LOCUS       RR212462                7959 bp    DNA     circular  SYN 
DEFINITION  Llgl2 (Myc-DDK-tagged ORF) - Rat lethal giant larvae homolog 2 (Drosophila) (Llgl2), (10 ug)
ACCESSION   NM_001127549
VERSION     NM_001127549.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4116..4207 
                     /label="Myc-DDK"  
     misc_feature    4237..4822 
                     /label="PolyA signal"  
     misc_feature    4294..4313 
                     /label="Restriction Enzyme"  
     misc_feature    5098..5770 
                     /label="ColE1"  
     misc_feature    6200..6994 
                     /label="Kan/Neo"  
     misc_feature    7029..7331 
                     /label="SV40 ori"  
     misc_feature    7417..7894 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4098..4104 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4098 
                     /label="Llgl2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAGGCGGTTC CTGAGAACTG GACATGACCC TGCCCGGGAA AGGCTAAAGC 
     1081 GGGATCTGTT CCAGTTCAAC AAGACAGTGG AGCATGGCTT CCCACACCAG CCCAGCGCCC 
     1141 TCGGCTATAG TCCTTCACTG CGCATCCTGG CCATCGGTAC CCGCTCTGGA GCTGTCAAAC 
     1201 TATATGGTGC CCCCGGGGTA GAGTTCATGG GGCTTCACAA AGAGAACAAC GCTGTGTTGC 
     1261 AGATCCACTT CCTGCCTGGT CAGTGTCAGC TGGTCACTCT GCTGGATGAC AACAGCCTGC 
     1321 ACCTGTGGAG CCTGAAGGTC AAGGGTGGGG TGTCAGAGCT GCAGGAAGAA GAGAGCTTCA 
     1381 CTTTGCGTGG CCCCCCAGGG GCGGCCCCCA GTGCCACGCA GATCACTGAG ATCCTACCTC 
     1441 ACTCCTCCGG AGAACTGCTC TACCTGGGCA CCGAGAGCGG CAGTGTGTTT GTGGTGCGGC 
     1501 TGCCTGGGTT CTGCGCACTG CACGACAGGA CCATCAGTTC AGACGAGGTG CTGCAATGGT 
     1561 TGCCAGAGGA GGCCCGGCAC CGGCGAGTGT TCGAGATGGT GGAGGCTCTG CAGGAGCACC 
     1621 CTAGAGACCC CAACCAAATC CTCATTGGCT ACAGCCGAGG CCTCGTCGTC ATCTGGGACC 
     1681 TTCGGGCCAG TCGAGTGCTC AGCCACTTCC TGAGCCACCA GCAACTGGAG AACGTCAGCT 
     1741 GGCAGCGGGA TGGCTGCCTG ATTGTCACCT GCCACTCTGA CGGCAGCCAC TGCCAGTGGT 
     1801 CCGTGCCCAG TGACACCCAG AGCCCAGAAC CTCTGCGCAG TTCTGTACCT TACGGTCCTT 
     1861 TTCCTTGCAA AGCTATCACC AAAATCTTCT GGCTTACCAC CAGGCAAGGG TTGCCCTTCA 
     1921 CCATCTTCCA GGGCGGTATG CCCCGGGCCA GCTACGGGGA CCGCCACTGC ATCTCAGTGG 
     1981 TCCACAATGG CCAGCAGACA GCCTTTGACT TCACCTCCCG TGTCATCGAC TTCACCGTTC 
     2041 TCATGGAGGC TGACCCTGTG GCTGCCTTTG ATGACCCCTA CGCCCTGGTG GTGCTTGCCG 
     2101 AGGAGGAACT GGTGGTGATT GACCTGCAGA CACCCGGCTG GCCGCCAGTC CAGCTGCCCT 
     2161 ACCTGGCTTC CCTGCACTGT TCAGCCATTA CCTGCTCCCA TCATGTCTCC AACATCCCCC 
     2221 TGAAGCTGTG GGAACGAATC ATCGCTGCCG GCAGCCGCCA GAACTCGCAC TTCTCCACCA 
     2281 TGGAGTGGCC CATAGATGGT GGTACCAGCC TGGCCCCGCC TCCACCGCAG AGGGACCTGC 
     2341 TGCTCACAGG GCACGAGGAT GGCACAGTGC GGTTCTGGGA CGCCTCGGGT GTCTGTTTAC 
     2401 GGCTGCTGTA CAAACTCAGT ACCGTGAGGG TGTTCCTTAC AGACACAGAC CTCAGCGAGA 
     2461 ACCTCAGCGC CCAGGGTGAG GACGAGTGGC CCCCACTCCG CAAGGTGGGC TCCTTTGATC 
     2521 CCTACAGTGA TGATCCACGG CTGGGCATCC AGAAGATTTT CCTCTGCAAA TACAGTGGCT 
     2581 ACCTGGCTGT GGCAGGCACG GCAGGGCAGG TGCTGGTGCT GGAGCTGAAC GATGAGGCGG 
     2641 CAGAGCACGC CGTGGAGCAG GTGGAGGCTG ACCTGCTGCA GGACCAGGAG GGCTACCGCT 
     2701 GGAAGGGCCA CGAGCGCCTC GCCGCCCGCC CCGGGCCCGT GCGCTTTGAG GCAGGCTTTC 
     2761 AGCCCTTTGT ACTGGTGCAG TGCCAGCCCC CAGCTGTGGT CACCTCCCTG GCCCTGCACT 
     2821 CTGAGTGGCG GCTTGTGGCC TTTGGCACCA GCCACGGTTT CGGCCTCTTC GATCACCAGC 
     2881 AGCGGCGGCA GGTCTTTGTC AAGTGCACAC TTCACCCCAG TGACCAGCTG GCCTTGGAGG 
     2941 GCCCGCTGTC CCGCGTAAAG TCCCTCAAGA AGTCCCTACG TCAGTCATTC CGTCGGATGC 
     3001 GCCGCAGCAG GGCGTCCAGT CATAAACGGC GGCCTGGTGG CCCCACGGGC GAGGCGCAGG 
     3061 CTCAGGCTGT GAACACCAAG GCAGAACGGA CAGGGCTGCA GAACATGGAG CTGGCACCTG 
     3121 TACAGCGCAA GATCGAGGCC CGCTCAGCAG AGGACTCCTT CACCGGCTTT GTTCGGACCC 
     3181 TCTATTTTGC TGACACCTAC CTGAGGGACA GTTCCCGCCA CTGTCCCTCG CTGTGGGCTG 
     3241 GCACCAATGG AGGCACCGTC TATGCCTTCT CCCTGCGCGT ACCTCCTGCA GAAAGGAGAG 
     3301 CGGATGAGCC TGTGCGGGCA GAGCAGGCCA AGGAGATCCA GCTGATGCAT CGTGCACCTG 
     3361 TGGTGGGCAT CCTGGTGCTT GACGGACACA ACGTACCCCT TCCCGAGCCC CTGGAAGTAG 
     3421 CCCATGACCT GTCCAAGAGC CCAGACATGC AAGGAAGCCA CCAGCTGCTT GTGGTGTCAG 
     3481 AGGAGCAATT CAAGGTGTTC ACGCTGCCCA AGGTGAGCGC CAAGCTGAAG CTGAAGCTGA 
     3541 CGGCCCTGGA AGGCTCGCGG GTTCGACGAG TCGGGGTTGC CCACTTCGGC AGCTGCAGGG 
     3601 CGGAGGACTA TGGGGAGCAC CACCTGGCAG TGCTCACCAA TTTGGGTGAC ATCCAAGTGG 
     3661 TCTCAGTGCC CCTGCTCAAG CCCCAGGTGC GGTACAGCTG CATCCGCCGG GAGGATGTCA 
     3721 GTGGAATAGC CTCCTGCGTC TTCACCAAGT ACGGCCAAGG CTTCTACCTG ATATCACCTT 
     3781 CGGAGTTCGA GCGCTTTTCT CTCTCCACCA AGTGGCTGGT TGAGCCCCGG TGTTTGGTGG 
     3841 ATTCAGCCAA AGCCAGGAAC CACAACCGGC CCAGTAATGG CAATAGCACA GGCCCCAAAA 
     3901 GGACCTCCGG CCAAGTCAGG AACTCACGGA GCCAGAGTGA TGGAGCAGAG ACGAAGCCCG 
     3961 GCCCAGTGAT GGAGCACGCA CTGCTCAGTG ACGCCTGGGT CCTAAAGGAA GTCCAGAGCA 
     4021 CGCTAGAGGG GGACCAGGGG AGCTGTGGCA ATTGGCGCTC TCACCGTGTG GCTGTGGGTT 
     4081 GCAGGCTGAG CAATGGCGAA GCAGAGACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4141 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4201 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4261 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4321 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4381 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4441 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4501 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4561 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4621 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     4681 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     4741 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     4801 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     4861 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     4921 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     4981 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5041 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5101 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5161 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5221 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5281 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5341 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5401 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5461 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5521 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5581 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     5641 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     5701 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     5761 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     5821 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     5881 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     5941 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6001 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6061 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6121 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6181 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6241 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6301 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6361 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6421 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6481 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6541 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6601 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     6661 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     6721 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     6781 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     6841 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     6901 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     6961 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7021 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7081 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7141 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7201 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7261 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7321 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7381 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7441 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7501 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7561 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7621 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     7681 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     7741 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     7801 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     7861 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     7921 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//