LOCUS       RR213405                7332 bp    DNA     circular  SYN 
DEFINITION  Tpcn1 (Myc-DDK-tagged ORF) - Rat two pore segment channel 1 (Tpcn1), (10 ug)
ACCESSION   NM_139332
VERSION     NM_139332.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3489..3580 
                     /label="Myc-DDK"  
     misc_feature    3610..4195 
                     /label="PolyA signal"  
     misc_feature    3667..3686 
                     /label="Restriction Enzyme"  
     misc_feature    4471..5143 
                     /label="ColE1"  
     misc_feature    5573..6367 
                     /label="Kan/Neo"  
     misc_feature    6402..6704 
                     /label="SV40 ori"  
     misc_feature    6790..7267 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3471..3477 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3471 
                     /label="Tpcn1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCTGTAATT TTAGACGACG ACGTGCTGCT CATCCTGACC TTGGACGAGG 
     1081 AGCTGAGTGC GCCGCTGACT CCTTCCAACG GCCTGGGCCA AGAAGACCTG CCCAGCAAAA 
     1141 ATGGGGGCGG CCAAAGCGGC CCCAACTCCC AAGTTCCCAG TCTGGTCTCT GGAGCCGACA 
     1201 GTCCCCCCTC CAGTCCCCCC GGACACAACT GGGAGATGAA TTATCAAGAG GCAGCAATCT 
     1261 ACCTCCAGGA AGGCCAGAAC AATGACAAGT TCTTCACCCA CCCCAAGGAT GCCAGAGCGC 
     1321 TGGCTGCCTA CCTCTTCGTA CACAACCACT TCTTTTATAT GATGGAGCTG CTCACAGCCC 
     1381 TGCTTCTGCT GCTGCTGTCA CTGTGCGAGT CACCCGCTGT CCCTGCGCTC AAGCTCCGAA 
     1441 CTTACGTCCA CGCCACCCTG GAGCTGTTCG CCCTCATGGT GGTGGTATTT GAACTCTGCA 
     1501 TGAAATTGCG GTGGCTGGGC TTCCACACAT TCGTCAGGCA CAAACGCACC ATGGTCAAGA 
     1561 CGTCTGTTTT GGTGGTGCAG TTCATCGAGG CCATTGTGGT GCTGGTGCGG CAGACGTCCC 
     1621 ACGTGCGGGT GACCCGGGCA CTACGCTGCA TTTTCCTGGT GGACTGTCGG TACTGTGGTG 
     1681 GCGTGCGGCG CAACCTGCGG CAGATCTTCC AGTCGCTGCC GCCTTTCATG GACATCCTCC 
     1741 TGCTGCTGCT CTTCTTTATG ATCATCTTCG CCATCCTCGG TTTCTACTTA TTCTCTACGA 
     1801 ACCCTTCCGA CCCTTACTTC AACACCCTGG AGAACAGCAT CGTCAACCTG TTTGTTCTCC 
     1861 TGACCACAGC CAACTTTCCA GACGTCATGA TGCCCTCCTA CTCCCGGAAC CCCTGGTCCT 
     1921 GTGTCTTCTT TATCGTGTAC CTCTCCATTG AGCTGTACTT CATCATGAAC CTGCTCCTAG 
     1981 CCGTGGTGTT CGACACCTTC AACGACATCG AAAAGCACAA GTTCAAGTCC TTGCTGCTGC 
     2041 ATAAGCGAAC AGCCATCCAG CACGCATACC ACCTGCTCGT CAGCCAACGG AGGCCGGCCG 
     2101 GCATCTCCTA CAGGCAGTTT GAAGGCTTGA TGCGCTTCTA CAAGCCCCGG ATGAGCGCTA 
     2161 GGGAGCGCTT CCTGACTTTC AAGGCCCTGA ATCAGAGCAA CACACCTCTG CTTAGCCTGA 
     2221 AGGACTTCTA TGATATTTAT GAAGTTGCTG CTCTGCAGTG GAAGGCAAAG AAGAACAGAC 
     2281 AGCACTGGTT TGACGAGCTT CCCCGGACAG CCTTCCTCAT CTTCAAAGGG ATTAACATCC 
     2341 TTGTGAATTC CAAGGCCTTC CAGTATTTCA TGTACCTGGT GGTGGCTGTC AACGGTGTCT 
     2401 GGATCCTAGT GGAGACATTC ATGTTGAAAG GTGGGAATTT CATCTCAAAG CACGTGCCCT 
     2461 GGAGTTACCT CGTGTTTCTT ACCATCTATG GAGTTGAGCT GTTCATGAAG GTGGCTGGCC 
     2521 TGGGCCCTGT GGAGTACTTG TCCTCTGGAT GGAACCTGTT CGATTTCTCA GTCACAGCAT 
     2581 TTGCCTTCCT GGGACTGCTC GCGCTGACGC TCAACATGGA GCCCTTCTAC TTTATTGTGG 
     2641 TCCTGCGTCC CCTTCAGCTG CTGAGGTTAT TCAAGTTGAA GAAACGCTAC CGCAATGTGT 
     2701 TGGACACCAT GTTTGAGCTG CTGCCACGAA TGGCCAGCCT CGGCCTCACA CTGCTCACCT 
     2761 TCTACTACTC CTTCGCCATC GTGGGCATGG AGTTCTTCAG TGGGCGACTG TCCCCCAACT 
     2821 GCTGCAACTC CAGCACGGTG GCTGACGCCT ATCGATTCAT CAATCACACG GTGGGCAATA 
     2881 AGACCAAGGT GGAGGAAGGC TACTACTATC TCAATAACTT TGACAACATC CTCAACAGCT 
     2941 TTGTGACCTT GTTTGAGCTC ACCGTTGTCA ACAACTGGTA CATCATCATG GAAGGCGTCA 
     3001 CCTCGCAGAC TTCCCACTGG AGCCGCCTGT ACTTCATGAC CTTTTACATC GTGACCATGG 
     3061 TGGTGATGAC CATTATCGTG GCCTTCATCC TGGAGGCCTT CGTCTTCCGC ATGAACTACA 
     3121 GCCGCAAGAG CCAGGAATCG GAAGTGGACA GTGGCATCGT CATCGAGAAG GAGATGTCCA 
     3181 AGGAGGAGCT TCTGGCCATC CTGGAGCTTC ATCGAGAGGC GCGAGGCACC TCCTCTGACA 
     3241 TCACCCGGCT GCTCGACACC CTCTCTCAGA TGGAGAAATA CCAGCAAAAC TCCATGGTGT 
     3301 TTCTGGGACG GCGATCGAGA ACCAAAAGTG ACCTGAGCCT GAAGATGTAC CAGGAGGAGA 
     3361 TCCAGGAGTG GTACGAGGAG CATGCCCGGG AACAGGAGCA GCAGCAGCTC AGGGGCAGCG 
     3421 CGCCAAGCCC TGCAGCCCAG CAGACCCCCG GCAGTCGCCA GCGCTCCCAG ACCGTCACCA 
     3481 CGCGTACGCG GCCGCTCGAG CAGAAACTCA TCTCAGAAGA GGATCTGGCA GCAAATGATA 
     3541 TCCTGGATTA CAAGGATGAC GACGATAAGG TTTAAACGGC CGGCCGCGGT CATAGCTGTT 
     3601 TCCTGAACAG ATCCCGGGTG GCATCCCTGT GACCCCTCCC CAGTGCCTCT CCTGGCCCTG 
     3661 GAAGTTGCCA CTCCAGTGCC CACCAGCCTT GTCCTAATAA AATTAAGTTG CATCATTTTG 
     3721 TCTGACTAGG TGTCCTTCTA TAATATTATG GGGTGGAGGG GGGTGGTATG GAGCAAGGGG 
     3781 CAAGTTGGGA AGACAACCTG TAGGGCCTGC GGGGTCTATT GGGAACCAAG CTGGAGTGCA 
     3841 GTGGCACAAT CTTGGCTCAC TGCAATCTCC GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT 
     3901 CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA TGCATGACCA GGCTCAGCTA ATTTTTGTTT 
     3961 TTTTGGTAGA GACGGGGTTT CACCATATTG GCCAGGCTGG TCTCCAACTC CTAATCTCAG 
     4021 GTGATCTACC CACCTTGGCC TCCCAAATTG CTGGGATTAC AGGCGTGAAC CACTGCTCCC 
     4081 TTCCCTGTCC TTCTGATTTT AAAATAACTA TACCAGCAGG AGGACGTCCA GACACAGCAT 
     4141 AGGCTACCTG GCCATGCCCA ACCGGTGGGA CATTTGAGTT GCTTGCTTGG CACTGTCCTC 
     4201 TCATGCGTTG GGTCCACTCA GTAGATGCCT GTTGAATTGG GTACGCGGCC AGCGGCGAGC 
     4261 GGTATCAGCT CACTCAAAGG CGGTAATACG GTTATCCACA GAATCAGGGG ATAACGCAGG 
     4321 AAAGAACATG TGAGCAAAAG GCCAGCAAAA GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT 
     4381 GGCGTTTTTC CATAGGCTCC GCCCCCCTGA CGAGCATCAC AAAAATCGAC GCTCAAGTCA 
     4441 GAGGTGGCGA AACCCGACAG GACTATAAAG ATACCAGGCG TTTCCCCCTG GAAGCTCCCT 
     4501 CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT TACCGGATAC CTGTCCGCCT TTCTCCCTTC 
     4561 GGGAAGCGTG GCGCTTTCTC ATAGCTCACG CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT 
     4621 TCGCTCCAAG CTGGGCTGTG TGCACGAACC CCCCGTTCAG CCCGACCGCT GCGCCTTATC 
     4681 CGGTAACTAT CGTCTTGAGT CCAACCCGGT AAGACACGAC TTATCGCCAC TGGCAGCAGC 
     4741 CACTGGTAAC AGGATTAGCA GAGCGAGGTA TGTAGGCGGT GCTACAGAGT TCTTGAAGTG 
     4801 GTGGCCTAAC TACGGCTACA CTAGAAGAAC AGTATTTGGT ATCTGCGCTC TGCTGAAGCC 
     4861 AGTTACCTTC GGAAAAAGAG TTGGTAGCTC TTGATCCGGC AAACAAACCA CCGCTGGTAG 
     4921 CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT TACGCGCAGA AAAAAAGGAT CTCAAGAAGA 
     4981 TCCTTTGATC TTTTCTACGG GGTCTGACGC TCAGTGGAAC GAAAACTCAC GTTAAGGGAT 
     5041 TTTGGTCATG AGATTATCAA AAAGGATCTT CACCTAGATC CTTTTAAATT AAAAATGAAG 
     5101 TTTTAAATCA ATCTAAAGTA TATATGAGTA ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC 
     5161 GACGTTGGCT GCGAGCCCTG GGCCTTCACC CGAACTTGGG GGGTGGGGTG GGGAAAAGGA 
     5221 AGAAACGCGG GCGTATTGGC CCCAATGGGG TCTCGGTGGG GTATCGACAG AGTGCCAGCC 
     5281 CTGGGACCGA ACCCCGCGTT TATGAACAAA CGACCCAACA CCGTGCGTTT TATTCTGTCT 
     5341 TTTTATTGCC GTCATAGCGC GGGTTCCTTC CGGTATTGTC TCCTTCCGTG TTTCAGTTAG 
     5401 CCTCCCCCTA GGGTGGGCGA AGAACTCCAG CATGAGATCC CCGCGCTGGA GGATCATCCA 
     5461 GCCGGCGTCC CGGAAAACGA TTCCGAAGCC CAACCTTTCA TAGAAGGCGG CGGTGGAATC 
     5521 GAAATCTCGT GATGGCAGGT TGGGCGTCGC TTGGTCGGTC ATTTCGAACC CCAGAGTCCC 
     5581 GCTCAGAAGA ACTCGTCAAG AAGGCGATAG AAGGCGATGC GCTGCGAATC GGGAGCGGCG 
     5641 ATACCGTAAA GCACGAGGAA GCGGTCAGCC CATTCGCCGC CAAGCTCTTC AGCAATATCA 
     5701 CGGGTAGCCA ACGCTATGTC CTGATAGCGA TCCGCCACAC CCAGCCGGCC ACAGTCGATG 
     5761 AATCCAGAAA AGCGGCCATT TTCCACCATG ATATTCGGCA AGCAGGCATC GCCATGGGTC 
     5821 ACGACGAGAT CCTCGCCGTC GGGCATGCTC GCCTTGAGCC TGGCGAACAG TTCGGCTGGC 
     5881 GCGAGCCCCT GATGCTCTTC GTCCAGATCA TCCTGATCGA CAAGACCGGC TTCCATCCGA 
     5941 GTACGTGCTC GCTCGATGCG ATGTTTCGCT TGGTGGTCGA ATGGGCAGGT AGCCGGATCA 
     6001 AGCGTATGCA GCCGCCGCAT TGCATCAGCC ATGATGGATA CTTTCTCGGC AGGAGCAAGG 
     6061 TGAGATGACA GGAGATCCTG CCCCGGCACT TCGCCCAATA GCAGCCAGTC CCTTCCCGCT 
     6121 TCAGTGACAA CGTCGAGCAC AGCTGCGCAA GGAACGCCCG TCGTGGCCAG CCACGATAGC 
     6181 CGCGCTGCCT CGTCTTGCAG TTCATTCAGG GCACCGGACA GGTCGGTCTT GACAAAAAGA 
     6241 ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC ACGGCGGCAT CAGAGCAGCC GATTGTCTGT 
     6301 TGTGCCCAGT CATAGCCGAA TAGCCTCTCC ACCCAAGCGG CCGGAGAACC TGCGTGCAAT 
     6361 CCATCTTGTT CAATCATGCG AAACGATCCT CATCCTGTCT CTTGATCGAT CTTTGCAAAA 
     6421 GCCTAGGCCT CCAAAAAAGC CTCCTCACTA CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC 
     6481 CTCGGCCTCT GCATAAATAA AAAAAATTAG TCAGCCATGG GGCGGAGAAT GGGCGGAACT 
     6541 GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT TAGGGGCGGG ACTATGGTTG CTGACTAATT 
     6601 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCTGGT 
     6661 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6721 TCCACACCCT AACTGACACA CATTCCACAG CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT 
     6781 TTTCAATATT ATTGAAGCAT TTATCAGGGT TATTGTCTCA TGAGCGGATA CATATTTGAA 
     6841 TGTATTTAGA AAAATAAACA AATAGGGGTT CCGCGCACAT TTCCCCGAAA AGTGCCACCT 
     6901 GACGCGCCCT GTAGCGGCGC ATTAAGCGCG GCGGGTGTGG TGGTTACGCG CAGCGTGACC 
     6961 GCTACACTTG CCAGCGCCCT AGCGCCCGCT CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC 
     7021 ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA AATCGGGGGC TCCCTTTAGG GTTCCGATTT 
     7081 AGTGCTTTAC GGCACCTCGA CCCCAAAAAA CTTGATTAGG GTGATGGTTC ACGTAGTGGG 
     7141 CCATCGCCCT GATAGACGGT TTTTCGCCCT TTGACGTTGG AGTCCACGTT CTTTAATAGT 
     7201 GGACTCTTGT TCCAAACTGG AACAACACTC AACCCTATCT CGGTCTATTC TTTTGATTTA 
     7261 TAAGGGATTT TGCCGATTTC GGCCTATTGG TTAAAAAATG AGCTGATTTA ACAAAAATTT 
     7321 AACGCGAATT TT
//