LOCUS       RR212422                8319 bp    DNA     circular  SYN 
DEFINITION  Arhgap20 (Myc-DDK-tagged ORF) - Rat Rho GTPase activating protein 20 (Arhgap20), (10 ug)
ACCESSION   NM_213629
VERSION     NM_213629.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4476..4567 
                     /label="Myc-DDK"  
     misc_feature    4597..5182 
                     /label="PolyA signal"  
     misc_feature    4654..4673 
                     /label="Restriction Enzyme"  
     misc_feature    5458..6130 
                     /label="ColE1"  
     misc_feature    6560..7354 
                     /label="Kan/Neo"  
     misc_feature    7389..7691 
                     /label="SV40 ori"  
     misc_feature    7777..8254 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4458..4464 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4458 
                     /label="Arhgap20"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAAACGCTA GCAGAAAGGA GGAGAAGCGC CCCATCCCTC ATCCTGGACA 
     1081 AAGCCTTACA GAAGCGGCCG AGCACCAGAG ACAGCCATTC TGCCAGCATT GACACGTGTG 
     1141 CGTTTCTGTC GTCGTTCATG TGCTCCAGTC GGACTCTGCT GATTGATGGT CCAGTGGAAC 
     1201 TTAAACGAGG CCTGCAGAGG CAGGAGAGGC ATCTTTTCCT ATTCAACGAT CTGTTTGTCT 
     1261 CGGCCAAAAT CAAATATAAC AACAATTTTA AGATAAAGAA TAAAGTAAAG CTGGCCGACA 
     1321 TGTGGACAGC TAGCTGTGTG GATGAAGTCG GGGAAGGCAA CACAAACGCT CTGAAGTCCT 
     1381 TTGTCCTGGG CTGGCCCACA GTGAACTTCG TGGCCACTTT CAGTTCTCCA GAACAAAAGG 
     1441 ACAAATGGCT CTCTCTCCTT CAGAGATACA TCGCTCTAGA AAAGGAAAAA GACTACCCGA 
     1501 AGAGCATCCC CCTCAAAATC TTCGCCAAGG ACATTGGGAA TTGCGCCTAT TTCAAAACTA 
     1561 TATCAGTCAT GAACTCAGAC ACAGCGAGTG AAGTCATCAA CATGTCCCTG CAAATGTTAG 
     1621 GGATAACTGG CTCCGAGAGA GATTACCAAC TATGGGTAAA TTCTGGAAAA GAAGCGGCAC 
     1681 CATACCCACT CATTGGACAC GAGTATCCTT ATGGGATCAA AATGAGCCAT CTCCGAGACA 
     1741 CTGCCCTTCT GACGCAGGGA TCAAAAGACT TCGCCAGCCC ATCCCAGCTC CAGGAGCCTT 
     1801 TCCTCATGGA GCAGCTGCCC CGAGAGATGC AATGCCAGTT CATCCTGAAA CCTACTCGCC 
     1861 TGGCGACAGC CCAGCAGCTG AGCGATTCCA GCCATAAGAC ATATAAGAGG AGGCGCTCCA 
     1921 TCATCAACTG GGCTTTCTGG CGGGGCTCTA GCACACACCT GGACAATCTG CCCGTGTCAC 
     1981 CAACATCCCC TATGCCAGGG CAGCTCTTTG GAGTTTCTTT ACCAGATATT TGCGAGAACG 
     2041 ACAATCTGCC AAAGCCCATC TTGGACATGC TCTCCTTTCT TAACCAAAAA GGCCCCCTCA 
     2101 CGAAGGGCAT CTTCAGGCAG TCGGCCAACA TGAAGTCTTG CCGAGAGCTA AAGGAGAAAC 
     2161 TGAATTCTGG AATTGAAGTC CACCTCGACT GTGAATCCAT CTTTGTGATA GCATCCGTGT 
     2221 TGAAGGATTT TCTGAGAAAT ATCCCAGAAA GTATATTTTC ATCAGATCTG TATGATCACT 
     2281 GGGTCTGTGT AATGGATCAA GGAAATGATG AAGAAAAAAT AAATACAATT CAAAGGCTAT 
     2341 TAGATCAGCT TCCCAGAGCC AATGTTGTTT TCCTAAGGTA TCTGTTTGGG GTATTACACA 
     2401 ACATTGAACA ACATTCCTTA TCTAATCAGA TGACTGCATT TAACTTAGCA GTGTGTATCG 
     2461 CTCCAAGCAT TCTTTGGCCT CCTGCTTCCT CCAGTCCAGA GCTAGAAAAT GAATTTACAA 
     2521 AAAAGGTTTC TCTCCTTATA CAATTTCTGA TTGAAAATTG CTGTAGGATA TTTGGAGAAG 
     2581 AAATCACTTC TCTCTTGGGG GAGCTTTCTG AGAGAAGCGA CAGAGAACAC ACCCCAGATA 
     2641 CGTCTTGCTT CCAGTTGAAC GACTCCTCCT ATGACAGCTT AGAAAACGAG CTCAATGAGG 
     2701 ATGCCGATGC CCCGTGCAGC GACCTGGTCA AACGGCTCGG CCAGGGTAGC AGGAGCATGG 
     2761 ACCCAGTCTT AACCCTCAGT GACTATGACC TTGAACCGCC CGAAGCGGAA GGCCTTTTAA 
     2821 CCCTGAGCAG CCTTGACTTA GATCAGTCTA AAGAGGAGCA CATTCGAACG AAACAACCCC 
     2881 TGGAGACCAA GCCGGTGAGT GTTTTCGTAG CCTACAGGAA GGTTTCACTG GGGGAGCATA 
     2941 CTAGGGCCCC CGATGGCCCC GGTACACCCA GCTGCCTGCC TGCAACTGCC TCAGATGCCC 
     3001 GGAAAGTCTT TCGGCGGCAC CGGCGCTCCT CAGAGCCCAG CATTGACTAT CTAGACGCAA 
     3061 AGCTTTCCTA TCTCCGGGAG TTTTACCAGA AGAAGTTACG CAAGTCCAGC TGTGATGCCG 
     3121 TGCTCTCTAG AAAGGATGAG GATTACCTGA AGCAGACCCA GCCTCAGAAG AAAGGGGATC 
     3181 AGAGGTGTTT TAAACAGAGC TCAGTGACGG GCACTGACGT CAGCAAGAGG AACACTGCTA 
     3241 ATGAAAACAT TAAGAAGAAA AGCTTGTCTG GTCATGAAGG AAATCAAGAG ACACCTTTCA 
     3301 CTAAGTCCAA GCCAGTGGCC ATTTCTGTGG CATCTTACAT GTCCTCCCAG GACCATTCCT 
     3361 GGGAGCAGCC CTTTGAAGCA GACGCATGCA GGTTCTCCCC ACCACACATA GCAGATGCCC 
     3421 AGAAGAGCTC ACGGGCGCAT CGGCGCTGTT CCGAACCCAG CATCGATGAC CAGAACTACA 
     3481 AGCTGTCCTA TCTCAGGGGG GTCTACTCAA AGAAGCAAAG TAAAACCAGC TGTGAGGCTG 
     3541 GCCTCTTGCA TGGCGAAGAG GATTATCTCA AAAGGCATAA GTCTTTGCAA ATGGAGGGGC 
     3601 AAAAGCTCAT TAATCAGAGT TTGGTCATGG GGATTGAGGT GGGCAAGAGC AGCAGCAGCA 
     3661 CAAACCAAAG TACTGAGAAG GTTTTGCCCC CAAGACTAAA CCTTTGCCCA AGGGCTAGCT 
     3721 ATTCCAGTTT ATCTTCCCCC GGCACATCCC CGTCAGGTTC CTCGGTAAGC TCCCAGGACA 
     3781 GTGCTTTCTC TCAGATCTGT GAACACTCGG TGTTTACACC CACAGAAACT TCCTCTCCAA 
     3841 TAGATTGCAC TTTTCGGGCT CAGAGAAAAC AGGAAGAGCT TTCTTCAGAC TGTGACAGTC 
     3901 CCAGCCTCGT TTCTGGAATG CCGGGTCCCT CCATGGGGCA GGCCAGCAGC CACCTAGCTT 
     3961 ATTTAAGAAA GGGTACCACA GAGCAGCTAC CACAAATGCA TTCTGTAACT CTTCACCCCA 
     4021 GCACATGGTT GAGAAGCGGC TTGGTCACTT TAAAAAACTG GTCCCTCAAA AAGAAAACAA 
     4081 AAGCAGCCAG ACCCGAGGAC AGAAAAGACT GTTCCTTAAA AGAACCTCTG GAATTACCTG 
     4141 CATGTGCTGC TGGGACCCCA GAGGCTGACT CTCTACAAGA GAGCCAGGAG GACATACACC 
     4201 TAGGGGTAGA TGAAGAAGCT GGGCAAACTA CCTGTGGGCT CAGCTCTTAT GCCTGTCAGG 
     4261 ACTCAGAGCA ACACGCCAGC TCCCCGTTCT GCCTGGCAGG AAGCAGACTC ACGCTTGGTA 
     4321 TCAAGTTGCA CGAGGGAGAG GAGAGTGGAG GGCAGTACCC TTGTGATAAC CCTTGGGAAG 
     4381 GAGCCACTTC AGGTCTGGAG ACCTCAGAGG ATGCAGCCAA CACAGGTGTG GAACCTGCGA 
     4441 CAGTTTGCAA TGACGGGGAC AGACATACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4501 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4561 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4621 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4681 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4741 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4801 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4861 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4921 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     4981 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5041 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5101 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5161 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5221 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5281 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5341 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5401 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5461 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5521 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5581 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5641 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5701 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5761 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5821 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5881 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     5941 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6001 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6061 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6121 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6181 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6241 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6301 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6361 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6421 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6481 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6541 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6601 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6661 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6721 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6781 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6841 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6901 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     6961 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7021 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7081 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7141 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7201 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7261 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7321 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7381 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7441 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7501 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7561 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7621 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7681 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7741 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7801 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7861 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7921 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     7981 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8041 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8101 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8161 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8221 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8281 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//