LOCUS       RR213465                8157 bp    DNA     circular  SYN 
DEFINITION  Zfp521 (Myc-DDK-tagged ORF) - Rat zinc finger protein 521 (Znf521), (10 ug)
ACCESSION   NM_001107403
VERSION     NM_001107403.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4314..4405 
                     /label="Myc-DDK"  
     misc_feature    4435..5020 
                     /label="PolyA signal"  
     misc_feature    4492..4511 
                     /label="Restriction Enzyme"  
     misc_feature    5296..5968 
                     /label="ColE1"  
     misc_feature    6398..7192 
                     /label="Kan/Neo"  
     misc_feature    7227..7529 
                     /label="SV40 ori"  
     misc_feature    7615..8092 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4296..4302 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4296 
                     /label="Zfp521"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GCAGGTTCAT GAGCGGAGCA AGGACGGCTC CCAGTCTGGC TCCAGGATGG 
     1081 AGGACTGGAA GATGAAGGAC ACTCAGAAGT GCAGCCAGTG TGAGGAAGGC TTTGACTTCC 
     1141 CAGAAGACCT CCAGAAGCAC ATCGCAGAGT GCCACCCCGA GTGCTCCCCA AATGAGGACA 
     1201 GGGCAGCCCT CCAGTGTGTG TACTGCCACG AGCTGTTTGT GGAGGAGACC TCCCTCATGA 
     1261 ACCACATAGA GCAGGTGCAC GGGGGTGAGA AAAAGAACTC ATGTAGCATA TGCTCAGAGA 
     1321 GCTTCCTCAC TGTCGAGGAG CTGTATAGCC ACATGGACAG CCACCAGCAA CCAGAGTCCT 
     1381 GCAATCACAG CAACAGCCCT TCCCTGGTCA CCGTGGGATA CACCTCTGTG TCCAGCACCA 
     1441 CTCCAGATTC CAACCTCTCA GTGGACAGCT CAACCATGGT GGAGGCCGCA CCACCAATTC 
     1501 CAAAGAGCCG CGGGAGGAAG CGAGCTGCCC AGCAGACCTC CGATATGACT GGCCCCTCCA 
     1561 GTAAACAGGC AAAAGTCACC TACAGCTGCA TTTACTGCAA CAAGCAGTTA TTCTCGAGTC 
     1621 TCGCGGTCCT GCAGATTCAC TTGAAAACTA TGCATTTAGA TAAGCCGGAG CAGGCTCATA 
     1681 TCTGTCAGTA TTGCTTGGAG GTCTTGCCCT CACTCTATAA CCTAAATGAA CATCTTAAAC 
     1741 AAGTGCACGA AGCTCAGGAC CCGGGCCTGA TTGTTTCGGC CATGCCTGCC ATCATTTACC 
     1801 AGTGCAACTT CTGTTCCGAA GTTGTCAATG ACCTCAACAC CCTTCAGGAG CACATCCGAT 
     1861 GTTCTCACGG GTTTGCCAAT CCCGCAGCCA AGGACAGCAA TGCTTTCTTT TGTCCCCATT 
     1921 GTTACATGGG GTTTCTCACA GACTCTTCCC TTGAAGAGCA TATAAGGCAG GTCCACTGTG 
     1981 ACCTCAGCGG CTCCCGGTTT GGGTCTCCTG TGCTTGGGAC TCCGAAAGAA CCGGTAGTTG 
     2041 AAGTCTACTC CTGTTCCTAT TGTACAAACT CGCCAATATT CAACAGTGTT CTTAAACTGA 
     2101 ATAAGCATAT TAAAGAGAAT CATAAAAACA TTCCCTTGGC CCTGAATTAT ATTCACAATG 
     2161 GGAAGAAATC CCGGGCCCTG AGCCCCTTGT CTCCTGTGGC TATAGAACAG ACATCGCTTA 
     2221 AGATGATGCA GACAGTGGGA GGTGGCCCTG CCCGTGCTGC TGGAGAATAT ATCTGTAACC 
     2281 AGTGTGGTGC TAAGTACACG TCCCTAGACA GCTTTCAGAC TCACCTCAAA ACCCATCTGG 
     2341 ACACCGTGCT GCCGAAACTG ACCTGCCCTC AGTGTAACAA AGAGTTCCCC AATCAGGAGT 
     2401 CCCTGCTGAA GCACGTGACC ATCCACTTCA TGATCACCTC AACCTACTAC ATCTGCGAGA 
     2461 GCTGCGACAA GCAGTTCACC TCCGTGGACG ACCTCCAGAA GCACCTGCTG GACATGCACA 
     2521 CCTTCGTGTT CTTCCGCTGC ACGCTGTGCC AGGAGGTGTT CGACTCGAAG GTGTCCATCC 
     2581 AGCTGCACCT GGCCGTGAAG CACAGTAACG AGAAGAAAGT GTACCGCTGC ACGTCCTGCA 
     2641 ACTGGGACTT CCGCAACGAG ACCGACCTGC AGCTGCACGT GAAGCACAAC CACCTGGAGA 
     2701 ACCAGGGCAA GGTGCACAAG TGCATCTTCT GTGGCGAGTC TTTCGGCACC GAGGTGGAGC 
     2761 TGCAGTGCCA CATCACCACG CACAGCAAGA AGTACAACTG CAGGTTCTGC AGCAAGGCCT 
     2821 TCCACGCAGT CCTCCTGCTG GAGAAGCACC TGCGGGAGAA GCACTGCGTG TTTGAAACCA 
     2881 AGACCCCCAA CTGCGGCACC AACGGAGCCT CGGAGCAGGT GCAGAAGGAG GAGGCCGAGC 
     2941 TGCAGACCTT GCTCACCAAC AGCCAGGAGT CCCATAACAG CCACGACGGG AGCGAGGAGG 
     3001 ACGTGGACAG CTCCGAACCC ATGTATGGCT GCGACATCTG CGGGGCGGCC TACACCATGG 
     3061 AGACTCTGCT GCAGAACCAC CAGCTCCGGG ATCACAACAT CCGACCGGGA GAGAGTGCCA 
     3121 TCGTGAAGAA GAAGGCGGAG CTCATTAAAG GGAATTACAA GTGCAACGTG TGCTCGAGAA 
     3181 CCTTCTTCTC TGAGAACGGG CTCCGTGAGC ACATGCAGAC TCACTTAGGC CCTGTCAAAC 
     3241 ACTACATGTG CCCCATCTGT GGAGAGCGCT TCCCTTCCCT TTTGACTCTG ACCGAACACA 
     3301 AAGTCACGCA CAGCAAGAGC TTGGATACTG GAAACTGTCG CATTTGCAAG ATGCCCCTGC 
     3361 AGAGCGAAGA GGAGTTCTTA GAGCATTGTC AGATGCACCC TGACCTGAGG AATTCCCTCA 
     3421 CCGGGTTTCG CTGCGTGGTG TGCATGCAGA CTGTGACCTC CACCTTGGAA CTCAAAATCC 
     3481 ACGGGACCTT CCACATGCAG AAGACGGGGA ACGGGTCCTC CGTGCAGACC ACCGGGCGTG 
     3541 GCCAGCACGT TCAGAAACTG TATAAGTGCG CATCTTGCCT CAAAGAGTTC CGTTCCAAGC 
     3601 AAGATCTGGT GAAACTTGAT ATCAACGGCC TGCCATATGG TCTCTGTGCC GGCTGTGTGA 
     3661 ATCTCAGTAA GAGCAGTAGC CCGGGCCTCG GTCTCCCTCC TGTTGCGAGC AGACCAGGCT 
     3721 TGGGCCAGAA TGAGAGTCTG AGCGCCATGG AAGGAAAAGG CAAGGCAGGG GGTCTGAAGA 
     3781 CGACTCGCTG TTCAAGCTGC AATGTCAAGT TTGAGTCTGA AAGCGAACTG CAGAACCACA 
     3841 TCCAGACGGT GCACCGGGAG CTCGTGCCAG ACGCCAACAG CACACAGTTG AAAACGCCCC 
     3901 AAGTGTCACC CATGCCCAGA ATCAGTCCCT CCCAGTCAGA TGAGAAGAAG ACCTACCAGT 
     3961 GTATCAAGTG TCAGATGGTT TTCTACAACG AGTGGGACAT CCAGGTTCAT GTGGCAAATC 
     4021 ACATGATTGA TGAAGGGCTG AACCATGAAT GCAAACTCTG CAGCCAGACC TTTGACTCCC 
     4081 CTGCCAAACT TCAGTGTCAC CTGATAGAGC ACAGCTTCGA AGGGATGGGA GGCACCTTCA 
     4141 AGTGCCCCGT CTGTTTCACA GTGTTTGTCC AAGCTAACAA GTTGCAGCAG CACATATTCT 
     4201 CCGCCCATGG ACAAGAAGAC AAAATCTATG ACTGCACACA ATGCCCTCAG AAGTTTTTCT 
     4261 TCCAAACAGA GCTACAGAAT CATACGATGA CTCAGCATAG CAGTACGCGT ACGCGGCCGC 
     4321 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     4381 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4441 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4501 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4561 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4621 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4681 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4741 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4801 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4861 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4921 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4981 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     5041 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     5101 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     5161 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     5221 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     5281 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     5341 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     5401 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5461 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5521 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5581 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5641 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5701 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5761 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5821 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5881 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5941 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     6001 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     6061 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     6121 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     6181 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     6241 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     6301 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     6361 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6421 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6481 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6541 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6601 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6661 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6721 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6781 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6841 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6901 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6961 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     7021 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     7081 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     7141 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     7201 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     7261 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     7321 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     7381 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7441 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7501 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7561 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7621 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7681 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7741 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7801 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7861 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7921 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7981 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     8041 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     8101 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//