LOCUS       RR213548                9333 bp    DNA     circular  SYN 
DEFINITION  Aqr (Myc-DDK-tagged ORF) - Rat aquarius homolog (mouse) (Aqr), (10 ug)
ACCESSION   NM_001100987
VERSION     NM_001100987.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5490..5581 
                     /label="Myc-DDK"  
     misc_feature    5611..6196 
                     /label="PolyA signal"  
     misc_feature    5668..5687 
                     /label="Restriction Enzyme"  
     misc_feature    6472..7144 
                     /label="ColE1"  
     misc_feature    7574..8368 
                     /label="Kan/Neo"  
     misc_feature    8403..8705 
                     /label="SV40 ori"  
     misc_feature    8791..9268 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5472..5478 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5472 
                     /label="Aqr"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCTCCT GCGCAGCCCA AGAAAATCGT GGCCCCCACC GTGTCCCAGA 
     1081 TCAACGCAGA GTTCGTCACC CAGCTGGCAT GTAAATACTG GGCTCCTCAT ATCAAGAAGA 
     1141 AATCACCTTT TGATATAAAA GTGATAGAAG AAATATATGA AAAAGAGATC GTCAAATCAC 
     1201 GGTTTGCCAT CAGAAAAATA ATGCTGCTGG AATTTAGCCA ATATCTTGAA AATTATCTTT 
     1261 GGATGAATTA TTCGCCAGAA GTTTCCAGCA AAGCCTATTT AATGTCCATC TGCTGTATGG 
     1321 TGAATGAGAA GTTCAGAGAA AATGTCCCAG CCTGGGAGAC CTTTAAGAAG AAGCCAGACC 
     1381 ACTTCCCCTT CTTCTTTAAA CGCATCTTGA AAGCAGCACT AGCTGAGACT GATGGCGAGT 
     1441 TCTCCCTCCA CGAGCAGACC GTGCTCCTGC TTTTCCTGGA TCATTGCTTC AATAGCTTGG 
     1501 AAGTAGACTT GATAAGGAGT CAAGTACAGC AGCTTATCTC CCTTCCTATG TGGATGGGCT 
     1561 TGCAGCCGGC ACGATTGGAA TTAGAATTAA AAAAGACACC AAAGCTAAGG AAATTCTGGA 
     1621 ACTTGATTAA AAAGAATGAT GAAAAGATGG ACCCAGAAGC AAGAGAGCAG GCCTACCAAG 
     1681 AAAGGAGGTT TCTTTCCCAG CTCATCCAGA AGTTCATCTC CGTGTTGAAG TCCATCCCAC 
     1741 TTTCTGAGCC TGTCACTATG GACAAAGTTC ATTACTGTGA AAGATTCATC GAACTTATGA 
     1801 TTGATCTAGA GGCTCTGCTC CCCACAAGGC GCTGGTTCAA CACCATCCTG GATGACTCCC 
     1861 ACCTCTTGGT GCACTGTTAC CTTTCCAGTC TTGTCCACAG GGAAGAGGAT GGCCATCTGT 
     1921 TTTCCCAGCT ATTAGACATG CTGAAGTTCT ATACGGGCTT TGAAATTAAC GACCAGACTG 
     1981 GAAATGCCCT GACAGAGAAC GAGATGACCA CGATTCACTA TGACAGAATC ACATCTCTGC 
     2041 AGAGAGCTGC GTTCGCGCAT TTCCCTGAGC TCTATGACTT TGCCCTCTCC AATGTGGCAG 
     2101 AAGTAGATGC CCGGGATTCC TTGGTCAAGT TTTTTGGGCC TCTCAGTTCA AACACACTCC 
     2161 ACCAGGTGGC ATCATACCTC TGCCTCTTGC CAACGCTCCC TAAAAATGAA GATACAACTT 
     2221 TTGATAAAGA GTTTCTTCTA GAATTGCTGG TGTCTCGCCA TGAACGCCGC ATCTCTCAGA 
     2281 TTCAGCAGTT GAATCAGATG CCTTTGTATC CTACTGAGAA AATTATCTGG GATGAAAACA 
     2341 TCGTCCCTAC CGAGTACTAT TCTGGGGAAG GCTGTCTTGC TCTCCCCAAG TTGAATTTGC 
     2401 AGTTTTTAAC TCTACATGAC TATCTCCTGA GGAACTTTAA TCTCTTCCGC TTAGAATCAA 
     2461 CTTATGAAAT TAGACAAGAC ATTGAGGACA GCGTCAGCAG AATGAAGCCA TGGCAATCTG 
     2521 AGTATGGCGG TGTCGTGTTT GGTGGTTGGG CACGAATGGC TCAGCCCATT GTGGCTTTCA 
     2581 CTGTAGTCGA GGTTGCCAAA CCCAACATTG GTGAAAACTG GCCAACCCGA GTTCGTGCAG 
     2641 ATGTCACCAT CAATCTGAAT GTCAGAGACC ACATCAAGGA TGAGTGGGAA GGTCTTCGCA 
     2701 AACACGATGT ATGCTTTTTA ATTACTGTGC GTCCCACAAA ACCTTACGGC ACTAAGTTTG 
     2761 ACAGGAGGAG GCCTTTTATT GAGCAAGTTG GTCTGGTGTA CGTCAGAGGC TGTGAGATCC 
     2821 AGGGCATGCT GGACGACAAA GGGCGAGTCA TCGAAGACGG ACCTGAACCC AGACCCAATC 
     2881 TTAGAGGAGA GTCAAGGACA TTTCGAGTAT TTTTGGATCC AAACCAGTAT CAACAGGATA 
     2941 TGACCAATAC TATACAAAAT GGAGCCGAAG ATGTGTACGA CACTTTCAAT GTCATAATGA 
     3001 GGAGGAAACC AAAGGAGAAT AACTTCAAGG CCGTGTTGGA GACTATTCGA AATCTGATGA 
     3061 ATACCGACTG CGTGGTCCCA GACTGGCTGC ATGACATCAT TTTAGGTTAT GGGGACCCAA 
     3121 GTAGTGCCCA TTATTCCAAA ATGCCCAATC AGATTGCTAC GTTGGATTTC AATGACACTT 
     3181 TTCTCTCCAT TGAACATTTG AAAGCCAGTT TTCCTGGTCA TAATGTCAAA GTGACCGTGA 
     3241 ATGACCCAGC TCTACAAATA CCCCCTTTCA GAATAACTTT CCCAGTAAGA AGTGGAAAGG 
     3301 GGAAGAAAAG AAAAGATGCA GATGGGGAAG AAGACGACAC GGAAGAAGCA AAGACCTTAA 
     3361 TTGTGGAACC TCACGTTATT CCTAATAGAG GTCCTTATCC TTACAACCAG CCCAAACGTA 
     3421 ATACAATCCA GTTCACTCAT ACACAGATAG AGGCCATCCG GGCCGGAATG CAGCCTGGAC 
     3481 TGACTATGGT TGTGGGACCA CCTGGTACAG GAAAAACAGA TGTTGCAGTC CAGATCATTT 
     3541 CCAACATCTA CCACAATTTT CCAGAACAGA GGACTTTAAT TGTTACTCAT TCCAATCAGG 
     3601 CTCTGAACCA GTTGTTTGAG AAAATCATGG CTTTAGACAT TGATGAGCGC CATCTCCTGC 
     3661 GTCTGGGTCA TGGAGAGGAA GAGCTAGAGA CGGAGAAAGA CTTTAGCAGG TATGGAAGAG 
     3721 TTAATTATGT CCTGGCTCGA AGAATAGAAC TTTTAGAAGA AGTCAAACGA TTACAAAAGA 
     3781 GTCTGGGGGT TCCGGGAGAT GCTTCGTATA CCTGTGAAAC TGCAGGCTAT TTCTTCTTAT 
     3841 ATCAGGTAAT GTCGCGTTGG GAAGAATACA TGAGCAGAGT GAAGAACACA GGGAGCGCCA 
     3901 GCCCAGATGC TGCGGAGGTC GCCACGTTCT TCCCCTTCCA TGAGTATTTT GCCAATGCTC 
     3961 CTCAGCCCAT CTTTAAAGGC AGATCTTATG AAGAAGACAT GGAAATCGCA GAAGGGTGCT 
     4021 TCAGGCATAT TAAGAAAATC TTCACTCAGC TTGAGGAATT CAGAGCTTCA GAACTGCTTC 
     4081 GCAGTGGACT GGACAGGTCT AAATACCTCT TGGTGAAAGA AGCCAAAATC ATTGCTATGA 
     4141 CGTGTACTCA TGCTGCCTTG AAACGACATG ACTTGGTCAA GTTGGGTTTC AAGTATGACA 
     4201 ACATTTTAAT GGAAGAGGCT GCTCAGATTC TGGAGATAGA AACCTTTATC CCACTACTTC 
     4261 TGCAGAATCC TCAAGACGGA TTCAGCCGAT TGAAACGATG GATTATGATT GGTGATCACC 
     4321 ACCAATTGCC TCCAGTCATT AAGAACATGG CCTTTCAGAA GTATTCCAAT ATGGAGCAGT 
     4381 CTCTTTTTAC TCGCTTTGTC CGGGTTGGAG TTCCTACTGT GGACCTTGAT GCTCAAGGGA 
     4441 GAGCCCGGGC CAGCTTGTGT AACCTCTACA ACTGGCGTTA CAAAAACCTA GGGAACTTGC 
     4501 CACACGTGCA GCTCCTGCCA GAGTTCAGCA CAGCCAACGC AGGCCTGCTG TACGACTTCC 
     4561 AGCTCATCAA TGTGGAAGAC TTCCAGGGAG TGGGAGAGTC GGAGCCTAAC CCTTACTTCT 
     4621 ACCAGAACCT CGGAGAGGCA GAGTACGTAG TAGCGCTCTT CATGTACATG TGCTTGCTTG 
     4681 GCTACCCTGC GGACAAGATC AGCATTCTGA CGACCTACAA TGGACAGAAG CACCTGATCC 
     4741 GGGACATCAT CAATAGGCGC TGTGGGAATA ATCCACTCAT TGGGAGGCCG AACAAGGTGA 
     4801 CAACTGTTGA TAGGTTCCAA GGTCAACAGA ATGACTACAT CCTCCTTTCT CTAGTGCGGA 
     4861 CCAGGGCCGT GGGCCATTTG AGGGATGTGC GCCGCTTGGT GGTAGCCATG TCGAGAGCCA 
     4921 GGCTTGGCCT GTATATCTTC GCTAGAGTAT CCCTCTTCCA AAATTGCTTT GAACTGACTC 
     4981 CTGCTTTCAG CCAGCTCACT GCCCGCCCAC TGCATTTGCA TATTATTCCA ACAGAACCTT 
     5041 TCCCAACCTC CAGAAAGAAT GGAGAGAGAC CTCCTCATGA AGTACAGGTA ATAAAGAACA 
     5101 TGCCCCAGAT GGCGAATTTT GTGTACAATA TGTACATGCA TTTGATACAG ACGACCCACC 
     5161 ATTATCATCA GACTTTCTTA CAACTACCGC CTGCCATGGT GGAAGAAGGT GAGGAGGTTC 
     5221 AGAGCCAAGA AACAGAAATG GAAGCAGAAG AGGAAACTGG GTCTGTTCAA GCTAACCTCA 
     5281 CACCCAGTCC AACGGATGCC AGCCTGAGTC AAGAGACCCC AACTTCTCAG CCTGACTGCT 
     5341 CCAATCAGAC GGAGGCTGCC TCCAGTCACA CAGAAGATAC CTCTGCTCTG TGTGACATTA 
     5401 CTACTGCAGC CGAGCCTGTC TCTGCAGCGG CAGAAGCTGC CACCCCACAA GATGGGCCTT 
     5461 CTGTTCCCGC AGAGACCAAG ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     5521 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     5581 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     5641 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     5701 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     5761 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     5821 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     5881 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     5941 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     6001 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     6061 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     6121 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     6181 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     6241 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     6301 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     6361 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     6421 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     6481 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     6541 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     6601 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     6661 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     6721 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     6781 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     6841 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     6901 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6961 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     7021 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     7081 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     7141 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     7201 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     7261 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     7321 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     7381 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     7441 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     7501 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     7561 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     7621 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     7681 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     7741 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     7801 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     7861 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     7921 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7981 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     8041 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     8101 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     8161 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     8221 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     8281 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     8341 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     8401 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     8461 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     8521 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     8581 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8641 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8701 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     8761 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     8821 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     8881 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     8941 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     9001 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     9061 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     9121 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     9181 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     9241 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     9301 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//