LOCUS       RR213709                9036 bp    DNA     circular  SYN 
DEFINITION  Pask (Myc-DDK-tagged ORF) - Rat PAS domain containing serine/threonine kinase (Pask), (10 ug)
ACCESSION   NM_001009362
VERSION     NM_001009362.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5193..5284 
                     /label="Myc-DDK"  
     misc_feature    5314..5899 
                     /label="PolyA signal"  
     misc_feature    5371..5390 
                     /label="Restriction Enzyme"  
     misc_feature    6175..6847 
                     /label="ColE1"  
     misc_feature    7277..8071 
                     /label="Kan/Neo"  
     misc_feature    8106..8408 
                     /label="SV40 ori"  
     misc_feature    8494..8971 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5175..5181 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5175 
                     /label="Pask"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGGACAGA GGTCCACCAG TTCTTGCTGA GGACTGGAAG TCTGAGAGTC 
     1081 TTCCTGCCCA AGAGGGCCCA GCTACCCAGG CTTTCGTTGA GCCCAGCAAG CCTCTCTCCA 
     1141 TAGCCCATAG ACACCTGAAC AGGAAGAATG GGCTTTCTAG ACTCTGCCAG AGCCGAATGG 
     1201 CACTCTCCGA GGATAGGTGG AGCTCTTACT GTCTTTCATC ATTGGCTGCC CAAAACATCT 
     1261 GCACAAGCAA ACTGCACTGC GCTGCTACAC CTGACTATGC TGACCCTATA GCTGGCCCAC 
     1321 TGGGCAGTAC ATCGTGCTGC TCCTTGCTAC GTGGCCTAGC TTCAGGGTGC TCTGGACCTC 
     1381 TGCTCTCGAC CCCTGTGTGC AACCCTAACA AGGCTGTCTT TACTGTGGAT GCCAAGACCA 
     1441 CAGAGATTCT GGTTGCCAAT GACAAAGCCT GCAGCCTCCT GGGGTATAGC AGTCATGACC 
     1501 TGATTGGCCA AAAGCTTACA CAGTTCTTCC TCAAGTCTGA TTCCGAGGTT GTGGAAGCCC 
     1561 TCAGCGAGGA GCATGTGGAG GCTGATGGCC ACGCTGCAGT AGTGTTCGGC ACGGTGGTGG 
     1621 ACATTGTCAG CCGAAGTGGG GAGAAGATTC CCGTCTCTGT GTGGATAAAG AGGTTGCAAC 
     1681 AAGATCGTGG CTTGTGCTGT GTAGTGGTTC TGGAGCCTGT GGAGAGGGTC TCAGCCTGGG 
     1741 TCGCCTTCCA GAGTGATGGA ACTATCACGT CATGCGACAG CCTCTTTGCT CATCTTCATG 
     1801 GGTTCACATC TCCAAAGGAT GTGGTTGGGC AGTGTGTCAT AGACTTGATT CCATCTATAC 
     1861 AGCTCCCTCC TCCTGGCCAA CATGTCCCAA AGAGTCTCAA GATTCAAAGG TCTGTTGGGA 
     1921 GAGCCAGAGA CGGGACCACC TTCCCTCTCA GTCTGAAGTT GAAATCTAAG ACCAGTGGAA 
     1981 AGGCTGTAGC TGAAAGTGAG GTGGCCTCAG AACCAGGTTA CCAGGCTTCT GTGTGGGTGT 
     2041 TCTGCACCAT CAGTGGCCTC ATCACTCTCC TGCCTGATGG CACAATTTAT GGCATCAACC 
     2101 ACAGCTTTGC ACTAATGTTG TTTGGTTATG GAAAAACAGA GCTCCTGGGC AAGAACATCA 
     2161 CTTGCCTGAT TCCTGGTTTC TACCACTGCA TGGACCTTGC ATATGATAGT TCTGTCCAGC 
     2221 TGCCAGATCT GGTCAACTGC CTGGACATTG GCAGCAAGAG TGGGCCTGGG GAGATGAGTT 
     2281 CTGATGCCAA GCAGGACTGG GAGCTGGCCA GTGGGGCCCA AGATCCAAGA ATTGATGTGT 
     2341 TGACACGGGG CCACATGCCA TCACAAGATG AGACCCTGAA GCTGCGGGGA GGACAAGTCT 
     2401 CGCCTAGGAC TCAGACTCAG CTGGAAACTG GACGCCACAT ACTTCCTTCT TGTGCATCTC 
     2461 AGCCCTCCCT GGGGGTAGAC AGCAGCCCAG TGGACGGTGA GCGGTCATTA CCCACAGACC 
     2521 AGCGGAACGT TCCAAAGAGA AACCTGCTAG CCCATGCTGA ACAGTTACTA TCTCAGACCC 
     2581 AACCAGACAC CCTGGAAATA TCCCCACCAG TCCTTAAAGA ACTGTCTGAA ATTCAGAAAA 
     2641 ATATCTCAGA AGAAAGCCCA CTAACCCACA GGAAATGGTT ATTGTCCAAG GCCCAGAAAG 
     2701 ACACCACAAA AGGAAGCCTG CCAATCCATG ATGAGAAGTT ACTGCTTGCA AGCCAGCATA 
     2761 TCCATGTCTT GGGAAAGGAA AATCCTTCAG CAGCAGAGAG CTACAGGGAG GGTCTTTTAG 
     2821 CAGAAAGCAG GTCCAAACCA GTGGATGCAA AACCGTTTGC TTCATGTGAA GATTCTGAGC 
     2881 TTCTAGTCCC AGCTAAGGAC AGAAGCAACA GAGCGGACAT GAGTAGTCTG CAACAGGAAG 
     2941 CTCAATTGGA GCTGATGGGT ATCAGTAGTC CCGGTAGTCC TAACCCCTGG GCTGATGCCA 
     3001 CCATGCCTGA GCCCCACACC ACAGGGCAGA TACCAGGAGG GAGCCTGACA TACTGCCCAC 
     3061 AGCATAGAAG CGAGTGGATC TTACAACAGC GAGGCCAGGA CTCGGCTCCC AGCCCCTCTG 
     3121 CAATGGGGTG TGTCTTTGTT GGGACACCCA CTCTGGATGA ACCATGGCCA GGAGTGAGAA 
     3181 ATGATCGAGA AGAGCTGCAG ACTTGCTTGA TTAAGGAACA GTTGTCCAAG TCAAGCTTTG 
     3241 GGGGAGCTCT AGGAATCTCC TGTGTGGAAC TTGTCCCAGC AGAACATCCT CCTTTCACTG 
     3301 CCCCCGTGTC CTTCTGTGAT TGGGGAGGCC GAGACCTACA TGCTAGCCGC TCAGGCAGCT 
     3361 CCTCAGCCTG CTATGCTTTG GCCACTGACC TCCCTGGTGT CTTGGAGGCA GTGGAAGCCC 
     3421 AGGAGGCTGA TGTGAGTTCT TATTCCTGGA ACCTCAGGGA GCTCTTTCTC AATGACCGGA 
     3481 CAGACAGAAC TCCATCACAT TGTTCCTGTA CCACTTCTGA ACTTAGCGAG ACACCATCTC 
     3541 TTTCAGGGGT GGGGTCAGAT TTAGATGTGG GTATTCTGCA CAGGCAGACT TCAGATATTT 
     3601 TAGCTCACCG AGAGATGTTG CTTCTTAGTG GGACCTATTT TGACCTTAGG GAAGGCCATC 
     3661 GGTTCCAGGA GATGGGAGCA GGACATGATG GAGCAGAACT GTCTAACATT TCTTTGGTAT 
     3721 CCTCTGAACA CTATGAAACT AGTGACATAG AAAGCCTAGG TTGTGACCCT CCCATACCAG 
     3781 ATCCTGGTCC TAACAATATG TGCCTATCAG CAGAGAAGCC TAGACTGAGT GCCCAGATCA 
     3841 CTTCCACTCC TGTGGCACCT GGGCCCACCA GCCTGCAGCA GGAGATCCAG GAGGGCATCT 
     3901 TCTCTGGAAG CTGCTACCAC CGAGATGGCT TACGACTGAG TGTACAGTTT GAAGTAAAGC 
     3961 GTGTGCAGCT CCAGGGCTCT GCAACCCTGT TCTGCTGCTG GCTGGTGAAG GACTTCTTAC 
     4021 ACAGTCACCG GGACTCAGCC ACCCGAACCC GCCTGTTCCT TGCCAGCCTG CCAAGCTCTA 
     4081 CCCACTCCAT GCCTGACCTC TCTGGATCCA GCTTTGGAGA GATGCTCAGA GCCAAGCCTT 
     4141 GGTTTGAGGA GTCCCCAACA CCCGCAGAGC TGGAGGGGCT GGCAGCCTGT GAGGGCGAGT 
     4201 ACTCCCATAA GTACAACACC CTGCGCCCAC TCGGCAGTGG CGCCTTTGGC TTTGTGTGGA 
     4261 CTGCAGTGGA GAAGGAAGGC AACAAAGAGG TGGTGGTGAA GTTTATTAAG AAGGAGAAGG 
     4321 TTTTAGAGGA TTGTTGGGTT GAAGATCCCA AACTTGGAAG AGTTCCTTTA GAGATTTCAA 
     4381 TTCTGTCCAA GGTGGAACAT GCCAACATAA TCAAGATTGT GGATGTATTC GAAAACCAAG 
     4441 GGTTCTTTCA GCTAGTGATG GAAAAGCATG GCTCTGGCTT GGACCTCTTT GCTTTCATTG 
     4501 ACCGTCATCC CTGCTTGGAT GAGCCCCTGG CGAGTTACAT CTTCCGACAA CTGGTGTCTG 
     4561 CTGTAAGATA CTTGCGTTCC CAAGGCATCA TCCATAGAGA CATCAAGGAT GAGAACATTG 
     4621 TGATTGCTGA GGACTTCACA ATTAAGCTGA TAGATTTTGG CTCAGCTGCC TACTTAGAGA 
     4681 AGGGCAAACT ATTTTATACC TTTTGTGGAA CAATTGAATA CTGTGCACCT GAAGTTCTCA 
     4741 TTGGAAATCC CTACAGAGGG CCAGAGCTGG AGATGTGGTC TCTCGGGGTC ACGCTGTACA 
     4801 CGCTCATCTT CGAGGAGAAT CCCTTCTGTG AGGTGGAGGA GACCATGGAG GCAGTTATTC 
     4861 ATCCCCCCTT CCTGGTTTCC CAAGAACTTA TGAGTCTTCT GTCTGGGCTG CTGCAGCCTT 
     4921 GCCCTGAGCA GCGGACCACT TTGGAGAAGC TGATTGTGGA CCCCTGGGTG ACACAGCCTG 
     4981 TGAACCTTGA TAACTACACC TGGGAAGAGG TGTGTCGGAC CAGCCAGCCA GAAAGTGGCC 
     5041 TGATGTCAGC TGCAAGTCTG GAGCTTGCGA GTAGGAGTCC AAGTGAAGTG GCTCAGACAG 
     5101 AGGGTCTTTG TAGACCTCCT GCTCCCAAGG AGGCTCCTGG TAACCACTGC TTGCATCTTG 
     5161 AGGATCCCTC GTCACCAGTC AGCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     5221 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     5281 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     5341 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     5401 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     5461 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     5521 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     5581 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     5641 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     5701 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     5761 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     5821 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     5881 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     5941 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     6001 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     6061 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     6121 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     6181 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     6241 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     6301 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     6361 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     6421 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     6481 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     6541 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     6601 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     6661 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     6721 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     6781 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     6841 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     6901 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     6961 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     7021 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     7081 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     7141 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     7201 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     7261 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     7321 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     7381 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     7441 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     7501 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     7561 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     7621 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     7681 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     7741 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     7801 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     7861 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     7921 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     7981 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     8041 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     8101 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     8161 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     8221 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     8281 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     8341 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     8401 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     8461 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     8521 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     8581 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     8641 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     8701 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     8761 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     8821 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     8881 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     8941 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     9001 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//