LOCUS       RR213062                7079 bp    DNA     circular  SYN 
DEFINITION  Hgf (Myc-DDK-tagged ORF) - Rat hepatocyte growth factor (Hgf), (10 ug)
ACCESSION   NM_017017
VERSION     NM_017017.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3236..3327 
                     /label="Myc-DDK"  
     misc_feature    3357..3942 
                     /label="PolyA signal"  
     misc_feature    3414..3433 
                     /label="Restriction Enzyme"  
     misc_feature    4218..4890 
                     /label="ColE1"  
     misc_feature    5320..6114 
                     /label="Kan/Neo"  
     misc_feature    6149..6451 
                     /label="SV40 ori"  
     misc_feature    6537..7014 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3218..3224 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3218 
                     /label="Hgf"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GATGTGGGGG ACCAAACTTC TGCCGGTCCT GTTGCTGCAG CATGTCCTGC 
     1081 TGCACCTCCT CCTGCTTCCT GTCACCATCC CCTATGCAGA AGGACAGAAG AAGAGAAGAA 
     1141 ATACTCTTCA TGAATTCAAA AAGTCAGCAA AAACTACTCT TACCAAGGAA GACCCATTAG 
     1201 TGAAGATTAA AACCAAAAAA GTGAACTCTG CAGATGAGTG TGCCAACAGG TGCATCAGAA 
     1261 ACAAGGGCTT TCCATTCACT TGCAAGGCCT TTGTTTTTGA TAAGTCGAGA AAACGATGCT 
     1321 ACTGGTATCC TTTCAATAGT ATGTCAAGTG GAGTGAAAAA AGGGTTTGGC CATGAATTTG 
     1381 ACCTCTATGA AAACAAAGAC TATATTAGAA ATTGCATCAT TGGTAAAGGA GGCAGCTATA 
     1441 AGGGGACAGT ATCCATCACT AAGAGTGGCA TCAAGTGCCA GCCTTGGAAT TCCATGATCC 
     1501 CCCATGAACA CAGCTTTTTG CCTTCGAGCT ATCGCGGTAA AGACCTACAG GAAAACTACT 
     1561 GTCGAAATCC TCGAGGGGAA GAAGGGGGAC CCTGGTGTTT CACAAGCAAT CCAGAGGTAC 
     1621 GCTACGAAGT CTGTGACATT CCTCAGTGTT CAGAAGTTGA ATGCATGACC TGCAACGGTG 
     1681 AAAGCTACAG AGGTCCCATG GATCACACAG AATCAGGCAA GACATGTCAG CGCTGGGATC 
     1741 AGCAGACACC ACACCGGCAC AAATTCTTGC CGGAAAGATA TCCCGACAAG GGCTTTGATG 
     1801 ATAATTATTG CCGCAATCCC GATGGCAAGC CGAGGCCATG GTGCTACACT CTTGACCCTG 
     1861 ACACCCCTTG GGAGTATTGT GCAATTAAAA TGTGCGCTCA CAGTGCTGTG AATGAGACTG 
     1921 ATGTTCCCAT GGAAACAACT GAATGTATAA AAGGCCAAGG AGAAGGTTAC AGGGGAACCA 
     1981 CCAATACCAT TTGGAATGGA ATTCCGTGTC AGCGTTGGGA TTCGCAGTAC CCCCACAAGC 
     2041 ATGACATCAC TCCCGAGAAC TTCAAATGCA AGGACCTTAG AGAAAATTAT TGCCGCAATC 
     2101 CGGATGGGGC TGAATCACCA TGGTGTTTTA CCACTGATCC AAACATCCGA GTTGGTTACT 
     2161 GCTCTCAAAT TCCCAAATGT GACGTGTCAA GTGGACAAGA TTGTTATCGT GGCAATGGGA 
     2221 AAAACTACAT GGGCAACTTA TCCAAAACAA GGTCTGGACT CACATGTTCC ATGTGGGACA 
     2281 AGAATATGGA GGATTTACAC CGTCATATCT TCTGGGAGCC AGACGCTAGC AAGTTGACTA 
     2341 AGAATTACTG CCGGAACCCC GATGACGACG CCCATGGACC TTGGTGCTAC ACAGGGAATC 
     2401 CTCTCGTTCC TTGGGATTAT TGCCCTATTT CCCGTTGTGA AGGAGATACT ACACCTACAA 
     2461 TTGTCAATTT GGACCATCCT GTAATATCCT GTGCCAAAAC AAAACAACTG CGAGTTGTAA 
     2521 ATGGCATTCC AACACAAACA ACAGTAGGGT GGATGGTTAG TTTGAAATAC AGGAATAAAC 
     2581 ACATCTGTGG GGGATCATTG ATAAAGGAAA GTTGGGTTCT TACTGCAAGG CAATGTTTTC 
     2641 CAGCTAGAAA CAAAGACTTG AAAGACTATG AAGCTTGGCT TGGAATCCAT GATGTCCATG 
     2701 AGAGAGGCGA GGAGAAACGC AAACAGATCT TAAACATTTC CCAGCTAGTC TATGGACCTG 
     2761 AAGGCTCAGA TTTGGTTTTA CTGAAGCTTG CTCGCCCTGC AATCCTGGAT AACTTTGTCA 
     2821 GTACAATTGA TTTACCTAGT TATGGCTGTA CAATCCCTGA AAAGACTACT TGCAGTATTT 
     2881 ACGGCTGGGG CTACACTGGA TTGATCAACG CAGATGGTTT ATTACGAGTA GCTCATCTGT 
     2941 ATATTATGGG GAATGAGAAA TGCAGTCAGC ACCATCAAGG CAAGGTGACT TTGAATGAGT 
     3001 CTGAATTATG TGCTGGGGCT GAAAAGATTG GATCAGGACC TTGTGAGGGA GATTATGGTG 
     3061 GCCCACTCAT TTGTGAACAA CACAAAATGA GAATGGTTCT TGGTGTCATT GTTCCTGGTC 
     3121 GTGGATGTGC CATCCCAAAT CGTCCTGGTA TTTTTGTTCG AGTAGCATAT TATGCAAAAT 
     3181 GGATACACAA AGTAATTTTG ACATACAAGT TGTGACATAC AAGTTGACGC GTACGCGGCC 
     3241 GCTCGAGCAG AAACTCATCT CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA 
     3301 GGATGACGAC GATAAGGTTT AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC 
     3361 CCGGGTGGCA TCCCTGTGAC CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC 
     3421 CAGTGCCCAC CAGCCTTGTC CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT 
     3481 CCTTCTATAA TATTATGGGG TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA 
     3541 CAACCTGTAG GGCCTGCGGG GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT 
     3601 GGCTCACTGC AATCTCCGCC TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT 
     3661 TGTTGGGATT CCAGGCATGC ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC 
     3721 GGGGTTTCAC CATATTGGCC AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC 
     3781 CTTGGCCTCC CAAATTGCTG GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC 
     3841 TGATTTTAAA ATAACTATAC CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC 
     3901 ATGCCCAACC GGTGGGACAT TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT 
     3961 CCACTCAGTA GATGCCTGTT GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC 
     4021 TCAAAGGCGG TAATACGGTT ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA 
     4081 GCAAAAGGCC AGCAAAAGGC CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT 
     4141 AGGCTCCGCC CCCCTGACGA GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC 
     4201 CCGACAGGAC TATAAAGATA CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT 
     4261 GTTCCGACCC TGCCGCTTAC CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG 
     4321 CTTTCTCATA GCTCACGCTG TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG 
     4381 GGCTGTGTGC ACGAACCCCC CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT 
     4441 CTTGAGTCCA ACCCGGTAAG ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG 
     4501 ATTAGCAGAG CGAGGTATGT AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC 
     4561 GGCTACACTA GAAGAACAGT ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA 
     4621 AAAAGAGTTG GTAGCTCTTG ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT 
     4681 GTTTGCAAGC AGCAGATTAC GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT 
     4741 TCTACGGGGT CTGACGCTCA GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA 
     4801 TTATCAAAAA GGATCTTCAC CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC 
     4861 TAAAGTATAT ATGAGTAACC TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG 
     4921 AGCCCTGGGC CTTCACCCGA ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG 
     4981 TATTGGCCCC AATGGGGTCT CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC 
     5041 CCGCGTTTAT GAACAAACGA CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC 
     5101 ATAGCGCGGG TTCCTTCCGG TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG 
     5161 TGGGCGAAGA ACTCCAGCAT GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG 
     5221 AAAACGATTC CGAAGCCCAA CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT 
     5281 GGCAGGTTGG GCGTCGCTTG GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT 
     5341 CGTCAAGAAG GCGATAGAAG GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA 
     5401 CGAGGAAGCG GTCAGCCCAT TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG 
     5461 CTATGTCCTG ATAGCGATCC GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC 
     5521 GGCCATTTTC CACCATGATA TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT 
     5581 CGCCGTCGGG CATGCTCGCC TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT 
     5641 GCTCTTCGTC CAGATCATCC TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT 
     5701 CGATGCGATG TTTCGCTTGG TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC 
     5761 GCCGCATTGC ATCAGCCATG ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA 
     5821 GATCCTGCCC CGGCACTTCG CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT 
     5881 CGAGCACAGC TGCGCAAGGA ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT 
     5941 CTTGCAGTTC ATTCAGGGCA CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT 
     6001 GCGCTGACAG CCGGAACACG GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT 
     6061 AGCCGAATAG CCTCTCCACC CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA 
     6121 TCATGCGAAA CGATCCTCAT CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA 
     6181 AAAAAGCCTC CTCACTACTT CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA 
     6241 TAAATAAAAA AAATTAGTCA GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG 
     6301 GGCGGGATGG GCGGAGTTAG GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT 
     6361 TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG 
     6421 AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC 
     6481 TGACACACAT TCCACAGCTG GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT 
     6541 GAAGCATTTA TCAGGGTTAT TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA 
     6601 ATAAACAAAT AGGGGTTCCG CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA 
     6661 GCGGCGCATT AAGCGCGGCG GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA 
     6721 GCGCCCTAGC GCCCGCTCCT TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT 
     6781 TTCCCCGTCA AGCTCTAAAT CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC 
     6841 ACCTCGACCC CAAAAAACTT GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT 
     6901 AGACGGTTTT TCGCCCTTTG ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC 
     6961 AAACTGGAAC AACACTCAAC CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC 
     7021 CGATTTCGGC CTATTGGTTA AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//