LOCUS       RR213190                6647 bp    DNA     circular  SYN 
DEFINITION  Optn (Myc-DDK-tagged ORF) - Rat optineurin (Optn), (10 ug)
ACCESSION   NM_145081
VERSION     NM_145081.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2804..2895 
                     /label="Myc-DDK"  
     misc_feature    2925..3510 
                     /label="PolyA signal"  
     misc_feature    2982..3001 
                     /label="Restriction Enzyme"  
     misc_feature    3786..4458 
                     /label="ColE1"  
     misc_feature    4888..5682 
                     /label="Kan/Neo"  
     misc_feature    5717..6019 
                     /label="SV40 ori"  
     misc_feature    6105..6582 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2786..2792 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2786 
                     /label="Optn"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCCATCAA CCTCTGAGCT GCCTGACTGA GAAGGGGGAC AGCTCCTGTG 
     1081 AGACCCCAGG AAATGGACCC TCCAATATGG TTCACCCCAA CCTGGACACA TTCACTCCTG 
     1141 AGGAGCTGCT GCAGCAAATG AAAGAACTCC TGGTCGAGAA CCACCAGCTG AAAGAAGCCA 
     1201 TGAAGCTAAA TAATCAAGCT ATGAAAGGGC GATTTGAGGA GCTTTCTGCC TGGACAGAGA 
     1261 GGCAGAAGGA AGAACGCCAG TTGTTTGAGA TACAAAGCAA AGAGGCTAAG GAGCGCCTCA 
     1321 AGGCCCTGAG TCATGAAAAT GAGAGGCTGA AGGAAGAGCT TGGAAAACTA AAAGAGAAAT 
     1381 CAGAAAGGCC ATTTGAAGAC ATCACAGGTA GATGCGGGTT TCCCAGAACC GACTTGGAGC 
     1441 AGGAAGTGGG AGCAACTGAA GAGGCAGGTG GAGCAGGAAG TGGAGCATCT GAAGATCCAG 
     1501 GTGAGGCGCC TTCAGGCTTG AGAAAGCGGA CCTTGCTGGG GCATTCGTCT CAGACTGCAG 
     1561 CTCAAGCTCA ACTCCGGCGG CTCCTCAGAA GACTCCTTCG TGGAGACAGG ATGACTGAAG 
     1621 GAGAGGCGGA AGGGGCAATG AAGGAGATGA GGAACAGCGC TGGACCCACA AGGACAGACT 
     1681 CCATCATCAT GGGCAAATGT ACAGAGGACG CCAGGACTTG TGTGGAGTTT GAGGAACTGA 
     1741 CTGTGAGCCA ACTCCTGCTT TGCCTCAGGG AAGGAAACCA AAAGGTGGAG AGACTCGAGA 
     1801 TCGCGCTCAG AGAAGCCAAA GAAAGAATTT CAGATTTTGA AAAGAAAGCA AACGGCCATT 
     1861 CTGCGATTGA AACCCAGACA GAGGGGAGCA CACAAAAAGA AGAGGAGGAC AAAGACCCAG 
     1921 AGAGTGTGGG AATCGAAGTG GAAACTCTGA ACGTTCAAGT GGCCTCTCTG TTTAAGGGGC 
     1981 TTCAAGAGGC GCACACAAAG CTCAGTGAGG CCGAGCTGAT GAAGAAGAGA CTTCAAGAAA 
     2041 AGTGTCAGGC TCTGGAAAGG AAGAACTCTG CAACCCCATC GGAGCTGAAT GAAAAGCAAG 
     2101 AGCTCGTTTA CAGTAACTGG AAGTTAGAGC TGCAGGTGGA GAGCATGCGC TCGGAAATCA 
     2161 AGATGGAGCA GGCCAAGACA GAGGAGGAGA AATCCAGGTT AGCCACTCTT CAGGCAACAC 
     2221 ACGACAAGCT CCTTCAGGAA CACAATAAAG CTCTGAGAAC AATTGAAGAA CTAACCAAAC 
     2281 AACAGGCAGA AAAAGTGGAC AAGGTGCAGC TGCAGGAGCT CAGCGAGAAG CTGGAGCTGG 
     2341 CGGAGCAGGC TCTGGCGTCC AAGCAGCTCC AGATGGATGA GATGAAGCAG ACCATCGCCA 
     2401 AGCAGGAGGA GGACCTGGAG ACCATGGCCG TCCTCAGGGC TCAGATGGAG GTGTACTGTT 
     2461 CAGATTTCCA TGCTGAGAGG GCAGCAAGGG AGAAGATCCA TGAGGAAAAG GAGCAGCTGG 
     2521 CCTTGCAGCT CGCCATTTTG CTGAAAGAGA ACAATGACTT TGAAGATGGA GGCAGTAGGC 
     2581 AGTCCTTGAT GGAAATGCAG TGCCGGCACG GGGCGAGAAC CAGCGACTCT GACCAGCAGG 
     2641 CTTACCTGTT TCAAAGAGGA GCCAAGGACA TGAGCTGGCA GCATGGGCAG CAGCCCCGGA 
     2701 GTATTCCCAT TCACTCGTGC CCCAAGTGTG GGGAGGTCCT GCCGGACATT GACACGCTTC 
     2761 AGATACATGT GATGGACTGT ATCATCGTAT CATCACGCGT ACGCGGCCGC TCGAGCAGAA 
     2821 ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA 
     2881 TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC 
     2941 CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA 
     3001 GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA 
     3061 TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG 
     3121 CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA 
     3181 TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC 
     3241 AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA 
     3301 TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA 
     3361 AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT 
     3421 AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG 
     3481 TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA 
     3541 TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA 
     3601 ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG 
     3661 CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC 
     3721 CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA 
     3781 TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG 
     3841 CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC 
     3901 TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC 
     3961 GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC 
     4021 CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG 
     4081 AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA 
     4141 AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT 
     4201 AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG 
     4261 CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT 
     4321 GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG 
     4381 ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT 
     4441 GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT 
     4501 TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA 
     4561 TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA 
     4621 ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT 
     4681 CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC 
     4741 TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG 
     4801 AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC 
     4861 GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC 
     4921 GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT 
     4981 CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT 
     5041 AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA 
     5101 CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA 
     5161 TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA 
     5221 GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT 
     5281 TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT 
     5341 CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG 
     5401 GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG 
     5461 CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT 
     5521 TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC 
     5581 GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC 
     5641 TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG 
     5701 ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT 
     5761 CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA 
     5821 ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC 
     5881 GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG 
     5941 CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT 
     6001 TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC 
     6061 CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC 
     6121 AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG 
     6181 GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA 
     6241 GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC 
     6301 CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG 
     6361 CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA 
     6421 AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC 
     6481 GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA 
     6541 CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT 
     6601 ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//