LOCUS       RR212495                8523 bp    DNA     circular  SYN 
DEFINITION  Kdm3a (Myc-DDK-tagged ORF) - Rat jumonji domain containing 1A (Jmjd1a), (10 ug)
ACCESSION   NM_175764
VERSION     NM_175764.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4680..4771 
                     /label="Myc-DDK"  
     misc_feature    4801..5386 
                     /label="PolyA signal"  
     misc_feature    4858..4877 
                     /label="Restriction Enzyme"  
     misc_feature    5662..6334 
                     /label="ColE1"  
     misc_feature    6764..7558 
                     /label="Kan/Neo"  
     misc_feature    7593..7895 
                     /label="SV40 ori"  
     misc_feature    7981..8458 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4662..4668 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4662 
                     /label="Kdm3a"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTACAAATCT CTACTAGACA AAGCTGGCTT GGGATCCATA ACTTCTGTTC 
     1081 GCTTCCTTGG AGATCAGCAA AGTGTATTTG TTTCCAAAGA CCTTTTGAAA CCTATACAGG 
     1141 ATGTAAACAG TCTTCGGCTT TCCCTTACTG ATAATCAGAC GGTCAGTAAA GAGTTTCAAG 
     1201 CTTTGATTGT AAAACATTTG GATGAAAGCC ATCTTTTACA AGGTGACAAG AACCTTGTTG 
     1261 GTTCAGAAGT AAGAATTTAT AGCTTGGATC CATCTACTCA GTGGTTTTCA GCAACTGTTG 
     1321 TACATGGAAA CCCCTCATCC AAAACTCTTC AAGTCAACTG TGAGGAGATT CCAGCGCTGA 
     1381 AAATTGTTGA TCCAGCACTG ATTCACGTTG AAGTTGTACA CGATAATTTT GTAACGTGTG 
     1441 GTAATTCTAC AAGAATTGGA GCTGTGAAAC GCAAGTCTTC TGAGAATAAT GGAAGTTCAG 
     1501 TTTCTAAACA AGCAAAATCT TGCTCTGAGG TCTCTCCCAG TATGTGTCCT GTACAGTCTG 
     1561 TTCCCACAAC AGTGTGTAAG GAGATCCTGC TTGGCTGTAC TGCAGCAACC CCATCTAGCA 
     1621 ACAGACAGCA GAACACTCCC CAGGCAGCCA ATTCTCCACC TAACATTGGA GCAAAACTTC 
     1681 CTCAAGGATG TCACAAGCAG AGTTTACCAG AAGAAATTTC TTCCTGTCTA AATACAAAAT 
     1741 CTGAAGTACT GAGAACAAAA CCAGATGTTT GCAAAGCAGG ATTACTTTCT TCAAAATCTT 
     1801 CTCAGGTTGG AGCTGGAGAC TTGAAAATTC TGAGTGAGCC CAAAGGTAGC TGTATCCAGC 
     1861 CTAAAACAAA CACTGATCAG GAGAGCAGAC TGGAGTCTAC TCCACAACCA GTCACTGGCC 
     1921 TTACAAAGGA GTGCTTGGTT ACAAAGACTT CCTCTAAGGC AGAACTGGAC AATGCCACTG 
     1981 CTCCTGAACT GCAGAAGCGT CTAGAGCACA CAGCTTCCAC ACCCGATGGT CTTTCAGACA 
     2041 AGCCAGAAGT GGAAGCAGGT GTCACTAGGC TTAATAGCTG TTCAGAAAAG AAGGTTGGAC 
     2101 CTTCAGATTT AGGTTCGCAG TCACAGAATT TAAAGGAAAC TTCAGTAAAA GTCGATCATG 
     2161 ACAGCTGTTG TACAAGAAGC AGTAATAAAA CCCAGACTCC CCCAGCCCGG AAGTCAGTTT 
     2221 TGACAGACCC AGATAAACTC AAGAAGCTAC AGCAGAGTGG AGAGGCCTTT GTTCAGGATG 
     2281 ACTCCTGTGT TAACATCGTG GCACAGCTGC CCAAGTGTCG GGAGTGTCGA TTAGACAGCC 
     2341 TGCGCAAGGA TAAGGACCAG CAGAAAGACT CGCCAGTGTT CTGTCGCTTT TTTCACTTCA 
     2401 GGAGGTTACA ATTCAACAAA CATGGTGTTC TGCGGGTAGA AGGCTTCTTA ACACCAAACA 
     2461 AGTACGACAG TGAAGCGATT GGCTTGTGGC TGCCTTTGAC CAAAAATGTT GTGGGGACCG 
     2521 ATTTGGACAC AGCAAAGTAT ATCCTGGCCA ATATTGGAGA CCACTTCTGT CAAATGGTGA 
     2581 TTTCTGAGAA GGAGGCTATG TCAACTATTG AGCCACACAG GCAGGTTGCT TGGAAACGAG 
     2641 CTGTCAAAGG AGTTAGAGAA ATGTGTGATG TGTGTGACAC AACCATTTTC AACCTGCACT 
     2701 GGGTGTGCCC TCGGTGTGGG TTTGGAGTGT GTGTAGATTG CTACCGGCTG AAGAGGAAGA 
     2761 ATTGCCAACA GGGGGCTGCC TACAAGACTT TCTCTTGGAT AAGGTGTGTG AAGAGTCAAA 
     2821 TACATGAGCC TGAGAACCTG ATGCCCACGC AGATTATTCC TGGCAAAGCC CTCTACGATG 
     2881 TTGGAGACAT TGTGCATTCT GTCAGAGCAA AATGGGGTAT AAAGGCCAAT TGTCCCTGCT 
     2941 CCAACAGGCA GTTCAAGCTC TTCTCGAAGC CAGCCTTAAA GGAAGACCTG AAGCAGGCAT 
     3001 CCTTGTCTGG AGAAAAACCA AGTCTCGGCA CCATGGTACA GCAAAGCTCC CCTGTGTTGG 
     3061 AGCCAGCAGC CGTGTGTGGG GAAGCACCCT CCAAGCCAGC CAGCAATGTG AAGCCCATCT 
     3121 GTCCAGCCAA CACATCCCCT TTAAACTGGC TGGCTGACCT GACCAGTGGC AATGTCAACA 
     3181 AGGAAAACAA GGAAAAACAG CTGACTATGC CAATTTTAAA GAACGAAATC AAATGCCTTC 
     3241 CACCCTTGCC TCCTCTGAAC AAGTCCAGCA CTGTCCTCCA TACTTTTAAC AGCACAATTT 
     3301 TGACACCCGT GAGCAACAAT AATTCTGGTT TTCTTCGAAA TCTTTTGAAT TCATCCACAG 
     3361 GAAAGACAGA AAATGGACTA AAAAACACAC CCAAAATCCT TGATGATATC TTTGCCTCTT 
     3421 TGGTACAAAA CAAGACTTCT TCTGACTTAT CCAAGAGGCC TCAAGGACTG ACGATCAAGC 
     3481 CTAGCATTCT TGGTTTTGAC ACTCCTCACT ACTGGCTGTG TGACAACCGC CTGCTGTGCT 
     3541 TGCAAGACCC CAACAATAAG AGCAATTGGA ATGTCTTTAG GGAATGCTGG AAACAGGGGC 
     3601 AGCCAGTGAT GGTGTCGGGA GTGCATCATA AATTAAACAC TGAACTCTGG AAACCCGAGT 
     3661 CCTTCAGGAA AGAGTTTGGC GAGCAAGAAG TAGACCTAGT CAATTGTAGG ACCAATGAAA 
     3721 TCATCACAGG AGCCACAGTG GGAGACTTCT GGGATGGATT TGAAGATGTT CCAAACCGTT 
     3781 TGAAAAACGA AAAAGAAAAA GAACCAATGG TGTTGAAACT TAAGGACTGG CCGCCAGGAG 
     3841 AAGACTTTAG AGATATGATG CCTTCCAGGT TTGATGATCT GATGGCCAAC ATTCCACTGC 
     3901 CTGAGTATAC CAGGCGAGAT GGCAAACTGA ACCTGGCCTC TAGACTGCCA AACTACTTTG 
     3961 TACGGCCAGA CCTGGGCCCC AAGATGTACA ATGCTTATGG ATTGATCACT CCAGAAGATC 
     4021 GGAAATATGG GACCACGAAT CTTCACTTAG ATGTATCTGA TGCAGCCAAT GTCATGGTTT 
     4081 ATGTGGGAAT TCCCAAAGGA CAGTGTGAAC AAGAGGAAGA AGTCCTTAGA ACCATCCAAG 
     4141 ATGGAGATTC GGATGAACTC ACAATCAAGC GATTTATTGA AGGAAAAGAG AAGCCAGGCG 
     4201 CCCTTTGGCA CATATATGCT GCTAAAGACA CAGAAAAGAT AAGAGAATTC CTTAAAAAGG 
     4261 TATCTGAAGA GCAGGGTCAA GAAAACCCCG CAGACCATGA TCCTATTCAT GATCAGAGCT 
     4321 GGTATTTAGA CCGATCACTG AGAAAGCGCC TTTATCAAGA GTATGGAGTT CAAGGCTGGG 
     4381 CTATTGTACA ATTTCTTGGC GATGTGGTGT TTATCCCGGC AGGAGCTCCA CATCAGGTTC 
     4441 ATAACTTATA CAGCTGTATC AAAGTCGCTG AAGACTTCGT GTCTCCAGAG CATGTGAAAC 
     4501 ACTGCTTCTG GCTCACTCAG GAATTCCGTC ACTTGTCACA GACTCATACC AACCACGAAG 
     4561 ATAAACTGCA GGTAAAGAAT GTTATCTACC ATGCAGTAAA AGATGCAGTT GCTATGCTGA 
     4621 AAGCAGTGAA TCCAGTTTGG GCAAATGTTA ACTCCTCTGC ACATTGGAGA ACGCGTACGC 
     4681 GGCCGCTCGA GCAGAAACTC ATCTCAGAAG AGGATCTGGC AGCAAATGAT ATCCTGGATT 
     4741 ACAAGGATGA CGACGATAAG GTTTAAACGG CCGGCCGCGG TCATAGCTGT TTCCTGAACA 
     4801 GATCCCGGGT GGCATCCCTG TGACCCCTCC CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC 
     4861 ACTCCAGTGC CCACCAGCCT TGTCCTAATA AAATTAAGTT GCATCATTTT GTCTGACTAG 
     4921 GTGTCCTTCT ATAATATTAT GGGGTGGAGG GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG 
     4981 AAGACAACCT GTAGGGCCTG CGGGGTCTAT TGGGAACCAA GCTGGAGTGC AGTGGCACAA 
     5041 TCTTGGCTCA CTGCAATCTC CGCCTCCTGG GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC 
     5101 GAGTTGTTGG GATTCCAGGC ATGCATGACC AGGCTCAGCT AATTTTTGTT TTTTTGGTAG 
     5161 AGACGGGGTT TCACCATATT GGCCAGGCTG GTCTCCAACT CCTAATCTCA GGTGATCTAC 
     5221 CCACCTTGGC CTCCCAAATT GCTGGGATTA CAGGCGTGAA CCACTGCTCC CTTCCCTGTC 
     5281 CTTCTGATTT TAAAATAACT ATACCAGCAG GAGGACGTCC AGACACAGCA TAGGCTACCT 
     5341 GGCCATGCCC AACCGGTGGG ACATTTGAGT TGCTTGCTTG GCACTGTCCT CTCATGCGTT 
     5401 GGGTCCACTC AGTAGATGCC TGTTGAATTG GGTACGCGGC CAGCGGCGAG CGGTATCAGC 
     5461 TCACTCAAAG GCGGTAATAC GGTTATCCAC AGAATCAGGG GATAACGCAG GAAAGAACAT 
     5521 GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT 
     5581 CCATAGGCTC CGCCCCCCTG ACGAGCATCA CAAAAATCGA CGCTCAAGTC AGAGGTGGCG 
     5641 AAACCCGACA GGACTATAAA GATACCAGGC GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC 
     5701 TCCTGTTCCG ACCCTGCCGC TTACCGGATA CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT 
     5761 GGCGCTTTCT CATAGCTCAC GCTGTAGGTA TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA 
     5821 GCTGGGCTGT GTGCACGAAC CCCCCGTTCA GCCCGACCGC TGCGCCTTAT CCGGTAACTA 
     5881 TCGTCTTGAG TCCAACCCGG TAAGACACGA CTTATCGCCA CTGGCAGCAG CCACTGGTAA 
     5941 CAGGATTAGC AGAGCGAGGT ATGTAGGCGG TGCTACAGAG TTCTTGAAGT GGTGGCCTAA 
     6001 CTACGGCTAC ACTAGAAGAA CAGTATTTGG TATCTGCGCT CTGCTGAAGC CAGTTACCTT 
     6061 CGGAAAAAGA GTTGGTAGCT CTTGATCCGG CAAACAAACC ACCGCTGGTA GCGGTGGTTT 
     6121 TTTTGTTTGC AAGCAGCAGA TTACGCGCAG AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT 
     6181 CTTTTCTACG GGGTCTGACG CTCAGTGGAA CGAAAACTCA CGTTAAGGGA TTTTGGTCAT 
     6241 GAGATTATCA AAAAGGATCT TCACCTAGAT CCTTTTAAAT TAAAAATGAA GTTTTAAATC 
     6301 AATCTAAAGT ATATATGAGT AACCTGAGGC TATGGCAGGG CCTGCCGCCC CGACGTTGGC 
     6361 TGCGAGCCCT GGGCCTTCAC CCGAACTTGG GGGGTGGGGT GGGGAAAAGG AAGAAACGCG 
     6421 GGCGTATTGG CCCCAATGGG GTCTCGGTGG GGTATCGACA GAGTGCCAGC CCTGGGACCG 
     6481 AACCCCGCGT TTATGAACAA ACGACCCAAC ACCGTGCGTT TTATTCTGTC TTTTTATTGC 
     6541 CGTCATAGCG CGGGTTCCTT CCGGTATTGT CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT 
     6601 AGGGTGGGCG AAGAACTCCA GCATGAGATC CCCGCGCTGG AGGATCATCC AGCCGGCGTC 
     6661 CCGGAAAACG ATTCCGAAGC CCAACCTTTC ATAGAAGGCG GCGGTGGAAT CGAAATCTCG 
     6721 TGATGGCAGG TTGGGCGTCG CTTGGTCGGT CATTTCGAAC CCCAGAGTCC CGCTCAGAAG 
     6781 AACTCGTCAA GAAGGCGATA GAAGGCGATG CGCTGCGAAT CGGGAGCGGC GATACCGTAA 
     6841 AGCACGAGGA AGCGGTCAGC CCATTCGCCG CCAAGCTCTT CAGCAATATC ACGGGTAGCC 
     6901 AACGCTATGT CCTGATAGCG ATCCGCCACA CCCAGCCGGC CACAGTCGAT GAATCCAGAA 
     6961 AAGCGGCCAT TTTCCACCAT GATATTCGGC AAGCAGGCAT CGCCATGGGT CACGACGAGA 
     7021 TCCTCGCCGT CGGGCATGCT CGCCTTGAGC CTGGCGAACA GTTCGGCTGG CGCGAGCCCC 
     7081 TGATGCTCTT CGTCCAGATC ATCCTGATCG ACAAGACCGG CTTCCATCCG AGTACGTGCT 
     7141 CGCTCGATGC GATGTTTCGC TTGGTGGTCG AATGGGCAGG TAGCCGGATC AAGCGTATGC 
     7201 AGCCGCCGCA TTGCATCAGC CATGATGGAT ACTTTCTCGG CAGGAGCAAG GTGAGATGAC 
     7261 AGGAGATCCT GCCCCGGCAC TTCGCCCAAT AGCAGCCAGT CCCTTCCCGC TTCAGTGACA 
     7321 ACGTCGAGCA CAGCTGCGCA AGGAACGCCC GTCGTGGCCA GCCACGATAG CCGCGCTGCC 
     7381 TCGTCTTGCA GTTCATTCAG GGCACCGGAC AGGTCGGTCT TGACAAAAAG AACCGGGCGC 
     7441 CCCTGCGCTG ACAGCCGGAA CACGGCGGCA TCAGAGCAGC CGATTGTCTG TTGTGCCCAG 
     7501 TCATAGCCGA ATAGCCTCTC CACCCAAGCG GCCGGAGAAC CTGCGTGCAA TCCATCTTGT 
     7561 TCAATCATGC GAAACGATCC TCATCCTGTC TCTTGATCGA TCTTTGCAAA AGCCTAGGCC 
     7621 TCCAAAAAAG CCTCCTCACT ACTTCTGGAA TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC 
     7681 TGCATAAATA AAAAAAATTA GTCAGCCATG GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT 
     7741 TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG GACTATGGTT GCTGACTAAT TGAGATGCAT 
     7801 GCTTTGCATA CTTCTGCCTG CTGGGGAGCC TGGGGACTTT CCACACCTGG TTGCTGACTA 
     7861 ATTGAGATGC ATGCTTTGCA TACTTCTGCC TGCTGGGGAG CCTGGGGACT TTCCACACCC 
     7921 TAACTGACAC ACATTCCACA GCTGGTTCTT TCCGCCTCAG GACTCTTCCT TTTTCAATAT 
     7981 TATTGAAGCA TTTATCAGGG TTATTGTCTC ATGAGCGGAT ACATATTTGA ATGTATTTAG 
     8041 AAAAATAAAC AAATAGGGGT TCCGCGCACA TTTCCCCGAA AAGTGCCACC TGACGCGCCC 
     8101 TGTAGCGGCG CATTAAGCGC GGCGGGTGTG GTGGTTACGC GCAGCGTGAC CGCTACACTT 
     8161 GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT TTCTTCCCTT CCTTTCTCGC CACGTTCGCC 
     8221 GGCTTTCCCC GTCAAGCTCT AAATCGGGGG CTCCCTTTAG GGTTCCGATT TAGTGCTTTA 
     8281 CGGCACCTCG ACCCCAAAAA ACTTGATTAG GGTGATGGTT CACGTAGTGG GCCATCGCCC 
     8341 TGATAGACGG TTTTTCGCCC TTTGACGTTG GAGTCCACGT TCTTTAATAG TGGACTCTTG 
     8401 TTCCAAACTG GAACAACACT CAACCCTATC TCGGTCTATT CTTTTGATTT ATAAGGGATT 
     8461 TTGCCGATTT CGGCCTATTG GTTAAAAAAT GAGCTGATTT AACAAAAATT TAACGCGAAT 
     8521 TTT
//