LOCUS       RR212955                6660 bp    DNA     circular  SYN 
DEFINITION  Gad1 (Myc-DDK-tagged ORF) - Rat glutamate decarboxylase 1 (Gad1), (10 ug)
ACCESSION   NM_017007
VERSION     NM_017007.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2817..2908 
                     /label="Myc-DDK"  
     misc_feature    2938..3523 
                     /label="PolyA signal"  
     misc_feature    2995..3014 
                     /label="Restriction Enzyme"  
     misc_feature    3799..4471 
                     /label="ColE1"  
     misc_feature    4901..5695 
                     /label="Kan/Neo"  
     misc_feature    5730..6032 
                     /label="SV40 ori"  
     misc_feature    6118..6595 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2799..2805 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2799 
                     /label="Gad1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCATCTTCC ACGCCTTCGC CTGCAACCTC CTCGAACGCG GGAGCGGATC 
     1081 CTAATACTAC CAACCTGCGT CCTACAACAT ATGATACTTG GTGTGGCGTA GCCCATGGAT 
     1141 GCACCAGAAA ACTGGGCCTG AAGATCTGTG GTTTCTTGCA AAGGACCAAT AGCCTGGAAG 
     1201 AGAAGAGTCG TCTTGTGAGC GCCTTCAGGG AGAGGCAGGC CTCCAAGAAC CTGCTTTCCT 
     1261 GTGAAAACAG TGACCCTGGT GCCCGCTTCC GGCGCACAGA GACGGACTTC TCCAACCTGT 
     1321 TTGCTCAAGA TCTGCTTCCA GCTAAGAACG GGGAGGAGCA AACTGTGCAG TTCTTACTGG 
     1381 AGGTGGTTGA CATACTCCTC AACTATGTCC GCAAGACGTT TGATCGCTCC ACCAAGGTTT 
     1441 TGGACTTCCA CCACCCACAC CAGTTGCTGG AAGGCATGGA AGGTTTTAAT TTGGAGCTGT 
     1501 CTGACCACCC CGAGTCTCTG GAGCAGATCC TGGTTGACTG TAGAGACACC CTAAAGTACG 
     1561 GGGTTCGCAC AGGTCACCCT CGGTTTTTCA ACCAGCTCTC TACTGGTTTG GATATCATTG 
     1621 GTTTAGCTGG CGAATGGCTG ACATCAACTG CCAATACCAA TATGTTCACA TATGAAATTG 
     1681 CACCCGTGTT TGTTCTTATG GAACAGATCA CACTTAAGAA GATGCGAGAG ATCATTGGAT 
     1741 GGTCAAATAA AGATGGTGAT GGGATATTTT CTCCTGGGGG AGCCATATCC AACATGTACA 
     1801 GCATCATGGC GGCTCGGTAC AAGTACTTTC CAGAAGTGAA AACAAAAGGC ATGGCGGCTG 
     1861 TGCCCAAACT GGTCCTCTTC ACCTCAGAAC ACAGTCACTA TTCCATAAAG AAGGCTGGGG 
     1921 CTGCGCTTGG CTTTGGAACT GACAATGTGA TCCTGATAAA GTGCAATGAA AGGGGGAAGA 
     1981 TAATTCCAGC TGATTTAGAG GCAAAAATTC TTGATGCCAA ACAAAAGGGA TTTGTTCCCC 
     2041 TTTATGTCAA TGCAACCGCA GGCACGACTG TTTATGGAGC GTTTGATCCG ATCCAGGAGA 
     2101 TTGCAGACAT ATGTGAGAAA TACAACCTTT GGCTGCATGT GGATGCTGCC TGGGGTGGTG 
     2161 GGCTGCTCAT GTCCCGGAAG CATCGCCACA AACTCAGCGG CATAGAAAGG GCCAATTCAG 
     2221 TCACCTGGAA CCCTCACAAG ATGATGGGTG TGCTGCTCCA GTGTTCTGCC ATCCTGGTCA 
     2281 AGGAAAAGGG TATACTCCAA GGATGCAACC AGATGTGTGC AGGCTACCTC TTCCAGCCAG 
     2341 ACAAGCAGTA TGACGTCTCC TACGATACAG GGGACAAGGC GATTCAGTGT GGCCGCCATG 
     2401 TGGACATCTT CAAGTTCTGG CTGATGTGGA AAGCAAAGGG CACTGTGGGA TTTGAAAATC 
     2461 AGATCAACAA ATGCCTGGAG CTGGCTGAAT ACCTCTATGC CAAGATTAAA AACAGAGAAG 
     2521 AGTTTGAGAT GGTTTTCAAT GGTGAGCCTG AGCACACAAA TGTCTGTTTC TGGTACATAC 
     2581 CACAAAGCCT CCGAGGGGTT CCAGACAGCC CTGAGCGACG AGAAAAACTA CACAGGGTGG 
     2641 CTCCCAAGAT CAAAGCCCTG ATGATGGAGT CAGGAACAAC CATGGTTGGT TACCAGCCTC 
     2701 AAGGGGACAA GGCCAACTTC TTCCGGATGG TCATCTCCAA CCCAGCCGCC ACCCAGTCTG 
     2761 ACATCGATTT CCTCATCGAG GAAATAGAGA GGTTGGGTCA AGATCTGACG CGTACGCGGC 
     2821 CGCTCGAGCA GAAACTCATC TCAGAAGAGG ATCTGGCAGC AAATGATATC CTGGATTACA 
     2881 AGGATGACGA CGATAAGGTT TAAACGGCCG GCCGCGGTCA TAGCTGTTTC CTGAACAGAT 
     2941 CCCGGGTGGC ATCCCTGTGA CCCCTCCCCA GTGCCTCTCC TGGCCCTGGA AGTTGCCACT 
     3001 CCAGTGCCCA CCAGCCTTGT CCTAATAAAA TTAAGTTGCA TCATTTTGTC TGACTAGGTG 
     3061 TCCTTCTATA ATATTATGGG GTGGAGGGGG GTGGTATGGA GCAAGGGGCA AGTTGGGAAG 
     3121 ACAACCTGTA GGGCCTGCGG GGTCTATTGG GAACCAAGCT GGAGTGCAGT GGCACAATCT 
     3181 TGGCTCACTG CAATCTCCGC CTCCTGGGTT CAAGCGATTC TCCTGCCTCA GCCTCCCGAG 
     3241 TTGTTGGGAT TCCAGGCATG CATGACCAGG CTCAGCTAAT TTTTGTTTTT TTGGTAGAGA 
     3301 CGGGGTTTCA CCATATTGGC CAGGCTGGTC TCCAACTCCT AATCTCAGGT GATCTACCCA 
     3361 CCTTGGCCTC CCAAATTGCT GGGATTACAG GCGTGAACCA CTGCTCCCTT CCCTGTCCTT 
     3421 CTGATTTTAA AATAACTATA CCAGCAGGAG GACGTCCAGA CACAGCATAG GCTACCTGGC 
     3481 CATGCCCAAC CGGTGGGACA TTTGAGTTGC TTGCTTGGCA CTGTCCTCTC ATGCGTTGGG 
     3541 TCCACTCAGT AGATGCCTGT TGAATTGGGT ACGCGGCCAG CGGCGAGCGG TATCAGCTCA 
     3601 CTCAAAGGCG GTAATACGGT TATCCACAGA ATCAGGGGAT AACGCAGGAA AGAACATGTG 
     3661 AGCAAAAGGC CAGCAAAAGG CCAGGAACCG TAAAAAGGCC GCGTTGCTGG CGTTTTTCCA 
     3721 TAGGCTCCGC CCCCCTGACG AGCATCACAA AAATCGACGC TCAAGTCAGA GGTGGCGAAA 
     3781 CCCGACAGGA CTATAAAGAT ACCAGGCGTT TCCCCCTGGA AGCTCCCTCG TGCGCTCTCC 
     3841 TGTTCCGACC CTGCCGCTTA CCGGATACCT GTCCGCCTTT CTCCCTTCGG GAAGCGTGGC 
     3901 GCTTTCTCAT AGCTCACGCT GTAGGTATCT CAGTTCGGTG TAGGTCGTTC GCTCCAAGCT 
     3961 GGGCTGTGTG CACGAACCCC CCGTTCAGCC CGACCGCTGC GCCTTATCCG GTAACTATCG 
     4021 TCTTGAGTCC AACCCGGTAA GACACGACTT ATCGCCACTG GCAGCAGCCA CTGGTAACAG 
     4081 GATTAGCAGA GCGAGGTATG TAGGCGGTGC TACAGAGTTC TTGAAGTGGT GGCCTAACTA 
     4141 CGGCTACACT AGAAGAACAG TATTTGGTAT CTGCGCTCTG CTGAAGCCAG TTACCTTCGG 
     4201 AAAAAGAGTT GGTAGCTCTT GATCCGGCAA ACAAACCACC GCTGGTAGCG GTGGTTTTTT 
     4261 TGTTTGCAAG CAGCAGATTA CGCGCAGAAA AAAAGGATCT CAAGAAGATC CTTTGATCTT 
     4321 TTCTACGGGG TCTGACGCTC AGTGGAACGA AAACTCACGT TAAGGGATTT TGGTCATGAG 
     4381 ATTATCAAAA AGGATCTTCA CCTAGATCCT TTTAAATTAA AAATGAAGTT TTAAATCAAT 
     4441 CTAAAGTATA TATGAGTAAC CTGAGGCTAT GGCAGGGCCT GCCGCCCCGA CGTTGGCTGC 
     4501 GAGCCCTGGG CCTTCACCCG AACTTGGGGG GTGGGGTGGG GAAAAGGAAG AAACGCGGGC 
     4561 GTATTGGCCC CAATGGGGTC TCGGTGGGGT ATCGACAGAG TGCCAGCCCT GGGACCGAAC 
     4621 CCCGCGTTTA TGAACAAACG ACCCAACACC GTGCGTTTTA TTCTGTCTTT TTATTGCCGT 
     4681 CATAGCGCGG GTTCCTTCCG GTATTGTCTC CTTCCGTGTT TCAGTTAGCC TCCCCCTAGG 
     4741 GTGGGCGAAG AACTCCAGCA TGAGATCCCC GCGCTGGAGG ATCATCCAGC CGGCGTCCCG 
     4801 GAAAACGATT CCGAAGCCCA ACCTTTCATA GAAGGCGGCG GTGGAATCGA AATCTCGTGA 
     4861 TGGCAGGTTG GGCGTCGCTT GGTCGGTCAT TTCGAACCCC AGAGTCCCGC TCAGAAGAAC 
     4921 TCGTCAAGAA GGCGATAGAA GGCGATGCGC TGCGAATCGG GAGCGGCGAT ACCGTAAAGC 
     4981 ACGAGGAAGC GGTCAGCCCA TTCGCCGCCA AGCTCTTCAG CAATATCACG GGTAGCCAAC 
     5041 GCTATGTCCT GATAGCGATC CGCCACACCC AGCCGGCCAC AGTCGATGAA TCCAGAAAAG 
     5101 CGGCCATTTT CCACCATGAT ATTCGGCAAG CAGGCATCGC CATGGGTCAC GACGAGATCC 
     5161 TCGCCGTCGG GCATGCTCGC CTTGAGCCTG GCGAACAGTT CGGCTGGCGC GAGCCCCTGA 
     5221 TGCTCTTCGT CCAGATCATC CTGATCGACA AGACCGGCTT CCATCCGAGT ACGTGCTCGC 
     5281 TCGATGCGAT GTTTCGCTTG GTGGTCGAAT GGGCAGGTAG CCGGATCAAG CGTATGCAGC 
     5341 CGCCGCATTG CATCAGCCAT GATGGATACT TTCTCGGCAG GAGCAAGGTG AGATGACAGG 
     5401 AGATCCTGCC CCGGCACTTC GCCCAATAGC AGCCAGTCCC TTCCCGCTTC AGTGACAACG 
     5461 TCGAGCACAG CTGCGCAAGG AACGCCCGTC GTGGCCAGCC ACGATAGCCG CGCTGCCTCG 
     5521 TCTTGCAGTT CATTCAGGGC ACCGGACAGG TCGGTCTTGA CAAAAAGAAC CGGGCGCCCC 
     5581 TGCGCTGACA GCCGGAACAC GGCGGCATCA GAGCAGCCGA TTGTCTGTTG TGCCCAGTCA 
     5641 TAGCCGAATA GCCTCTCCAC CCAAGCGGCC GGAGAACCTG CGTGCAATCC ATCTTGTTCA 
     5701 ATCATGCGAA ACGATCCTCA TCCTGTCTCT TGATCGATCT TTGCAAAAGC CTAGGCCTCC 
     5761 AAAAAAGCCT CCTCACTACT TCTGGAATAG CTCAGAGGCC GAGGCGGCCT CGGCCTCTGC 
     5821 ATAAATAAAA AAAATTAGTC AGCCATGGGG CGGAGAATGG GCGGAACTGG GCGGAGTTAG 
     5881 GGGCGGGATG GGCGGAGTTA GGGGCGGGAC TATGGTTGCT GACTAATTGA GATGCATGCT 
     5941 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCTGGTTG CTGACTAATT 
     6001 GAGATGCATG CTTTGCATAC TTCTGCCTGC TGGGGAGCCT GGGGACTTTC CACACCCTAA 
     6061 CTGACACACA TTCCACAGCT GGTTCTTTCC GCCTCAGGAC TCTTCCTTTT TCAATATTAT 
     6121 TGAAGCATTT ATCAGGGTTA TTGTCTCATG AGCGGATACA TATTTGAATG TATTTAGAAA 
     6181 AATAAACAAA TAGGGGTTCC GCGCACATTT CCCCGAAAAG TGCCACCTGA CGCGCCCTGT 
     6241 AGCGGCGCAT TAAGCGCGGC GGGTGTGGTG GTTACGCGCA GCGTGACCGC TACACTTGCC 
     6301 AGCGCCCTAG CGCCCGCTCC TTTCGCTTTC TTCCCTTCCT TTCTCGCCAC GTTCGCCGGC 
     6361 TTTCCCCGTC AAGCTCTAAA TCGGGGGCTC CCTTTAGGGT TCCGATTTAG TGCTTTACGG 
     6421 CACCTCGACC CCAAAAAACT TGATTAGGGT GATGGTTCAC GTAGTGGGCC ATCGCCCTGA 
     6481 TAGACGGTTT TTCGCCCTTT GACGTTGGAG TCCACGTTCT TTAATAGTGG ACTCTTGTTC 
     6541 CAAACTGGAA CAACACTCAA CCCTATCTCG GTCTATTCTT TTGATTTATA AGGGATTTTG 
     6601 CCGATTTCGG CCTATTGGTT AAAAAATGAG CTGATTTAAC AAAAATTTAA CGCGAATTTT 
//