LOCUS       RR213353                8007 bp    DNA     circular  SYN 
DEFINITION  Lats2 (Myc-DDK-tagged ORF) - Rat large tumor suppressor 2 (Lats2), (10 ug)
ACCESSION   NM_001107267
VERSION     NM_001107267.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4164..4255 
                     /label="Myc-DDK"  
     misc_feature    4285..4870 
                     /label="PolyA signal"  
     misc_feature    4342..4361 
                     /label="Restriction Enzyme"  
     misc_feature    5146..5818 
                     /label="ColE1"  
     misc_feature    6248..7042 
                     /label="Kan/Neo"  
     misc_feature    7077..7379 
                     /label="SV40 ori"  
     misc_feature    7465..7942 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4146..4152 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4146 
                     /label="Lats2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCCAAAG ACTTTTCCTG CTACAACTTA CTCTGGAAAT AGCCGGCAGC 
     1081 GACTGCAAGA GATTCGAGAA GGACTGAAGC AGCCGGCCAA GTCTTCCACC CAGGGGCTGC 
     1141 TGGTGGGACC AAACAGTGAC ACTTCTTCCC TGGATGCCAA AGTCCTGGGG AACAAAGATG 
     1201 CCTCCAGGCA GCAGCAGATG AGAGCCACCC CGAAGTTTGG ACCTTATCAA AAAGCGCTCA 
     1261 GGGAAATCCG ATATTCCCTC CTGCCTTTTG CCAATGAGTC AGGCACGTCG GCAGCTGCAG 
     1321 AGGTGAACCG GCAGATGCTG CAGGAGTTGG TGAATGCAGG ATGTGACCAG GAGATGGCTG 
     1381 GCAGAGCGCT CAAGCAGACA GGCAGTAGGA GTATTGAAGC CGCCTTGGAG TACATCAGTA 
     1441 AGATGGGCTA CTTGGACCCC AGGAATGAGC AAATTGTGCG AGTCATCAAG CAGACCTCCC 
     1501 CAGGAAAGGG CCTGGTGCCC ACTGCAGTGA CTCGACGACC CAGCTTTGAG GGCACAGGGG 
     1561 AAGCATTCCC ATCCCACCAC CAGTTGGGCG GTGCAAACTA TGAGGGCCCC GCTGCCCTGG 
     1621 AGGAGATGCC ACGGCAATAT TTAGACTTCC TCTTCCCCGG GGCCAGCGTC CACGGCGCCC 
     1681 CGGCTCACCA GCATCCCCCC AAAGGCTACA GCACTGCAGT GGATGCGGGC ACACACTTCC 
     1741 CAGGCGCACA CTATGGTCGT GCTCATCTAC TGTCAGAGCA GCCTGGGTAT GGGGTTCAGC 
     1801 GCAGCCCCTC CTTCCAGAGC AAGACGCCAC CAGACACCTA TTCCAGCCTG GCCAAGGCCC 
     1861 AGGGTGGCCC TCCTGCCAGC CTCACCTTCC CTGCCCATGC TGGTCTGTAC ACCACCTCGC 
     1921 ACCACAAGCC AGCGGCTGCA GTACCTGGGG CCCACCCCTT ACATGTGTTG GGCACCCGGG 
     1981 GCCCCACGTT CACTGGGGAC AGCTCTACGC AGGCCGTTCT GGCACCGTCC AGGAACAGCC 
     2041 TCAATAATGA CTTGTACGAG CTGGGCTCTC CCGTGCCCTG GCCCGCAGCT CCACTGGCAC 
     2101 GCCGTGACTC GCTGCAGAAG CAGAGTCTAG AGGCCTCCCG GCCGCATGTG GCCTTCCGGG 
     2161 CTGGGCCCAG CAGGACCAAC TCCTTCAGCA GCCCACAACC TGAGCCCTCA CTGCCCGCCC 
     2221 CCAACACAGT CACTGCTGTG ACGGCTGCAC ACATCCTTCA CCCTGTGAAG AGCGTGCGCG 
     2281 TGCTGCGGCC CGAGCCTCAG ACAGCCGTGG GGCCCTCGCA CCCCGCCTGG GTGCCTGCCC 
     2341 CCACAGCACC TGCAGCCGCC GAGGGCCTGG AGAGCAAGGA GGGCAGTGCA GGCCCACCCC 
     2401 CGCTGGATAT GGACTTCGGG GGCTCCGAGC GCAGGTGCCC ACCGCCTCCT TACCCAAAGC 
     2461 ACTTGCTGCT GCCCAGTAAG TCTGAGCAGT ACAGCGCTGG TCTGGACCGC CTGTGCACCG 
     2521 GTGTGGAGCA GAGTCTGCGC GGGGGCACCG AGCAAGACAG GAATGACAAG GGCCACAAGG 
     2581 GTGCTAAGGG GGACAAAGCT GGCAGGGACA AAAAGCAGAT CCAGACCTCC CCAGTGCCTG 
     2641 TCCGCAAGAA TAGCAGGGAT GAAGAGAAGA GAGAGTCTCG AATCAAGAGT TACTCCCCAT 
     2701 ATGCCTTCAA ATTCTTCATG GAACAACATG TGGAGAATGT CATCAAAACC TACCAGCAGA 
     2761 AGGTCAGCCG GAGGCTACAG CTGGAGCAGG AAATGGCCAA AGCTGGACTG TGCGAGACTG 
     2821 AGCAGGAGCA GATGAGGAAG ATCCTCTACC AGAAGGAGTC GAACTACAAC AGACTCAAGA 
     2881 GGGCCAAGAT GGACAAGTCC ATGTTTGTGA AAATCAAAAC TCTGGGCATT GGTGCCTTTG 
     2941 GAGAAGTGTG CCTTGCCTGT AAGGTGGATA CTCACGCTCT GTATGCCATG AAGACGCTGA 
     3001 GGAAAAAAGA TGTCCTGAAC CGGAACCAAG TGGCCCATGT CAAGGCTGAG AGGGACATCC 
     3061 TGGCTGAAGC AGACAATGAG TGGGTGGTCA AACTCTACTA TTCCTTCCAG GACAAGGACA 
     3121 GCCTGTACTT TGTGATGGAC TACATACCTG GTGGGGACAT GATGAGCCTG CTGATCCGGA 
     3181 TGGAGGTCTT CCCTGAACAC CTCGCCCGCT TCTACATCGC AGAGCTGACT CTGGCCATTG 
     3241 AGAGTGTGCA CAAGATGGGC TTCATCCACA GGGACATCAA GCCTGACAAC ATCTTGATTG 
     3301 ACCTGGATGG TCATATTAAG CTGACCGATT TTGGCCTCTG CACTGGATTC AGGTGGACTC 
     3361 ACAATTCCAA GTACTACCAG AAAGGGAACC ACATCAGACA GGACAGCATG GAGCCTGGTG 
     3421 ACCTCTGGGA CGACGTTTCC AACTGTCGTT GTGGAGACAG GTTGAAGACC CTGGAGCAGA 
     3481 GAGCACAGAA GCAGCACCAG AGGTGCCTGG CACATTCTCT GGTTGGGACA CCAAATTACA 
     3541 TTGCTCCTGA GGTGCTTCTT CGCAAAGGGT ACACACAGCT CTGTGACTGG TGGAGTGTCG 
     3601 GTGTGATTCT TTTTGAGATG CTGGTTGGGC AGCCACCTTT CTTGGCGCCC ACCCCCACAG 
     3661 AAACCCAGCT GAAGGTGATC AACTGGGAGA ACACGCTGCA CATCCCTACG CAGGTGAAAC 
     3721 TCAGTGCTGA GGCCCGAGAC CTCATCACCA AGCTGTGCTG CGCGGCTGAC TGCCGCCTGG 
     3781 GCAGGGACGG GGCAGATGAC CTCAAGGCAC ACCCGTTCTT CAGCACCATC GACTTTTCCC 
     3841 GTGACATCCG GAAACAGCCT GCACCCTACG TCCCCACCAT CAGCCACCCC ATGGACACCT 
     3901 CCAATTTTGA CCCAGTGGAT GAAGAAAGCC CCTGGCACGA GGCCAGCGGA GAAAGTGCCA 
     3961 AGGCCTGGGA CACGCTGGCG TCCCCCAACA GCAAGCACCC AGAGCACGCC TTCTATGAGT 
     4021 TCACCTTCCG CAGGTTCTTC GACGACAATG GCTATCCCTT CCGGTGCCCG AAGCCCTCAG 
     4081 AGCCGGCTGA GAGTGCAGAC CCAGGGGATG CAGAGTTAGG AGGTGCGGTC GAGGGCTGCC 
     4141 AGCCAGTGTA TGTGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA GAAGAGGATC 
     4201 TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA ACGGCCGGCC 
     4261 GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC CTCCCCAGTG 
     4321 CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT AATAAAATTA 
     4381 AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG GAGGGGGGTG 
     4441 GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT CTATTGGGAA 
     4501 CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC CTGGGTTCAA 
     4561 GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT GACCAGGCTC 
     4621 AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG GCTGGTCTCC 
     4681 AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG ATTACAGGCG 
     4741 TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA GCAGGAGGAC 
     4801 GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT GAGTTGCTTG 
     4861 CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA ATTGGGTACG 
     4921 CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT CCACAGAATC 
     4981 AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA GGAACCGTAA 
     5041 AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC ATCACAAAAA 
     5101 TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC AGGCGTTTCC 
     5161 CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG GATACCTGTC 
     5221 CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA GGTATCTCAG 
     5281 TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG TTCAGCCCGA 
     5341 CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC ACGACTTATC 
     5401 GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG GCGGTGCTAC 
     5461 AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT TTGGTATCTG 
     5521 CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT CCGGCAAACA 
     5581 AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC GCAGAAAAAA 
     5641 AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT GGAACGAAAA 
     5701 CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT AGATCCTTTT 
     5761 AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG AGGCTATGGC 
     5821 AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC TTGGGGGGTG 
     5881 GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG GTGGGGTATC 
     5941 GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC CAACACCGTG 
     6001 CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT 
     6061 CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA GATCCCCGCG 
     6121 CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC TTTCATAGAA 
     6181 GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT CGGTCATTTC 
     6241 GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC GATGCGCTGC 
     6301 GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC GCCGCCAAGC 
     6361 TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC CACACCCAGC 
     6421 CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT CGGCAAGCAG 
     6481 GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG 
     6541 AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG ATCGACAAGA 
     6601 CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG GTCGAATGGG 
     6661 CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT GGATACTTTC 
     6721 TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC CAATAGCAGC 
     6781 CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC GCCCGTCGTG 
     6841 GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC GGACAGGTCG 
     6901 GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC GGCATCAGAG 
     6961 CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA AGCGGCCGGA 
     7021 GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC TGTCTCTTGA 
     7081 TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT GGAATAGCTC 
     7141 AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC CATGGGGCGG 
     7201 AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG GCGGGACTAT 
     7261 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     7321 CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7381 GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT TCTTTCCGCC 
     7441 TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG TCTCATGAGC 
     7501 GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG CACATTTCCC 
     7561 CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG TGTGGTGGTT 
     7621 ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC 
     7681 CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG GGGGCTCCCT 
     7741 TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA TTAGGGTGAT 
     7801 GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC 
     7861 ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC TATCTCGGTC 
     7921 TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA AAATGAGCTG 
     7981 ATTTAACAAA AATTTAACGC GAATTTT
//