LOCUS       RR213612                8532 bp    DNA     circular  SYN 
DEFINITION  Smc3 (Myc-DDK-tagged ORF) - Rat structural maintenance of chromosomes 3 (Smc3), (10 ug)
ACCESSION   NM_031583
VERSION     NM_031583.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4689..4780 
                     /label="Myc-DDK"  
     misc_feature    4810..5395 
                     /label="PolyA signal"  
     misc_feature    4867..4886 
                     /label="Restriction Enzyme"  
     misc_feature    5671..6343 
                     /label="ColE1"  
     misc_feature    6773..7567 
                     /label="Kan/Neo"  
     misc_feature    7602..7904 
                     /label="SV40 ori"  
     misc_feature    7990..8467 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4671..4677 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4671 
                     /label="Smc3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACATCAAG CAGGTGATTA TCCAGGGCTT CCGAAGTTAC CGAGATCAAA 
     1081 CAATTGTAGA TCCCTTCAGT TCAAAACATA ATGTTATTGT GGGCAGAAAT GGATCTGGAA 
     1141 AAAGCAACTT TTTTTATGCA ATCCAGTTTG TTCTCAGTGA TGAATTTAGT CATCTTCGTC 
     1201 CAGAGCAGCG ATTGGCTTTG TTGCACGAGG GTACAGGTCC TCGTGTTATT TCTGCTTTTG 
     1261 TGGAAATCAT TTTTGACAAT TCAGACAACC GGTTACCAAT TGATAAAGAG GAAGTTTCGC 
     1321 TTCGAAGAGT TATTGGTGCC AAAAAAGATC AGTATTTCCT AGATAAGAAG ATGGTCACGA 
     1381 AAAATGATGT GATGAATCTC CTTGAAAGTG CTGGGTTTTC GAGAAGTAAT CCTTATTACA 
     1441 TTGTTAAACA AGGAAAGATC AACCAAATGG CAACAGCACC GGATTCTCAG AGATTAAAGC 
     1501 TGTTGAGAGA AGTAGCTGGC ACTCGAGTGT ATGACGAGCG CAAAGAGGAA AGCATCTCCC 
     1561 TGATGAAAGA AACAGAGGGC AAACGGGAAA AGATCAATGA GTTGTTAAAA TACATTGAAG 
     1621 AGCGATTACA TACTCTAGAG GAGGAGAAGG AGGAACTGGC CCAGTATCAG AAGTGGGATA 
     1681 AAATGAGGCG TGCCCTGGAG TACACCATCT ACAACCAGGA GCTCAACGAG ACTCGCGCCA 
     1741 AGCTTGATGA GCTTTCTGCT AAGCGAGAAA CAAGCGGAGA GAAATCCAGA CAATTAAGAG 
     1801 ATGCCCAGCA GGATGCAAGA GATAAAATGG AGGATATTGA GCGCCAAGTT AGAGAACTGA 
     1861 AAACAAAAAT TTCAGCCATG AAAGAAGAAA AAGAACAGCT CAGTGCGGAG AGACAAGAGC 
     1921 AGATTAAGCA AAGGACCAAG CTGGAGCTTA AAGCCAAGGA CTTACAGGAT GAGCTGGCAG 
     1981 GGAACAGTGA GCAGCGGAAA CGTTTATTAA AAGAAAGACA GAAGCTGCTT GAAAAAATAG 
     2041 AAGAAAAGCA GAAAGAACTG GCAGAAACAG AACCGAAATT CAACAGCGTA AAGGAAAAAG 
     2101 AAGAGCGAGG AATTGCTAGA TTGGCTCAAG CTACACAGGA AAGAACTGAT CTTTATGCAA 
     2161 AGCAGGGTCG AGGAAGCCAG TTTACATCAA AAGAAGAAAG AGATAAGTGG ATTAAGAAGG 
     2221 AACTGAAGTC TTTAGATCAG GCTATTAATG ATAAGAAAAG ACAGATTGCT GCTATACACA 
     2281 AGGATTTGGA GGATACCGAG GCCAATAAAG AGAAAAATCT GGAGCAATAT AATAAACTGG 
     2341 ATCAGGATCT CAATGAAGTC AAAGCTCGAG TTGAAGAACT GGACAGAAAA TATTATGAAG 
     2401 TGAAAAATAA GAAAGATGAA CTACAAAGTG AAAGAAATTA CTTGTGGAGA GAGGAGAATG 
     2461 CAGAACAGCA AGCACTTGCC GCTAAGAGAG AAGACCTTGA GAAGAAGCAG CAGCTTCTTA 
     2521 GAGCGGCAAC AGGAAAGGCC ATTTTAAATG GAATCGACAG CATTAACAAA GTGTTAGACC 
     2581 ATTTCCGGCG GAAGGGTATA AACCAGCATG TGCAAAATGG CTACCATGGC ATCGTGATGA 
     2641 ATAACTTTGA GTGCGAACCA GCTTTCTATA CTTGTGTGGA AGTCACTGCC GGTAACAGGT 
     2701 TATTCTATCA CATTGTCGAT TCAGATGAAG TCAGCACGAA GATCTTAATG GAGTTCAATA 
     2761 AAATGAATCT TCCTGGAGAG GTGACTTTCC TGCCTCTTAA CAAGTTAGAT GTGAGGGACA 
     2821 CTGCCTATCC TGAAACTAAC GATGCTATTC CTATGATCAG TAAGCTGAGG TACAATCCCA 
     2881 GATTCGACAA AGCTTTCAAA CATGTGTTTG GAAAGACACT CATCTGTCGG AGCATGGAGG 
     2941 TTTCCACTCA GCTAGCTCGT GCCTTCACTA TGGACTGCAT TACTCTGGAA GGTGATCAAG 
     3001 TCAGCCATCG AGGTGCTCTG ACTGGAGGCT ATTACGACAC AAGAAAGTCT CGCCTTGAGT 
     3061 TACAGAAGGA TGTTAGGAAA GCAGAGGAGG AGCTGGGTGA GCTCGAAGCT AAGCTCAATG 
     3121 AAAACCTGCG CAGAAACATT GAAAGGATTA ATAATGAAAT TGATCAATTG ATGAACCAAA 
     3181 TGCAGCAGAT AGAGACCCAA CAGAGAAAAT TTAAAGCATC CAGAGATAGC ATCTTATCAG 
     3241 AGATGAAGAT GCTAAAAGAG AAGAGACAGC AGTCAGAAAA GACCTTCATG CCAAAGCAAC 
     3301 GCAGTTTGCA GAGTCTGGAG GCCAGTCTGC ACGCTATGGA GTCGACCAGA GAGTCGCTGA 
     3361 AGGCAGAGCT AGGGACAGAT TTGCTCTCTC AGCTCAGCCT GGAAGACCAG AAAAGAGTCG 
     3421 ACGCACTGAA TGACGAGATC CGGCAGCTTC AGCAGGAAAA CAGACAGCTG CTGAATGAAA 
     3481 GAATTAAACT AGAAGGCATT ATTACTCGAG TAGAGACTTA CCTCAATGAG AATCTGAGGA 
     3541 AACGTCTGGA CCAAGTAGAG CAGGAACTTA ATGAACTGAG AGAGACAGAA GGTGGCACTG 
     3601 TTCTTACTGC CACCACGTCA GAACTTGAAG CTATTAATAA AAGAGTGAAA GATACTATGG 
     3661 CAAGATCAGA AGATTTGGAT AATTCCATTG ACAAAACAGA AGCTGGAATT AAAGAGCTCC 
     3721 AGAAAAGCAT GGAGCGCTGG AAGAACATGG AGAAGGAGCA CATGGATGCC ATAAATCACG 
     3781 ACACTAAAGA GCTGGAGAAG ATGACCAACC GACAGGGCAT GCTGTTGAAG AAGAAAGAGG 
     3841 AGTGCATGAA GAAGATCCGA GAGCTGGGGT CCCTTCCCCA GGAAGCATTT GAAAAGTACC 
     3901 AGACACTGAG CCTGAAGCAG TTGTTTCGGA AACTTGAGCA GTGCAACACA GAGTTGAAGA 
     3961 AGTACAGCCA TGTCAACAAG AAGGCTCTGG ACCAGTTTGT GAATTTCTCT GAGCAGAAGG 
     4021 AGAAACTGAT AAAGCGACAG GAAGAATTGG ACAGGGGCTA CAAGTCAATC ATGGAGTTGA 
     4081 TGAATGTACT CGAACTTAGA AAATATGAAG CTATCCAGTT AACCTTCAAA CAGGTATCTA 
     4141 AGAACTTTAG TGAAGTTTTC CAGAAGTTGG TACCTGGGGG AAAAGCGACC TTGGTGATGA 
     4201 AGAAAGGAGA CGTGGAGGGC AGCCAGTCTC AGGACGAAGG AGAGGGGAGC GGCGAGAGCG 
     4261 AGAGGGGCTC TGGGTCACAA AGCAGCGTTC CATCAGTTGA CCAGTTCACA GGAGTCGGGA 
     4321 TCAGGGTATC ATTTACAGGA AAGCAAGGCG AGATGAGAGA AATGCAGCAG CTTTCAGGAG 
     4381 GGCAGAAGTC TCTGGTAGCC CTTGCTCTCA TTTTTGCCAT TCAGAAGTGT GACCCTGCTC 
     4441 CTTTCTACCT GTTTGATGAG ATCGACCAAG CTTTGGATGC TCAGCACAGA AAAGCTGTGT 
     4501 CAGATATGAT TATGGAACTA GCTGTACACG CCCAGTTTAT CACTACCACT TTTAGGCCTG 
     4561 AACTGCTTGA GTCTGCTGAT AAATTCTACG GTGTAAAGTT CAGAAATAAG GTTAGTCACA 
     4621 TCGACGTGAT CACAGCAGAG ATGGCCAAAG ACTTTGTAGA AGACGATACC ACGCATGGTA 
     4681 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     4741 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     4801 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     4861 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     4921 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     4981 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5041 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5101 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5161 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5221 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5281 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5341 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     5401 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     5461 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     5521 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     5581 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     5641 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     5701 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     5761 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     5821 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     5881 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     5941 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6001 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6061 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6121 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6181 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6241 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6301 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6361 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     6421 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     6481 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     6541 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     6601 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     6661 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     6721 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     6781 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     6841 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     6901 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     6961 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7021 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7081 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7141 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7201 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7261 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7321 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7381 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     7441 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     7501 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     7561 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     7621 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     7681 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     7741 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     7801 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     7861 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7921 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     7981 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8041 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8101 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8161 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8221 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8281 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8341 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     8401 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     8461 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     8521 AACGCGAATT TT
//