LOCUS       RR213494                9132 bp    DNA     circular  SYN 
DEFINITION  Ncoa1 (Myc-DDK-tagged ORF) - Rat nuclear receptor coactivator 1 (Ncoa1), (10 ug)
ACCESSION   NM_001108012
VERSION     NM_001108012.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5289..5380 
                     /label="Myc-DDK"  
     misc_feature    5410..5995 
                     /label="PolyA signal"  
     misc_feature    5467..5486 
                     /label="Restriction Enzyme"  
     misc_feature    6271..6943 
                     /label="ColE1"  
     misc_feature    7373..8167 
                     /label="Kan/Neo"  
     misc_feature    8202..8504 
                     /label="SV40 ori"  
     misc_feature    8590..9067 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5271..5277 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5271 
                     /label="Ncoa1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGTGGCCTT GGGGACAGTT CATCCGACCC TGCGAACCCA GACTCACATA 
     1081 AGAGAAAAGG ATCGCCATGT GACACACTGG CATCAAGTAC TGAGAAGAGG CGCAGGGAGC 
     1141 AAGAAAATAA GTATTTAGAA GAACTAGCTG AATTACTGTC TGCCAACATC AGTGACATTG 
     1201 ACAGCTTGAG TGTAAAACCA GATAAATGCA AGATCTTGAA GAAGACAGTA GATCAGATAC 
     1261 AGCTAATGAA GAGAATGGAG CAAGAGAAAT CCACAACTGA TGATGACGTG CAGAAGTCCG 
     1321 ACATCTCATC GAGCAGTCAA GGAGTGATAG AGAAGGAGTC GCTGGGCCCC CTTCTTCTGG 
     1381 AGGCTTTGGA TGGATTTTTC TTTGTTGTGA ACTGTGAAGG AAGAATTGTA TTTGTGTCAG 
     1441 AAAATGTGAC CAGCTACCTG GGTTACAATC AGGAAGAATT AATGAATACC AGCGTCTACA 
     1501 GCATACTGCA TGTGGGAGAT CATGCAGAAT TTGTCAAGAA TCTACTACCA AAATCACTAG 
     1561 GCACTGAGAA CCAAGAAGCT TGCCAGCGCT ATGAAGTAAT GCAGTGTTTC ACGGTGTCAC 
     1621 AGCCAAAATC AATCCAAGAA GATGGCGAAG GCAAAATCAT CTCTATTGAC ACTAGCTCCC 
     1681 TGAGAGCTGC TGGCAGGACT GGCTGGGAAG ATTTAGTAAG GAAATGTATC TATGCTTTTT 
     1741 TCCAACCTCA GGGCAGAGAG CCATCTTATG CCCGACAGCT GTTTCAAGAA GTGATGACTC 
     1801 GTGGCACTGC CTCCAGCCCA TCCTATAGAT TCATATTGAA TGATGGGACA ATGCTTAGCG 
     1861 CCCACACCAA GTGTAAACTT TGCTACCCTC AAAGTCCTGA CATGCAGCCT TTCATCATGG 
     1921 GAATTCATAT CATCGACAGG GAGCACAGTG GGCTTTCTCC TCAAGATGAC ACTAATTCTG 
     1981 GAATGTCAAT TCCCCGAATA AACCCCTCAG TCAATCCTGG TATCTCTCCA GCCCATGGTG 
     2041 TGACCCGTTC ATCCACGTTG CCACCATCCA ACAACAACAT GGTCTCTGCC AGAGCAAACC 
     2101 GCCAGCAGAG CACAGACCTT AACAGCGGCA GCAGTCACAG TAACTCTAGC AGCAGCCAAG 
     2161 GGAGCTTTGG ATGCTCACCT GGAAATCAGA TTGTAGCCAG CGTTGCCTTA AGCCAGGGAC 
     2221 AGGCAGGTTC CCAGAGCAGT AATCCGTCTT TAAACCTCAA TAATTCTCCT ATGGAAGGTA 
     2281 CAGGAATTTC CCTCGCACAG TTCATGTCTC CGAGGAGACA AGCTAATTCT GGCTTGGCAA 
     2341 CAAGGGCCAG GATGTCAAAC AATTCATTTC CTCCAAATAT TCCAACATTA AGCTCCCCGG 
     2401 TTGGCATCAC TAGCGGTGCC TGTAGTAATA ATAACCGATC CTATTCCAAT ATCCCAGTAA 
     2461 CATCTTTACA AGGTATGAAT GAAGGACCCA ATAATTCTGT TGGCTTCTCT GCCGGCTCTC 
     2521 CAGTCCTTCG GCAGATGAGC TCACAGAATT CACCTAGCAG ATTAAGTATG CAACCAGCAA 
     2581 AAGCCGAGTC CAAAGACAAC AAAGAGATTG CATCCATTTT AAATGAAATG ATTCAGTCAG 
     2641 ACAACAGTGC TAACGAAGGC AAGCCTCTGG ACTCAGGACT TCTGCATAAC AATGACAGAC 
     2701 TCTCAGATGG AGACAGTAAG TACTCTCAAA CTAGTCACAA ACTAGTACAG CTATTGACGA 
     2761 CTACCGCAGA GCAGCAGTTA CGGCATGCTG ACATAGACAC GAGCTGCAAA GACGTCCTGT 
     2821 CCTGCACTGG CACTTCCAGC TCCGCCTCCT CTAACCCCTC AGGAGGTACT TGTCCCTCTT 
     2881 CCCACAGCTC TCTGACTGAG CGGCATAAAA TTCTGCATCG GCTTTTACAG GAGGGCAGCC 
     2941 CCTCAGACAT CACCACCTTG TCTGTGGAAC CCGAAAAGAA GGACAGTGCG TCAGCTTCTA 
     3001 CTGCTATGTC AGTGTCTGGA CAAGCCCAGG GGAGTGCCAG CATAAAACTA GAACTGGATG 
     3061 CTTCAAAGAA AAAAGAGTCA AAAGACCATC AGCTCCTACG CTACCTTTTA GACAAAGATG 
     3121 AGAAAGATTT AAGATCAACT CCAAACCTGA GCCTGGATGA CGTAAAGGTC AAAGTGGAGA 
     3181 AGAAAGAGCA GATGGATCCT TGTAACACAA ACCCAGCCCC AGTGACCAAA CCTGCTCCCG 
     3241 AGGAGGTTAA ACTCGAGTCC CAGAGCCAGT TTTCAGCTGA CCTTGACCAG TTTGATCAGT 
     3301 TATTGCCCAC ACTGGAGAAG GCAGCGCAGT TGCCGGGCTT GTGTGAGACA GACAGAATGG 
     3361 ATGGTGCGGT CCCCGGTGTG AACATCAAGT CGGAGATCCT GCCCGCTTCA CTTCAGCCCA 
     3421 CCCCTGCCAG ATCTGCTCCC AGGCTGAACA GATTGCCTGA ACTAGAATTA GAAGCAATCG 
     3481 ATAACCAGTT TGGACAACCA GGAGCAGGGG ATCAGATTCC ATGGGCAAAT AATACTGTGA 
     3541 CGGCGATAAA TCAGAGTAAA CCAGAAGACC AATGTATTAG CTCACAGTTG GATGAGCTCC 
     3601 TCTGTCCACC AACAACAGTA GAAGGAAGAA ATGATGAGAA GGCCCTTCTG GAACAACTGG 
     3661 TGTCTTTCCT CAGTGGCAAA GATGAGACGG AGCTGGCCGA GCTAGACAGA GCGCTGGGGA 
     3721 TTGACAAACT TGTACAGGGA GGTGGATTAG ATGTATTATC TGAGAGATTT CCACCACAGC 
     3781 AAGCAACACC ACCTTTGATG ATGGAAGACA GGCCCACCCT CTATCCCCAG CCATACTCGT 
     3841 CTCCTTCTCC CACCGCCGGT CTCTCTGGCC CTTTCCAAGG CATGGTCCGA CAGAAGCCTT 
     3901 CACTGGGTAC TATGCCGGTT CAAGTAACAC CTCCTCGAGG AACCTTTTCA CCCAACATGG 
     3961 GCATGCAACC CCGGCAGACT CTAAATAGAC CTCCAGCTGC ACCCAACCAG CTTCGGCTGC 
     4021 AGCTGCAGCA GCGGCTACAG GGGCAGCAGC AGTTGATGCA CCAAAACCGG CAAGCTATCT 
     4081 TGAACCAGTT TGCAGCAAAT GCTCCTGTTG GCATGAACAT GAGGTCAGGC ATGCAGCAGC 
     4141 AGATAACACC TCAGCCACCC CTGAATGCTC AGATGTTGGC ACAGCGCCAA CGGGAGCTGT 
     4201 ACAGTCAGCA GCATCGACAG AGGCAAATTA TCCAGCAGCA AAGAGCCATG CTCATGAGGC 
     4261 ACCAAAGCTT TGGGAACAAC ATCCCTCCTT CATCCGGACT CCCAGTTCAA ATGGGGAACC 
     4321 CCCGTCTTCC TCAGGGTGCT CCACAGCAGT TCCCCTACCC ACCAAACTAT GGTACAAATC 
     4381 CAGGAACCCC ACCTGCTTCT ACCAGCCCTT TCTCTCAACT GGCAGCAAAC CCTGAGGCAT 
     4441 CCTTGGCCAC CCGCAGCAGC ATGGTGAACA GAGGCATGGC AGGGAACATG GGAGGACAGT 
     4501 TTGGTACTGG CATCAGTCCG CAGATGCAGC AGAATGTCTT CCAGTACCCA GGGTCAGGAC 
     4561 TGGTTCCCCA AGCTGAGGCC AACTTTGCCC CATCTCTAAG CCCTGGGAGC TCCATGGTGC 
     4621 CGATGCCAAT CCCTCCTCAA AGCTCTCTGC TCCAACAAAC TCCACCCACT TCTGGGTACC 
     4681 AGTCACCGGA CATGAAGGCC TGGCAGCAAG GAACAATGGG AAACAACAAT GTGTTCAGTC 
     4741 AAGCCGTCCA GAGCCAGCCT GCCCCTGCAC AGCCAGGAGT GTACAACAAC ATGAGCATCA 
     4801 CCGTGTCCAT GGCAGGTGGA AACGCAAACG TTCAGAACAT GAATCCAATG ATGGGCCAGA 
     4861 TGCAAATGAG CTCTCTGCAG ATGCCAGGGA TGAACACTGT GTGCTCTGAG CAGATGAATG 
     4921 ATCCAGCACT GAGACACACA GGCCTCTACT GCAACCAGCT CTCATCCACT GACCTTCTCA 
     4981 AAACCGAAGC AGATGGAAAC CAGGACAAGA AGACAGAAGA GTTCTTCTCT GTGCAGGTGC 
     5041 AGCAGCTTCA GGTGTTTGCT GACGTCCAGT GTACAGTGAA CCTGAAAGGA GCCAGCAAAC 
     5101 CGACTCTAGT GCTAGCAGTT CTGCTACAAG AAAAGGCCAT AAGAACCAAT GCAGATGAGG 
     5161 AGCATGGACT CAAGACTTCA AGGAAGAAGC CATTTCCCCA GGTCCTTCCT TCTGCATCTC 
     5221 ACCGCCCCTG GTACAAACAA CTCCATAAGA ACAGCATCTA CTCAGAAACT ACAGGACTTA 
     5281 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     5341 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     5401 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     5461 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     5521 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     5581 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     5641 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     5701 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     5761 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     5821 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     5881 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     5941 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     6001 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     6061 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     6121 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     6181 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     6241 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     6301 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     6361 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     6421 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     6481 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     6541 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     6601 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     6661 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     6721 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     6781 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     6841 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     6901 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     6961 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     7021 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     7081 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     7141 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     7201 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     7261 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     7321 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     7381 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     7441 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     7501 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     7561 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     7621 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     7681 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     7741 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     7801 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     7861 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     7921 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     7981 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     8041 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     8101 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     8161 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     8221 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     8281 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     8341 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     8401 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     8461 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     8521 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     8581 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     8641 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     8701 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     8761 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     8821 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     8881 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     8941 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     9001 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     9061 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     9121 AACGCGAATT TT
//