LOCUS       RR213267                9015 bp    DNA     circular  SYN 
DEFINITION  Cpd (Myc-DDK-tagged ORF) - Rat carboxypeptidase D (Cpd), (10 ug)
ACCESSION   NM_012836
VERSION     NM_012836.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5172..5263 
                     /label="Myc-DDK"  
     misc_feature    5293..5878 
                     /label="PolyA signal"  
     misc_feature    5350..5369 
                     /label="Restriction Enzyme"  
     misc_feature    6154..6826 
                     /label="ColE1"  
     misc_feature    7256..8050 
                     /label="Kan/Neo"  
     misc_feature    8085..8387 
                     /label="SV40 ori"  
     misc_feature    8473..8950 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5154..5160 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5154 
                     /label="Cpd"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGAGCGGC TGGGATGAGC GGCCGCCCTG GCGGCTGGAA AGCCTGCGGC 
     1081 TGCTACCGCC GCCACCGCTG CTGCTGCTAC TGCTGCTATT GAGGAGCTCG GCCCAGGCGG 
     1141 CGCACATCAA GAAGGCTGAG GCGACCACGA CGACCGTCGG CGGCTCCGAG GCGGCCGAGG 
     1201 GCCAGTTTGA CCACTACTAC CACGAAGCGG CGCTGGGCGA GGCACTGGAG GCGGCAGCGG 
     1261 CCGCGGGGCC GCCTGGCCTG GCCCGTCTCT TCAGCATCGG CAATTCGGTG GAGGGCCGAC 
     1321 CGCTGTGGGT GCTGCGCCTC ACGGCTGGCC TGGGGCCGCC GCCCACCCCC GCCGCCGTGG 
     1381 GGCTGGACGC CGCGGGGCCG CTGCTGCCCG GCCGACCGCA GGTGAAGCTG GTGGGTAACA 
     1441 TGCACGGCGA CGAGACGGTG TCGCGCCAGG TGTTGGTCTA CCTGGCCCGT GAGCTGGCGT 
     1501 CGGGCTACCG CCGCGGGGAC CCGCGCCTGG TCCGCCTGCT CAACACCACC GACGTATATT 
     1561 TGCTGCCCAG CCTGAATCCG GATGGCTTCG AACGCGCTCG CGAGGGTGAC TGCGGCCTCG 
     1621 GCGACAGCGG TCCGCCTGGG ACCAGCGGCC GAGACAACAG CCGAGGCCGC GACCTCAACC 
     1681 GCAGCTTCCC TGACCAGTTC AGCACGGGGG AGCCACCTTC CCTGGATGAG GTGCCAGAGG 
     1741 TGCGCGCCCT CATCGACTGG ATCCGCAGGA ACAAGTTTGT GCTTTCTGGA AATCTTCATG 
     1801 GTGGCTCAGT GGTAGCAAGC TATCCTTTTG ATGATTCTCC CGAGCATAAG ACCACTGGAA 
     1861 TCTATAGCAA AACCTCCGAT GATGAAGTCT TTCGATACTT GGCGAAAGCT TATGCTTCTA 
     1921 ACCACCCTAT AATGAGAACT GGTGAACCCC ATTGTCCAGG AGATGAAGAG GAGACTTTCA 
     1981 AAGACGGGAT CACGAACGGT GCGCACTGGT ATGATGTGGA AGGTGGTATG CAAGATTACA 
     2041 ACTATGTGTG GGCCAACTGT TTTGAGATCA CATTAGAATT ATCTTGCTGC AAATACCCAC 
     2101 CTGCTTCTCA GCTTCGACAA GAATGGGAGA ACAACCGTGA GTCTTTGATC ACATTGATTG 
     2161 AAAAGGTCCA CATTGGAATT AAAGGATTTG TTAAAGACTC AGTCACAGGC TCTGGGTTGG 
     2221 AGAATGCAAC CATCTCAGTG GCTGGTATTA ATCATAATAT CACAACTGGG AGATTCGGTG 
     2281 ATTTCCACAG ACTGCTCATT CCTGGAAGTT ACAATCTTAC AGCGGTTTCA CCTGGGTATA 
     2341 TGCCGTTGAC TATTAATAAT ATAGTGGTGA AAGAAGGACC AGCCACAGAG ATAGATTTCT 
     2401 CCCTTCAACC AACTGTGATG TCAGTAGTGC CTGACTCGAC TGAGGCTGTG ACAACTCCCG 
     2461 GCACAGTTGC CGTTCCTAAT ATTCCTCCTG GAACACCATC GTCCCATCAG CCAATTCAGC 
     2521 CTAAAGACTT TCACCACCAC CATTTTCCTG ACATGGAAAT CTTCTTGAGA AGATTTGCCA 
     2581 ATGAATATCC TAACATCACT CGCCTTTATT CTCTGGGAAA GTCCGTAGAG TCGAGAGAAC 
     2641 TGTATGTGAT GGAGATATCT GACAACCCAG GCGTCCATGA ACCAGGTGAA CCAGAATTTA 
     2701 AATATATTGG AAATATGCAT GGAAATGAAG TGGTTGGAAG AGAACTGCTA TTGAATCTCA 
     2761 TCGAATACCT CTGCAAGAAC TTTGGAACAG ACCCTGAAGT AACAGATTTG GTTCGCAGCA 
     2821 CTAGAATTCA CCTCATGCCA TCCATGAATC CTGATGGGTA TGAGAAGTCC CAAGAAGGAG 
     2881 ACTCGATAAG TGTAGTTGGC AGAAACAACA GCAACAACTT TGACCTAAAC CGAAATTTCC 
     2941 CAGACCAGTT TGTTCCGATC ACAGATCCTA CCCAACCTGA AACTATCGCT GTGATGAGCT 
     3001 GGGTGAAGGC CTATCCGTTT GTGCTGTCGG CCAATCTGCA TGGAGGTTCC TTGGTGGTTA 
     3061 ACTACCCTTA TGATGATAAT GAACAAGGAG TTGCCACATA CAGCAAATCA CCAGATGACG 
     3121 CCGTGTTTCA ACAAATAGCA CTTTCTTATT CCAAGGAAAA CTCGCAGATG TTCCAAGGTA 
     3181 GACCTTGCAA AGACATGTAC CTTAATGAGT ATTTTCCTCA TGGCATAACG AATGGAGCGA 
     3241 GTTGGTATAA TGTCCCAGGT GGTATGCAGG ACTGGAACTA TTTACAGACG AATTGCTTTG 
     3301 AAGTAACCAT CGAACTAGGT TGTGTGAAAT ATCCATTCGA GAAGGAACTG CCAAAGTACT 
     3361 GGGAGCAAAA CCGGAGATCT CTAATCCAGT TTATGAAACA GGTTCATCAG GGTGTCAAAG 
     3421 GATTTGTTCT TGATGCCACA GATGGTAGGG GCATATTAAA TGCCACCCTC AGTGTTGCTG 
     3481 AAATTAATCA CCCAGTGACG ACTTACAAAG CTGGAGATTA CTGGCGTCTC TTGGTCCCAG 
     3541 GGACATATAA AATCACAGCA TCTGCTCGAG GGTATAACCC CGTCACCAAG AACGTGACCG 
     3601 TCAGGAGTGA AGGTGCTATT CAGGTCAACT TCACACTTGT TCGATCTTCA ACAGATGCAA 
     3661 ACAATGAGTC AAAGAAAGGA AAGGGGCATA GCACCAGCAC TGATGACACC AGCGACCCAA 
     3721 CTTCTAAGGA GTTTGAAGCA CTGATCAAAC ACCTTTCGGC TGAGAATGGC TTGGAAGGCT 
     3781 TCATGTTAAG CTCGTCCTCA GACCTGGCTC TGTATCGATA CCATTCCTAC AAAGACTTAT 
     3841 CCGAGTTCCT GAGAGGACTC GTAATGAACT ACCCACACAT TACAAACCTT ACCACTTTGG 
     3901 GACAGAGTGT TGAATATCGT CACATTTGGT CCCTTGAAAT CTCCAATAAG CCCAACATAT 
     3961 CAGAACCTGA AGAACCGAAG ATTCGTTTTG TTGCTGGTAT CCATGGAAAC GCTCCAGTTG 
     4021 GCACTGAACT GCTTTTGGCT CTGGCAGAAT TTCTTTGCCT GAATTACAAA AAGAATCCAG 
     4081 TTGTTACCCA ATTGGTTGAC AGGACTAGAA TTGTGATTGT CCCCTCTCTG AATCCAGATG 
     4141 GGAGAGAGCG AGCACAGGAG AAAGACTGTA CCTCGAAGAC TGGGCACACA AATGCCCGTG 
     4201 GCAGGGACCT GGACACAGAC TTCACAAGTA ATGCTTCCCA ACCTGAGACC AAAGCCATCA 
     4261 TTGAAAACTT GATTCAAAAA CAGGATTTCA GCCTTTCCAT TGCTTTAGAT GGTGGTTCTG 
     4321 TGCTGGTCAC ATATCCGTAC GACAAGCCAG TCCAGACAGT GGAAAACAAA GAAACGTTAA 
     4381 AGCATCTGGC GTCTCTTTAT GCAAATAACC ACCCATCAAT GCACATGGGT CAGCCCAGTT 
     4441 GCCCAAATAA TTCAGATGAG AACATACCAG GAGGAGTGAT GCGGGGAGCA GAGTGGCACA 
     4501 GTCACCTGGG CAGCATGAAG GATTATAGTG TCACCTATGG CCATTGCCCA GAAATCACAG 
     4561 TATACACAAG CTGCTGCTAC TTCCCAAGTG CAGCTCAACT CCCTGCCCTA TGGGCAGAGA 
     4621 ACAAGAAGTC TCTCCTCAGC ATGCTGGTGG AGGTCCACAA AGGAGTCCAT GGGTTAGTAA 
     4681 AAGACAAGAC TGGTAAACCA ATCTCTAAAG CAGTAATTGT ACTCAATGAA GGAATAAGAG 
     4741 TACACACAAA AGAGGGGGGT TATTTCCATG TGCTTTTAGC TCCTGGGGTC CATAACATCA 
     4801 ATGCCATCGC TGATGGATAT CAGCAACAGC ATTCACAGGT CTTTGTGCAT CATGATGCAG 
     4861 CCAGTTCTGT GGTGATAGTC TTTGATACAG ATAACCGGAT ATTTGGTTTG CCAAGGGAAC 
     4921 TTGTGGTAAC TGTGTCAGGT GCCACCATGT CAGCGCTGAT CCTCACGGCT TGCATCATTT 
     4981 GGTGCATCTG CTCTATCAAG TCTAACAGAC ACAAAGATGG CTTCCACCGG CTGCGGCAGC 
     5041 ATCATGATGA ATATGAAGAT GAAATTCGCA TGATGTCAAC TGGCTCTAAG AAGTCCCTTT 
     5101 TAAGCCATGA GTTCCAGGAT GAAACGGACA CAGAGGAAGA GACATTATAT TCTAGCAAAC 
     5161 ATACGCGTAC GCGGCCGCTC GAGCAGAAAC TCATCTCAGA AGAGGATCTG GCAGCAAATG 
     5221 ATATCCTGGA TTACAAGGAT GACGACGATA AGGTTTAAAC GGCCGGCCGC GGTCATAGCT 
     5281 GTTTCCTGAA CAGATCCCGG GTGGCATCCC TGTGACCCCT CCCCAGTGCC TCTCCTGGCC 
     5341 CTGGAAGTTG CCACTCCAGT GCCCACCAGC CTTGTCCTAA TAAAATTAAG TTGCATCATT 
     5401 TTGTCTGACT AGGTGTCCTT CTATAATATT ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG 
     5461 GGGCAAGTTG GGAAGACAAC CTGTAGGGCC TGCGGGGTCT ATTGGGAACC AAGCTGGAGT 
     5521 GCAGTGGCAC AATCTTGGCT CACTGCAATC TCCGCCTCCT GGGTTCAAGC GATTCTCCTG 
     5581 CCTCAGCCTC CCGAGTTGTT GGGATTCCAG GCATGCATGA CCAGGCTCAG CTAATTTTTG 
     5641 TTTTTTTGGT AGAGACGGGG TTTCACCATA TTGGCCAGGC TGGTCTCCAA CTCCTAATCT 
     5701 CAGGTGATCT ACCCACCTTG GCCTCCCAAA TTGCTGGGAT TACAGGCGTG AACCACTGCT 
     5761 CCCTTCCCTG TCCTTCTGAT TTTAAAATAA CTATACCAGC AGGAGGACGT CCAGACACAG 
     5821 CATAGGCTAC CTGGCCATGC CCAACCGGTG GGACATTTGA GTTGCTTGCT TGGCACTGTC 
     5881 CTCTCATGCG TTGGGTCCAC TCAGTAGATG CCTGTTGAAT TGGGTACGCG GCCAGCGGCG 
     5941 AGCGGTATCA GCTCACTCAA AGGCGGTAAT ACGGTTATCC ACAGAATCAG GGGATAACGC 
     6001 AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA AAAGGCCAGG AACCGTAAAA AGGCCGCGTT 
     6061 GCTGGCGTTT TTCCATAGGC TCCGCCCCCC TGACGAGCAT CACAAAAATC GACGCTCAAG 
     6121 TCAGAGGTGG CGAAACCCGA CAGGACTATA AAGATACCAG GCGTTTCCCC CTGGAAGCTC 
     6181 CCTCGTGCGC TCTCCTGTTC CGACCCTGCC GCTTACCGGA TACCTGTCCG CCTTTCTCCC 
     6241 TTCGGGAAGC GTGGCGCTTT CTCATAGCTC ACGCTGTAGG TATCTCAGTT CGGTGTAGGT 
     6301 CGTTCGCTCC AAGCTGGGCT GTGTGCACGA ACCCCCCGTT CAGCCCGACC GCTGCGCCTT 
     6361 ATCCGGTAAC TATCGTCTTG AGTCCAACCC GGTAAGACAC GACTTATCGC CACTGGCAGC 
     6421 AGCCACTGGT AACAGGATTA GCAGAGCGAG GTATGTAGGC GGTGCTACAG AGTTCTTGAA 
     6481 GTGGTGGCCT AACTACGGCT ACACTAGAAG AACAGTATTT GGTATCTGCG CTCTGCTGAA 
     6541 GCCAGTTACC TTCGGAAAAA GAGTTGGTAG CTCTTGATCC GGCAAACAAA CCACCGCTGG 
     6601 TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA GATTACGCGC AGAAAAAAAG GATCTCAAGA 
     6661 AGATCCTTTG ATCTTTTCTA CGGGGTCTGA CGCTCAGTGG AACGAAAACT CACGTTAAGG 
     6721 GATTTTGGTC ATGAGATTAT CAAAAAGGAT CTTCACCTAG ATCCTTTTAA ATTAAAAATG 
     6781 AAGTTTTAAA TCAATCTAAA GTATATATGA GTAACCTGAG GCTATGGCAG GGCCTGCCGC 
     6841 CCCGACGTTG GCTGCGAGCC CTGGGCCTTC ACCCGAACTT GGGGGGTGGG GTGGGGAAAA 
     6901 GGAAGAAACG CGGGCGTATT GGCCCCAATG GGGTCTCGGT GGGGTATCGA CAGAGTGCCA 
     6961 GCCCTGGGAC CGAACCCCGC GTTTATGAAC AAACGACCCA ACACCGTGCG TTTTATTCTG 
     7021 TCTTTTTATT GCCGTCATAG CGCGGGTTCC TTCCGGTATT GTCTCCTTCC GTGTTTCAGT 
     7081 TAGCCTCCCC CTAGGGTGGG CGAAGAACTC CAGCATGAGA TCCCCGCGCT GGAGGATCAT 
     7141 CCAGCCGGCG TCCCGGAAAA CGATTCCGAA GCCCAACCTT TCATAGAAGG CGGCGGTGGA 
     7201 ATCGAAATCT CGTGATGGCA GGTTGGGCGT CGCTTGGTCG GTCATTTCGA ACCCCAGAGT 
     7261 CCCGCTCAGA AGAACTCGTC AAGAAGGCGA TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG 
     7321 GCGATACCGT AAAGCACGAG GAAGCGGTCA GCCCATTCGC CGCCAAGCTC TTCAGCAATA 
     7381 TCACGGGTAG CCAACGCTAT GTCCTGATAG CGATCCGCCA CACCCAGCCG GCCACAGTCG 
     7441 ATGAATCCAG AAAAGCGGCC ATTTTCCACC ATGATATTCG GCAAGCAGGC ATCGCCATGG 
     7501 GTCACGACGA GATCCTCGCC GTCGGGCATG CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT 
     7561 GGCGCGAGCC CCTGATGCTC TTCGTCCAGA TCATCCTGAT CGACAAGACC GGCTTCCATC 
     7621 CGAGTACGTG CTCGCTCGAT GCGATGTTTC GCTTGGTGGT CGAATGGGCA GGTAGCCGGA 
     7681 TCAAGCGTAT GCAGCCGCCG CATTGCATCA GCCATGATGG ATACTTTCTC GGCAGGAGCA 
     7741 AGGTGAGATG ACAGGAGATC CTGCCCCGGC ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC 
     7801 GCTTCAGTGA CAACGTCGAG CACAGCTGCG CAAGGAACGC CCGTCGTGGC CAGCCACGAT 
     7861 AGCCGCGCTG CCTCGTCTTG CAGTTCATTC AGGGCACCGG ACAGGTCGGT CTTGACAAAA 
     7921 AGAACCGGGC GCCCCTGCGC TGACAGCCGG AACACGGCGG CATCAGAGCA GCCGATTGTC 
     7981 TGTTGTGCCC AGTCATAGCC GAATAGCCTC TCCACCCAAG CGGCCGGAGA ACCTGCGTGC 
     8041 AATCCATCTT GTTCAATCAT GCGAAACGAT CCTCATCCTG TCTCTTGATC GATCTTTGCA 
     8101 AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA CTACTTCTGG AATAGCTCAG AGGCCGAGGC 
     8161 GGCCTCGGCC TCTGCATAAA TAAAAAAAAT TAGTCAGCCA TGGGGCGGAG AATGGGCGGA 
     8221 ACTGGGCGGA GTTAGGGGCG GGATGGGCGG AGTTAGGGGC GGGACTATGG TTGCTGACTA 
     8281 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCT 
     8341 GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG AGCCTGGGGA 
     8401 CTTTCCACAC CCTAACTGAC ACACATTCCA CAGCTGGTTC TTTCCGCCTC AGGACTCTTC 
     8461 CTTTTTCAAT ATTATTGAAG CATTTATCAG GGTTATTGTC TCATGAGCGG ATACATATTT 
     8521 GAATGTATTT AGAAAAATAA ACAAATAGGG GTTCCGCGCA CATTTCCCCG AAAAGTGCCA 
     8581 CCTGACGCGC CCTGTAGCGG CGCATTAAGC GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG 
     8641 ACCGCTACAC TTGCCAGCGC CCTAGCGCCC GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC 
     8701 GCCACGTTCG CCGGCTTTCC CCGTCAAGCT CTAAATCGGG GGCTCCCTTT AGGGTTCCGA 
     8761 TTTAGTGCTT TACGGCACCT CGACCCCAAA AAACTTGATT AGGGTGATGG TTCACGTAGT 
     8821 GGGCCATCGC CCTGATAGAC GGTTTTTCGC CCTTTGACGT TGGAGTCCAC GTTCTTTAAT 
     8881 AGTGGACTCT TGTTCCAAAC TGGAACAACA CTCAACCCTA TCTCGGTCTA TTCTTTTGAT 
     8941 TTATAAGGGA TTTTGCCGAT TTCGGCCTAT TGGTTAAAAA ATGAGCTGAT TTAACAAAAA 
     9001 TTTAACGCGA ATTTT
//