LOCUS       RR212478                7668 bp    DNA     circular  SYN 
DEFINITION  Dgkz (Myc-DDK-tagged ORF) - Rat diacylglycerol kinase zeta (Dgkz), (10 ug)
ACCESSION   NM_031143
VERSION     NM_031143.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3825..3916 
                     /label="Myc-DDK"  
     misc_feature    3946..4531 
                     /label="PolyA signal"  
     misc_feature    4003..4022 
                     /label="Restriction Enzyme"  
     misc_feature    4807..5479 
                     /label="ColE1"  
     misc_feature    5909..6703 
                     /label="Kan/Neo"  
     misc_feature    6738..7040 
                     /label="SV40 ori"  
     misc_feature    7126..7603 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3807..3813 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3807 
                     /label="Dgkz"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCCGCGG GACCCCAGCC CCGAGGCCCG GAGCAGCGAC TCGGAGTCGG 
     1081 CCTCCGCCTC GTCCAGCGGC TCCGAGCGCG ACGCGGATCC GGAGCCGGAC AAGGCTCCGC 
     1141 GGCGCTTAAC CAAGAGGCGC TTCCCGGGGC TGCGGCTCTT TGGGCACAGG AAAGCCATCA 
     1201 CCAAGTCGGG CCTCCAGCAC CTGGCACCCC CTCCTCCCAC GCCTGGGGCC CCGTGCGGTG 
     1261 AATCTGAGCG GCAGATCCGG AGTACTGTGG ACTGGAGTGA GTCAGCAGCG TATGGGGAGC 
     1321 ACATCTGGTT TGAGACCAAC GTGTCCGGAG ATTTCTGCTA TGTCGGGGAG CAGTACTGTG 
     1381 TAGCTAAGAT GCTGCCGAAG TCAGCGCCCA GAAGAAAGTG TGCAGCCTGT AAGATCGTGG 
     1441 TGCATACCCC TTGCATCGGG CAGCTGGAAA AGATCAATTT CCGCTGTAAG CCATCCTTCC 
     1501 GTGAATCAGG CTCCAGGAAT GTCCGTGAGC CAACCTTCGT AAGACACCAC TGGGTCCACA 
     1561 GGCGACGCCA GGATGGCAAG TGTCGGCACT GTGGGAAGGG CTTCCAGCAG AAGTTCACCT 
     1621 TCCACAGCAA AGAGATCGTA GCTATCAGCT GTTCCTGGTG CAAACAGGCG TACCACAGCA 
     1681 AGGTGTCCTG CTTCATGCTG CAGCAGATTG AGGAGCCCTG TTCCCTAGGG GTGCATGCAG 
     1741 CCGTGGTCAT CCCACCCACC TGGATCCTAC GGGCCCGGAG GCCCCAGAAC ACCCTCAAGG 
     1801 CCAGCAAGAA GAAAAAGAGA GCATCCTTCA AAAGGAGATC CAGTAAGAAA GGACCTGAGG 
     1861 AAGGCCGCTG GAGACCCTTC ATCATCAGGC CCACCCCGTC CCCCCTCATG AAGCCCCTGC 
     1921 TAGTGTTTGT AAACCCCAAG AGTGGGGGTA ACCAGGGCGC TAAGATCATC CAGTCTTTTC 
     1981 TGTGGTATCT GAACCCCCGG CAAGTCTTCG ATCTGAGCCA GGGAGGACCC AGGGAAGCGC 
     2041 TGGAAATGTA CCGCAAAGTC CATAATTTGA GGATTCTGGC TTGCGGGGGT GATGGCACGG 
     2101 TTGGCTGGAT TCTTTCCACC CTAGACCAGC TGCGCTTAAA ACCACCACCT CCTGTGGCCA 
     2161 TCCTGCCCCT GGGCACTGGC AATGACCTGG CCCGAACTCT CAACTGGGGT GGGGGTTACA 
     2221 CAGATGAGCC AGTGTCAAAG ATCCTTTCCC ATGTAGAGGA GGGGAACGTG GTACAGCTGG 
     2281 ACCGTTGGGA CCTCCGAGCA GAGCCCAACC CTGAGGCAGG GCCTGAGGAG CGAGATGACG 
     2341 GAGCCACTGA CCGGCTGCCC CTGGATGTCT TCAACAACTA CTTCAGTCTG GGCTTTGATG 
     2401 CCCACGTCAC CCTGGAGTTT CATGAGTCTC GAGAAGCCAA CCCAGAGAAG TTCAACAGCC 
     2461 GCTTTCGGAA TAAGATGTTC TATGCTGGGA CAGCCTTCTC TGACTTCCTG ATGGGCAGCT 
     2521 CCAAGGACTT AGCCAAGCAC ATCCGAGTAG TGTGTGACGG AATGGACCTA ACCCCCAAGA 
     2581 TTCAGGACCT GAAACCGCAG TGCATAGTCT TTCTGAATAT CCCCAGGTAC TGTGCAGGCA 
     2641 CCATGCCCTG GGGCCACCCT GGGGAGCACC ATGACTTCGA GCCCCAGAGG CACGATGATG 
     2701 GCTACCTCGA GGTCATCGGC TTCACCATGA CATCCTTGGC CGCATTGCAG GTGGGTGGAC 
     2761 ACGGCGAGCG GCTGACCCAG TGCCGAGAAG TGCTGCTCAC CACTGCCAAG GCCATCCCTG 
     2821 TGCAGGTGGA TGGTGAACCC TGCAAGCTTG CAGCATCGCG GATTCGAATT GCCCTGCGCA 
     2881 ACCAGGCCAC TATGGTGCAG AAGGCCAAGC GCCGGAGTAC TGCCCCACTG CACAGCGATC 
     2941 AGCAGCCAGT TCCTGAGCAG CTGAGGATCC AAGTGAGCAG GGTCAGCATG CACGACTACG 
     3001 AGGCTCTCCA TTATGACAAG GAGCAGCTCA AGGAGGCCTC TGTGCCTCTG GGTACTGTGG 
     3061 TGGTCCCCGG AGACAGTGAC CTAGAGCTCT GCCGAGCCCA CATTGAGAGA CTCCAGCAGG 
     3121 AGCCCGATGG GGCTGGAGCC AAGTCCCCCA TGTGCCACCC ACTGTCCTCC AAGTGGTGTT 
     3181 TCCTGGACGC CACCACTGCC AGCCGCTTCT ACAGGATCGA CAGGGCCCAG GAGCACCTCA 
     3241 ACTATGTGAC GGAGATTGCC CAGGATGAGA TTTATATCCT AGACCCAGAG CTGCTGGGTG 
     3301 CATCAGCCCG ACCTGACCTC CCTACCCCTA CCTCCCCGCT CCCTGCCTCC CCCTGCTCCC 
     3361 CTACACCCGG GTCACTGCAG GGGGATGCCG CACTGCCCCA AGGTGAAGAG CTGATTGAAG 
     3421 CTGCCAAGAG GAATGACTTC TGCAAGCTCC AGGAACTACA CCGAGCAGGA GGTGACCTCA 
     3481 TGCACCGCGA CCATCAGAGC CGCACACTCC TGCACCACGC AGTCAGCACT GGCAGTAAGG 
     3541 AAGTGGTCCG CTATCTGCTC GATCATGCAC CACCAGAGAT CCTCGATGCT GTGGAGGAGA 
     3601 ACGGGGAGAC CTGTCTACAC CAGGCAGCCG CCCTAGGTCA GCGCACCATC TGCCACTACA 
     3661 TTGTGGAAGC CGGGGCCTCC CTCATGAAGA CAGATCAGCA GGGCGACACT CCCAGGCAGC 
     3721 GAGCTGAGAA GGCTCAAGAC ACAGAGCTAG CTGCATACCT GGAGAACAGA CAACATTACC 
     3781 AGATGATCCA ACGTGAGGAC CAGGAGACAG CTGTGACGCG TACGCGGCCG CTCGAGCAGA 
     3841 AACTCATCTC AGAAGAGGAT CTGGCAGCAA ATGATATCCT GGATTACAAG GATGACGACG 
     3901 ATAAGGTTTA AACGGCCGGC CGCGGTCATA GCTGTTTCCT GAACAGATCC CGGGTGGCAT 
     3961 CCCTGTGACC CCTCCCCAGT GCCTCTCCTG GCCCTGGAAG TTGCCACTCC AGTGCCCACC 
     4021 AGCCTTGTCC TAATAAAATT AAGTTGCATC ATTTTGTCTG ACTAGGTGTC CTTCTATAAT 
     4081 ATTATGGGGT GGAGGGGGGT GGTATGGAGC AAGGGGCAAG TTGGGAAGAC AACCTGTAGG 
     4141 GCCTGCGGGG TCTATTGGGA ACCAAGCTGG AGTGCAGTGG CACAATCTTG GCTCACTGCA 
     4201 ATCTCCGCCT CCTGGGTTCA AGCGATTCTC CTGCCTCAGC CTCCCGAGTT GTTGGGATTC 
     4261 CAGGCATGCA TGACCAGGCT CAGCTAATTT TTGTTTTTTT GGTAGAGACG GGGTTTCACC 
     4321 ATATTGGCCA GGCTGGTCTC CAACTCCTAA TCTCAGGTGA TCTACCCACC TTGGCCTCCC 
     4381 AAATTGCTGG GATTACAGGC GTGAACCACT GCTCCCTTCC CTGTCCTTCT GATTTTAAAA 
     4441 TAACTATACC AGCAGGAGGA CGTCCAGACA CAGCATAGGC TACCTGGCCA TGCCCAACCG 
     4501 GTGGGACATT TGAGTTGCTT GCTTGGCACT GTCCTCTCAT GCGTTGGGTC CACTCAGTAG 
     4561 ATGCCTGTTG AATTGGGTAC GCGGCCAGCG GCGAGCGGTA TCAGCTCACT CAAAGGCGGT 
     4621 AATACGGTTA TCCACAGAAT CAGGGGATAA CGCAGGAAAG AACATGTGAG CAAAAGGCCA 
     4681 GCAAAAGGCC AGGAACCGTA AAAAGGCCGC GTTGCTGGCG TTTTTCCATA GGCTCCGCCC 
     4741 CCCTGACGAG CATCACAAAA ATCGACGCTC AAGTCAGAGG TGGCGAAACC CGACAGGACT 
     4801 ATAAAGATAC CAGGCGTTTC CCCCTGGAAG CTCCCTCGTG CGCTCTCCTG TTCCGACCCT 
     4861 GCCGCTTACC GGATACCTGT CCGCCTTTCT CCCTTCGGGA AGCGTGGCGC TTTCTCATAG 
     4921 CTCACGCTGT AGGTATCTCA GTTCGGTGTA GGTCGTTCGC TCCAAGCTGG GCTGTGTGCA 
     4981 CGAACCCCCC GTTCAGCCCG ACCGCTGCGC CTTATCCGGT AACTATCGTC TTGAGTCCAA 
     5041 CCCGGTAAGA CACGACTTAT CGCCACTGGC AGCAGCCACT GGTAACAGGA TTAGCAGAGC 
     5101 GAGGTATGTA GGCGGTGCTA CAGAGTTCTT GAAGTGGTGG CCTAACTACG GCTACACTAG 
     5161 AAGAACAGTA TTTGGTATCT GCGCTCTGCT GAAGCCAGTT ACCTTCGGAA AAAGAGTTGG 
     5221 TAGCTCTTGA TCCGGCAAAC AAACCACCGC TGGTAGCGGT GGTTTTTTTG TTTGCAAGCA 
     5281 GCAGATTACG CGCAGAAAAA AAGGATCTCA AGAAGATCCT TTGATCTTTT CTACGGGGTC 
     5341 TGACGCTCAG TGGAACGAAA ACTCACGTTA AGGGATTTTG GTCATGAGAT TATCAAAAAG 
     5401 GATCTTCACC TAGATCCTTT TAAATTAAAA ATGAAGTTTT AAATCAATCT AAAGTATATA 
     5461 TGAGTAACCT GAGGCTATGG CAGGGCCTGC CGCCCCGACG TTGGCTGCGA GCCCTGGGCC 
     5521 TTCACCCGAA CTTGGGGGGT GGGGTGGGGA AAAGGAAGAA ACGCGGGCGT ATTGGCCCCA 
     5581 ATGGGGTCTC GGTGGGGTAT CGACAGAGTG CCAGCCCTGG GACCGAACCC CGCGTTTATG 
     5641 AACAAACGAC CCAACACCGT GCGTTTTATT CTGTCTTTTT ATTGCCGTCA TAGCGCGGGT 
     5701 TCCTTCCGGT ATTGTCTCCT TCCGTGTTTC AGTTAGCCTC CCCCTAGGGT GGGCGAAGAA 
     5761 CTCCAGCATG AGATCCCCGC GCTGGAGGAT CATCCAGCCG GCGTCCCGGA AAACGATTCC 
     5821 GAAGCCCAAC CTTTCATAGA AGGCGGCGGT GGAATCGAAA TCTCGTGATG GCAGGTTGGG 
     5881 CGTCGCTTGG TCGGTCATTT CGAACCCCAG AGTCCCGCTC AGAAGAACTC GTCAAGAAGG 
     5941 CGATAGAAGG CGATGCGCTG CGAATCGGGA GCGGCGATAC CGTAAAGCAC GAGGAAGCGG 
     6001 TCAGCCCATT CGCCGCCAAG CTCTTCAGCA ATATCACGGG TAGCCAACGC TATGTCCTGA 
     6061 TAGCGATCCG CCACACCCAG CCGGCCACAG TCGATGAATC CAGAAAAGCG GCCATTTTCC 
     6121 ACCATGATAT TCGGCAAGCA GGCATCGCCA TGGGTCACGA CGAGATCCTC GCCGTCGGGC 
     6181 ATGCTCGCCT TGAGCCTGGC GAACAGTTCG GCTGGCGCGA GCCCCTGATG CTCTTCGTCC 
     6241 AGATCATCCT GATCGACAAG ACCGGCTTCC ATCCGAGTAC GTGCTCGCTC GATGCGATGT 
     6301 TTCGCTTGGT GGTCGAATGG GCAGGTAGCC GGATCAAGCG TATGCAGCCG CCGCATTGCA 
     6361 TCAGCCATGA TGGATACTTT CTCGGCAGGA GCAAGGTGAG ATGACAGGAG ATCCTGCCCC 
     6421 GGCACTTCGC CCAATAGCAG CCAGTCCCTT CCCGCTTCAG TGACAACGTC GAGCACAGCT 
     6481 GCGCAAGGAA CGCCCGTCGT GGCCAGCCAC GATAGCCGCG CTGCCTCGTC TTGCAGTTCA 
     6541 TTCAGGGCAC CGGACAGGTC GGTCTTGACA AAAAGAACCG GGCGCCCCTG CGCTGACAGC 
     6601 CGGAACACGG CGGCATCAGA GCAGCCGATT GTCTGTTGTG CCCAGTCATA GCCGAATAGC 
     6661 CTCTCCACCC AAGCGGCCGG AGAACCTGCG TGCAATCCAT CTTGTTCAAT CATGCGAAAC 
     6721 GATCCTCATC CTGTCTCTTG ATCGATCTTT GCAAAAGCCT AGGCCTCCAA AAAAGCCTCC 
     6781 TCACTACTTC TGGAATAGCT CAGAGGCCGA GGCGGCCTCG GCCTCTGCAT AAATAAAAAA 
     6841 AATTAGTCAG CCATGGGGCG GAGAATGGGC GGAACTGGGC GGAGTTAGGG GCGGGATGGG 
     6901 CGGAGTTAGG GGCGGGACTA TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6961 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCTGGTTGCT GACTAATTGA GATGCATGCT 
     7021 TTGCATACTT CTGCCTGCTG GGGAGCCTGG GGACTTTCCA CACCCTAACT GACACACATT 
     7081 CCACAGCTGG TTCTTTCCGC CTCAGGACTC TTCCTTTTTC AATATTATTG AAGCATTTAT 
     7141 CAGGGTTATT GTCTCATGAG CGGATACATA TTTGAATGTA TTTAGAAAAA TAAACAAATA 
     7201 GGGGTTCCGC GCACATTTCC CCGAAAAGTG CCACCTGACG CGCCCTGTAG CGGCGCATTA 
     7261 AGCGCGGCGG GTGTGGTGGT TACGCGCAGC GTGACCGCTA CACTTGCCAG CGCCCTAGCG 
     7321 CCCGCTCCTT TCGCTTTCTT CCCTTCCTTT CTCGCCACGT TCGCCGGCTT TCCCCGTCAA 
     7381 GCTCTAAATC GGGGGCTCCC TTTAGGGTTC CGATTTAGTG CTTTACGGCA CCTCGACCCC 
     7441 AAAAAACTTG ATTAGGGTGA TGGTTCACGT AGTGGGCCAT CGCCCTGATA GACGGTTTTT 
     7501 CGCCCTTTGA CGTTGGAGTC CACGTTCTTT AATAGTGGAC TCTTGTTCCA AACTGGAACA 
     7561 ACACTCAACC CTATCTCGGT CTATTCTTTT GATTTATAAG GGATTTTGCC GATTTCGGCC 
     7621 TATTGGTTAA AAAATGAGCT GATTTAACAA AAATTTAACG CGAATTTT
//