LOCUS       RR212929                7968 bp    DNA     circular  SYN 
DEFINITION  Tlr8 (Myc-DDK-tagged ORF) - Rat toll-like receptor 8 (Tlr8), (10 ug)
ACCESSION   NM_001101009
VERSION     NM_001101009.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4125..4216 
                     /label="Myc-DDK"  
     misc_feature    4246..4831 
                     /label="PolyA signal"  
     misc_feature    4303..4322 
                     /label="Restriction Enzyme"  
     misc_feature    5107..5779 
                     /label="ColE1"  
     misc_feature    6209..7003 
                     /label="Kan/Neo"  
     misc_feature    7038..7340 
                     /label="SV40 ori"  
     misc_feature    7426..7903 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4107..4113 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4107 
                     /label="Tlr8"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCCCCTCAG TCGTGGATTC TGACCTGCTT TTGCCTGCTC TCCTCTGGAA 
     1081 CCAGTGCAGT CTTCCTCAAA GGGAACTTTT CCAGAAGCTA TCCCTGTGAT GAGAAAAGGC 
     1141 ACAATGCCCT TGTGACTGCA GAATGCAATC ATCGTCAACT GCATGAAGTT CCCCAAACTA 
     1201 TCGGCAAATA TGTGACAGAT GTAGACTTGT CAGACAATAC TATTATGCAC ATAACAAATG 
     1261 AGTCCTTTCA GAAGTTTCGA AACCTCACTA AAATCAATCT GAACCACAAT GCCAAACAAC 
     1321 AGCACCCAAA TGAAAATAAA AATGGTATGA ATATTACAGA AGGGGCTTTC CTCAGCCTAA 
     1381 GAAATCTAAC TGAATTACTG TTGGAAGACA ACCAGTTATA TACTATACCT GCTGGGTTGC 
     1441 CTGAGTCTTT GAAAGAACTT AGCCTAATTC AAAACAATAT ATTTCAGGTA ACTAAAAACA 
     1501 ACACTTTCGG GCTCAGGAAC TTGGAAAGAC TCTATTTGGG CTGGAACTGC TATTTTAAGT 
     1561 GTAATCAAAT CTTTAAAGTA GAAGATGGGG CATTTAACAA TCTTATAAAC TTGAAGCTTC 
     1621 TCTCATTATC TTTCAATAAC CTTTTCTCCG TGCCTCCCAA ACTACCAAGC TCTCTAAGCA 
     1681 AACTTTTTCT GAGCAATGCC AAGATCTCAA CCATCACTCA GGAAGACTTC AAAGGACTGG 
     1741 AACATTTAAT ATTACTAGAT TTGAGTGGAA ACTGCCCAAG GTGTTTCAAT GCCCCATTTC 
     1801 CTTGTGAATC TTGCAACTTA AGTGCGTCCA TCAGAATACA TCCTCTTGCT TTTCAAAATC 
     1861 TCACTCAACT TCGCTTTCTT AACCTCTCTA GCACTTCCCT CAGGACGATT CCTTCTACCT 
     1921 GGTTCGACAA TCTGACAAAT TTGAAGGAAC TCCATCTTGA ATTCAATTAT TTAGTTCAAG 
     1981 AAATTGCCTC AGGAGCATTT TTAACCAAGC TACCCAGTTT ACAAACCCTT GACCTGTCCT 
     2041 TCAACTTTAT ACATAAAGAA TATTTACAAT ATATTACTAT CTCCCCGAAC TTCTCTATGC 
     2101 TTCGTTCTCT CAGGAAGTTG CACTTAAAAG GTTATGTGTT CCGAGAACTT AAAAAGGAAC 
     2161 ATTTCAAGCC CCTCCAGAAT CTTCCAAACT TAACAACCAT TAACTTGGGC ATTAACTTTA 
     2221 TTGAGAAAAT TGATTTCAAA GCTTTCCAGG ATTTTCCCAA CCTCAAAGTT ATTTACTTAT 
     2281 CGGGAAATCG CATAGCATCC GTAATAGATG GTACAGACCA CTCCTCTTGG CGAAACCGTC 
     2341 TTAGGAAGCC TCTCTCAACA GACTATGACG AGTTTGATCC ACACATGAAT TTTTACCATA 
     2401 GCACTGAACC TTTAATAAAG CCACAGTGTA CTACTTATGG CAAGGCCTTG GATTTAAGTT 
     2461 TGAACAATAT TTTCGTTATT GGGAAAAGTC AATTTGAAGG TTTTCAGGAT ATCGCCTGCT 
     2521 TAAATCTGTC TTTCAATGCC AATGGTCAAG TGTTGAATGG CACAGAATTC TCATCCATGC 
     2581 CCCACATTAA ATACTTGGAT TTAACCAACA ACAGACTAGA CTTTGATGAT AACCAGACCT 
     2641 TCAGTGACCT TCATGATCTA GAAGTGTTGG ACCTGAGCCA CAATGCACAC TATTTCAGTA 
     2701 TAGCAGGGGT AACACACCGT CTAGGATTTA TCCAGAACTT AATAAAGCTC AAGGTGTTAA 
     2761 ACCTGAGCCA CAATGGCATT TACACGCTCA CAGATGAATA CAAGCTGCAA AGCAAATCTC 
     2821 TGAAAGAGTT GGTTTTTAGT GGAAATCGCC TTGACCGTCT GTGGAATGCA AATGATGGCA 
     2881 AATACTGGTC CATTTTTACA AGTCTCGAGA CTCTGACACG CTTGGACTTA TCGTACAATA 
     2941 ACCTTCAACA AATCCCAAAT GAAGCATTCC TCAACTTGCC TCAGAGCCTC CAAGAGCTAC 
     3001 ATATCAATGA TAACAGATTA CGTTTCTTTA ATTGGACATT ACTCCAGTAT TTTCCTCACC 
     3061 TTCATGTGCT GGACTTGGGG AGAAATGAGC TGTATTTTCT AACCAATTGC CTATCGAAGT 
     3121 TTACACATTC CCTGAAGACA CTGCTGCTGA ACCACAATCA TTTCTCTCAC CTACCTGCTG 
     3181 GCTTCCTCTC CGAAGCCAGG AATCTGGTGT ACCTGGATCT AAGTTTCAAC ACAATAAAGA 
     3241 TGATCAATAA ATCCTCCCTG CAAACTGAGA CAAAAACAAA CTTGTCTGTT CTGGATCTAC 
     3301 AAGGGAACCA TTTTGACTGC ACGTGTGACA TAAGTGATTT CCGAAGCTGG CTAGAAGAAA 
     3361 ATCCGCATGT CAGAATTCCT AGATTGGTAG ATGTCATTTG TTCCAATCCT GGGGATCAGA 
     3421 GATGGAAGAG TGTCATGAGC CTAGATCTCA CGACTTGTGT GTCAGATACC ACTGCAGCTA 
     3481 TCCTGTTCTT CTTCACATTC CTTACCACCT CCACAGTGCT GTTGGCTGCT CTGGTTCATC 
     3541 ATCTGTTTTA CTGGGATGTT TGGTTTATCT ATCACATGTG CTCTGCTAAG TTAAGAGGCT 
     3601 ACAGGTCTTC ATCCACATCC CAAACTTTCT ATGATGCTTA CATTTCTTAT GACACCAAAG 
     3661 ACGCATCCGT CACTGACTGG GTGATCAATG AGCTGCGCTA CCACCTTGAA GAGAGTGAGG 
     3721 ACAAAAGTGT CCTCCTTTGC TTAGAGGAGA GGGATTGGGA TCCGGGATTA CCTATCATTG 
     3781 ATAACCTCAT GCAGAGCATA AACCAGAGCA AGAAGACAAT CTTTGTTTTA ACCAAGAAAT 
     3841 ATGCCAAGAG CTGGAACTTT AAAACCGCTT TCTACTTGGC CTTGCAGAGG CTAATGGATG 
     3901 AGAACATGGA TGTGATTATC TTCATCCTCC TGGAACCAGT GTTACAGTAT TCACAGTACC 
     3961 TGAGGCTTCG GCAGAGGATC TGTAAGAGCT CCATCCTCCA GTGGCCCAAC AACCCCAAAG 
     4021 CGGAAAACTT GTTTTGGCAA AGTCTTAAAA ATGTGGTCTT GACCGAAAAT GATTCACGGT 
     4081 ATGACAATTT GTACATCGAT TCCATTAGGC AATACACGCG TACGCGGCCG CTCGAGCAGA 
     4141 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     4201 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     4261 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4321 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4381 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4441 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4501 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4561 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4621 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4681 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4741 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4801 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4861 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4921 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4981 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     5041 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     5101 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     5161 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     5221 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     5281 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5341 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5401 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5461 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5521 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5581 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5641 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5701 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5761 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5821 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5881 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5941 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     6001 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     6061 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     6121 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     6181 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     6241 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6301 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6361 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6421 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6481 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6541 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6601 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6661 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6721 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6781 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6841 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6901 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6961 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     7021 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     7081 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     7141 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     7201 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     7261 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7321 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7381 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7441 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7501 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7561 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7621 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7681 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7741 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7801 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7861 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7921 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//