LOCUS       RR213232                6053 bp    DNA     circular  SYN 
DEFINITION  Gja1 (Myc-DDK-tagged ORF) - Rat gap junction protein, alpha 1 (Gja1), (10 ug)
ACCESSION   NM_012567
VERSION     NM_012567.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2210..2301 
                     /label="Myc-DDK"  
     misc_feature    2331..2916 
                     /label="PolyA signal"  
     misc_feature    2388..2407 
                     /label="Restriction Enzyme"  
     misc_feature    3192..3864 
                     /label="ColE1"  
     misc_feature    4294..5088 
                     /label="Kan/Neo"  
     misc_feature    5123..5425 
                     /label="SV40 ori"  
     misc_feature    5511..5988 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2192..2198 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2192 
                     /label="Gja1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGTGACTGG AGTGCCTTGG GGAAGCTTCT GGACAAGGTC CAAGCCTACT 
     1081 CCACCGCTGG AGGGAAGGTG TGGCTGTCAG TGCTCTTCAT ATTCAGAATC CTGCTCCTGG 
     1141 GGACAGCTGT TGAGTCAGCT TGGGGTGATG AACAGTCTGC CTTTCGCTGT AACACTCAAC 
     1201 AACCTGGCTG CGAAAACGTC TGCTATGACA AGTCCTTCCC CATCTCTCAC GTGCGCTTCT 
     1261 GGGTCCTTCA GATCATATTC GTGTCTGTGC CCACCCTCCT GTACTTGGCC CATGTGTTCT 
     1321 ATGTGATGAG GAAGGAAGAG AAGCTAAACA AGAAAGAAGA GGAGCTCAAA GTGGCCCAGA 
     1381 CTGACGGGGT CAACGTGGAG ATGCACCTGA AGCAGATTGA AATCAAGAAG TTCAAGTACG 
     1441 GGATTGAAGA GCACGGCAAG GTGAAAATGA GGGGCGGCTT GCTGAGAACC TACATCATCA 
     1501 GCATCCTCTT CAAGTCTGTC TTCGAGGTGG CCTTCCTGCT CATCCAGTGG TACATCTATG 
     1561 GGTTCAGCTT GAGCGCGGTC TACACCTGCA AGAGAGATCC CTGCCCCCAC CAGGTAGACT 
     1621 GCTTCCTCTC ACGTCCCACG GAGAAAACCA TCTTCATCAT CTTCATGCTG GTGGTGTCCT 
     1681 TGGTGTCTCT CGCTTTGAAC ATCATTGAGC TCTTCTACGT CTTCTTCAAA GGCGTTAAGG 
     1741 ATCGCGTGAA GGGAAGAAGC GATCCTTACC ACGCCACCAC TGGCCCACTG AGCCCATCAA 
     1801 AAGACTGCGG ATCTCCAAAA TACGCCTACT TCAATGGCTG CTCCTCACCA ACGGCTCCAC 
     1861 TCTCGCCTAT GTCTCCTCCT GGGTACAAGC TGGTTACTGG TGACAGAAAC AATTCCTCGT 
     1921 GCCGCAATTA CAACAAGCAA GCTAGCGAGC AAAACTGGGC GAACTACAGC GCAGAGCAAA 
     1981 ATCGCATGGG GCAGGCCGGA AGCACCATCT CCAACTCGCA CGCCCAGCCG TTCGATTTCC 
     2041 CCGACGACAA CCAGAATGCC AAAAAAGTTG CTGCTGGACA TGAACTCCAG CCATTAGCCA 
     2101 TCGTGGACCA ACGACCTTCC AGCAGAGCCA GCAGCCGCGC CAGCAGCAGG CCTCGGCCTG 
     2161 ATGACCTGGA GATTGGCCTC GGCCTGATGA CCTGGAGATT ACGCGTACGC GGCCGCTCGA 
     2221 GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA 
     2281 CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT 
     2341 GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC 
     2401 CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT 
     2461 ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT 
     2521 GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA 
     2581 CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG 
     2641 GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT 
     2701 TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC 
     2761 CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT 
     2821 TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC 
     2881 AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC 
     2941 AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG 
     3001 GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA 
     3061 GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC 
     3121 CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA 
     3181 GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG 
     3241 ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT 
     3301 CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT 
     3361 GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG 
     3421 TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC 
     3481 AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC 
     3541 ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA 
     3601 GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC 
     3661 AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG 
     3721 GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA 
     3781 AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT 
     3841 ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT 
     3901 GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG 
     3961 CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT 
     4021 TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG 
     4081 CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG 
     4141 AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG 
     4201 ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG 
     4261 TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA 
     4321 GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA 
     4381 AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT 
     4441 CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT 
     4501 TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT 
     4561 CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT 
     4621 CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC 
     4681 GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA 
     4741 TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT 
     4801 GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA 
     4861 CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA 
     4921 GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG 
     4981 ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA 
     5041 ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC 
     5101 GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG 
     5161 CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA 
     5221 AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG 
     5281 ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA 
     5341 CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC 
     5401 ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC 
     5461 ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA 
     5521 TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC 
     5581 AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG 
     5641 CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC 
     5701 TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC 
     5761 GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG 
     5821 ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG 
     5881 TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG 
     5941 GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT 
     6001 CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
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