LOCUS       RR212846                8205 bp    DNA     circular  SYN 
DEFINITION  Eif2ak3 (Myc-DDK-tagged ORF) - Rat eukaryotic translation initiation factor 2 alpha kinase 3 (Eif2ak3), (10 ug)
ACCESSION   NM_031599
VERSION     NM_031599.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4362..4453 
                     /label="Myc-DDK"  
     misc_feature    4483..5068 
                     /label="PolyA signal"  
     misc_feature    4540..4559 
                     /label="Restriction Enzyme"  
     misc_feature    5344..6016 
                     /label="ColE1"  
     misc_feature    6446..7240 
                     /label="Kan/Neo"  
     misc_feature    7275..7577 
                     /label="SV40 ori"  
     misc_feature    7663..8140 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4344..4350 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4344 
                     /label="Eif2ak3"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCGCGCC ACCCAGCCCC GACCGCGCGC GCTGCTGCTG CTGTTCCTGC 
     1081 TGCTGGGCTG CGCGGCGGGG ATCTCAGCGG TCGCGCGCGC CCGCAGCTTG CTTGCTCCCA 
     1141 CATCGGATAC GGCATTTGGC TTGGGGGCAG CAGCCGCCCC TACTTCAGCC GCGCGGGTGC 
     1201 CTGCGGTGGC TACGGCCGAA GTGACCGTGG AAGACGCCGA GGCATTGCCC GCTGCCTCCG 
     1261 GCGAACAGGA GTCACGCGCG ACCGAGTCCG ATGACGACGT GGAACTGCGG CCTCGCGGCA 
     1321 GGTCCTTAGT AATCATCAGC ACTTTAGATG GACGAATTGC CGCACTGGAT GCCGAGAATC 
     1381 ATGGGAAAAA GCAGTGGGAT TTGGACGTGG GATCTGGTTC CTTGGTTTCA TCTAGCCTTA 
     1441 GCAAGCCAGA GGTGTTTGGG AACAAGATGA TCATTCCTTC CCTGGACGGA GACCTCTTCC 
     1501 AGTGGGACCG AGACCGAGAG AGCATGGAGG CCGTCCCTTT CACTGTGGAG TCCCTTCTCG 
     1561 AATCTTCATA CAAGTTTGGA GATGATGTTG TTCTGGTTGG AGGAAAGTCT CTGACCACAT 
     1621 ACGGGCTCAG TGCATATAGT GGAAAGCTGA GGTATATCTG TTCTGCCTTG GGATGTCGCC 
     1681 GATGGGATAG TGATGAAATG GAAGAAGAGG AAGACATCTT GCTTCTGCAA CGCACGCAGA 
     1741 AGACTGTGCG AGCTGTCGGG CCTCGAAGCG GCAGTGAGAA GTGGAATTTC AGTGTTGGCC 
     1801 ACTTTGAACT TCGGTATATT CCAGACATGG AAACTAGAGC CGGATTCATT GAAAGCACCT 
     1861 TTAAACTGGG TGGAAACAAA GAAGACTCTA AAATCATTTC AGATGTGGAA GAACAAGATG 
     1921 TGGACACAGT GATAAAAGTT TCCGTTGCTG ATTGGAAGGT CATGGCGTTT AGTAAGAAGG 
     1981 GAGGCCGCCT AGAATGGGAG TACCAGTTTT GTACTCCCAT TGCGTCTGCC TGGCTGGTGA 
     2041 GGGATGGTAA AGTCATCCCC ATCAGTCTGT TTGATGACAC AAGTTACACA GCCAATGAGG 
     2101 AAGTTTTGGA AGATGAAGAA GACATTGTAG AGGCTGCTCG GGGAGCCACA GAGAACAGCG 
     2161 TGTACTTAGG GATGTACAGA GGCCAGCTGT ACCTGCAATC ATCCGTCAGG GTCTCGGAAA 
     2221 AGTTCCCTAC AAGGCCGAAG GCCTTGGAGT CTGTAAATGG AGAAAGTGCC ATTATTCCTC 
     2281 TGCCGACGAT CAAATGGAAG CCCTTAATCC ATTCTCCGTC TAGGACTCCT GTCTTGGTTG 
     2341 GGTCTGATGA ATTTGACAAG TGTCTAAGTA ATGATAAATA TTCCCATGAA GAATACAGTA 
     2401 ATGGTGCGCT TTCAATCCTA CAGTATCCAT ATGACAACGG TTACTATCTG CCTTATTACA 
     2461 AGAGAGAAAG GAATAAGCGG AGCACGCAGA TCACGGTCAG GTTCCTGGAC AGCCCGCACT 
     2521 ACAGCAAGAA CATCCGCAAG AAGGACCCCA TCCTCCTCCT GCACTGGTGG AAGGAAATAT 
     2581 TTGGAACGAT CTTGCTTTGC ATTGTGGCCA CGACCTTTAT CGTGCGCAGG CTTTTCCATC 
     2641 CTCAGCCCCA CAGGCAACGG AAGGAGTCTG AAACTCAGTG CCAAACTGAA AGTAAATACG 
     2701 ACTCTGTGAG TGCTGATAAC AGCGACAACA GCTGGAACGA CATTAAACAC TCAGGATACG 
     2761 TGTCCCGATA CCTAACAGAT TTTGAGCCAA TTCAGTGCAT GGGTCGAGGC GGCTTTGGCG 
     2821 TTGTCTTTGA AGCTAAGAAC AAAGTAGATG ACTGCAATTA CGCCATCAAG AGGATCCGTC 
     2881 TCCCAAACAG GGAGCTGGCA CGGGAGAAGG TAATGCGGGA AGTCAAAGCC TTGGCTAAGC 
     2941 TGGAGCACCC AGGCATCGTG AGGTATTTCA ATGCGTGGCT GGAAACACCA CCAGAGAAGT 
     3001 GGCAAGAGGA GATGGATGAA ATCTGGCTGA AGGACGAAAG CACAGACTGG CCACTCAGCT 
     3061 CCCCTAGCCC AATGGATGCC CCATCTGTTA AGATCCGGCA GATGGATCCT TTCTCTACAA 
     3121 AAGAACAGAT TGAAGTCATA GCTCCTTCTC CTGAAAGAAG TCGGTCTTTC TCAGTGGGCA 
     3181 TTTCCTGTGG CCGGACAAGC TCATCTGAGA GCCAGTTCTC TCCCCTGGAG TTCTCAGGGA 
     3241 CAGACTGTGG AGACAACAGT GACTCAGAGG ACGCAGCCCA CAACCTCCAG GACAGCTGCC 
     3301 TGACAGATTG CGACATGGAA GATGGTACAG TGGACGGCGA TGATGAGGGA CACTCCTTTG 
     3361 AACTTTGTCC TTCTGAAGCT TCTCCCTATA CCAGGTCAAG GGAGGGAACG TCCTCTTCCA 
     3421 TAGTGTTTGA AGACTCTGGC TGTGATAATG CGTCCAGTAA GGAGGACCCC AGAATGAATC 
     3481 GGCTGCATAA TGGCCACCAT TACGTTAATA AGCTAACTGA GTTCAAGCAC TCCAGCAGCA 
     3541 GGTCTTCTTC CGAAGCCACC TTGTCTACCT CTCCTACCAG GCCAACCACC CTAAGTTTAG 
     3601 ATTTCACCAG GAACACGGTG GACCGGCTCC AGCCCAGCTC CCCCAAGGTG TACCTGTACA 
     3661 TCCAGATGCA GCTGTGCAGG AAGGAGAACC TTAAAGACTG GATGAACCGG CGCTGCAGCA 
     3721 TGGAGGACAG GGAGCACAGA GTGTGCCTGC ACATCTTTCT GCAGATCGCA GAGGCAGTGC 
     3781 AGTTTCTGCA CAGCAAGGGA CTCATGCACA GGGACCTCAA GCCTTCCAAC ATATTCTTCA 
     3841 CAATGGATGA TGTGGTCAAA GTTGGGGACT TTGGGCTAGT GACTGCAATG GACCAGGATG 
     3901 AAGAAGAGCA GACAGTACTG ACTCCAATGC CAGCCTATGC TACACACACG GGACAAGTCG 
     3961 GGACCAAGCT GTACATGAGC CCAGAACAGA TTCATGGAAA CAACTACTCC CATAAAGTGG 
     4021 ACATCTTCTC TTTAGGCTTG ATTCTGTTTG AACTCCTTTA CCCATTCAGC ACTCAGATGG 
     4081 AACGAGTTAG GACTTTAACT GATGTAAGAA ATCTCAAGTT TCCACCACTG TTCACTCAGA 
     4141 AATATCCCCA AGAGCATATG ATGGTTCAAG ACATGCTTTC TCCATCCCCC ATGGAGCGGC 
     4201 CTGAAGCCAC AGACATCATT GAAAATGCTG TGTTTGAGAA CTTGGAGTTT CCTGGGAAAA 
     4261 CGGTTCTGAG ACAGCGGTCC CGCTCCTTGA GTTCATCCGG AACTAAACAT TCCAGACAGC 
     4321 CCAGCAGCAC ATTCAGCCCA CTGCCTGGCA ACACGCGTAC GCGGCCGCTC GAGCAGAAAC 
     4381 TCATCTCAGA AGAGGATCTG GCAGCAAATG ATATCCTGGA TTACAAGGAT GACGACGATA 
     4441 AGGTTTAAAC GGCCGGCCGC GGTCATAGCT GTTTCCTGAA CAGATCCCGG GTGGCATCCC 
     4501 TGTGACCCCT CCCCAGTGCC TCTCCTGGCC CTGGAAGTTG CCACTCCAGT GCCCACCAGC 
     4561 CTTGTCCTAA TAAAATTAAG TTGCATCATT TTGTCTGACT AGGTGTCCTT CTATAATATT 
     4621 ATGGGGTGGA GGGGGGTGGT ATGGAGCAAG GGGCAAGTTG GGAAGACAAC CTGTAGGGCC 
     4681 TGCGGGGTCT ATTGGGAACC AAGCTGGAGT GCAGTGGCAC AATCTTGGCT CACTGCAATC 
     4741 TCCGCCTCCT GGGTTCAAGC GATTCTCCTG CCTCAGCCTC CCGAGTTGTT GGGATTCCAG 
     4801 GCATGCATGA CCAGGCTCAG CTAATTTTTG TTTTTTTGGT AGAGACGGGG TTTCACCATA 
     4861 TTGGCCAGGC TGGTCTCCAA CTCCTAATCT CAGGTGATCT ACCCACCTTG GCCTCCCAAA 
     4921 TTGCTGGGAT TACAGGCGTG AACCACTGCT CCCTTCCCTG TCCTTCTGAT TTTAAAATAA 
     4981 CTATACCAGC AGGAGGACGT CCAGACACAG CATAGGCTAC CTGGCCATGC CCAACCGGTG 
     5041 GGACATTTGA GTTGCTTGCT TGGCACTGTC CTCTCATGCG TTGGGTCCAC TCAGTAGATG 
     5101 CCTGTTGAAT TGGGTACGCG GCCAGCGGCG AGCGGTATCA GCTCACTCAA AGGCGGTAAT 
     5161 ACGGTTATCC ACAGAATCAG GGGATAACGC AGGAAAGAAC ATGTGAGCAA AAGGCCAGCA 
     5221 AAAGGCCAGG AACCGTAAAA AGGCCGCGTT GCTGGCGTTT TTCCATAGGC TCCGCCCCCC 
     5281 TGACGAGCAT CACAAAAATC GACGCTCAAG TCAGAGGTGG CGAAACCCGA CAGGACTATA 
     5341 AAGATACCAG GCGTTTCCCC CTGGAAGCTC CCTCGTGCGC TCTCCTGTTC CGACCCTGCC 
     5401 GCTTACCGGA TACCTGTCCG CCTTTCTCCC TTCGGGAAGC GTGGCGCTTT CTCATAGCTC 
     5461 ACGCTGTAGG TATCTCAGTT CGGTGTAGGT CGTTCGCTCC AAGCTGGGCT GTGTGCACGA 
     5521 ACCCCCCGTT CAGCCCGACC GCTGCGCCTT ATCCGGTAAC TATCGTCTTG AGTCCAACCC 
     5581 GGTAAGACAC GACTTATCGC CACTGGCAGC AGCCACTGGT AACAGGATTA GCAGAGCGAG 
     5641 GTATGTAGGC GGTGCTACAG AGTTCTTGAA GTGGTGGCCT AACTACGGCT ACACTAGAAG 
     5701 AACAGTATTT GGTATCTGCG CTCTGCTGAA GCCAGTTACC TTCGGAAAAA GAGTTGGTAG 
     5761 CTCTTGATCC GGCAAACAAA CCACCGCTGG TAGCGGTGGT TTTTTTGTTT GCAAGCAGCA 
     5821 GATTACGCGC AGAAAAAAAG GATCTCAAGA AGATCCTTTG ATCTTTTCTA CGGGGTCTGA 
     5881 CGCTCAGTGG AACGAAAACT CACGTTAAGG GATTTTGGTC ATGAGATTAT CAAAAAGGAT 
     5941 CTTCACCTAG ATCCTTTTAA ATTAAAAATG AAGTTTTAAA TCAATCTAAA GTATATATGA 
     6001 GTAACCTGAG GCTATGGCAG GGCCTGCCGC CCCGACGTTG GCTGCGAGCC CTGGGCCTTC 
     6061 ACCCGAACTT GGGGGGTGGG GTGGGGAAAA GGAAGAAACG CGGGCGTATT GGCCCCAATG 
     6121 GGGTCTCGGT GGGGTATCGA CAGAGTGCCA GCCCTGGGAC CGAACCCCGC GTTTATGAAC 
     6181 AAACGACCCA ACACCGTGCG TTTTATTCTG TCTTTTTATT GCCGTCATAG CGCGGGTTCC 
     6241 TTCCGGTATT GTCTCCTTCC GTGTTTCAGT TAGCCTCCCC CTAGGGTGGG CGAAGAACTC 
     6301 CAGCATGAGA TCCCCGCGCT GGAGGATCAT CCAGCCGGCG TCCCGGAAAA CGATTCCGAA 
     6361 GCCCAACCTT TCATAGAAGG CGGCGGTGGA ATCGAAATCT CGTGATGGCA GGTTGGGCGT 
     6421 CGCTTGGTCG GTCATTTCGA ACCCCAGAGT CCCGCTCAGA AGAACTCGTC AAGAAGGCGA 
     6481 TAGAAGGCGA TGCGCTGCGA ATCGGGAGCG GCGATACCGT AAAGCACGAG GAAGCGGTCA 
     6541 GCCCATTCGC CGCCAAGCTC TTCAGCAATA TCACGGGTAG CCAACGCTAT GTCCTGATAG 
     6601 CGATCCGCCA CACCCAGCCG GCCACAGTCG ATGAATCCAG AAAAGCGGCC ATTTTCCACC 
     6661 ATGATATTCG GCAAGCAGGC ATCGCCATGG GTCACGACGA GATCCTCGCC GTCGGGCATG 
     6721 CTCGCCTTGA GCCTGGCGAA CAGTTCGGCT GGCGCGAGCC CCTGATGCTC TTCGTCCAGA 
     6781 TCATCCTGAT CGACAAGACC GGCTTCCATC CGAGTACGTG CTCGCTCGAT GCGATGTTTC 
     6841 GCTTGGTGGT CGAATGGGCA GGTAGCCGGA TCAAGCGTAT GCAGCCGCCG CATTGCATCA 
     6901 GCCATGATGG ATACTTTCTC GGCAGGAGCA AGGTGAGATG ACAGGAGATC CTGCCCCGGC 
     6961 ACTTCGCCCA ATAGCAGCCA GTCCCTTCCC GCTTCAGTGA CAACGTCGAG CACAGCTGCG 
     7021 CAAGGAACGC CCGTCGTGGC CAGCCACGAT AGCCGCGCTG CCTCGTCTTG CAGTTCATTC 
     7081 AGGGCACCGG ACAGGTCGGT CTTGACAAAA AGAACCGGGC GCCCCTGCGC TGACAGCCGG 
     7141 AACACGGCGG CATCAGAGCA GCCGATTGTC TGTTGTGCCC AGTCATAGCC GAATAGCCTC 
     7201 TCCACCCAAG CGGCCGGAGA ACCTGCGTGC AATCCATCTT GTTCAATCAT GCGAAACGAT 
     7261 CCTCATCCTG TCTCTTGATC GATCTTTGCA AAAGCCTAGG CCTCCAAAAA AGCCTCCTCA 
     7321 CTACTTCTGG AATAGCTCAG AGGCCGAGGC GGCCTCGGCC TCTGCATAAA TAAAAAAAAT 
     7381 TAGTCAGCCA TGGGGCGGAG AATGGGCGGA ACTGGGCGGA GTTAGGGGCG GGATGGGCGG 
     7441 AGTTAGGGGC GGGACTATGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7501 TGCTGGGGAG CCTGGGGACT TTCCACACCT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7561 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CCTAACTGAC ACACATTCCA 
     7621 CAGCTGGTTC TTTCCGCCTC AGGACTCTTC CTTTTTCAAT ATTATTGAAG CATTTATCAG 
     7681 GGTTATTGTC TCATGAGCGG ATACATATTT GAATGTATTT AGAAAAATAA ACAAATAGGG 
     7741 GTTCCGCGCA CATTTCCCCG AAAAGTGCCA CCTGACGCGC CCTGTAGCGG CGCATTAAGC 
     7801 GCGGCGGGTG TGGTGGTTAC GCGCAGCGTG ACCGCTACAC TTGCCAGCGC CCTAGCGCCC 
     7861 GCTCCTTTCG CTTTCTTCCC TTCCTTTCTC GCCACGTTCG CCGGCTTTCC CCGTCAAGCT 
     7921 CTAAATCGGG GGCTCCCTTT AGGGTTCCGA TTTAGTGCTT TACGGCACCT CGACCCCAAA 
     7981 AAACTTGATT AGGGTGATGG TTCACGTAGT GGGCCATCGC CCTGATAGAC GGTTTTTCGC 
     8041 CCTTTGACGT TGGAGTCCAC GTTCTTTAAT AGTGGACTCT TGTTCCAAAC TGGAACAACA 
     8101 CTCAACCCTA TCTCGGTCTA TTCTTTTGAT TTATAAGGGA TTTTGCCGAT TTCGGCCTAT 
     8161 TGGTTAAAAA ATGAGCTGAT TTAACAAAAA TTTAACGCGA ATTTT
//