LOCUS       RR213661                9171 bp    DNA     circular  SYN 
DEFINITION  Ssh2 (Myc-DDK-tagged ORF) - Rat slingshot homolog 2 (Drosophila) (Ssh2), (10 ug)
ACCESSION   NM_001107024
VERSION     NM_001107024.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5328..5419 
                     /label="Myc-DDK"  
     misc_feature    5449..6034 
                     /label="PolyA signal"  
     misc_feature    5506..5525 
                     /label="Restriction Enzyme"  
     misc_feature    6310..6982 
                     /label="ColE1"  
     misc_feature    7412..8206 
                     /label="Kan/Neo"  
     misc_feature    8241..8543 
                     /label="SV40 ori"  
     misc_feature    8629..9106 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5310..5316 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5310 
                     /label="Ssh2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACCCTTAGC ACTTTGGCCG ACGAAAGGAA AGCGCCCCCT GCCAGCACCT 
     1081 GCAGCCTCGG TGGCCCCGAC ATGATCCCCT ACTTCTCTGC CAATGCGGTT ATCTCGCAGA 
     1141 ACGCCATCAA CCAGCTCATC AGCGAGAGCT TTTTAACTGT CAAAGGTGCT GCCCTTTTTC 
     1201 TACCACGAGG GAATGGCTCG TCTACACCAC GAATCAGCCA CAGACGCAAC AAGCATGCAG 
     1261 GAGATCTCCA ACAGCATCTT CAAGCAATGT TCATATTACT CCGCCCAGAA GACAACATAA 
     1321 GGCTGGCTGT AAGACTGGAA AGTACTTACC AAAATCGAAC ACGCTATATG GTAGTGGTTT 
     1381 CAACTAACGG TAGACAAGAC ACTGAAGAAA GCATTGTCCT GGGAATGGAT TTTTCTTCTA 
     1441 ATGACAGTAG CACTTGTACC ATGGGCTTGG TCTTGCCTCT CTGGAGCGAC ACCCTAATTC 
     1501 ATTTGGATGG TGATGGTGGG TTCAGCGTGT CAACAGATAA CAGAGTTCAC ATATTCAAGC 
     1561 CTGTATCTGT GCAAGCAATG TGGTCTGCAC TACAGAGTTT GCACAAAGCT TGTGAAGTGG 
     1621 CCAGGATGCA TAACTACTAT CCAGGCAGCC TTTTTCTCAC TTGGGTGAGT TACTATGAGA 
     1681 GCCACATAAA CTCAGACCAG TCCTCAGTCA ACGAATGGAA TGCTATGCAG GATGTGCAGT 
     1741 CTCATCGGCC TGACTCTCCG GCCCTCTTCA CAGACATACC AACTGAACGT GAGCGAACAG 
     1801 AAAGGCTTAT TAAGACTAAG TTAAGGGAGA TTATGATGCA AAAGGATTTG GAGAATATCA 
     1861 CATCCAAAGA GATACGCACA GAACTGGAAA TGCAGATGGT GTGCAACTTG CGGGAGTTTA 
     1921 AGGAGTTCAT AGATAACGAG ATGATCGTGA TCCTGGGTCA GATGGACAGT CCGACCCAGA 
     1981 TATTTGAGCA CGTGTTCCTG GGCTCAGAAT GGAATGCGTC AAACTTAGAG GACTTGCAGA 
     2041 ACCGAGGGGT GCGGTATATT TTGAATGTCA CTCGAGAGAT AGATAACTTC TTCCCTGGAG 
     2101 TCTTTGAGTA TCATAACATT CGAGTATATG ATGAAGAGGC AACAGATCTC CTGGCTTACT 
     2161 GGAATGACAC TTACAAATTC ATCTCTAAAG CAAAGAAACA TGGATCTAAA TGTCTTGTGC 
     2221 ACTGCAAAAT GGGGGTGAGT CGCTCAGCCT CCACTGTGAT TGCCTATGCG ATGAAGGAGT 
     2281 ATGGATGGAA TCTGGATCGC GCCTATGAGT ACGTGAAGGA ACGACGAACA GTGACCAAGC 
     2341 CCAACCCCAG CTTCATGAGA CAACTAGAAG AATACCAAGG GATCTTGCTG GCCAGCAAAC 
     2401 AGCGGCATAA CAAACTCTGG AGATCTCATT CAGATAGTGA CCTCTCAGAC CACCACGAAC 
     2461 CCATCTGTAA ACCTGGGCTC GAGCTCAACA AGAAGGAGAT GACCACTTCA GCAGACCAGA 
     2521 TCACTGAGGT GAAGACTGTG GAGAGCCTTG CAGCCATGCC TCCCGTCTTC ATGGAACATG 
     2581 TGGTCCCACA AGATGTAAAT CAGAAAGGAC TGCGCACCAA AGAAAGAGTC ATCTGCTTGG 
     2641 AGTTTTCTTC ACAAGAGTTT CATGCCGGCC AGATTGAAGA TGAATTAAAC TTAAATGACA 
     2701 TTAATGGATG CTCGTCAGGG TGTTGTCTCA GCGAATCAAA ATTCCCTCTT GACAACTGCC 
     2761 ATGCATCTAA AGCCTTACTC CAACCTGGAC AGGCCCCGGA CATTGCCAAC AAGTTCCCGG 
     2821 ACTTAGCGGT GGAAGACTTG GAGACAGATG CACTGAAAGC AGACGTGAGG GTCCACTTAC 
     2881 TGCCAATGGA AGAGTTGACA TCGAGACTGA AAGACCTCCC TATGTCACCT GATCTGGAGT 
     2941 CACCAAGCCC CCAAGCCGGT TGCCAAGCTG CCATCTCTGA TTTTAGTACA GATCGCATTG 
     3001 ATTTTTTTAG TGCCCTAGAG AAATTTGTAG AGCTTTCTCA GGAAACCCGG TCTCGGTCTT 
     3061 TTTCTCACTC AAGGATAGAA GAATTAGGAG GAGGAAGGAG CGAGGGCTGT CGCCTGTCGG 
     3121 TGATGGAAAT GGCACCTTCA GAAATGGTGA CTGATGACCA GAGAAGCAGC TCTTTGAGTG 
     3181 ATACTCCCCA CGCATCTGAA GAGTCTTCTG TGGATGAGGA CCAGCCGAAG GCAGTCTCAG 
     3241 AACTTGTCAG CCCAGACATC ATCATGCAAT CTCACTCAGA AAATGCAATT TCAGTCAAAG 
     3301 AAATTGTCAC TGAAATTGAG TCCATTAGTC AAGGAGTTGG GCAGGTTCAG TTAAAAGGAG 
     3361 ACATCCTCTC TAACCCATGC CATACACCAA AGAAGAACAT CATTCATGAG CTGCCCCTGG 
     3421 AGAGAGTACA AGGCCCTGAG AACAAACCTG GACATTTGGA GCAGGATGAG AGTTTCTGTA 
     3481 GTGTCCAACT AGAACTAGCC AGAGACTCGG GGAAGTGTGC CCCAGAGGAG GGCTGTCTGG 
     3541 CCACACTCTC GTCCACAGGA GACTTGGAAG AAGAGGAACC AGTTGAGGGT GAACATGACT 
     3601 GGGGCCCAGG GACACACCCT GGTGCCAAGT GGTGCCCTGG GTCTGTGAGG CGAGCCACCC 
     3661 TGGAGTTTGA AGAGCGCTTG CGACAAGAGC AGGAGAACCA TGGGACAGCC TCTCCAGGTC 
     3721 CCACGTTATC CAATCGCAAA AACTCTAAGA ATGACTCTTC TGTGGCAGAC CTAATGCCAA 
     3781 AATGGAAAAG CGATGAAACC GCCTCAGAGC ATTCACTTTC CCCCAAGGAA GCCGAGCCCG 
     3841 GCAAGGGTAA AGGGAAGTGC AGTGGCTCTG AGGCCGGACC CCTGTCCCAC TGTGAGCATA 
     3901 ATCCCGCTGC TGCAGCCCCC CAGCTGCTGG AGCATCACCC CTCGCCAGCT CCTCAGGACT 
     3961 GCCTGGGGTC AGAGAGCAGA AGTGAGAAGC AGGAAGAAGA CCTGAGGAAG CAGAGGACTG 
     4021 TGGTTTCAAA CCAGGAATGT GAGACACAGG CCGCCCTTCT TCCCCTCCCC AAGAAAATAG 
     4081 AAATCATTGA GTATACACCA ACAGTTACAT CACTTGATCA CACTGAGCCA GGAGGTGAGA 
     4141 CAACACCCAG CAAAGAGAGT GAGAAGCAAG AGCTGAGGAA GGTGAAGATG GAGAGGTCTA 
     4201 TCACTATGTT TTGCACTCTG GATGAAAATC TGAACAGGAC TCTGGACCCC AGTCAGGTTT 
     4261 CTCTGCGTCC CCAAGTGCTA CCTCTGCCTC ATTCTTCCTC TGAGTATGAC AGGCTCACTG 
     4321 ACCCAACCCC TATGTTAAGT AGCCCTCAAG ACAAAGGGGA CAGCCCATCC ACACCCTTCA 
     4381 AGACACCAGC GCGTTTGGTC GGTTGCAGTA CCCAAGGAGC ATCTGTCAGC TTGGATTGTT 
     4441 CGCATCGCCA TTCTGTGCTT CACCTGGAAG GCTGCACAGA GCAAAGCAGT ACCACAGACA 
     4501 GCAGACTGTC TTCAGAGCAC ATGAACTGGG AGGACAGCCA GGGGGACTTC CTTTCCAGTA 
     4561 GTACTGGAAT GGCACACACG TCCTCTCCAT TAACAAATGA AGACCTAAGT TTAATTAACA 
     4621 AACTTGGTGA CAGTGTTGGC GTGTTACCGA AAAAACTGGA TCCATCACCG GAAGCCTGTC 
     4681 GAATCCCACA TAGCTCTAGT AGTGAAAATA TAAGAGATCT CAGCCACGGC CCTGGTGTGG 
     4741 TGAAGGAGCA TGCTAAAGAA ATTGAGCCTC GAGTGATCTT CCAGGCGGCG TTCTCCAAAA 
     4801 CATCACAAAT GAGGCGCTCA GCTTCGCTGG CCAAGTTGGG TTACCTGGAC CTTTGTAAAG 
     4861 ACTACTTACC GGATAGAGAG CTTGGCTCCT CAGAATCCCC TCATCTCAAA TTGCTTCAGC 
     4921 CCTTCCTCAG AACAGACTCA GGCATGCATG CCTTGATGGT CCATGAGCCC TCAGAAAGCC 
     4981 CAGGTGCCCA CCCAAACCCA CAGCCCACCA AGTATTTTGT AGAGCAACTC AAAACAACAG 
     5041 AGTGTGTTGT GCAGAGCAAG CCAGTGGAGA GGCCCCATGT GCAGTATGCC AAAGAGTTTG 
     5101 GTTTCAGTCA GCAGTGTTTG CTCCCCAAGG CAAGACCAGA ATTGACTAGT TCTGAAGGAG 
     5161 GCCTTCCTTT GCTACAGACA CAGGGTCTGC AGTATCCAGG CCCCTCTCCA GGGCTGGCTG 
     5221 TGGCTCCCCG TCAGCAGCAT GGCAGGACTC ACCCGCTTAG GAGACTAAAA AGAGCAAATG 
     5281 ATAAAAAACG GACAACCAAC CCCTTCTATA ACACCATGAC GCGTACGCGG CCGCTCGAGC 
     5341 AGAAACTCAT CTCAGAAGAG GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG 
     5401 ACGATAAGGT TTAAACGGCC GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG 
     5461 CATCCCTGTG ACCCCTCCCC AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC 
     5521 ACCAGCCTTG TCCTAATAAA ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT 
     5581 AATATTATGG GGTGGAGGGG GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT 
     5641 AGGGCCTGCG GGGTCTATTG GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT 
     5701 GCAATCTCCG CCTCCTGGGT TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA 
     5761 TTCCAGGCAT GCATGACCAG GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC 
     5821 ACCATATTGG CCAGGCTGGT CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT 
     5881 CCCAAATTGC TGGGATTACA GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA 
     5941 AAATAACTAT ACCAGCAGGA GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA 
     6001 CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG 
     6061 TAGATGCCTG TTGAATTGGG TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC 
     6121 GGTAATACGG TTATCCACAG AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG 
     6181 CCAGCAAAAG GCCAGGAACC GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG 
     6241 CCCCCCTGAC GAGCATCACA AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG 
     6301 ACTATAAAGA TACCAGGCGT TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC 
     6361 CCTGCCGCTT ACCGGATACC TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA 
     6421 TAGCTCACGC TGTAGGTATC TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT 
     6481 GCACGAACCC CCCGTTCAGC CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC 
     6541 CAACCCGGTA AGACACGACT TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG 
     6601 AGCGAGGTAT GTAGGCGGTG CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC 
     6661 TAGAAGAACA GTATTTGGTA TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT 
     6721 TGGTAGCTCT TGATCCGGCA AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA 
     6781 GCAGCAGATT ACGCGCAGAA AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG 
     6841 GTCTGACGCT CAGTGGAACG AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA 
     6901 AAGGATCTTC ACCTAGATCC TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT 
     6961 ATATGAGTAA CCTGAGGCTA TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG 
     7021 GCCTTCACCC GAACTTGGGG GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC 
     7081 CCAATGGGGT CTCGGTGGGG TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT 
     7141 ATGAACAAAC GACCCAACAC CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG 
     7201 GGTTCCTTCC GGTATTGTCT CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA 
     7261 GAACTCCAGC ATGAGATCCC CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT 
     7321 TCCGAAGCCC AACCTTTCAT AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT 
     7381 GGGCGTCGCT TGGTCGGTCA TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA 
     7441 AGGCGATAGA AGGCGATGCG CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG 
     7501 CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC 
     7561 TGATAGCGAT CCGCCACACC CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT 
     7621 TCCACCATGA TATTCGGCAA GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG 
     7681 GGCATGCTCG CCTTGAGCCT GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG 
     7741 TCCAGATCAT CCTGATCGAC AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA 
     7801 TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT 
     7861 GCATCAGCCA TGATGGATAC TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC 
     7921 CCCGGCACTT CGCCCAATAG CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA 
     7981 GCTGCGCAAG GAACGCCCGT CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT 
     8041 TCATTCAGGG CACCGGACAG GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC 
     8101 AGCCGGAACA CGGCGGCATC AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT 
     8161 AGCCTCTCCA CCCAAGCGGC CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA 
     8221 AACGATCCTC ATCCTGTCTC TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC 
     8281 TCCTCACTAC TTCTGGAATA GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA 
     8341 AAAAATTAGT CAGCCATGGG GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT 
     8401 GGGCGGAGTT AGGGGCGGGA CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     8461 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT 
     8521 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC 
     8581 ATTCCACAGC TGGTTCTTTC CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT 
     8641 TATCAGGGTT ATTGTCTCAT GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA 
     8701 ATAGGGGTTC CGCGCACATT TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA 
     8761 TTAAGCGCGG CGGGTGTGGT GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA 
     8821 GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT 
     8881 CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC 
     8941 CCCAAAAAAC TTGATTAGGG TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT 
     9001 TTTCGCCCTT TGACGTTGGA GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA 
     9061 ACAACACTCA ACCCTATCTC GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG 
     9121 GCCTATTGGT TAAAAAATGA GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//