC GACTTGCAGT ATGAATACTT CAATGCCGTG CTGATCAACG 
     1441 AGAGAGACAA AGATGGGAAC TTTTTGGAAT TGGGAAAGGA GTTCATCTTG GCCCCCAATG 
     1501 ACCATTTTAA TAATTTGCCT GTGAACATCA GTCTGAGTGA TGTCCAAGTG CCAACGAACA 
     1561 TGTACAACAA AGATCCTGCC ATAGTCAATG GAGTGTATTG GTCTGAATCT CTAAACAAAG 
     1621 TTTTTGTAGA CAACTTCGAT CGGGACCCGT CTCTCATATG GCAGTACTTT GGAAGTGCAA 
     1681 AGGGCTTTTT CAGACAGTAC CCAGGGATTA AATGGGAACC AGACGAGAAT GGAGTCATTG 
     1741 CCTTTGACTG CAGGAACAGG AAATGGTACA TCCAGGCAGC AACTTCTCCA AAGGATGTGG 
     1801 TCATTTTGGT GGACGTCAGC GGGAGCATGA AAGGACTCCG CCTGACCATC GCCAAGCAAA 
     1861 CAGTCTCCTC GATACTGGAC ACCCTGGGCG ATGATGACTT CTTCAACATC ATCACGTATA 
     1921 ACGAAGAGCT TCACTATGTG GAACCTTGTC TGAATGGAAC ACTGGTTCAA GCGGACAGGA 
     1981 CCAACAAGGA GCACTTCAGG GAGCATTTGG ACAAACTTTT TGCCAAAGGG ATTGGAATGC 
     2041 TCGATATTGC GCTGAACGAG GCCTTCAATG TACTGAGCGA TTTCAACCAC ACCGGACAAG 
     2101 GAAGCATTTG CAGCCAGGCC ATTATGCTCA TAACCGACGG GGCAGTGGAC ACCTACGACA 
     2161 CCATCTTTGC AAAGTACAAT TGGCCAGAGC GAAAGGTTCG AATCTTCACT TACCTCATTG 
     2221 GACGAGAGGC TGCTTTTGCA GACAATCTCA AGTGGATGGC TTGTGCTAAC AAAGGATTTT 
     2281 TCACCCAGAT CTCCACCTTG GCTGATGTGC AGGAAAATGT CATGGAATAC CTCCATGTAC 
     2341 TCAGTCGACC CAAAGTCATC GACCAGGAAC ATGATGTGGT GTGGACCGAA GCGTACATCG 
     2401 ACAGCACTCT TGCTGATGAT CAGGGCCTCG TCTTGATGAC CACAGTGGCC ATGCCTGTGT 
     2461 TTAGTAAGCA GAACGAAACT AGGTCAAAGG GCATTCTTCT GGGTGTGGTT GGCACAGATG 
     2521 TCCCAGTAAA AGAGCTTCTG AAGACCATCC CCAAATACAA GTTAGGAATT CATGGTTATG 
     2581 CCTTTGCCAT CACGAATAAT GGATACATCT TGACACACCC GGAGCTCAGG CCCCTGTATG 
     2641 AAGAAGGGAA AAAGCGAAGG AAGCCTAATT ACAGTAGTGT GGATCTCTCG GAAGTCGAGT 
     2701 GGGAAGATCG GGATGATGTG TTACGAAATG CCATGGTGAA TCGGAAGACT GGGAAATTCT 
     2761 CCATGGAAGT GAAGAAGACT GTGGACAAAG GGAAACGGGT TTTGGTGATG ACCAATGACT 
     2821 ACTACTACAC AGACATCAAG GGTGCTCCTT TCAGTTTAGG TGTGGCGCTC TCCAGGGGCC 
     2881 ACGGGAAATA CTTCTTCCGA GGGAATGTAA CCATTGAAGA AGGGCTCCAT GACTTAGAAC 
     2941 ATCCTGACGT GTCCTTGGCA GATGAATGGT CCTACTGCAA CACTGATCTG CACCCAGAGC 
     3001 ACCGCCATCT CTCTCAACTG GAAGCGATTA AGCTCTACCT CAAAGGCAAG GAGCCTCTGC 
     3061 TTCAATGTGA CAAAGAATTG ATTCAAGAAG TCCTTTTTGA TGCTGTGGTA AGCGCCCCTA 
     3121 TCGAAGCCTA TTGGACCAGC CTGGCCCTCA ACAAATCTGA GAATTCTGAC AAGGGTGTAG 
     3181 AGGTCGCCTT CCTCGGCACT CGCACAGGCC TCTCAAGAAT CAACCTGTTT GTGGGGGCTG 
     3241 AACAGCTCAC CAATCAGGAC TTTCTGAAGG CTAGAGACAA AGAGAACATT TTCAACGCAG 
     3301 ATCATTTCCC TCTCTGGTAC AGAAGAGCTG CCGAGCAGAT TCCAGGAAGT TTTGTCTACT 
     3361 CCATCCCCTT CAGCACAGGA ACGGTCAACA AAAGCAATGT GGTGACAGCA AGTACCTCCA 
     3421 TCCAACTCCT GGATGAGCGA AAATCTCCTG TGGTGGCAGC TGTAGGCATT CAGATGAAAC 
     3481 TTGAATTCTT CCAAAGGAAG TTCTGGACGG CCAGCAGACA GTGTGCCTCC CTGGATGGTA 
     3541 AATGCTCCAT AAGCTGCGAC GATGAGACTG TGAACTGTTA CCTCATAGAC AATAACGGGT 
     3601 TCATTCTGGT GTCTGAAGAC TACACACAGA CTGGAGATTT TTTTGGTGAG GTCGAAGGAG 
     3661 CTGTCATGAA CAAGTTGTTA ACAATGGGCT CCTTTAAAAG AATAACCTTG TATGACTACC 
     3721 AAGCCATGTG TAGAGCCAAC AAGGAGAGTA GTGACAGTGC CCACGGACTC CTGGACCCCT 
     3781 ATAAGGCCTT CCTCTCTGCA GCCAAGTGGA TAGTGACGGA ACTTGTCTTG TTCCTGGTGG 
     3841 AGTTTAACCT TTGCAGTTGG TGGCACTCTG ACATGACAGC TAAAGCCCAG AAACTGAAAC 
     3901 AGACCCTGGA GCCTTGTGAT ACTGAATACC CAGCCTTTGT TTCCGAACGC ACCATCAAGG 
     3961 AGACCACAGG GAACATTGCT TGTGAAGACT GCTCCAAGTC CTTTGTCATC CAGCAAATCC 
     4021 CAAGTAGCAA TCTGTTCATG GTGGTGGTGG ACAGTAGCTG TCTCTGTGAG TCTGTGGCTC 
     4081 CTATCACCAT GGCACCCATT GAAATCAGGT ATAATGAATC CCTTAAGTGT GAACGGTTAA 
     4141 AGGCTCAGAA GATCAGACGA CGTCCGGAAT CCTGCCACGG CTTCCATCCT GAGGAGAATG 
     4201 CGAGAGAGTG TGGGGGTGCA TCAAGTCTCC AGGCCCAGGT GGCCTTGCTG CTGCTCCCCC 
     4261 TGGTTTCGAG TCTCTTCTCA AGGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     4321 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     4381 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     4441 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     4501 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     4561 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     4621 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4681 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4741 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4801 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4861 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4921 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4981 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5041 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     5101 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     5161 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     5221 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     5281 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     5341 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     5401 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     5461 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     5521 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     5581 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     5641 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5701 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5761 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5821 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5881 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5941 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6001 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6061 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     6121 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     6181 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     6241 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     6301 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     6361 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     6421 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     6481 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     6541 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     6601 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6661 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6721 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6781 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6841 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6901 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6961 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7021 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7081 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     7141 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     7201 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     7261 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     7321 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     7381 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7441 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7501 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     7561 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     7621 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7681 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7741 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7801 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7861 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7921 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7981 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8041 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     8101 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//