LOCUS       RR213722                7698 bp    DNA     circular  SYN 
DEFINITION  Ap2a2 (Myc-DDK-tagged ORF) - Rat adaptor-related protein complex 2, alpha 2 subunit (Ap2a2), (10 ug)
ACCESSION   NM_031008
VERSION     NM_031008.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3855..3946 
                     /label="Myc-DDK"  
     misc_feature    3976..4561 
                     /label="PolyA signal"  
     misc_feature    4033..4052 
                     /label="Restriction Enzyme"  
     misc_feature    4837..5509 
                     /label="ColE1"  
     misc_feature    5939..6733 
                     /label="Kan/Neo"  
     misc_feature    6768..7070 
                     /label="SV40 ori"  
     misc_feature    7156..7633 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3837..3843 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3837 
                     /label="Ap2a2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCCGGCCGTA TCCAAGGGGG AGGGGATGCG AGGCCTAGCG GTCTTCATCT 
     1081 CCGATATCCG TAACTGTAAA AGTAAAGAAG CTGAAATAAA GAGGATAAAT AAGGAGCTGG 
     1141 CAAATATTAG ATCAAAATTT AAAGGTGACA AGGCTCTTGA TGGCTACAGT AAGAAGAAGT 
     1201 ATGTCTGCAA GTTACTCTTC ATCTTTCTCC TTGGTCATGA CATTGACTTT GGACACATGG 
     1261 AAGCTGTGAA TCTTCTGAGC TCAAACAGAT ACACGGAAAA GCAGATCGGC TACCTTTTCA 
     1321 TCTCTGTATT GGTGAATTCA AATAGTGAAC TGATCCGGTT GATTAACAAT GCCATCAAGA 
     1381 ATGACCTGGC CAGCCGCAAT CCTACATTTA TGGGTCTGGC CCTGCACTGC ATCGCCAATG 
     1441 TGGGTAGCCG TGAGATGGCA GAGGCCTTCG CTGGAGAGAT CCCCAAGATC CTGGTGGCAG 
     1501 GAGATACCAT GGACAGTGTG AAGCAGAGCG CAGCCCTGTG CTTGTTGCGC CTGTACAGGA 
     1561 CGTCACCTGA CCTAGTTCCT ATGGGCGACT GGACATCCCG TGTGGTACAC CTCCTCAATG 
     1621 ACCAGCACTT GGGTGTAGTG ACTGCCGCAA CTAGCCTCAT CACCACACTG GCCCAGAAGA 
     1681 ATCCTGAGGA GTTCAAGACC TCTGTCTCTC TAGCTGTCTC TCGACTGAGC AGAATTGTGA 
     1741 CGTCCGCGTC AACAGATCTT CAGGATTATA CTTACTACTT TGTCCCGGCT CCTTGGCTGT 
     1801 CAGTCAAACT TCTGAGACTG CTGCAGTGCT ACCCACCCCC AGAAGACCCT GCGGTGCGTG 
     1861 GCCGCCTGAC TGAGTGCTTG GAGACCATCC TGAATAAGGC CCAGGAGCCC CCCAAGTCCA 
     1921 AGAAAGTCCA GCATTCAAAT GCCAAGAACG CTGTGCTGTT TGAGGCCATC AGCCTCATCA 
     1981 TCCACCATGA CAGTGAGCCC AACCTCCTGG TCCGTGCCTG CAACCAGCTG GGCCAGTTCC 
     2041 TGCAGCATCG GGAGACAAAC CTGCGCTACC TGGCTCTGGA GAGCATGTGC ACACTGGCCA 
     2101 GCTCTGAGTT CTCTCACGAG GCTGTCAAGA CCCACATCGA GACTGTCATC AATGCCTTGA 
     2161 AGACGGAACG AGATGTGAGT GTGCGGCAGC GGGCAGTGGA CCTGCTCTAC GCCATGTGCG 
     2221 ACCGAAGCAA CGCCCAGCAG ATTGTGGCTG AGATGCTGAG CTACCTAGAA ACAGCTGACT 
     2281 ACTCCATCCG GGAGGAAATT GTGCTGAAGG TGGCCATCCT TGCTGAGAAG TATGCAGTGG 
     2341 ACTACACTTG GTATGTGGAC ACCATCCTCA ACCTCATCCG CATTGCAGGC GACTATGTGA 
     2401 GCGAAGAGGT GTGGTACCGC GTCATCCAGA TTGTCATCAA TCGCGATGAT GTGCAGGGTT 
     2461 ATGCTGCCAA GACGGTGTTT GAGGCATTGC AGGCTCCAGC ATGTCATGAG AACTTGGTCA 
     2521 AAGTGGGCGG CTACATCCTG GGCGAGTTTG GAAACTTGAT AGCGGGAGAC CCACGATCCA 
     2581 GCCCTCTGAT CCAGTTCAAC CTGCTCCACT CCAAGTTCCA CTTGTGCAGC GTCCCCACTC 
     2641 GGGCACTCCT GCTGTCCACC TACATCAAGT TCGTGAACCT CTTTCCAGAG GTAAAGGCTA 
     2701 CCATTCAGGA CGTGCTGCGC AGTGACAGCC AACTGAAGAA CGCAGATGTG GAGCTGCAGC 
     2761 AGCGGGCTGT GGAGTACCTG CGACTGAGTA CTGTTGCCAG CACTGATATC CTGGCAACTG 
     2821 TCCTGGAAGA AATGCCACCC TTCCCTGAGC GTGAGTCCTC CATCTTGGCC AAGCTGAAGA 
     2881 AGAAGAAGGG CCCAAGCACA GTGACTGACC TGGAGGAGAC CAAGCGTGAG CGAAGCATCG 
     2941 ATGTGAATGG GGGCCCCGAA CCCGTTCCAG CCAGCACCAG TGCTGCGTCC ACACCTTCTC 
     3001 CATCAGCAGA CCTGCTGGGT CTGGGGGCCG TTCCCCCTGC TCCCACAGGG CCCCCTCCCA 
     3061 CCTCTGGCGG TGGGTTACTG GTGGACGTGT TCTCAGACTC AGCTTCTGCA GTTGCACCTC 
     3121 TTGCACCCGG CTCTGAAGAC AACTTTGCCA GGTTTGTTTG TAAAAACAAT GGTGTGTTGT 
     3181 TTGAAAACCA GCTGCTTCAA ATTGGACTGA AGTCAGAATT TCGGCAGAAT TTAGGTCGAA 
     3241 TGTTCATATT TTATGGTAAC AAGACCTCCA CGCAGTTCCT AAACTTCACT CCAACACTAA 
     3301 TCTGTGCTGA CGACCTTCAG ACTAATCTGA ACCTGCAGAC TAAGCCCGTG GACCCAACTG 
     3361 TGGATGGGGG CGCACAGGTG CAACAGGTGG TCAACATTGA GTGTATATCT GATTTCACAG 
     3421 AGGCACCTGT CCTCAACATC CAGTTCAGGT ATGGTGGTAC CTTCCAGAAT GTGTCTGTTA 
     3481 AGCTTCCCAT CACTCTCAAC AAATTTTTCC AGCCCACAGA AATGGCTTCT CAGGATTTCT 
     3541 TTCAACGTTG GAAGCAGTTG AGCAATCCAC AGCAAGAAGT GCAGAATATC TTCAAAGCAA 
     3601 AGCATCCGAT GGACACAGAA ATCACTAAAG CGAAGATTAT TGGATTTGGT TCTGCGCTCC 
     3661 TGGAAGAAGT TGATCCAAAC CCTGCAAATT TTGTGGGTGC TGGCATAATA CATACCAAAA 
     3721 CCACCCAGAT CGGCTGCTTG TTGCGCCTGG AGCCAAACCT GCAAGCTCAG ATGTACAGAC 
     3781 TCACCTTGCG TACCAGCAAA GACACCGTCT CTCAGAGACT ATGTGAATTG CTGTCGGAAC 
     3841 AGTTCACGCG TACGCGGCCG CTCGAGCAGA AACTCATCTC AGAAGAGGAT CTGGCAGCAA 
     3901 ATGATATCCT GGATTACAAG GATGACGACG ATAAGGTTTA AACGGCCGGC CGCGGTCATA 
     3961 GCTGTTTCCT GAACAGATCC CGGGTGGCAT CCCTGTGACC CCTCCCCAGT GCCTCTCCTG 
     4021 GCCCTGGAAG TTGCCACTCC AGTGCCCACC AGCCTTGTCC TAATAAAATT AAGTTGCATC 
     4081 ATTTTGTCTG ACTAGGTGTC CTTCTATAAT ATTATGGGGT GGAGGGGGGT GGTATGGAGC 
     4141 AAGGGGCAAG TTGGGAAGAC AACCTGTAGG GCCTGCGGGG TCTATTGGGA ACCAAGCTGG 
     4201 AGTGCAGTGG CACAATCTTG GCTCACTGCA ATCTCCGCCT CCTGGGTTCA AGCGATTCTC 
     4261 CTGCCTCAGC CTCCCGAGTT GTTGGGATTC CAGGCATGCA TGACCAGGCT CAGCTAATTT 
     4321 TTGTTTTTTT GGTAGAGACG GGGTTTCACC ATATTGGCCA GGCTGGTCTC CAACTCCTAA 
     4381 TCTCAGGTGA TCTACCCACC TTGGCCTCCC AAATTGCTGG GATTACAGGC GTGAACCACT 
     4441 GCTCCCTTCC CTGTCCTTCT GATTTTAAAA TAACTATACC AGCAGGAGGA CGTCCAGACA 
     4501 CAGCATAGGC TACCTGGCCA TGCCCAACCG GTGGGACATT TGAGTTGCTT GCTTGGCACT 
     4561 GTCCTCTCAT GCGTTGGGTC CACTCAGTAG ATGCCTGTTG AATTGGGTAC GCGGCCAGCG 
     4621 GCGAGCGGTA TCAGCTCACT CAAAGGCGGT AATACGGTTA TCCACAGAAT CAGGGGATAA 
     4681 CGCAGGAAAG AACATGTGAG CAAAAGGCCA GCAAAAGGCC AGGAACCGTA AAAAGGCCGC 
     4741 GTTGCTGGCG TTTTTCCATA GGCTCCGCCC CCCTGACGAG CATCACAAAA ATCGACGCTC 
     4801 AAGTCAGAGG TGGCGAAACC CGACAGGACT ATAAAGATAC CAGGCGTTTC CCCCTGGAAG 
     4861 CTCCCTCGTG CGCTCTCCTG TTCCGACCCT GCCGCTTACC GGATACCTGT CCGCCTTTCT 
     4921 CCCTTCGGGA AGCGTGGCGC TTTCTCATAG CTCACGCTGT AGGTATCTCA GTTCGGTGTA 
     4981 GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA CGAACCCCCC GTTCAGCCCG ACCGCTGCGC 
     5041 CTTATCCGGT AACTATCGTC TTGAGTCCAA CCCGGTAAGA CACGACTTAT CGCCACTGGC 
     5101 AGCAGCCACT GGTAACAGGA TTAGCAGAGC GAGGTATGTA GGCGGTGCTA CAGAGTTCTT 
     5161 GAAGTGGTGG CCTAACTACG GCTACACTAG AAGAACAGTA TTTGGTATCT GCGCTCTGCT 
     5221 GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG TAGCTCTTGA TCCGGCAAAC AAACCACCGC 
     5281 TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA GCAGATTACG CGCAGAAAAA AAGGATCTCA 
     5341 AGAAGATCCT TTGATCTTTT CTACGGGGTC TGACGCTCAG TGGAACGAAA ACTCACGTTA 
     5401 AGGGATTTTG GTCATGAGAT TATCAAAAAG GATCTTCACC TAGATCCTTT TAAATTAAAA 
     5461 ATGAAGTTTT AAATCAATCT AAAGTATATA TGAGTAACCT GAGGCTATGG CAGGGCCTGC 
     5521 CGCCCCGACG TTGGCTGCGA GCCCTGGGCC TTCACCCGAA CTTGGGGGGT GGGGTGGGGA 
     5581 AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA ATGGGGTCTC GGTGGGGTAT CGACAGAGTG 
     5641 CCAGCCCTGG GACCGAACCC CGCGTTTATG AACAAACGAC CCAACACCGT GCGTTTTATT 
     5701 CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC 
     5761 AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA CTCCAGCATG AGATCCCCGC GCTGGAGGAT 
     5821 CATCCAGCCG GCGTCCCGGA AAACGATTCC GAAGCCCAAC CTTTCATAGA AGGCGGCGGT 
     5881 GGAATCGAAA TCTCGTGATG GCAGGTTGGG CGTCGCTTGG TCGGTCATTT CGAACCCCAG 
     5941 AGTCCCGCTC AGAAGAACTC GTCAAGAAGG CGATAGAAGG CGATGCGCTG CGAATCGGGA 
     6001 GCGGCGATAC CGTAAAGCAC GAGGAAGCGG TCAGCCCATT CGCCGCCAAG CTCTTCAGCA 
     6061 ATATCACGGG TAGCCAACGC TATGTCCTGA TAGCGATCCG CCACACCCAG CCGGCCACAG 
     6121 TCGATGAATC CAGAAAAGCG GCCATTTTCC ACCATGATAT TCGGCAAGCA GGCATCGCCA 
     6181 TGGGTCACGA CGAGATCCTC GCCGTCGGGC ATGCTCGCCT TGAGCCTGGC GAACAGTTCG 
     6241 GCTGGCGCGA GCCCCTGATG CTCTTCGTCC AGATCATCCT GATCGACAAG ACCGGCTTCC 
     6301 ATCCGAGTAC GTGCTCGCTC GATGCGATGT TTCGCTTGGT GGTCGAATGG GCAGGTAGCC 
     6361 GGATCAAGCG TATGCAGCCG CCGCATTGCA TCAGCCATGA TGGATACTTT CTCGGCAGGA 
     6421 GCAAGGTGAG ATGACAGGAG ATCCTGCCCC GGCACTTCGC CCAATAGCAG CCAGTCCCTT 
     6481 CCCGCTTCAG TGACAACGTC GAGCACAGCT GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC 
     6541 GATAGCCGCG CTGCCTCGTC TTGCAGTTCA TTCAGGGCAC CGGACAGGTC GGTCTTGACA 
     6601 AAAAGAACCG GGCGCCCCTG CGCTGACAGC CGGAACACGG CGGCATCAGA GCAGCCGATT 
     6661 GTCTGTTGTG CCCAGTCATA GCCGAATAGC CTCTCCACCC AAGCGGCCGG AGAACCTGCG 
     6721 TGCAATCCAT CTTGTTCAAT CATGCGAAAC GATCCTCATC CTGTCTCTTG ATCGATCTTT 
     6781 GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC TCACTACTTC TGGAATAGCT CAGAGGCCGA 
     6841 GGCGGCCTCG GCCTCTGCAT AAATAAAAAA AATTAGTCAG CCATGGGGCG GAGAATGGGC 
     6901 GGAACTGGGC GGAGTTAGGG GCGGGATGGG CGGAGTTAGG GGCGGGACTA TGGTTGCTGA 
     6961 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7021 CCTGGTTGCT GACTAATTGA GATGCATGCT TTGCATACTT CTGCCTGCTG GGGAGCCTGG 
     7081 GGACTTTCCA CACCCTAACT GACACACATT CCACAGCTGG TTCTTTCCGC CTCAGGACTC 
     7141 TTCCTTTTTC AATATTATTG AAGCATTTAT CAGGGTTATT GTCTCATGAG CGGATACATA 
     7201 TTTGAATGTA TTTAGAAAAA TAAACAAATA GGGGTTCCGC GCACATTTCC CCGAAAAGTG 
     7261 CCACCTGACG CGCCCTGTAG CGGCGCATTA AGCGCGGCGG GTGTGGTGGT TACGCGCAGC 
     7321 GTGACCGCTA CACTTGCCAG CGCCCTAGCG CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT 
     7381 CTCGCCACGT TCGCCGGCTT TCCCCGTCAA GCTCTAAATC GGGGGCTCCC TTTAGGGTTC 
     7441 CGATTTAGTG CTTTACGGCA CCTCGACCCC AAAAAACTTG ATTAGGGTGA TGGTTCACGT 
     7501 AGTGGGCCAT CGCCCTGATA GACGGTTTTT CGCCCTTTGA CGTTGGAGTC CACGTTCTTT 
     7561 AATAGTGGAC TCTTGTTCCA AACTGGAACA ACACTCAACC CTATCTCGGT CTATTCTTTT 
     7621 GATTTATAAG GGATTTTGCC GATTTCGGCC TATTGGTTAA AAAATGAGCT GATTTAACAA 
     7681 AAATTTAACG CGAATTTT
//