LOCUS       RR212469                8373 bp    DNA     circular  SYN 
DEFINITION  Man2a2 (Myc-DDK-tagged ORF) - Rat mannosidase 2, alpha 2 (Man2a2), (10 ug)
ACCESSION   NM_001107527
VERSION     NM_001107527.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4530..4621 
                     /label="Myc-DDK"  
     misc_feature    4651..5236 
                     /label="PolyA signal"  
     misc_feature    4708..4727 
                     /label="Restriction Enzyme"  
     misc_feature    5512..6184 
                     /label="ColE1"  
     misc_feature    6614..7408 
                     /label="Kan/Neo"  
     misc_feature    7443..7745 
                     /label="SV40 ori"  
     misc_feature    7831..8308 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4512..4518 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4512 
                     /label="Man2a2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCTGAAA AAGCAGGTGA CAGTGTGCGG GGCTGCTATC TTCTGTGTGG 
     1081 CCGTCTTTTC CCTGTACCTA ATGCTGGACC GAGTGCAGCA TGATCCTGCC AGACACCAGA 
     1141 ATGGTGGGAA CTTCCCCAGG AGCCAAATTT CTGTGCTACA GAACCGGATC GAACAGCTGG 
     1201 AACAGCTGCT GGAAGAAAAC CATGAGATCA TAAGCCATAT CAAGGACTCT GTGCTGGAAC 
     1261 TGACAGCCAA TGCGGAGGGC CCACCAGCCC TGCTGCCCTA CCACACAGCC AACGGCTCCT 
     1321 GGGCTGTGCT CCCCGAGCCC CGGCCCAGCT TCTTCTCTGT ATCCCCTGAG GACTGCCAGT 
     1381 TTGCTTTGGG GGGCCGGGGT CAGAAGCCAG AGCTACAGAT GTTAACTGTG TCTGAGGATT 
     1441 TGCCGTTTGA CAATGTGGAG GGCGGCGTGT GGAGGCAAGG CTTCGACATC TCCTACAGCC 
     1501 CAAATGACTG GGATGCTGAA GACCTGCAGG TGTTTGTGGT GCCTCACTCC CACAATGATC 
     1561 CAGGCTGGAT CAAGACTTTT GACAAGTACT ACACGGAACA AACCCAACAC ATCCTCAACA 
     1621 GCATGGTGTC CAAGCTGCAG GAAGATCCCC GACGGCGCTT TCTCTGGGCA GAAGTCTCCT 
     1681 TCTTCGCCAA GTGGTGGGAC AACATCAGTG CCCAGAAAAG GGCAGCAGTT CGAAGGCTGG 
     1741 TGGGAAATGG GCAGCTGGAA ATTGCAACGG GTGGATGGGT GATGCCAGAT GAGGCCAACT 
     1801 CCCATTACTT TGCCCTGGTC GACCAGCTCA TCGAGGGACA CCAGTGGCTG GAGAGAAACC 
     1861 TGGGTGCAAC TCCTCGCTCG GGCTGGGCAG TGGACCCCTT TGGACACAGC TCCACCATGC 
     1921 CTTACCTGCT GCGCCGTGCC AACCTGACCA GCATGCTAAT TCAGAGGGTG CATTACGCCA 
     1981 TCAAGAAGCA CTTTGCGGCC ACTCACAGCC TGGAGTTCAT GTGGAGGCAG ACATGGGATT 
     2041 CAGACTCCAG CACAGACATC TTCTGCCACA TGATGCCCTT CTACAGCTAC GACGTCCCAC 
     2101 ACACCTGTGG CCCTGATCCC AAGATCTGCT GCCAGTTTGA TTTCAAACGT CTGCCGGGTG 
     2161 GGAGAATCAA TTGTCCTTGG AAGGTGCCGC CGCGGGCTAT CACAGAGGCC AACGTGGCAG 
     2221 ACAGGGCAGC CCTGCTCCTG GACCAGTACC GGAAGAAGTC CCGGCTGTTT CGAAGCAGTG 
     2281 TCCTCCTTGT GCCGCTGGGT GATGACTTCC GATATGACAA GCCCCAGGAA TGGGATGCCC 
     2341 AGTTCTTCAA CTATCAACGG CTCTTTGACT TCCTCAACAG CAAGCCGGAG TTCCACGTAC 
     2401 AGGCACAGTT TGGGACCCTC TCTGAGTATT TTGATGCCCT GTATAAGAGG ACAGGAGTGG 
     2461 AGCCTGGTGC CCGGCCTCCA GGGTTTCCTG TGCTGAGTGG GGACTTCTTC TCCTATGCTG 
     2521 ACCGGGAGGA CCACTACTGG ACAGGCTATT ACACTTCCCG GCCTTTCTAT AAGAGCTTGG 
     2581 ACCGCGTGCT AGAAACTCAC CTTCGTGGGG CAGAGGTTCT ATACAGCCTG GCTTTGGCGC 
     2641 ATGCCCGCCG TTCTGGACTG ACTGGCCAGT ATCCGCTGTC TGATTACGCT GTCCTGACGG 
     2701 AAGCTCGTCG TACACTGGGC CTCTTCCAGC ACCACGATGC CATCACCGGA ACTGCCAAGG 
     2761 AGGCAGTTGT AGTAGACTAT GGGGTCAGGT TGCTGCGTTC CCTGGTCAGC CTAAAGCAGG 
     2821 TCATCATCAA TGCTGCCCAC TACCTGGTGC TGGGGGACAA GGAGACCTAC AGCTTTGACC 
     2881 CTAGGGCACC CTTCCTCCAA ATGGATGACA GTCGAGTAAG TCACGATGCC CTGCCTGAGC 
     2941 GCACGGTGAT CCGTCTGGAC TCCTCACCCA GGTTTGTGGT GGTCTTTAAC CCACTGGAAC 
     3001 AGGAGCGGCT CAGTGTGGTG TCCCTGCTGG TCAACTCACC CCGAGTGCGA GTGCTTTCAG 
     3061 AGGAGGGTCA GCCCTTGTCT GTGCAGATCA GTGTGCAGTG GAGCTCCGCC ACCAACATGG 
     3121 TCCCCGATGT CTACCAGGTG TCAGTGCCTG TCCGCCTGCC AGCCCTGGGC CTGGGTGTGC 
     3181 TGCAGCTGCA GCCAGATCTC GATGGACCCT ACACACTGCA GTCTTCGGTG CATGTCTACC 
     3241 TGAACGGCGT GAAACTGTCT GTCAGCAGGC AAACAACATT CCCTCTCCGT GTTGTGGACT 
     3301 CGGGCACCAG TGACTTCGCC ATCAGCAATC GATACATGCA GGTCTGGTTC TCCGGCCTTA 
     3361 CTGGGCTTCT CAAGAGCGTC CGACGTGTGG ACGAAGAGCA GGAACAGCAG GTGGACATGA 
     3421 AGCTCTTCGT CTATGGAACC CGCACATCCA AGGATAAGAG TGGTGCCTAC CTCTTCCTGC 
     3481 CTGATAACGA GGCTAAGCCC TATGTCCCTA AGAAACCTCC TGTGCTGCGT GTCACCGAAG 
     3541 GCCCTTTCTT CTCAGAGGTG GCTGCGTATT ATGAGCACTT TCACCAAGTG ATTCGACTTT 
     3601 ACAACCTGCC AGGGGTAGAG GGGCTGTCTC TGGACGTGTC GTTCCAGGTG GACATCAGGG 
     3661 ACTACGTGAA CAAGGAGCTA GCCCTGCGCA TCCACACAGA CATCGACAGC CAGGGCACTT 
     3721 TCTTCACAGA CCTCAATGGC TTTCAGGTAC AGCCCCGGAA GTATCTGAAG AAGTTGCCCC 
     3781 TGCAGGCTAA TTTCTACCCT ATGCCAGTCA TGGCCTACAT CCAGGATTCC CAGAGGCGCC 
     3841 TCACGCTGCA CACTGCTCAG GCTCTGGGTG TCTCCAGCCT CGGCAATGGC CAGCTGGAGG 
     3901 TGATCTTGGA CCGAAGGCTA ATGCAGGATG ACAACCGGGG ACTAGGCCAA GGGCTCAAAG 
     3961 ACAACAAGAT CACCTGCAAC CATTTCCGCC TCCTGTTAGA ACGTCGAACC CTGATGAGCC 
     4021 CTGAGGTCCA ACAGGAGCGC TCTACAAGCT ACCCGTCCCT CCTCAGCCAC ATGACTTCCA 
     4081 TGTACCTCAA CACACCTCCT CTGGTCTTAC CGGTGGCCAA GAGGGAGAGC ACCAGCCCCA 
     4141 CTCTGCACTC TTTCCACCCT CTGGCTTCTC CGTTGCCCTG CGATTTCCAT CTGCTCAATC 
     4201 TGCGCATGCT CCCCGCCGAG GTGAGTGTCC CGGTCCGTGC CAATCCTCAC CATCAGGCTG 
     4261 AGGACACCTT GCCCGCGGCT GATGCTGCTC TCATCCTACA CCGCAAGGGT TTTGACTGTG 
     4321 GCCTTGAAGC CAAGAACCTG GGCTTCAACT GTACCACAAG CCAAGGCAAG CTGGCCCTGG 
     4381 GGAGCCTCTT CCATGGCCTG GATGTGCTAT TCCTGCAGCC CACCTCTTTG ACTTTGCTAT 
     4441 ACCCTCTGGC CTCGCCCTCC AACAGCACTG ACATCTCTCT GGAGCCCATG GAGATCAGCA 
     4501 CCTTCCGCCT GCGCTTGGGT ACGCGTACGC GGCCGCTCGA GCAGAAACTC ATCTCAGAAG 
     4561 AGGATCTGGC AGCAAATGAT ATCCTGGATT ACAAGGATGA CGACGATAAG GTTTAAACGG 
     4621 CCGGCCGCGG TCATAGCTGT TTCCTGAACA GATCCCGGGT GGCATCCCTG TGACCCCTCC 
     4681 CCAGTGCCTC TCCTGGCCCT GGAAGTTGCC ACTCCAGTGC CCACCAGCCT TGTCCTAATA 
     4741 AAATTAAGTT GCATCATTTT GTCTGACTAG GTGTCCTTCT ATAATATTAT GGGGTGGAGG 
     4801 GGGGTGGTAT GGAGCAAGGG GCAAGTTGGG AAGACAACCT GTAGGGCCTG CGGGGTCTAT 
     4861 TGGGAACCAA GCTGGAGTGC AGTGGCACAA TCTTGGCTCA CTGCAATCTC CGCCTCCTGG 
     4921 GTTCAAGCGA TTCTCCTGCC TCAGCCTCCC GAGTTGTTGG GATTCCAGGC ATGCATGACC 
     4981 AGGCTCAGCT AATTTTTGTT TTTTTGGTAG AGACGGGGTT TCACCATATT GGCCAGGCTG 
     5041 GTCTCCAACT CCTAATCTCA GGTGATCTAC CCACCTTGGC CTCCCAAATT GCTGGGATTA 
     5101 CAGGCGTGAA CCACTGCTCC CTTCCCTGTC CTTCTGATTT TAAAATAACT ATACCAGCAG 
     5161 GAGGACGTCC AGACACAGCA TAGGCTACCT GGCCATGCCC AACCGGTGGG ACATTTGAGT 
     5221 TGCTTGCTTG GCACTGTCCT CTCATGCGTT GGGTCCACTC AGTAGATGCC TGTTGAATTG 
     5281 GGTACGCGGC CAGCGGCGAG CGGTATCAGC TCACTCAAAG GCGGTAATAC GGTTATCCAC 
     5341 AGAATCAGGG GATAACGCAG GAAAGAACAT GTGAGCAAAA GGCCAGCAAA AGGCCAGGAA 
     5401 CCGTAAAAAG GCCGCGTTGC TGGCGTTTTT CCATAGGCTC CGCCCCCCTG ACGAGCATCA 
     5461 CAAAAATCGA CGCTCAAGTC AGAGGTGGCG AAACCCGACA GGACTATAAA GATACCAGGC 
     5521 GTTTCCCCCT GGAAGCTCCC TCGTGCGCTC TCCTGTTCCG ACCCTGCCGC TTACCGGATA 
     5581 CCTGTCCGCC TTTCTCCCTT CGGGAAGCGT GGCGCTTTCT CATAGCTCAC GCTGTAGGTA 
     5641 TCTCAGTTCG GTGTAGGTCG TTCGCTCCAA GCTGGGCTGT GTGCACGAAC CCCCCGTTCA 
     5701 GCCCGACCGC TGCGCCTTAT CCGGTAACTA TCGTCTTGAG TCCAACCCGG TAAGACACGA 
     5761 CTTATCGCCA CTGGCAGCAG CCACTGGTAA CAGGATTAGC AGAGCGAGGT ATGTAGGCGG 
     5821 TGCTACAGAG TTCTTGAAGT GGTGGCCTAA CTACGGCTAC ACTAGAAGAA CAGTATTTGG 
     5881 TATCTGCGCT CTGCTGAAGC CAGTTACCTT CGGAAAAAGA GTTGGTAGCT CTTGATCCGG 
     5941 CAAACAAACC ACCGCTGGTA GCGGTGGTTT TTTTGTTTGC AAGCAGCAGA TTACGCGCAG 
     6001 AAAAAAAGGA TCTCAAGAAG ATCCTTTGAT CTTTTCTACG GGGTCTGACG CTCAGTGGAA 
     6061 CGAAAACTCA CGTTAAGGGA TTTTGGTCAT GAGATTATCA AAAAGGATCT TCACCTAGAT 
     6121 CCTTTTAAAT TAAAAATGAA GTTTTAAATC AATCTAAAGT ATATATGAGT AACCTGAGGC 
     6181 TATGGCAGGG CCTGCCGCCC CGACGTTGGC TGCGAGCCCT GGGCCTTCAC CCGAACTTGG 
     6241 GGGGTGGGGT GGGGAAAAGG AAGAAACGCG GGCGTATTGG CCCCAATGGG GTCTCGGTGG 
     6301 GGTATCGACA GAGTGCCAGC CCTGGGACCG AACCCCGCGT TTATGAACAA ACGACCCAAC 
     6361 ACCGTGCGTT TTATTCTGTC TTTTTATTGC CGTCATAGCG CGGGTTCCTT CCGGTATTGT 
     6421 CTCCTTCCGT GTTTCAGTTA GCCTCCCCCT AGGGTGGGCG AAGAACTCCA GCATGAGATC 
     6481 CCCGCGCTGG AGGATCATCC AGCCGGCGTC CCGGAAAACG ATTCCGAAGC CCAACCTTTC 
     6541 ATAGAAGGCG GCGGTGGAAT CGAAATCTCG TGATGGCAGG TTGGGCGTCG CTTGGTCGGT 
     6601 CATTTCGAAC CCCAGAGTCC CGCTCAGAAG AACTCGTCAA GAAGGCGATA GAAGGCGATG 
     6661 CGCTGCGAAT CGGGAGCGGC GATACCGTAA AGCACGAGGA AGCGGTCAGC CCATTCGCCG 
     6721 CCAAGCTCTT CAGCAATATC ACGGGTAGCC AACGCTATGT CCTGATAGCG ATCCGCCACA 
     6781 CCCAGCCGGC CACAGTCGAT GAATCCAGAA AAGCGGCCAT TTTCCACCAT GATATTCGGC 
     6841 AAGCAGGCAT CGCCATGGGT CACGACGAGA TCCTCGCCGT CGGGCATGCT CGCCTTGAGC 
     6901 CTGGCGAACA GTTCGGCTGG CGCGAGCCCC TGATGCTCTT CGTCCAGATC ATCCTGATCG 
     6961 ACAAGACCGG CTTCCATCCG AGTACGTGCT CGCTCGATGC GATGTTTCGC TTGGTGGTCG 
     7021 AATGGGCAGG TAGCCGGATC AAGCGTATGC AGCCGCCGCA TTGCATCAGC CATGATGGAT 
     7081 ACTTTCTCGG CAGGAGCAAG GTGAGATGAC AGGAGATCCT GCCCCGGCAC TTCGCCCAAT 
     7141 AGCAGCCAGT CCCTTCCCGC TTCAGTGACA ACGTCGAGCA CAGCTGCGCA AGGAACGCCC 
     7201 GTCGTGGCCA GCCACGATAG CCGCGCTGCC TCGTCTTGCA GTTCATTCAG GGCACCGGAC 
     7261 AGGTCGGTCT TGACAAAAAG AACCGGGCGC CCCTGCGCTG ACAGCCGGAA CACGGCGGCA 
     7321 TCAGAGCAGC CGATTGTCTG TTGTGCCCAG TCATAGCCGA ATAGCCTCTC CACCCAAGCG 
     7381 GCCGGAGAAC CTGCGTGCAA TCCATCTTGT TCAATCATGC GAAACGATCC TCATCCTGTC 
     7441 TCTTGATCGA TCTTTGCAAA AGCCTAGGCC TCCAAAAAAG CCTCCTCACT ACTTCTGGAA 
     7501 TAGCTCAGAG GCCGAGGCGG CCTCGGCCTC TGCATAAATA AAAAAAATTA GTCAGCCATG 
     7561 GGGCGGAGAA TGGGCGGAAC TGGGCGGAGT TAGGGGCGGG ATGGGCGGAG TTAGGGGCGG 
     7621 GACTATGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     7681 TGGGGACTTT CCACACCTGG TTGCTGACTA ATTGAGATGC ATGCTTTGCA TACTTCTGCC 
     7741 TGCTGGGGAG CCTGGGGACT TTCCACACCC TAACTGACAC ACATTCCACA GCTGGTTCTT 
     7801 TCCGCCTCAG GACTCTTCCT TTTTCAATAT TATTGAAGCA TTTATCAGGG TTATTGTCTC 
     7861 ATGAGCGGAT ACATATTTGA ATGTATTTAG AAAAATAAAC AAATAGGGGT TCCGCGCACA 
     7921 TTTCCCCGAA AAGTGCCACC TGACGCGCCC TGTAGCGGCG CATTAAGCGC GGCGGGTGTG 
     7981 GTGGTTACGC GCAGCGTGAC CGCTACACTT GCCAGCGCCC TAGCGCCCGC TCCTTTCGCT 
     8041 TTCTTCCCTT CCTTTCTCGC CACGTTCGCC GGCTTTCCCC GTCAAGCTCT AAATCGGGGG 
     8101 CTCCCTTTAG GGTTCCGATT TAGTGCTTTA CGGCACCTCG ACCCCAAAAA ACTTGATTAG 
     8161 GGTGATGGTT CACGTAGTGG GCCATCGCCC TGATAGACGG TTTTTCGCCC TTTGACGTTG 
     8221 GAGTCCACGT TCTTTAATAG TGGACTCTTG TTCCAAACTG GAACAACACT CAACCCTATC 
     8281 TCGGTCTATT CTTTTGATTT ATAAGGGATT TTGCCGATTT CGGCCTATTG GTTAAAAAAT 
     8341 GAGCTGATTT AACAAAAATT TAACGCGAAT TTT
//