LOCUS       RR213448                8745 bp    DNA     circular  SYN 
DEFINITION  Oplah (Myc-DDK-tagged ORF) - Rat 5-oxoprolinase (ATP-hydrolysing) (Oplah), (10 ug)
ACCESSION   NM_053904
VERSION     NM_053904.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4902..4993 
                     /label="Myc-DDK"  
     misc_feature    5023..5608 
                     /label="PolyA signal"  
     misc_feature    5080..5099 
                     /label="Restriction Enzyme"  
     misc_feature    5884..6556 
                     /label="ColE1"  
     misc_feature    6986..7780 
                     /label="Kan/Neo"  
     misc_feature    7815..8117 
                     /label="SV40 ori"  
     misc_feature    8203..8680 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4884..4890 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4884 
                     /label="Oplah"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCAGCCCA GAAGGGCGCT TCCATTTCGC CATCGACCGC GGTGGCACCT 
     1081 TCACAGATGT CTTTGCCCAG TGCCCTGGAG GGCATGTGCG TGTCCTGAAG CTGCTCTCAG 
     1141 AGGACCCTGC CAACTATCCA GATGCACCCA CAGAGGGCAT CCGCCGAATT CTAGAGCAGG 
     1201 AGGAGGGTGT GCTGCTGCCT CGAGGCCGAC CGCTAGACAC CAGTCGCATT GCCAGCATCC 
     1261 GCATGGGTAC CACGGTGGCC ACCAATGCAC TGTTGGAACG ACAGGGAGAA CGGGTGGCAC 
     1321 TGCTGGTGAC TCGGGGTTTC CGAGACCTGC TGCATATTGG CACTCAGGCC CGCCCGGACC 
     1381 TCTTTGACTT GGCTGTGCCC ATGCCAGAGG TTCTGTATGA GGAAGTGCTG GAGGTAGATG 
     1441 AGCGAGTGGT GCTGTATCGC GGAGAACCAG GTGCCGGCTC TCCTGTCAAA GGCCGCACAG 
     1501 GGGACCTGCT AGAGATACAG CAGCCTGTGG ACCTGGAAGC CCTGCGTGGG AAGCTGGAGG 
     1561 GGCTCTTGTC TCGGGGCATT CACAGTCTGG CAGTGGTGCT CATGCATTCG TACACGTGGG 
     1621 CCCAGCATGA GCAGCAGGTG GGCACGCTGG CCCGGGAGCT GGGCTTCACG CACGTCTCCT 
     1681 TGTCCTCGGA AGTCATGCCC ATGGTACGAA TTGTTCCTCG GGGCCATACA GCCTGTGCTG 
     1741 ACGCTTACCT TACTCCCACC ATCCAGCGCT ATGTGCAGGG CTTCCGCCGA GGCTTCCAGG 
     1801 GCCAGCTAAA GAATGTGCAA GTTCTCTTCA TGCGCTCTGA TGGTGGCCTC GCACCCATGG 
     1861 ATGCTTTCAG TGGTTCCCGG GCTGTGCTCT CTGGCCCTGC TGGGGGTGTG GTTGGCTACT 
     1921 CAGCTACCAC CTACCATCTG GAAGGCGGTC AGCCTGTCAT TGGCTTTGAC ATGGGAGGCA 
     1981 CATCCACAGA CGTGAGCCGC TATGCTGGAG AATTTGAGCA TGTCTTTGAG GCTAGCACAG 
     2041 CAGGCGTTAC CCTTCAGGCA CCCCAGTTGG ACATCAACAC AGTGGCAGCT GGCGGGGGTT 
     2101 CCCGCCTCTT CTTCAGATCT GGCCTCTTTG TGGTTGGTCC AGAGTCAGCA GGTGCCCACC 
     2161 CAGGTCCTGC CTGCTACCGT AAAGGGGGTC CTGTGACAGT GACAGATGCT AATCTGGTCC 
     2221 TGGGTCGCCT GCTGCCTGCC TCCTTCCCCT GCATTTTTGG GCCAGGAGAA GACCAGCCAC 
     2281 TGTCTCCTGA GGCTTCCCGA AAGGCTCTAG AGGCTGTGGC CATGGAGGTC AACAGTTTCT 
     2341 TGACCAATGG ACCGTGCCCA GCTTCCCAAC TAAGTCTGGA AGAGGTGGCC ATGGGGTTTG 
     2401 TGCGTGTTGC CAATGAAGCC ATGTGCCGGC CTATCCGTGC CCTCACACAG GCACGAGGCC 
     2461 ATGACCCCTC AGCCCATGTA TTGGCTTGCT TTGGAGGAGC TGGTGGGCAA CACGCTTGTG 
     2521 CCATTGCCCG GGCCCTGGGG ATGGATACTG TGCACATTCA CAGGCACAGC GGGCTGCTGT 
     2581 CAGCACTAGG ACTGGCCTTG GCAGATGTGG TTCACGAAGC ACAGGAGCCC TGTTCCCTGT 
     2641 CTTACACACC TGAAACCTTT GCACAACTGG ACCAGAGACT GAGCCGCCTG GAGGAGCAGT 
     2701 GTGTGGATGC CTTGCAGGTC CAGGGCTTCC CTAGGTCTCA GATCAGCACC GAGAGCTTCC 
     2761 TGCATCTTCG CTACCAAGGC ACTGACTGCG CCCTAATGGT GTCTGCCCAT CAGCATCCGG 
     2821 CCACAGCCTG CTCACCCCGA GCTGGTGACT TTGGAGCCGC ATTTGTGGAG AGGTACATGA 
     2881 GAGAGTTTGG CTTCATTATC CCCGAGCGGC CGGTGGTGGT AGATGATGTA CGTGTGAGGG 
     2941 GAACTGGCCG TAGTGGACTT CAGCTGGAGG ACACCCCCAA AATCCAGACT GGACCTCCCC 
     3001 ACGTGGAAAA GGTGACCCAG TGCTACTTTG AAGGGGGTTA TCAGGAGACT CCCGTGTACC 
     3061 TTTTAGGAGA ACTAGGCTAC GGGCACCAGC TCCAAGGGCC CTGCCTTATC ATCGACAACA 
     3121 ACAGCACCAT CCTTGTAGAA CCGGGTTGCC AAGCAGAGGT GACTGATACA GGGGACATCC 
     3181 GCATTTCTGT GGGAGCTGAG GGTCCTAGTA TGGCAGATAC CAGGCTTGAC CCCATCCAGC 
     3241 TGTCTATTTT CTCACACCGC TTCATGAGCA TTGCTGAGCA GATGGGCCGC ATCCTACAGC 
     3301 GCACAGCCAT CTCTACCAAC ATCAAGGAAC GCCTCGACTT CTCCTGTGCC CTCTTTGGGC 
     3361 CAGATGGGGG CCTCGTCTCC AATGCTCCCC ACATTCCTGT GCACCTGGGT GCCATGCAAG 
     3421 AGACTGTACA GTTCCAGATT CAGCACTTAG GAGCCGACCT CCATCCTGGT GATGTGTTGC 
     3481 TCAGCAACCA TCCCAGCGCA GGGGGCAGCC ATCTTCCTGA CCTGACTGTC ATTACACCGG 
     3541 TGTTTTGGCC AGGCCAGACG AGGCCTGTGT TCTACGTGGC TAGCCGAGGG CACCACGCAG 
     3601 ACATTGGAGG AATCACACCG GGCTCTATGC CGCCTCACTC CACCACGCTG CAACAGGAGG 
     3661 GTGCCGTTTT TCTGTCCTTC AAACTGGTCC AGGGAGGCGT CTTCCAGGAA GAGGCAGTGA 
     3721 CAGAGGCCCT ACGGGCACCA GGCAAGATCT CTGGCTGTAG TGGAACCAGG AACCTGCATG 
     3781 ACAACCTGTC GGATCTTCGT GCCCAGGTGG CAGCTAACCA GAAAGGCATC CAGCTGGTGG 
     3841 GAGAGCTGAT CGGACAGTAT GGCTTAGATG TGGTGCAGGC CTATATGGGC CATATTCAGG 
     3901 CGAATGCTGA GCTAGCAGTG AGAGACATGC TCCGGGCTTT TGGAACTTCC CGGCAGGCCA 
     3961 GGGGCCTGCC CCTGGAGGTG TCTGCAGAGG ATCACATGGA TGATGGCTCT CCCATCTGTC 
     4021 TGCGTGTTCA GATCAACCTG AGTCAGGGCA GTGCGGTATT TGACTTTACT GGTTCCGGGT 
     4081 CTGAGGTGTT TGGCAATCTC AATGCCCCGA GAGCCATAAC ACTGTCTGCT CTCATCTATT 
     4141 GCTTACGCTG TCTAGTGGGC CGTGACATCC CACTTAACCA GGGTTGCCTG GCTCCTGTGC 
     4201 GTGTCATAAT TCCCAAAGGC TCCATATTGG ATCCATCCCC AGAGGCAGCA GTGGTCGGCG 
     4261 GCAACGTGCT CACATCTCAG CGAGTAGTGG ATGTCATTCT GGGGGCTTTT GGGGCCTGTT 
     4321 CAGCCTCCCA GGGCTGCATG AACAATGTGA CCCTGGGCAA TGCCCGTATG GGCTACTATG 
     4381 AGACAGTGGC TGGTGGTGCC GGTGCGGGCC CTGGCTGGCA TGGGCGCAGT GGTGTACACA 
     4441 GTCACATGAC CAACACACGC ATTACGGATC CAGAGATTCT GGAGAGTCGG TATCCAGTTA 
     4501 TCCTGCGCCG CTTTGAGCTG AGGCCAGGCT CCGGGGGCCG AGGTCGCTTC CGGGGAGGTG 
     4561 ATGGCGTAGT CCGAGAGCTG GTCTTTCGGG AAGAGGCGCT GTTGTCTGTG CTCACCGAGC 
     4621 GCCGGGCCTT CCAGCCTTAC GGCCTCCACG GGGGAGAGCC TGGTGCGCGT GGCTTAAACC 
     4681 TCCTGATCAG AAAAGATGGG CGCACAGTGA ATTTGGGCGG CAAGACATCT GTGACCGTGT 
     4741 ACCCCGGGGA CGTGTTCTGC CTCCACACGC CTGGGGGTGG GGGCTACGGA GACCCGGAGG 
     4801 ATCCAGCGCC ACCACCAGGC TCGCCCCCGC TATTTCCAGC CTTCCCCGAG CGCGGCAGTG 
     4861 TATTCGAGTA CCGCCGCGCC CAGGAAGCCG TAACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4921 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4981 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     5041 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     5101 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     5161 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     5221 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     5281 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     5341 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     5401 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     5461 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     5521 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5581 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5641 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5701 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5761 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5821 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5881 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5941 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     6001 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     6061 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     6121 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     6181 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     6241 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     6301 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     6361 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     6421 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     6481 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6541 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6601 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6661 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6721 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6781 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6841 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6901 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6961 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     7021 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     7081 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     7141 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     7201 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     7261 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     7321 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     7381 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     7441 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     7501 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7561 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7621 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7681 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7741 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7801 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7861 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7921 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7981 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     8041 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     8101 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     8161 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     8221 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     8281 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     8341 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     8401 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     8461 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     8521 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8581 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8641 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8701 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//