LOCUS       RR213506                8085 bp    DNA     circular  SYN 
DEFINITION  Lipe (Myc-DDK-tagged ORF) - Rat lipase, hormone sensitive (Lipe), (10 ug)
ACCESSION   NM_012859
VERSION     NM_012859.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4242..4333 
                     /label="Myc-DDK"  
     misc_feature    4363..4948 
                     /label="PolyA signal"  
     misc_feature    4420..4439 
                     /label="Restriction Enzyme"  
     misc_feature    5224..5896 
                     /label="ColE1"  
     misc_feature    6326..7120 
                     /label="Kan/Neo"  
     misc_feature    7155..7457 
                     /label="SV40 ori"  
     misc_feature    7543..8020 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4224..4230 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4224 
                     /label="Lipe"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAACCTAGG AGACCAATTT CTTTCACAAG GGAAATAACA GCTATGGAGC 
     1081 CAAGTTCTAC CTCTGTGTCT AGGCCAGAAT GGAGACCTGA GGCACAGCAG ACCCTCACAG 
     1141 ACTACCCAGG ATCAAGGGAA CTGCAGGAAT TTGGTATCCC ACAGAAACAA AGTCTCCCAA 
     1201 ATGAGGCCAC TGCCCAGCAA GGAGCTGAAT TCCAGCAAGA ACAGGGAGTT CAACAATCTA 
     1261 CTCTGCTCCA GAAACTTCTC ACCCCATTAG CCTTCCCTGT ACCACAGCAA TCATTCCCAA 
     1321 GCCACAAAGT GCACTCGGAT CAACAGGAAG CCACCTCCCA GAATGGACCT GGGGCGGGGA 
     1381 AAGTGCATAC AACTCAAAAG GAACTAGAAC ACAGAGATGA ACACGTGGGG ACAGCAGAAT 
     1441 CTGGACCAGC AGAACCACCT CCAGCTACAG AAGTAGAGGC TACATCTATA GCCCAGGCCG 
     1501 TATCTGGACC GGACAAGAAA CTCCCTACCC AAACTGACTT GGTCTCCCAA GAGAGAGCTG 
     1561 AGCAGTCAGA CCCTACAGCC CAGCAAACAC CTCTAGTCCA AGGAGTCAAG TCTGACCAGG 
     1621 GATCTTTGAT AGAGTCAGGG ATTTTAGCAA GACTGCAGAA ACTAGCCATA CAGCAGCCCT 
     1681 CCCAAGAGTG GAAGACATTT CTTGATTGTG TTACAGAGTC TGACATGGAG AAATACCTGA 
     1741 ATTCATCCTC AAAGTCAAAC CCTCCAGAGC CAAGTGGTGG GACAGTGATC CCAGGAACGC 
     1801 TGCCCTCCAA ACAGAAACCC GATTGTGGAA AGATGTCAGG ATATGGTGGG AAATTACCAC 
     1861 ATGGGAAGAA AGGCATCCTC CAGAAACACA AGCACTACTG GGATACAGCC TCGGCATTCT 
     1921 CACACAGCAT GGATTTACGC ACAATGACAC AGTCGCTGGT GGCGCTCGCA GAAGACAACA 
     1981 TGGCCTTCTT CTCAAGCCAG GGCCCAGGAG AGACAGCACG GCGGCTGTCC AACGTCTTTG 
     2041 CAGGTGTTCG GGAACAGGCA CTGGGGCTAG AACCAACTCT AGGTCAGCTC TTGGGTGTGG 
     2101 CACACCATTT CGACCTGGAC ACTGAGACAC CAGCCAACGG ATACCGTAGC TTGGTGCACA 
     2161 CAGCTCGTTG CTGCCTGGCA CACCTACTAC ACAAATCCCG CTATGTGGCC TCTAACCGCA 
     2221 GAAGTATCTT CTTCCGTGCC AGCCACAACC TAGCAGAACT GGAGGCCTAC CTGGCTGCCC 
     2281 TCACCCAGCT CCGTGCTCTA GCCTACTACG CCCAGCGCCT GCTGACCATC AACCGACCAG 
     2341 GAGTGCTCTT CTTTGAGGGC GATGAGGGAC TCAGCGCTGA CTTCCTACAG GACTATGTCA 
     2401 CGCTACATAA AGGCTGCTTT TACGGTCGCT GCCTGGGCTT CCAGTTCACA CCTGCCATCC 
     2461 GGCCGTTCCT GCAGACTCTC TCCATCGGGC TGGTGTCCTT CGGGGAACAC TACAAACGCA 
     2521 ACGAGACGGG CCTCAGTGTG ACTGCAAGTT CCCTCTTTAC GGGTGGCCGA TTCGCCATAG 
     2581 ACCCAGAGTT GCGTGGGGCT GAATTTGAGC GCATCATACA GAACCTGGAC GTGCACTTCT 
     2641 GGAAAGCCTT CTGGAATATC ACGGAGATCG AGGTGCTATC GTCTCTGGCC AACATGGCAT 
     2701 CAACCACCGT GAGGGTAAGC CGCCTGCTTA GCTTGCCGCC TGAGGCCTTT GAGATGCCAC 
     2761 TAACCTCTGA CCCCAAGCTC ACAGTTACCA TCTCACCTCC CTTGGCACAC ACGGGACCAG 
     2821 GCCCTGTGCT AGCCAGGCTC ATCTCCTATG ACCTGCGGGA AGGGCAGGAC AGCAAGATGC 
     2881 TCAACAGCCT GGCAAAATCT GAGGGCCCAC GCCTGGAGCT GCGCCCACGG CCTCAACAAG 
     2941 CCCCCCGCTC ACGAGCCCTG GTTGTCCACA TTCACGGTGG TGGCTTTGTG GCACAGACCT 
     3001 CCAAATCCCA TGAGCCCTAC CTCAAGAACT GGGCCCAGGA GCTAGGCGTC CCCATCATCT 
     3061 CCATCGACTA CTCCCTGGCC CCTGAGGCCC CCTTCCCCCG AGCACTGGAG GAGTGTTTTT 
     3121 TTGCCTACTG CTGGGCTGTC AAGCACTGTG AACTGCTTGG TTCAACCGGA GAGCGGATAT 
     3181 GCCTTGCGGG GGACAGCGCA GGTGGGAACC TCTGCATCAC CGTGTCCCTT CGGGCAGCAG 
     3241 CCTATGGGGT GAGGGTGCCA GATGGCATCA TGGCAGCCTA CCCAGTTACC ACCCTGCAGT 
     3301 CCTCTGCTTC TCCCTCTCGT CTGCTGAGCC TCATGGATCC TCTTCTACCA CTGAGCGTAC 
     3361 TCTCCAAGTG TGTGAGCGCC TATTCAGGGA CAGAGACGGA GGACCATTTT GACTCAGACC 
     3421 AGAAGGCATT GGGCGTGATG GGGCTGGTGC AGAGAGACAC GTCCCTGTTC CTCAGAGACC 
     3481 TCCGCCTGGG CGCCTCCTCA TGGCTCAACT CCTTCCTGGA GTTAAGTGGG CGCAAGCCCC 
     3541 ATAAGACCCC ATTGCCTGCA ACAGAGACAC TGCGCCCCAC GGATTCTGGG CGCCTTACGG 
     3601 AGTCTATGCG CAGGAGTGTG TCTGAGGCAG CCCTGGCCCA GCCTGAGGGC TTGCTGGGCA 
     3661 CAGATTCCTT GAAGAAGCTA ACAATAAAGG ACTTGAGCTT TAAGGGCAAC TCAGAGCCAT 
     3721 CAGACAGCCC CGAGATGTCA CAGTCAATGG AGACACTTGG CCCCTCCACA CCCTCGGATG 
     3781 TCAACTTTTT TCTGCGATCC GGGAATTCCC AGGAAGAGGC TGAAACCAGA GATGATATAA 
     3841 GCCCCATGGA CGGAATCCCC CGCGTGCGCG CTGCCTTCCC TGATGGTTTC CACCCACGGC 
     3901 GCTCAAGCCA AGGTGTCCTC CACATGCCCC TCTACTCGTC ACCCATAGTC AAGAACCCCT 
     3961 TCATGTCTCC TCTGCTGGCC CCTGACGTCA TGCTGAAGAC CCTGCCGCCC GTGCACCTTG 
     4021 TGGCGTGCGC TCTGGACCCC ATGCTGGATG ACTCGGTCAT GTTCGCGCGG CGACTGAAGG 
     4081 ACCTGGGCCA GCCCGTGACG CTGAAAGTGG TAGAGGACCT GCCGCATGGT TTCCTGAGCT 
     4141 TAGCAGCCCT GTGTCGTGAG ACCCGGCAGG CCGCGGAGTT GTGCGTGCAG CGCATCCGGC 
     4201 TGATCCTCAC CCCCCCTGCT GCACCGCTGA CCACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4261 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4321 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4381 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4441 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4501 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4561 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4621 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4681 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4741 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4801 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4861 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     4921 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     4981 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5041 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5101 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5161 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5221 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5281 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5341 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5401 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5461 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5521 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5581 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5641 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5701 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5761 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5821 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     5881 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     5941 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6001 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6061 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6121 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6181 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6241 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6301 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6361 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6421 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6481 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6541 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6601 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6661 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6721 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6781 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6841 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     6901 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     6961 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7021 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7081 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7141 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7201 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7261 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7321 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7381 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7441 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7501 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7561 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7621 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7681 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7741 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7801 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7861 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     7921 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     7981 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8041 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//