LOCUS       RR212834                7794 bp    DNA     circular  SYN 
DEFINITION  Kdm4a (Myc-DDK-tagged ORF) - Rat jumonji domain containing 2A (Jmjd2a), (10 ug)
ACCESSION   NM_001107966
VERSION     NM_001107966.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3951..4042 
                     /label="Myc-DDK"  
     misc_feature    4072..4657 
                     /label="PolyA signal"  
     misc_feature    4129..4148 
                     /label="Restriction Enzyme"  
     misc_feature    4933..5605 
                     /label="ColE1"  
     misc_feature    6035..6829 
                     /label="Kan/Neo"  
     misc_feature    6864..7166 
                     /label="SV40 ori"  
     misc_feature    7252..7729 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3933..3939 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3933 
                     /label="Kdm4a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTGTTCGT GAGTTCCGCA AGATCGCCAA TAGTGACAAG TACTGTACTC 
     1081 CACGGTATAG TGAATTTGAA GAACTTGAGC GTAAATACTG GAAAAACCTC ACATTCAATC 
     1141 CTCCCATCTA TGGAGCAGAT GTGAATGGTA CTCTCTATGA ACAGCACGTT GATGAGTGGA 
     1201 ACATCGGCCG GCTGAAGACC ATCCTGGACC TGGTGGAGAA GGAGAGTGGG ATCACCATCG 
     1261 AGGGCGTGAA CACCCCCTAC CTGTACTTTG GCATGTGGAA GACGTCCTTT GCCTGGCACA 
     1321 CGGAAGACAT GGATCTGTAC AGCATCAACT ACCTGCACTT TGGAGAGCCA AAGTCTTGGT 
     1381 ACTCTGTTCC ACCTGAGCAT GGGAAACGCC TGGAGCGGCT CGCCAAAGGC TTTTTCCCAG 
     1441 GAAGTGCTCA AAGCTGTGAG GCTTTTCTCC GCCACAAGAT GACCCTGATC TCCCCATTAA 
     1501 TGCTGAAGAA GTATGGCATT CCCTTTGACA AGGTGACCCA AGAAGCCGGG GAGTTTATGA 
     1561 TCACTTTTCC ATATGGGTAC CATGCTGGTT TCAACCATGG CTTCAACTGT GCAGAGTCTA 
     1621 CCAATTTTGC TACCCGTCGG TGGATTGAAT ATGGCAAGCA GGCTGTACTG TGTTCCTGCA 
     1681 GAAAAGATAT GGTGAAGATC TCTATGGACG TGTTTGTCAG GAGGTTCCAG CCGGAACGCT 
     1741 ACAAACTTTG GAAAGCTGGG AAAGACAGCA CTGTTATTGA CCACACTCTG CCCACACCGG 
     1801 AAGCAGCTGA GTTTCTGAAG GACAGTGGAC TAACCCCAAG AGCTGGGAAT GAGGAGTGCC 
     1861 CAGAGGAGGA CTCGGAAGCG GCGGAGCAGG GAGAGGAGGG GGATGTGAAG AGAAGCCTGG 
     1921 CTAAGCACCG AATAGGGACA AAGAGACACC GAGTCTGTCT GGAAATACCA CAGGAGGTCA 
     1981 GTCAGAGCGA GCTCTTCCCC AAGGAAGAGC TGAGTTCTGG ACAGTATGAG ATGACAGAGT 
     2041 GCCAGGCCAC CCTCGCCCCA GTGAGGCCCA CCCACAGCTC CGTGCGGCAA GTTGAGGACA 
     2101 GCCTTCCCTT CCCAGATTAC TCCGACCCCA CTGAGGTCAA ATTCGAAGAG CTGAAGAACG 
     2161 TCAAACTAGA AGAGGAGGAT GAGGATGAGG AGCCGGAAGC AGCTGCACTG GACCTTTCTG 
     2221 TGAATCCCGA GTCTGTAGAA GGACGCCTCG TCTTCTCGGG CTCCAAAAAG AAAGCCTCCT 
     2281 CCAGCCTAGG CTCCAGTTCC TCTCAGGATT CAGTTTCTTC AGATTCCGAA ACCACCGAGT 
     2341 CTGTCTCTTG CCAGGGCCAA GAGAAAACAG TTCTCACGGT GCACAGCTAC TCCAGAGGAG 
     2401 ATGGCAAGGC TGCCCCAGGC GAGCCGAGTG TGAAGAGGCA GCGCAGCGCC CCCCGCAGCA 
     2461 TCAGCGAGCA GGAGCTGGCA GAGGTTGCAG ATGAGTACAT GCTCTCCCTG GAGGAGAATA 
     2521 AGAAGACCAA GGGTCGCCGC CAGCCCTTGA GCAAGCTCCC ACGCCATCAC CCGCTGGTGT 
     2581 TGCAGGAGTG CGGCAGCGAT GACGAGACTT CTGAACAGCT GACACCCGAG GAAGAGGCTG 
     2641 AGGAGACCGA GGCTTGGGCT AAGCCTCTGA GCCAGCTGTG GCAGAACCGA CCTCCAAACT 
     2701 TTGAGGCTGA AAAGGAATTC AATGAGCTCA TGGCCCAGCA GGCCCCTCAC TGTGCTGTGT 
     2761 GTATGATCTT CCAGACCTAC CATCAGGTGG AATTTGGAGG CTTCAGTCAG AGCTGTGGGA 
     2821 ATGCTTCAGA ACCAGCTGCC CAACCTCAGA GGACTAAGCC ATTGATCCCC GAAATGTGCT 
     2881 TTACCACAAC TGGCTGCAGC ACAGACATCA ATCTGTCCAC CCCTTACCTA GAGGAGGATG 
     2941 GCACCAGCAT GCTGGTGTCT TGTAAGAAGT GCAGTGTCCG GGTCCACGCC AGTTGCTATG 
     3001 GCGTCCCCCC TGCAAAGGCT TCTGAAGAAT GGATGTGCTC TCGATGTTCA GCCAATGCCC 
     3061 TGGAGGAAGA CTGCTGTTTA TGCTCACTGC GTGGAGGGGC CCTGCAGAGA GCAAACGACG 
     3121 ACAGATGGGT TCATGTTTCA TGTGCTGTGG CAATTCTAGA AGCAAGGTTT GTCAACATTG 
     3181 CTGAAAGAAG TCCAGTGGAT GTGAGCAAAA TCCCTCTGCC CCGCTTCAAA CTGAAGTGTG 
     3241 TCTTTTGTAA GAAGCGAAGA AAACGGAATG CTGGCTGTTG TGTGCAGTGC TCTCACGGCC 
     3301 GCTGCCCCAC TGCCTTCCAT GTGAGCTGTG CCCAGGCTGC CGGGGTGATG ATGCAACCCG 
     3361 ATGACTGGCC TTTTGTTGTC TTCATCACGT GCTTCCGCCA CAAGATTCCC AATTTGGAGC 
     3421 GAGCTAAGGG AGCCTTGCTG AGCATCACGG CCGGCCAGAA GGTCATCAGC AAGCACAAGA 
     3481 ACGGCCGCTT CTACCAGTGC GAGGTGGTCA GACTCACCAC CGAAACCTTC TACGAAGTCA 
     3541 ACTTTGATGA CGGCTCCTTC AGCGACAACC TTTATCCTGA GGACATAGTG AGTCAGGACT 
     3601 GTCTCCAACT GGGGCCTCCT GCTGAAGGCG AAGTGGTTCA AGTCAGATGG ACAGATGGTC 
     3661 AAGTCTATGG GGCTAAGTTT GTGGCCTCCC ACCCCATCCA GATGTACCAG GTGGAGTTCG 
     3721 AGGATGGCTC GCAGCTGGTG GTTAAGAGAG ATGATGTATA CACACTGGAT GAAGAGCTTC 
     3781 CCAAGAGAGT CAAGTCTAGA CTGTCAGTAG CTTCAGACAT GCGGTTCAAT GAGATTTTCA 
     3841 CAGAGAAGGA AGTAAAGCAA GAAAAGAAAA GGCAACGGGT CATCAACTCA AGGTACCGGG 
     3901 AGGATTACAT CGAGCCAGCA CTGTATCGGG CCATCATGGA GACGCGTACG CGGCCGCTCG 
     3961 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4021 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4081 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4141 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4201 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4261 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4321 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4381 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4441 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4501 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     4561 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     4621 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     4681 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     4741 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     4801 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     4861 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     4921 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     4981 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5041 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5101 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5161 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5221 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5281 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5341 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5401 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5461 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     5521 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     5581 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     5641 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     5701 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     5761 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     5821 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     5881 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     5941 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6001 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6061 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6121 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6181 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6241 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6301 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6361 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6421 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6481 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     6541 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     6601 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     6661 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     6721 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     6781 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     6841 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     6901 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     6961 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7021 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7081 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7141 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7201 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7261 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7321 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7381 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7441 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7501 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     7561 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     7621 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     7681 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     7741 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//