LOCUS       RR213663                7089 bp    DNA     circular  SYN 
DEFINITION  Gphn (Myc-DDK-tagged ORF) - Rat gephyrin (Gphn), (10 ug)
ACCESSION   NM_022865
VERSION     NM_022865.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3246..3337 
                     /label="Myc-DDK"  
     misc_feature    3367..3952 
                     /label="PolyA signal"  
     misc_feature    3424..3443 
                     /label="Restriction Enzyme"  
     misc_feature    4228..4900 
                     /label="ColE1"  
     misc_feature    5330..6124 
                     /label="Kan/Neo"  
     misc_feature    6159..6461 
                     /label="SV40 ori"  
     misc_feature    6547..7024 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3228..3234 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3228 
                     /label="Gphn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGACCGAG GGAATGATCC TCACAAACCA CGACCATCAA ATCCGTGTCG 
     1081 GAGTCCTCAC AGTGAGTGAT AGCTGCTTCA GGAATCTTGC AGAAGACCGC AGTGGGATAA 
     1141 ATCTAAAAGA TCTCGTCCAG GATCCTTCTT TGTTGGGTGG GACTATATCA GCATACAAGA 
     1201 TAGTACCAGA TGAAATAGAA GAAATCAAGG AAACACTCAT AGACTGGTGT GATGAGAAGG 
     1261 AACTTAATTT GATATTAACA ACTGGCGGAA CAGGGTTTGC ACCACGAGAT GTCACTCCAG 
     1321 AGGCCACAAA AGAAGTAATA GAACGGGAAG CGCCAGGGAT GGCCCTGGCA ATGCTGATGG 
     1381 GATCACTTAA TGTTACACCT CTGGGCATGC TCTCTAGACC AGTGTGTGGA ATAAGAGGGA 
     1441 AAACTCTGAT AATTAACCTG CCTGGTAGCA AGAAAGGATC TCAGGAATGC TTTCAGTTCA 
     1501 TACTGCCAGC TCTACCTCAT GCCATTGACC TTTTACGTGA TGCCATTGTA AAAGTAAAGG 
     1561 AGGTGCATGA TGAACTTGAA GATTTACCTT CCCCACCACC TCCTCTTTCT CCACCTCCTA 
     1621 CAACTAGCCC ACATAAGCAG ACAGAAGACA AAGGAGTTCA GTGTGAAGAG GAGGAAGAAG 
     1681 AAAAGAAAGA CAGTGGTGTA GCTTCGACAG AAGATAGTTC CTCATCACAT ATTACTGCAG 
     1741 CAGCTCTTGC TGCAAAGATT CCAGACTCCA TCATTTCTCG TGGCGTTCAG GTGCTCCCAC 
     1801 GAGACACAGC CTCCCTTAGC ACTACTCCTT CAGAGTCGCC CCGTGCCCAG GCTACATCTC 
     1861 GCCTCTCTAC AGCTTCTTGT CCAACACCAA AAGTCCAGTC CAGGTGCAGC AGCAAGGAGA 
     1921 ACATTCTAAG AGCCAGTCAC AGTGCTGTAG ATATCACCAA GGTGGCTAGA AGACATCGCA 
     1981 TGTCTCCTTT TCCTCTGACG TCTATGGACA AAGCCTTCAT TACAGTTCTG GAGATGACTC 
     2041 CGGTGCTTGG TACAGAAATC ATCAATTACC GAGATGGAAT GGGGCGAGTC CTTGCTCAAG 
     2101 ATGTGTATGC AAAAGACAAC CTACCCCCAT TCCCAGCATC AGTAAAAGAT GGCTATGCTG 
     2161 TTCGAGCTGC TGATGGTCCA GGAGATCGTT TCATCATTGG GGAATCCCAA GCTGGTGAAC 
     2221 AGCCAACTCA GACAGTAATG CCCGGACAAG TGATGCGGGT TACAACAGGT GCTCCAATCC 
     2281 CCTGCGGTGC TGATGCAGTA GTGCAAGTTG AAGATACTGA ACTCATCAGG GAATCAGATG 
     2341 ATGGTACTGA AGAACTTGAA GTGCGAATTC TGGTGCAAGC TCGGCCAGGC CAAGACATCA 
     2401 GACCCATCGG CCACGACATT AAAAGAGGGG AGTGTGTTTT GGCAAAAGGA ACCCATATGG 
     2461 GCCCTTCAGA AATTGGTCTC CTTGCAACTG TAGGTGTCAC AGAAGTTGAA GTTAATAAGT 
     2521 TTCCAGTGGT TGCCGTTATG TCAACAGGGA ATGAGCTACT AAATCCTGAA GATGACCTCT 
     2581 TACCAGGAAA GATTCGGGAC AGCAATCGAT CAACACTTCT AGCAACAATT CAGGAACATG 
     2641 GTTACCCAAC AATCAACTTG GGAATTGTAG GAGACAACCC AGATGACTTA CTCAATGCCT 
     2701 TGAATGAGGG TATCAGCCGT GCTGATGTCA TCATCACGTC AGGAGGTGTA TCCATGGGGG 
     2761 AAAAGGACTA TCTCAAGCAG GTGCTGGACA TCGATCTTCA TGCTCAGATC CATTTTGGCA 
     2821 GGGTTTTTAT GAAGCCAGGC TTGCCAACAA CATTTGCAAC TTTGGATATT GATGGTGTAA 
     2881 GAAAAATTAT TTTTGCACTA CCAGGGAATC CTGTATCAGC TGTGGTCACC TGCAACCTCT 
     2941 TTGTTGTACC CGCACTGAGA AAAATGCAGG GTATCTTGGA TCCTCGGCCA ACCATCATCA 
     3001 AAGCTAGGTT ATCATGTGAC GTAAAGCTGG ACCCTCGCCC AGAATACCAC CGGTGTATAC 
     3061 TGACTTGGCA TCACCAAGAA CCACTGCCCT GGGCCCAGAG TACAGGTAAT CAGATGAGCA 
     3121 GCCGTCTGAT GAGCATGCGC AGTGCCAATG GATTGCTGAT GCTACCTCCA AAGACAGAGC 
     3181 AGTATGTGGA ACTTCACAAA GGCGAGGTGG TGGACGTCAT GGTCATCGGA CGGCTAACGC 
     3241 GTACGCGGCC GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC 
     3301 TGGATTACAA GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC 
     3361 TGAACAGATC CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA 
     3421 GTTGCCACTC CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT 
     3481 GACTAGGTGT CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA 
     3541 GTTGGGAAGA CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG 
     3601 GCACAATCTT GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG 
     3661 CCTCCCGAGT TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT 
     3721 TGGTAGAGAC GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG 
     3781 ATCTACCCAC CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC 
     3841 CCTGTCCTTC TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG 
     3901 CTACCTGGCC ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA 
     3961 TGCGTTGGGT CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT 
     4021 ATCAGCTCAC TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA 
     4081 GAACATGTGA GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC 
     4141 GTTTTTCCAT AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG 
     4201 GTGGCGAAAC CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT 
     4261 GCGCTCTCCT GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG 
     4321 AAGCGTGGCG CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG 
     4381 CTCCAAGCTG GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG 
     4441 TAACTATCGT CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC 
     4501 TGGTAACAGG ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG 
     4561 GCCTAACTAC GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT 
     4621 TACCTTCGGA AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG 
     4681 TGGTTTTTTT GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC 
     4741 TTTGATCTTT TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT 
     4801 GGTCATGAGA TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT 
     4861 TAAATCAATC TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC 
     4921 GTTGGCTGCG AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA 
     4981 AACGCGGGCG TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG 
     5041 GGACCGAACC CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT 
     5101 TATTGCCGTC ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT 
     5161 CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC 
     5221 GGCGTCCCGG AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA 
     5281 ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT 
     5341 CAGAAGAACT CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA 
     5401 CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG 
     5461 GTAGCCAACG CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT 
     5521 CCAGAAAAGC GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG 
     5581 ACGAGATCCT CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG 
     5641 AGCCCCTGAT GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA 
     5701 CGTGCTCGCT CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC 
     5761 GTATGCAGCC GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA 
     5821 GATGACAGGA GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA 
     5881 GTGACAACGT CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC 
     5941 GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC 
     6001 GGGCGCCCCT GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT 
     6061 GCCCAGTCAT AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA 
     6121 TCTTGTTCAA TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC 
     6181 TAGGCCTCCA AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC 
     6241 GGCCTCTGCA TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG 
     6301 CGGAGTTAGG GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG 
     6361 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC 
     6421 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6481 ACACCCTAAC TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT 
     6541 CAATATTATT GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT 
     6601 ATTTAGAAAA ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC 
     6661 GCGCCCTGTA GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT 
     6721 ACACTTGCCA GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG 
     6781 TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT 
     6841 GCTTTACGGC ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA 
     6901 TCGCCCTGAT AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA 
     6961 CTCTTGTTCC AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA 
     7021 GGGATTTTGC CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC 
     7081 GCGAATTTT
//