LOCUS       RR212831                7461 bp    DNA     circular  SYN 
DEFINITION  Lrrc8d (Myc-DDK-tagged ORF) - Rat leucine rich repeat containing 8 family, member D (Lrrc8d), (10 ug)
ACCESSION   NM_001008338
VERSION     NM_001008338.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3618..3709 
                     /label="Myc-DDK"  
     misc_feature    3739..4324 
                     /label="PolyA signal"  
     misc_feature    3796..3815 
                     /label="Restriction Enzyme"  
     misc_feature    4600..5272 
                     /label="ColE1"  
     misc_feature    5702..6496 
                     /label="Kan/Neo"  
     misc_feature    6531..6833 
                     /label="SV40 ori"  
     misc_feature    6919..7396 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3600..3606 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3600 
                     /label="Lrrc8d"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGAATGTTT ACCCTTGCGG AAGTTGCTTC ACTTAATGAC ATTCAGCCAA 
     1081 CTTACCGAAT CCTGAAGCCA TGGTGGGACG TGTTTATGGA TTACCTGGCG GTTGTTATGC 
     1141 TGATGGTAGC CATCTTTGCA GGGACCATGC AACTTACCAA AGATCAGGTG GTCTGTTTGC 
     1201 CAGTGTTGCC GTCGCCTGCA AATTCAAAGG CGCACACACC ACCCGGGAAT GCTGACGTCA 
     1261 CCACTGAAGT CCCCAAGATG GAGACAGCCA CACCCCAAGA CCAAAACGGG CAGACGACGA 
     1321 ATGACATTGC CTTTGGCACA TCTGCTGTGA CGCCTGACAT ACCTCTCCAA GCCACCTATT 
     1381 CACACGCAGA GTCCACCTTT CCCAGTCAGG AGACGAAGAA GGAGAAGAGA GACCCCACGG 
     1441 GCCGCAAAAC CAACTTGGAT TTTCAGCAGT ACGTATTTAT CAATCAGATG TGCTACCATC 
     1501 TGGCCCTTCC TTGGTATTCC AAGTACTTTC CATACCTTGC CCTTATACAC ACCATCATCC 
     1561 TTATGGTCAG TAGCAACTTT TGGTTCAAAT ATCCCAAAAC GTGCTCCAAG GTTGAGCATT 
     1621 TTGTTTCAAT ATTAGGAAAG TGTTTCGAAT CTCCCTGGAC TACTAAAGCG CTGTCCGAGA 
     1681 CAGCCTGTGA GGACTCTGAG GAGAACAAGC AGCGGATAAC GGGCGCCCAG ACCCTACCGA 
     1741 AGCACGTGTC CACCAGCAGC GATGAGGGGA GCCCCAGCGC CAGCACCCCC ATGATCAACA 
     1801 AAACGGGCTT CAAGTTCTCA GCTGAAAAGC CGGTGATCGA AGTTCCCAGC ATGACGATCC 
     1861 TGGACAAGAA GGACGGGGAG CAGGCCAAAG CCCTGTTTGA GAAGGTGAGG AAATTTCGTG 
     1921 CCCATGTGGA AGACAGTGAC TTGATCTACA AGCTCTACGT GGTCCAGACC CTCATCAAGA 
     1981 CCGCCAAGTT CATTTTTATC CTCTGCTACA CTGCAAACTT TGTCAACGCC ATCAGCTTCG 
     2041 AGCATGTCTG CAAGCCAAAA GTCGAGCACC TCACGGGGTA CGAGGTGTTC GAGTGCACTC 
     2101 ACAATATGGC CTACATGTTG AAGAAACTGC TCATCAGCTA CATCTCCATC ATCTGTGTCT 
     2161 ATGGCTTCAT CTGCCTCTAC ACTCTCTTCT GGCTGTTCAG GATCCCTCTG AAGGAATATT 
     2221 CCTTTGAGAA AGTCCGAGAG GAAAGCAGCT TCAGCGACAT CCCGGATGTC AAGAACGACT 
     2281 TCGCGTTCCT CCTGCACATG GTCGACCAGT ACGACCAGCT CTATTCCAAG CGTTTTGGTG 
     2341 TCTTCTTATC TGAAGTCAGT GAAAACAAAC TGAGGGAAAT CAGCCTGAAC CATGAGTGGA 
     2401 CTTTCGAGAA ACTCAGGCAG CACGTGTCTC GCAACGCCCA GGACAAGCAG GAGCTGCACT 
     2461 TGTTCATGCT GTCAGGGGTG CCCGACGCTG TCTTTGACCT CACGGACCTG GATGTGCTAA 
     2521 AACTTGAACT GATTCCCGAA GCAAAAATTC CTGCCAAGAT CTCTCAGATG ACGAACCTTC 
     2581 AAGAACTCCA CCTCTGCCAC TGCCCTGCCA AGGTGGAGCA GACCGCCTTT AGCTTTCTTC 
     2641 GCGATCACTT GAGATGCCTT CATGTGAAGT TCACTGACGT GGCTGAAATC CCTGCCTGGG 
     2701 TGTATTTGCT CAAAAACCTG CGGGAGCTGT ACTTGATAGG CAACTTGAAC TCAGAGAACA 
     2761 ATAAGATGAT CGGGCTGGAG TCTCTGCGAG AGCTGCGGCA CCTTAAGATC CTCCACGTGA 
     2821 AGAGTAACCT GACCAAAGTT CCCTCCAACA TAACTGACGT GGCTCCTCAC CTCACCAAGC 
     2881 TCGTCATCCA CAATGACGGC ACCAAGCTCT TGGTGCTCAA CAGCCTGAAG AAGATGATGA 
     2941 ACGTTGCCGA GCTAGAGCTG CAGAACTGTG AGCTGGAGAG GATTCCACAC GCCATTTTCA 
     3001 GCCTTTCTAA CTTGCAGGAG CTGGACCTGA AGTCCAACAG CATCCGCACG ATCGAGGAGA 
     3061 TCATCAGTTT CCAACACCTG AAGCGACTGA CTTGCTTGAA ATTGTGGCAC AATAAAATTG 
     3121 TGGCCATTCC TCCCTCCATT ACCCACGTCA AGAACCTGGA GTCCCTCTAC TTCTCCAACA 
     3181 ACAAGCTCGA GTCCTTACCG GTGGCAGTGT TTAGTTTACA GAAACTCAGA TGCTTAGATG 
     3241 TCAGCTATAA CAACATTTCC ACGATCCCCA TAGAGATAGG TTTGCTTCAG AACCTGCAGC 
     3301 ATTTGCACAT CACAGGGAAC AAAGTGGACG TTCTGCCAAA ACAGTTGTTT AAGTGCGTGA 
     3361 AGTTGAGGAC TTTGAACCTG GGGCAGAACT GCATCGCCTC CCTGCCTGAG AAGATCAGTC 
     3421 AGCTGTCCCA GCTCACTCAG CTGGAGCTGA AGGGCAACTG TCTCGACCGC CTGCCAGCCC 
     3481 AGCTGGGCCA GTGTCGGATG CTCAAGAAAA GCGGGCTTGT TGTAGAAGAC CAACTGTTTG 
     3541 ACACGCTGCC ACTGGAAGTC AAAGAGGCAT TGAATCAAGA CGTAAATGTC CCCTTTGCGA 
     3601 ACGGGATTAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     3661 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     3721 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     3781 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     3841 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     3901 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     3961 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     4021 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     4081 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     4141 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     4201 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     4261 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     4321 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     4381 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     4441 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     4501 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     4561 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     4621 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     4681 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     4741 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     4801 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     4861 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     4921 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     4981 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     5041 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     5101 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     5161 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     5221 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     5281 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     5341 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     5401 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     5461 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     5521 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     5581 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     5641 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     5701 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     5761 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     5821 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     5881 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     5941 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     6001 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     6061 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     6121 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     6181 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     6241 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     6301 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     6361 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     6421 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     6481 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     6541 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     6601 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     6661 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     6721 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     6781 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     6841 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     6901 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     6961 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     7021 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     7081 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     7141 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     7201 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     7261 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     7321 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     7381 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     7441 CAAAAATTTA ACGCGAATTT T
//