LOCUS       RR212726                6636 bp    DNA     circular  SYN 
DEFINITION  Tcf4 (Myc-DDK-tagged ORF) - Rat transcription factor 4 (Tcf4), (10 ug)
ACCESSION   NM_053369
VERSION     NM_053369.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2793..2884 
                     /label="Myc-DDK"  
     misc_feature    2914..3499 
                     /label="PolyA signal"  
     misc_feature    2971..2990 
                     /label="Restriction Enzyme"  
     misc_feature    3775..4447 
                     /label="ColE1"  
     misc_feature    4877..5671 
                     /label="Kan/Neo"  
     misc_feature    5706..6008 
                     /label="SV40 ori"  
     misc_feature    6094..6571 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2775..2781 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2775 
                     /label="Tcf4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACTAGCAGG GATCTTGGGT CACATGACAA TCTCTCTCCA CCTTTTGCCA 
     1081 ATTCCAGAAT ACAAAGTAAA ACAGAAAGGG GTTCATACTC ATCTTATGGG AGAGAAAACG 
     1141 TACAGGGTTG CCACCAGAGT CTCCTCGGAG GGGACATGGA TATGGGCAAC CCAGGAACCC 
     1201 TTTCGCCCAC CAAACCTGGC TCCCAGTACT ACCCATATTC CAGCAATAAC GCCCGACGGA 
     1261 GGCCTCTTCA CAGTAGTACC ATGGAGGTGC AGACAAAGAA AGTCAGAAAA GTTCCTCCAG 
     1321 GCTTGCCATC TTCAGTCTAC GCTCCTTCAG CCAGCACTGC CGACTACAAC AGGGACTCAC 
     1381 CAGGCTATTC TTCCTCCAAG CCAGCAGCCA GCACTTTCTC CAGCTCCTTC TTCATGCAAG 
     1441 ATGGCCATCA CAGCAGTGAC CCTTGGAGCT CCTCCAGTGG AATGAATCAG CCCGGCTATG 
     1501 GAGGGATGCT AGGCAACTCT CATATCCCAC AGTCTAGCAG CTACTGTAGC CTGCATCCAC 
     1561 ATGAACGTCT GAGCTATCCA TCGCACTCCT CGGCAGACAT CAACTCCAGT CTTCCTCCGA 
     1621 TGTCCACGTT CCATCGAAGT GGCACAAACC ATTACAGCAC CTCTTCCTGC ACACCTCCTG 
     1681 CCAACGGAAC AGACAGTATA ATGGCAAACA GAGGAACTGG GGCAGCAGGC AGCTCCCAGA 
     1741 CTGGAGACGC TCTGGGGAAA GCCCTGGCTT CGATCTATTC TCCTGATCAC ACGAACAACA 
     1801 GCTTCTCCTC CAACCCTTCA ACTCCTGTTG GCTCCCCTCC TTCACTCTCA GCAGGCACAG 
     1861 CTGTTTGGTC TCGAAACGGA GGACAGGCCT CGTCATCTCC CAATTACGAA GGACCCTTGC 
     1921 ACTCCTTGCA GAGCCGAATT GAAGACCGTT TAGAAAGACT GGACGACGCA ATTCATGTTC 
     1981 TCCGGAACCA CGCGGTGGGC CCATCCACAG CTGTGCCCGG TGGCCATGGG GACATGCATG 
     2041 GGATCATCGG ACCCTCTCAC AATGGAGCAA TGGGCAGTCT GGGCTCAGGA TATGGAACTG 
     2101 GCCTTCTCTC AGCCAACAGA CATTCACTCA TGGTCGGGGC CCACCGTGAA GATGGCGTGG 
     2161 CACTGAGAGG CAGCCATTCT CTCCTGCCAA ACCAGGTTCC GGTTCCACAG CTTCCGGTCC 
     2221 AGTCTGCAAC TTCCCCTGAC TTGAACCCAC CCCAAGACCC TTACAGAGGG ATGCCACCAG 
     2281 GCCTCCAGGG ACAGAGTGTG TCTTCCGGCA GCTCTGAGAT CAAGTCCGAC GACGAGGGCG 
     2341 ATGAGAACCT ACAGGACACA AAATCTTCCG AGGACAAGAA ACTAGACGAC GACAAGAAGG 
     2401 ATATCAAATC AATTACTAGC AATAACGATG ATGAGGACCT GACCCCGGAG CAGAAGGCCG 
     2461 AGCGTGAGAA GGAGCGGAGG ATGGCCAATA ACGCCCGTGA GCGCCTGAGG GTCCGTGATA 
     2521 TCAACGAGGC TTTCAAGGAG CTGGGCCGCA TGGTACAGCT CCACCTGAAG AGCGACAAGC 
     2581 CCCAGACCAA GCTCCTGATT CTCCACCAGG CAGTGGCTGT CATCCTCAGC CTGGAGCAGC 
     2641 AAGTGCGAGA AAGGAACCTG AACCCAAAAG CTGCCTGTCT GAAAAGAAGG GAGGAAGAGA 
     2701 AGGTGTCCTC AGAGCCTCCC CCGCTCTCCT TGGCTGGCCC ACACCCTGGG ATGGGAGACA 
     2761 CAGCGAATCA CATGGGTCAG ATGACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     2821 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     2881 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     2941 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3001 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3061 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3121 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     3181 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     3241 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     3301 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     3361 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     3421 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     3481 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     3541 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     3601 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     3661 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     3721 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     3781 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     3841 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     3901 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     3961 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4021 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4081 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4141 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     4201 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     4261 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     4321 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     4381 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     4441 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     4501 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     4561 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     4621 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     4681 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     4741 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     4801 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     4861 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     4921 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     4981 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5041 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5101 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     5161 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     5221 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     5281 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     5341 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     5401 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     5461 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     5521 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     5581 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     5641 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     5701 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     5761 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     5821 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     5881 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     5941 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6001 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6061 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6121 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     6181 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     6241 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     6301 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     6361 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     6421 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     6481 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     6541 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     6601 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//