LOCUS       RR213718                7893 bp    DNA     circular  SYN 
DEFINITION  Rps6kc1 (Myc-DDK-tagged ORF) - Rat ribosomal protein S6 kinase, polypeptide 1 (Rps6kc1), (10 ug)
ACCESSION   NM_001105984
VERSION     NM_001105984.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4050..4141 
                     /label="Myc-DDK"  
     misc_feature    4171..4756 
                     /label="PolyA signal"  
     misc_feature    4228..4247 
                     /label="Restriction Enzyme"  
     misc_feature    5032..5704 
                     /label="ColE1"  
     misc_feature    6134..6928 
                     /label="Kan/Neo"  
     misc_feature    6963..7265 
                     /label="SV40 ori"  
     misc_feature    7351..7828 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4032..4038 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4032 
                     /label="Rps6kc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCTCTCCT TGGGGTCACA GTGCGGACCT GGCCCGCTTC TACACTGTCA 
     1081 CCGAGCCCCA GCGACACCCT AGGGGCTACA CGATCTATAA GGTTACCGCA CGGGTCGTTT 
     1141 CACGAAGAAA TCCGGAGGAC GTCCAGGAGA TAATTGTGTG GAGAAGATAC AGTGACTTTA 
     1201 AGAAGCTGCA CAGAGAGCTA TGGCAGATTC ACAGGAACGC ATTTCGACAC TCTGAGCTGT 
     1261 TTCCCCCGTT TGCTAAAGGG ACAGTGTTTG GGCGATTTGA CAAAACCGTA ATTGAGGAAC 
     1321 GACGTCAGTG TGCTGAGGAC CTGCTACAGT TCTCTGCCAA CATCCCTGCT CTGTACAACA 
     1381 GCAAGCAGCT CCAGGACTTC TTTAAGGGTG GGATAATTAG TGATGGCTCT GAGTTAATTG 
     1441 GCCCTGCTGA AGCTTACCCA GACTCCCTGG CCAACACCTT TTCTGAGTGT GGTACTGAAG 
     1501 GCTTCTCCAG TGACAGTGAC CTCCTGTCCC TCACTGTTGA TGCGGACTCT CTTGCCGAGG 
     1561 TAGATGATGG AATGGCTTCC CGTCAGGGTT CTCCCAGTAG AGCTTTTGGT CTGAGTGTGT 
     1621 CTGCGGATTC TTCGGCAGTA GGGGCTGTTG CTTCTGACAT TGAACCAAGC AAAGCAGAAG 
     1681 ACCGGGAAAG CCGCAGCCTC TTCCCCAGCA GCTTAAAGCC AAGGCTTGGC AGGAGAGATT 
     1741 ACCTGGAGAA GGCAGGGGAA CTTATCAAAC TGGCCTTAAA GAAGGAGGAG GAAGATGACT 
     1801 ATGAAGCAGC TTCTGACTTT TACAGGAAGG GTGTTGATCT GCTCCTAGAA GGTGTACAAG 
     1861 GAGCACTAAG TTCAAGGCCC CCTTGGAACC TAAGGAGCCC TGCCGAGGAG CTGAAGGCCT 
     1921 TCAGAGTCCT TGGGGTGATT GACAAGGTTT TACTTGTAAT GGACACAAGG ACAGAACAGA 
     1981 CATTCATTTT AAAAGGTCTC AGGAAAAGCA GCGAGTGCAG CAGGAACAGG AAGACCATCA 
     2041 TCCCCCGCTG TGTGCCTAAC ATGGTGTGTC TGCACACATA CATCATCTCG GAGGAGTCGG 
     2101 TGTTTCTCGT GCTGCAGCAC GCCGAAGGTG GCAAACTCTG GTCATATATC AGTAAATTTC 
     2161 TGAACAGAAG CTCTCGAGAA AGCTTGGACA TTAAGGAGGC GAGACCGCCC ATGCCTCCTC 
     2221 GAGTGCGCCT GCAGCAGCCA AGTGCTAGTC CTCAGGGCAG CTACAGTGTT GAGTCCAGAG 
     2281 GGAGCGACAC AGGGAGCATG CTGAAGGTTC TGCCCCTGAA GACTAGCCTC ACCCCGAGTT 
     2341 CTCAGGACGA TAGTAACCAG GAAGATGATG GGCAGGACAG CTCTCCCAAG TGGCCCGATT 
     2401 CGGGCTCCAG CTCAGAGGAA GAGTGCACAG CTGGCTACTT AACGCTGTGC AACGAATATG 
     2461 GGCAAGAAAA GCTTGACCTT ACATCTTTGA ATGAGGAGTC TGTCATGCAG CCTGAAGGGG 
     2521 AGAATGCTGA TAGCCAAGCT GTTAGGAGCT TCCCAGCATC CCTCACCGCT GACAGTGCAA 
     2581 GCCCCAGCAC CCAGCTCAAG TTCTTCCCTG GAGACGAGGG CCTAGAAGCA GTTTGTTCTC 
     2641 CTCGAACATC TGATTCCCTC AGGAGGTCGA AAAACAGTCC TATGGAGTTC TTCCGAATAG 
     2701 ACAGTAAAGA CAGCACAAGT GAGCTCTTGG GACTGGATTC TGGTGAGAAA TTACACAGTC 
     2761 TGAAACCAGA GCCTTTGAAA GCACTCTTTA CCCTTGAAGA TGGAGACAGT CCTTCCCAGA 
     2821 GTTTTGATGC CGGCAAGAGC CAGGGAGAGA GTGAAGCTCA GGACTCCATC AGCAGGGGCT 
     2881 CAGATGACTC CGTCCCGGTT ATCTCATTTA AAGAGGCTGC CTCTGAGGAT GTCTGTAGTG 
     2941 CTGAGGAAGG GAGGCCTGAC CTTCTTGTGA ATCTACCTGG TGAACTGCAG CCAACCAAAG 
     3001 AGACTTCAGC AGTGGACCCT ACTAAGTTTT CACAGGCCAG CATAGGCAGG CTGGAAGCCC 
     3061 CGGATGTGTT GTGCCTCAGG CTGAGTTCTG AACAGTGCCA TGACCTGGGA CAAGAAGGCC 
     3121 CAGAGGAACT GAGCCATCCC ACTGAATTCC GTCCTGGTGG TGTGACCCCA GAGCGCAGTG 
     3181 CTCAGGCAGA TCTTGGGGTG TTGTTCACGG CTACAGTTGA TCACAGCAGC TCACAAGACC 
     3241 AGTTTCTGTT TTCCAGCTTG CATTCAGAGT CTGACAGGCT GGGGCAGGTG GAGGGAGCGG 
     3301 TCACTTCACA GGACTTGCAA GAAAGCCTAT TCCACATTGC TAGTCCATGC TCAGGTGCTA 
     3361 AAGAAGACAG CGCACACACA GACACTGCCA CGTCTGAGGA AGTGCTGCTG TTCACAGAGC 
     3421 CGAGCAAAGA AGGAGACTCG GAGGCCAAGG TGCGGAGTGT GGGAGCCGGG GGAGCGGAGA 
     3481 AGGAAGTCCA TCAGATCTTC GAGGACCTGG ACCAGCGACT AGCAGTAAGC GCCAGGCTCT 
     3541 TCATCCCAGA GGGCTGCATT CAAAGATGGG CAGCAGAGAT GGTGGTAGCC CTGGACGCCT 
     3601 TGCATAGAGA GGGGATTGTG TGCCGAGATC TGAACCCAAA CAACATCTTA TTGAATGATG 
     3661 GAGGACACAT TCAGCTAACG TATTTTAGCA GGTGGAGTGA GGTTGAAGAT CTCTGTGACA 
     3721 GCGACGCCAT AGCGAGAATG TACTGCGCCC CAGAGGTTGG AGCAGTCACA GAAGAAACTG 
     3781 AAGCCTGTGA TTGGTGGAGC CTGGGTGCTG TCCTCTTCGA ACTTCTCACT GGCAAGACAT 
     3841 TGGTGGAATG CCACCCAGCG GGAATAAATA CTCACACTAC TTTGAACATG CCAGATTGTG 
     3901 TTTCTGAAGA GGCCCGCTCA CTCATCCAAC AGCTCCTGCA GTTCAACCCT ATGGAGCGTC 
     3961 TGGGTGCAGG AGTGGCCGGT GTTGAAGATA TCAAATCTCA CCCGTTCTTC ACCCCTGTGG 
     4021 ATTGGGCAGA ACTGACAAGA ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4081 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4141 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4201 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4261 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4321 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4381 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4441 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4501 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     4561 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     4621 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     4681 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     4741 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     4801 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     4861 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     4921 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     4981 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5041 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5101 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5161 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5221 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5281 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5341 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5401 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5461 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     5521 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     5581 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     5641 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     5701 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     5761 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     5821 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     5881 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     5941 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6001 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6061 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6121 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6181 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6241 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6301 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6361 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6421 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6481 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     6541 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     6601 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     6661 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     6721 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     6781 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     6841 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     6901 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     6961 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7021 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7081 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7141 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7201 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7261 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7321 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7381 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7441 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7501 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     7561 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     7621 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     7681 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     7741 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     7801 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     7861 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//