LOCUS       RR213130                8481 bp    DNA     circular  SYN 
DEFINITION  Caskin2 (Myc-DDK-tagged ORF) - Rat cask-interacting protein 2 (Caskin2), (10 ug)
ACCESSION   NM_001107065
VERSION     NM_001107065.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4638..4729 
                     /label="Myc-DDK"  
     misc_feature    4759..5344 
                     /label="PolyA signal"  
     misc_feature    4816..4835 
                     /label="Restriction Enzyme"  
     misc_feature    5620..6292 
                     /label="ColE1"  
     misc_feature    6722..7516 
                     /label="Kan/Neo"  
     misc_feature    7551..7853 
                     /label="SV40 ori"  
     misc_feature    7939..8416 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4620..4626 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4620 
                     /label="Caskin2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCCGTGAA CAGGACCTTA TCCTTGCTGT CAAGAATGGA GACGTGACCT 
     1081 GCGTGCAGAA GCTGGTGGCT AAAGTGAAGG CTGCAAAAAC AAAGCTCCTT GGCTCCACGA 
     1141 AGAGACTCAA CATCAACTAC CAGGATGCCG ATGGATTCTC TGCCCTCCAC CATGCTGCCT 
     1201 TGGGGGGCAG CCTGGAGCTC ATAGCCTTGC TGCTGGAGGC TCAAGCCACT GTGGACATCA 
     1261 AGGACAGCAA TGGCATGCGT CCCCTACACT ATGCGGCCTG GCAGGGCCGG CTGGAGCCTG 
     1321 TGAAGCTGCT GCTGAGGGCG TCCGCGGCTG TCAATGCTGC CTCTCTGGAC GGTCAGATCC 
     1381 CTCTGCACCT GGCTGCACAG TATGGGCATT ATGAGGTGTC AGAGATGCTT CTCCAGCACC 
     1441 AGTCTAACCC GTGCCTAGTC AACAAGTTGA AGAAGACACC CCTAGACCTA GCCTGTGAAT 
     1501 TTGGACGGCT CAAGGTGGCC CAGCTGCTTT TGAACAGCCA CTTATGTGTG GCATTGCTGG 
     1561 AGGGAGAGGC AAAGGACCCA TGTGACCCCA ACTACACCAC ACCCCTGCAC TTGGCTGCCA 
     1621 AGAATGGCCA CAGAGAAGTC ATCAGGCAGC TCTTGAAAGC CGGTATTGAG ATCAACCGCC 
     1681 AGACCAAGAC CGGCACTGCC CTCCACGAGG CCGCGCTCTA CGGCAAAACT GAGGTGGTGC 
     1741 GGCTGCTGCT GGAGGGAGGT GTGGATGTGA ATATCCGGAA CACATATAAC CAGACAGCAC 
     1801 TGGACATAGT GAATCAGTTC ACCACCTCCC AGGCCAGCCG GGAAATCAAG CAGCTACTTC 
     1861 GGGAGGCTTC AGGGATCTTG AAGGTTCGAG CGCTTAAGGA TTTCTGGAAT CTTCATGACC 
     1921 CCACTGCTCT CAATATCCGG GCAGGAGATG TCATCACGGT GCTTGAACAG CATCCTGATG 
     1981 GCCGCTGGAA GGGCCACATC CACGAGAGCC AAAGGGGCAC AGACCGTGTT GGCTACTTCC 
     2041 CCCCGGGCAT CGTCGAGGTG GTCAGCAAGC GGGTGGGCAT ACCGGTGACC CGTCTCCCCT 
     2101 CTGCACCCAC CCTGCTGCGG CCAAGCTTCT CCCGGATATC ACAGCCATCG GCTGATGATC 
     2161 CCCTGCCATC TCTCACCTAC GGCCAGCTCC CTCGGGTGGG CCTCAGCCCA GACAGTCCAG 
     2221 CAGGTGACAG GAACAGCGTG GGCAGCGAGG GGAGCGTGGG CAGCATCCGC AGCGCTGGCA 
     2281 GTGGGCAGAG CTCTGAAGGC ACCAATGGCC ATAGCACTGG TCTCCTGATT GAAAATGCTC 
     2341 AGCCACTACC CTCTGCCAAT GAGGACCAGG TACTGCCAGG TCTGCATGCC CCGTCCCCAG 
     2401 CAGACAACCT GAGCCACCGT CCTCTGGCCA GTTACCGCTC TGGGGAGATC TTCACCCAGG 
     2461 ATGTGAGACC AGAACAGCTG CTCGAAGGGA AGGACGCACA GGCCATCCAT AACTGGTTAA 
     2521 GTGAATTCCA GCTAGAGGGC TACACTGCCC ACTTCCTGCA GGCCGGCTAT GACGTGCCAA 
     2581 CCATCAGCCG GATGACACCT GAGGATCTGA CGGCCATTGG AGTGACCAAG CCCGGACACA 
     2641 GGAAGAAGAT CGCCTCAGAG ATTGCTCAGC TCAGCATTGC TGAATGGCTG CCCAACTATA 
     2701 TCCCGGTGGA CTTGTTGGAG TGGCTGTGTG CACTGGGGCT GCCACAGTAC CACAAGCAAC 
     2761 TGGTGAGCAG CGGCTATGAC TCCATGGGGC TGGTGGCCGA TCTCACCTGG GAGGAGCTGC 
     2821 AGGAGATCGG CGTGAACAAG CTAGGCCATC AGAAGAAGCT TATGCTAGGT GTGAAGAGGC 
     2881 TAGCAGAACT TCGGCGGGGC CTACTGCATG GCGAAGCCCT AGGTGAAGGT GGACGCCGGA 
     2941 TGACCAGGGG TCCTGAGCTG ATGGCCATTG AAGGCCTGGA GAACGGGGAC AGTCCAGCTA 
     3001 CGGCCGGCCC TCGCCTCCTC ACCTTTCAGG GCAGTGAGCT GAGCCCAGAG CTACAGGCAG 
     3061 CTATGGCGGG GGGTGGCCCA GAACCACTAC CCCTTCCCCC TGCCCGTTCT CCTAGCCAGG 
     3121 AGAGCATTGG TGCACGCTCC AGGGGATCTG GTCATTCACA GGGACAGCCT GGCTCCCAGC 
     3181 CCAATGCTGG TGAGCCCAGT GCCCCACAGG AGAGGAACCT TCCTGAGGGT ACAGAGCGCC 
     3241 CCTTTAAGCC TTGCTCTCCA CTCCCTGGCC AAGGACCTGC CCCTTATGTC TTTATGTGTC 
     3301 CACAGAGCCT ACCCTCTAGC CCGGCCCCAG CACCACCTCC TGGTGTTCCC CGGGCCTTCT 
     3361 CCTACTTGGC TGGCTCTTCT GCCGCTCCTC CAGACCCACC CCGGCCGAAA CGCCGGTCTC 
     3421 ACAGCCTGAG CCGCCCTGGC CCTGCTGAGG GGGAGGCTGA AGGGGAGGCT GAAGGGCCAG 
     3481 TGGGTAGTGC TTTGGGCAGC TATGCCACAC TGACTCGGAG ACCGGGACGC AGCACCCTTG 
     3541 CCCGGACTAG TCCTAGCCTG ACCCCAACTC GAGGGACTCC CCGAAGCCAG TCCTTTGCCC 
     3601 TCCGTGCCCG TCGTAAAGGT CCCCCGCCCC CGCCCCCCAA GCGCCTCAGT TCCGTCTCTG 
     3661 GCTCCACTGA GCCAACTTCA CTAGAGGGGA CCTCAGGGCC CAAGGAAGGG GCCGCAGGTC 
     3721 CCCGGAGGAG AACACTGAGT GAACCAACCG GCCCCTCAGA GTCCCCTGCT CCTTCTGCGC 
     3781 CAACTGGCCC TGTGTCGGAC ACTGAGGAAG AGGAACCTGT CCTTGAGGGG ACACCGCCTT 
     3841 CTCGGGGCAG CTCAGGGGAA GGGCTGCCAT TTGCAGAGGA AGGGAACCTG ACTATCAAGC 
     3901 AGCGACCGAA ACCAGCCGGT CCCCCTCCCC GTGAGACAAC TGTGCCCCCT GGCCTTGACT 
     3961 TCAATCTCAC AGAGTCAGAC ACGGTCAAAC GCAGGCCCAA ATGCAAGGAG AGAGAGCCAC 
     4021 TACGGACTGC ACTGTTGGCC TTTGGGGTAG TGGGCAGTGA TACCCCTGGC CCCTCCACTC 
     4081 CCCTGTCCTC CCAGGCCCCG TGTGAAGCTC CCTCAGCTCC CTCTAACCCT CCACGGTCTG 
     4141 AGCCCAGCAG CCTTCCATCT CAAGAAGCCC CAGCTCCTCT CAGCCCCGTA ACTCAGCCCC 
     4201 CTGGGCACCC AGGGCCCAGT GCTGGACCAG GTCTGGAAAC CTCAACAGGC AGTAGACAGA 
     4261 ACGTGGACAC GGAGCCTCCA GCTCCCCCCG CTGCCCTCCT CAAAGTGCCT GGAGCAGGAA 
     4321 CAGCTCCCAA GCCTGTGTCT GTGGCCTGCA CTCAGCTGGC ATTTTCTGGC CCAAAGCTGG 
     4381 CTCCCCGGCT CGGCCCTCGC CCAGTTCCCC CTCCAAGGCC TGAGAATACC GGGCCTGTGT 
     4441 GTCCAGGTCG GGCCCAACAG AGACTGGAGC AGACCAGCTC ATCCTTGGAA GCTGCACTGA 
     4501 GGGCGGCTGA GAAGAACATT GGCTCTGAGG AACAAGATGG CCCCACAGGG CCATCCACCA 
     4561 AGCACATTCT GGACGACATC AGTACCATGT TTGATGCCCT GGCTGACCAG TTGGATGCCA 
     4621 TGCTGGACAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     4681 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     4741 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     4801 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     4861 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     4921 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     4981 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5041 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     5101 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     5161 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     5221 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     5281 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     5341 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     5401 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     5461 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     5521 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     5581 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     5641 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     5701 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     5761 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     5821 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     5881 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     5941 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6001 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6061 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     6121 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     6181 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     6241 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     6301 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     6361 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     6421 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     6481 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     6541 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     6601 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     6661 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     6721 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     6781 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     6841 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     6901 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     6961 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7021 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7081 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     7141 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     7201 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     7261 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     7321 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     7381 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     7441 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     7501 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     7561 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     7621 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     7681 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     7741 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     7801 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7861 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     7921 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     7981 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8041 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     8101 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     8161 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     8221 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     8281 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     8341 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     8401 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     8461 CAAAAATTTA ACGCGAATTT T
//