LOCUS       RR213083                9294 bp    DNA     circular  SYN 
DEFINITION  Nup153 (Myc-DDK-tagged ORF) - Rat nucleoporin 153 (Nup153), (10 ug)
ACCESSION   NM_001100470
VERSION     NM_001100470.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5449..5540 
                     /label="Myc-DDK"  
     misc_feature    5570..6155 
                     /label="PolyA signal"  
     misc_feature    5627..5646 
                     /label="Restriction Enzyme"  
     misc_feature    6431..7103 
                     /label="ColE1"  
     misc_feature    7533..8327 
                     /label="Kan/Neo"  
     misc_feature    8362..8664 
                     /label="SV40 ori"  
     misc_feature    8750..9227 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5424..5431 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5424 
                     /label="Nup153"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCCTCAGGA GCCGGAGGCA TAGGAGGGGG CGGCGGCGGC GGCAAGATCC 
     1081 GGACGCGTCG TTGCCACCAG GGGCCGGTGA AGCCTTACCA GCAGGGGCGA CCCCAACACC 
     1141 AGGGCATTCT TAGCAGGGTT ACAGAATCTG TTAAGAATAT TGTACCAGGG TGGCTACAGA 
     1201 GATACTTCAA CAAGAGTGAA AATGCCTGTA GCTGTTCAGT AAATGCAGAT GAAGTTCCAC 
     1261 GTTGGCCGGA AAATAGAGAA GATGAGCGTG AGATTTATGT GGATGAGAAC ACAAACACTG 
     1321 ATGACGGGAG AACCACCCCT GAGCCCACAG GCAGTAACAC AGAAGAACCA TCAACCACCA 
     1381 GTACTGCTTC AAATTATCCA GATGTATTAA CAAGACCTTC TCTTCATCGG AGTCATCTGA 
     1441 ATTTCTCCGT GTTAGAATCC CCTGCTTTGC ACTGTCAGCC ATCCACGTCC TCTGCATTCC 
     1501 CAATCGGCAG TTCTGGATTT TCCCTTGTGA AGGAGATTAA AGATTCTACC TCTCAGCATG 
     1561 ATGATGATAA CATCTCAACT ACCAGTGGGT TCTCTTCAAG AGCTTCTGAG AAAGATATAG 
     1621 CTGTTTCAAA GAACACTTCC CTGCCACCTT TGTGGTCCCC CGAAGCAGAG CGTTCTCATT 
     1681 CACTCTCACA ACATACTGCC ATCAGCTCCA AAAAACCAGC ATTCAACTTG TCTGCCTTTG 
     1741 GAACACTTTC CACCTCACTT GGGAATTCTT CAATTCTTAA AACAAGTCAA CTTGGAGACT 
     1801 CTCCTTTTTA TCCTGGCAAA ACAACATATG GTGGGGCAGC TGCTGCTGTA AGACAGAATA 
     1861 AAGTACGAAG TACACCTTAC CAGGCTCCAG TTCGAAGACA GATGAAAGCC AAGCAGCTCA 
     1921 ATGCACAGTC TTATGGTGTG ACCAGTTCAA CAGCACGGCG GATATTGCAG TCATTAGAAA 
     1981 AGATGTCAAG CCCTCTTGCG GATGCAAAAA GAATTCCATC TGCTGTTTCT TCTCCTCTGA 
     2041 ATTCTCCCCT TGATAGGAGT GGGATAGATA GCACAGTTTT TCAGGCAAAA AAGGAAAAGG 
     2101 TGGACTCTCA GTATCCCCCT GTTCAGAGAC TAATGACCCC AAAGCCAGTT TCCATAGCAA 
     2161 CAAATAGAAC TGTCTATTTT AAACCATCTC TGACCCCATC TGGTGACTTA AGGAAGACTA 
     2221 ATCAAAGGAT AGATAAAAAG AACAGTACTG TAGATGAAAA AAATATCTCA AGACAAAATA 
     2281 GAGAGCAAGA AAGTGGCTTT TCATACCCCA ATTTCAGTAT TCCTGCAGCC AATGGTTTAT 
     2341 CTTCTGGAGT AGGTGGTGGA GGTGGCAAGA TGAGAAGAGA GAGAACAACA CATTTTGTTG 
     2401 CTTCTAAACC TTCAGAGGAG GAGGAAGTAG AAGTACCATT GTTACCGCAA ATCTCTCTAC 
     2461 CAATCAGCAG TTCTTCACTG CCCACCTTCA ACTTTAGTTC CCCTGCGATC TCGGCTGCTT 
     2521 CTTCGTCATC AGTCAGCCCT TCTCAGCCAT TATCAAACAA GGTACAGATG ACCTCTCTGG 
     2581 GCAGCACAGG CAATCCCGTG TTCACCTTTT CCTCTCCCAT TGTGAAGTCC ACGCAGGCAG 
     2641 ACGTGCTACC TCCAGCATCT ATTGGATTTA CGTTTAGTGT GCCTCTTGCA AAAACAGAAC 
     2701 TTTCTGGCCC TAATAGTTCT TCAGAAACAG TTTTAAGTAG TTCAGTTACA GCTCAAGATA 
     2761 ACACTGTAGT GAATAGTTCA AGCTCTAAGA AGCGAAGTGC CCCTTGTGAG GACCCTTTTA 
     2821 CACCTGCAAA GATCCTGAGA GAAGGAAGTG TTTTAGACAT TCTGAAAACG CCTGGTTTCA 
     2881 TGTCACCAAA GGTTGATTCT CCTGCTCTTC AGCCCACAAC TACAAGCTCA ATAGTTTATA 
     2941 CAAGACCAGC AATAAGTACT TTTTCTTCTA GTGGGGTTGA GTTTGGAGAA AGTTTAAAAG 
     3001 CTGGATCATC ATGGCAGTGT GACACATGTC TACTCCAGAA CAAAGTCACA GACAATAAGT 
     3061 GTATAGCCTG TCAAGCAGCA AAACTGCCAC TGAAAGAAAC TGCTAAACAG ACTGGAATTG 
     3121 GGACACCAAG CAAAAGTGAC AAGCCAGCTT CTACATCAGG GACAGGTTTT GGAGATAAAT 
     3181 TTAAACCAGC AATAGGAACT TGGGATTGTG ATACCTGTTT AGTGCAAAAT AAGCCTGAAG 
     3241 CAGTAAAATG TGTAGCCTGT GAAACACCCA AACCTGGCAC TGGTGTGAAG CGAGCCCTTC 
     3301 CATTGACGGT GGCTTCAGAA AGTCCTGTGA CTGCTTCCTC TTCTACCACC GTGACCACTG 
     3361 GTACACTAGG ATTTGGAGAT AAATTCAAAA GGCCCGTGGG ATCTTGGGAG TGTCCGGTAT 
     3421 GCTGTGTTTC TAATAAGGCA GAAGACAGCA GATGTGTCTC CTGTACATCT GAGAAACCAG 
     3481 GTTTGGTATC TGCCTCAAGC AGCAACCCTG TCCCTGTCTC TCTGCCTTCT GGAGGCTGTT 
     3541 TAGGATTGGA CAAGTTTAAA AAACCAGAGG GAAGCTGGGA CTGTGAAGTA TGCCTAGTGC 
     3601 AAAATAAAGC GGACTCTACC AAGTGCATAG CTTGTGAAAG TGCAAAGCCA GGCACAAAGT 
     3661 CTGAGTTTAA AGGCTTTGGG ACATCTTCAT CTTTAAATCC AGCACCCTCA GCCTTCAAAT 
     3721 TTGGTATTCC ATCATCATCT TCTGGGCTTT CTCAAACTTT TACAAGCACT GGAAATTTTA 
     3781 AATTTGGAGA TCAGGGAGGA TTCAAATTAG GCACTTCATC TGACTCTGGG TCTACAAACA 
     3841 CCATGAATAC AAACTTTAAA TTTCCTAAAC CAACTGGAGA TTTTAAATTT GGAGTATTAC 
     3901 CTGATTCCAA ACCTGAAGAA ATAAAAAACG ACAGCAAGAA TGATAATTTT CAGTTTGGAC 
     3961 CTTCTTCTGG CTTAAGCAAT CCAGCGTCTT CAGCTCCATT TCAGTTTGGG GTATCTACTC 
     4021 TTGGGCAGCA GGAAAAGAAA GAGGAGCTGC CTCAGTCTTC ATCTGCAGGC TTCAGTTTTG 
     4081 GTGCAGGTGT TGCTAACCCC TCTAGTGCTG CTATTGACAC CACAGTGACC TCAGAGAACA 
     4141 AGAGCGGCTT CAACTTTGGA ACCATAGACA CTAAGAGTGT CTCAGTGACT CCTTTCACGT 
     4201 ACAAGACAAC AGAAGCAAAG AAAGAAGACG CGTCTGCTAC CAAGGGTGGG TTCACATTTG 
     4261 GTAAAGTGGA CTCTGCTGCT TTGTCATCTC CCTCAATGTT TGTGTTGGGA AGGACAGAGG 
     4321 AGAAACAGCA AGAACCTGTT ACTTCTACTT CTCTGGTATT TGGAAAGAAA GCTGACAATG 
     4381 AGGAGCCAAA GTGTCAACCA GTATTTTCCT TTGGAAATTC AGAGCAAACC AAAGACGAGA 
     4441 GTTCCTCCAA GCCGACCTTC AGTTTCAGTG TGGCAAAACC ATCTGTGAAG GAGTCTGATC 
     4501 AGCTGGCCAA GGCTACTTTT GCATTTGGAA ATCAAACCAA TACAACAACT GATCAAGGTG 
     4561 CAGCAAAGCC AGCTTTTAGC TTCTTGAACA GCAGTTCCTC TAGTTCAAGT ACGCCGGCCA 
     4621 CTTCATCCAG TGCTAGCATA TTTGGTAGTT CCACCTCTTC CTCCAGCCCG CCTGTGGCAG 
     4681 CCTTTGTGTT TGGACAGGCC AGTAATCCTG TCAGCAGCTC TGCCTTCGGA AACTCCGCCG 
     4741 AGTCCAGTAC ATCTCAGCCT TTGCTCTTTC CTCAAGATGG CAAACCAGCC ACCACGTCCA 
     4801 GCACTGCCTC AGCTGCCCCT CCATTTGTAT TTGGAACAGG AGCCAGTAGT AATAGTACTG 
     4861 TATCTTCTGG GTTCACTTTT GGGGCTACAA CCACATCAAG CTCTTCAGGA TCCTTCTTTG 
     4921 TATTTGGCAC TGGACATTCA GCACCATCTG CCAGTCCAGC ATTTGGTGCT AACCAGACTC 
     4981 CAACATTTGG ACAAAGTCAA GGTGCCAGCC AGCCTAACCC TCCAAGCTTT GGCTCTATAT 
     5041 CGTCTTCAAC AGCATTATTT TCTGCTGGTT CTCAGCCTGT ACCACCACCT ACATTTGGGA 
     5101 CAGTGTCAAG CAGCAGCCAA CCTCCTGTGT TTGGACAGCA GCCTAGTCAG TCTGCATTTG 
     5161 GCTCTGGGAC AGCCAATGCT AGTTCGGTTT TCCAGTTTGG CAGCAGCACT ACAAATTTCA 
     5221 ACTTCACAAA CAATAATCCA TCAGGAGTGT TCACATTTGG TGCAAGTCCC AGCACGCCTG 
     5281 CAGCCGCAGC CCAGCCCTCA GGTTCGGGGG GCTTTTCATT CAGCCAGTCT CCAGCATCAT 
     5341 TTACAGTGGG GTCAAATGGG AAAAATATGT TCTCTTCTTC CGGAACTTCA GTTTCTGGTC 
     5401 GCAAGATAAA GACTGCTGTT AGACGCAAGA AAAGCGGACC GACGCGTACG CGGCCGCTCG 
     5461 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     5521 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     5581 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     5641 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     5701 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     5761 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     5821 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     5881 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     5941 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     6001 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     6061 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     6121 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     6181 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     6241 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     6301 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     6361 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     6421 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     6481 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     6541 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     6601 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     6661 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     6721 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     6781 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     6841 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     6901 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     6961 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     7021 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     7081 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     7141 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     7201 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     7261 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     7321 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     7381 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     7441 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     7501 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     7561 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     7621 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     7681 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     7741 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     7801 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     7861 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     7921 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     7981 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     8041 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     8101 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     8161 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     8221 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     8281 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     8341 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     8401 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     8461 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     8521 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     8581 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     8641 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     8701 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     8761 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     8821 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     8881 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     8941 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     9001 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     9061 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     9121 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     9181 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     9241 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//