LOCUS       RR213101                8199 bp    DNA     circular  SYN 
DEFINITION  Pold1 (Myc-DDK-tagged ORF) - Rat polymerase (DNA directed), delta 1, catalytic subunit (Pold1), (10 ug)
ACCESSION   NM_021662
VERSION     NM_021662.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4354..4445 
                     /label="Myc-DDK"  
     misc_feature    4475..5060 
                     /label="PolyA signal"  
     misc_feature    4532..4551 
                     /label="Restriction Enzyme"  
     misc_feature    5336..6008 
                     /label="ColE1"  
     misc_feature    6438..7232 
                     /label="Kan/Neo"  
     misc_feature    7267..7569 
                     /label="SV40 ori"  
     misc_feature    7655..8132 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4329..4336 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4329 
                     /label="Pold1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGATGGTAAA CGGCGGCAAG CGCCCAGCTC TGGGGTGCCC CCAAAGCGGG 
     1081 CTTGCAAGGG CCTCTGGGAT GAAGATGAGC CGTCACAGTT TGAGGAGAAC CTGGCGCTGC 
     1141 TGGAGGAGAT AGAGGCCGAG AATCGGCTGC AGGAGGCCGA GGAGGAGCTG CAGCTGCCCC 
     1201 CTGAGGGCAT TGTGGGTGGG CAGTTTTCCA CTGCAGACAT TGACCCACGG TGGCTGCGGC 
     1261 CCACCCCACT TGCCCTGGAC CCCAGCACGG AGCCCCTCAT CTTCCAGCAG CTGGAGATTG 
     1321 ACCACTATGT GGGCACATCA CCTCCCCTGC CAGAAGGACC CCCCGCATCT CGTAACTCAG 
     1381 TGCCCATACT GAGGGCCTTT GGGGTCACCG ATGAGGGCTT CTCCGTCTGC TGCCACATCC 
     1441 ACGGCTTTGC CCCCTACTTC TACACCCCTG CACCTCCGGG TTTTGGGGCT GAGCACCTGA 
     1501 GTGAACTACA GCGGGAGCTG AATGCAGCCA TCAGCCGGGA CCAGCGTGGT GGAAAGGAGC 
     1561 TCTCGGGGCC GGCAGTGCTA GCTATAGAGC TGTGCTCCCG TGAGAGCATG TTTGGGTACC 
     1621 ATGGCCACGG CCCTTCTCCC TTTCTCCGCA TCACCCTGGC ACTACCCCGC CTGATGGCGC 
     1681 CAGCCCGCCG CCTCCTGGAA CAGGGTATCC GAGTGCCAGG CCTGGGCACC CCGAGCTTTG 
     1741 CACCCTATGA AGCCAATGTG GACTTTGAGA TCCGGTTCAT GGTGGATGCT GACATTGTGG 
     1801 GATGCAACTG GTTGGAGCTC CCAGCTGGAA AGTACGTTCG GAGGGCAGAG AAGAAGGCTA 
     1861 CACTGTGTCA GCTGGAGGTG GATGTGCTGT GGTCAGACGT GATCAGTCAC CCACCAGAAG 
     1921 GGCAGTGGCA GCGCATCGCA CCCCTGCGTG TGCTTAGCTT CGACATCGAG TGCGCTGGCC 
     1981 GAAAAGGCAT CTTCCCTGAG CCTGAGCGTG ACCCCGTGAT CCAGATCTGT TCTCTGGGGC 
     2041 TGCGCTGGGG TGAGCCAGAG CCCTTCTTGC GCCTAGCACT CACGCTGCGG CCTTGCGCCC 
     2101 CCATCCTGGG TGCCAAAGTA CAGAGCTATG AACGGGAAGA AGACCTGCTC CAGGCCTGGG 
     2161 CCACTTTCAT CCTCGCCATG GACCCTGACG TGATCACCGG CTACAACATT CAGAACTTTG 
     2221 ACCTCCCCTA CCTCATCTCT CGGGCACAAA CCTTAAAGGT GGACCGATTC CCTTTCCTGG 
     2281 GCCGTGTGAC TGGTCTCCGC TCCAACATCC GTGACTCCTC CTTCCAATCA AGGCAGGTGG 
     2341 GCCGGCGGGA CAGTAAGGTG GTCAGCATGG TGGGTCGCGT TCAGATGGAT ATGCTGCAGG 
     2401 TGCTGCTTCG GGAGTACAAG CTCCGCTCCT ACACGCTCAA CGCTGTGAGC TTCCACTTCC 
     2461 TGGGTGAGCA GAAGGAGGAC GTACAGCACA GCATCATCAC TGACCTACAG AATGGGAATG 
     2521 AACAGACGCG TCGCCGCCTG GCCGTGTACT GCCTGAAGGA TGCCTTTCTG CCTCTTCGCC 
     2581 TACTCGAGCG CCTTATGGTG CTGGTGAACA ATGTGGAGAT GGCGCGTGTC ACTGGTGTAC 
     2641 CCCTTGGGTA CCTGCTCAGC CGAGGCCAGC AGGTCAAGGT CGTGTCTCAG CTGCTGCGCC 
     2701 AGGCCATGCG CGAGGGGCTG CTGATGCCTG TGGTGAAGAC GGAGGGAGGT GAGGACTACA 
     2761 CGGGAGCCAC TGTCATTGAG CCCCTCAAAG GGTACTATGA TGTCCCCATT GCCACCCTGG 
     2821 ACTTCTCCTC TCTGTACCCA TCCATCATGA TGGCCCACAA TCTGTGCTAC ACCACATTGC 
     2881 TACGGCCTGG GGCTGCCCAG AAGTTGGGCC TTAAACCAGA TGAGTTCATC AAGACACCCA 
     2941 CTGGGGATGA GTTTGTGAAG GCATCTGTGC GGAAGGGCCT CCTGCCCCAA ATCCTGGAGA 
     3001 ATCTGCTGAG TGCCCGGAAG AGGGCCAAGG CTGAGCTGGC TCAGGAGACG GACCCCCTGC 
     3061 GGCGACAGGT CTTGGATGGA CGGCAGCTGG CACTAAAAGT GAGTCCCAAC TCTGTGTATG 
     3121 GCTTCACTGG TGCCCAGGTG GGCAAGCTGC CGTGTTTGGA GATCTCCCAG AGTGTCACTG 
     3181 GGTTCGGGCG GCAGATGATT GAGAAAACCA AGCAGCTAGT GGAGACCAAG TACACTCTGG 
     3241 AAAATGGCTA CGATGCTAAT GCCAAGGTGG TCTACGGTGA CACTGACTCT GTGATGTGCC 
     3301 GATTTGGTGT CTCCTCTGTG GCTGAGGCAA TGTCTCTGGG GCGGGAGGCT GCAAACTGGG 
     3361 TATCCAGTCA CTTCCCATCA CCCATCCGGC TGGAGTTCGA GAAGGTTTAC TTTCCCTACC 
     3421 TGCTCATCAG CAAGAAGCGC TATGCCGGCC TACTCTTCTC CTCCCGCTCT GATGCCCATG 
     3481 ACAGAATGGA CTGCAAGGGC CTGGAGGCTG TGCGTAGGGA CAACTGTCCC CTGGTGGCCA 
     3541 ACCTTGTCAC ATCCTCTCTG CGCAGAATCC TCGTGGATCG GGACCCTGAT GGTGCAGTAG 
     3601 CCCATGCAAA GGATGTCATC TCGGACCTGC TGTGCAACCG CATAGACATC TCCCAACTGG 
     3661 TCATCACCAA AGAGTTGACC CGCGCAGCAG CAGACTACGC GGGCAAGCAG GCTCATGTGG 
     3721 AGCTGGCTGA GAGGATGAGG AAGCGTGACC CCGGCAGTGC GCCCAACTTG GGCGACCGAG 
     3781 TACCCTACGT GATCATTGGT GCTGCCAAGG GTGTGGCCGC CTACATGAAG TCGGAGGACC 
     3841 CCCTGTTTGT GCTGGAGCAC AGCCTGCCCA TTGATACTCA GTACTACCTG GAGCAGCAGC 
     3901 TGGCCAAGCC GCTATTGCGC ATCTTTGAGC CCATTCTGGG TGAGGGCCGC GCGGAGTCAG 
     3961 TGCTGCTGCG CGGTGACCAC ACACGCTGCA AAACCGTGCT CACCAGCAAG GTGGGCGGCC 
     4021 TTCTGGCCTT CACCAAGCGC CGAAACTCTT GTATTGGCTG CCGCTCCGTA ATCGACCATC 
     4081 AAGGAGCCGT GTGTAAGTTC TGTCAGCCAC GGGAGTCTGA GCTCTATCAG AAGGAGGTGT 
     4141 CACACCTGAA TGCCCTGGAG GAACGTTTCT CGCGCCTCTG GACACAGTGC CAGCGCTGCC 
     4201 AGGGCAGCTT GCACGAGGAT GTCATCTGTA CCAGCCGCGA CTGTCCCATC TTCTACATGC 
     4261 GCAAGAAGGT GCGCAAGGAC CTGGAGGACC AGGAACGGCT GCTGCAGCGC TTTGGACCTC 
     4321 CTGGCCCTGA GGCCTGGAGC GGACCGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4381 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4441 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4501 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4561 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4621 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4681 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4741 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4801 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4861 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4921 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4981 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5041 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5101 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5161 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5221 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5281 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5341 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5401 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5461 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5521 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5581 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5641 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5701 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5761 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5821 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5881 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5941 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6001 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6061 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6121 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6181 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6241 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6301 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6361 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6421 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6481 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6541 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6601 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6661 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6721 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6781 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6841 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6901 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6961 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7021 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7081 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7141 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7201 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7261 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7321 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7381 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7441 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7501 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7561 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7621 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7681 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7741 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7801 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7861 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7921 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7981 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8041 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8101 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8161 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//