LOCUS       RR212340                7722 bp    DNA     circular  SYN 
DEFINITION  Pcdha4 (Myc-DDK-tagged ORF) - Rat protocadherin alpha 4 (Pcdha4), (10 ug)
ACCESSION   NM_053933
VERSION     NM_053933.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3879..3970 
                     /label="Myc-DDK"  
     misc_feature    4000..4585 
                     /label="PolyA signal"  
     misc_feature    4057..4076 
                     /label="Restriction Enzyme"  
     misc_feature    4861..5533 
                     /label="ColE1"  
     misc_feature    5963..6757 
                     /label="Kan/Neo"  
     misc_feature    6792..7094 
                     /label="SV40 ori"  
     misc_feature    7180..7657 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3861..3867 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3861 
                     /label="Pcdha4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAATTTTCC TGGGGAAGTG GCCAGGAATC CCAGCGCCTG CTTCTCTCTT 
     1081 TTCTGTTTCT TGCAATCTGG GAGCCAGGAA ACAGCCAGCT CCACTACTCC ATCCCAGAGG 
     1141 AGGCCAAACA CGGCACCTTC GTGGGCCGCA TCGCACAGGA CCTGGGGCTG GAGCTGGCGG 
     1201 AGCTGGTGCC TCGCCTGTTC AGGGTGGCGT CCAAGGACCG CGGAGACCTT CTGGAGGTAA 
     1261 ATCTGCAGAA TGGCATTTTG TTTGTGAATT CTCGGATCGA TCGGGAGGAG CTGTGCGGGA 
     1321 GGAGTGCGGA GTGTAGCATC CACTTGGAGG TGATCGTGGA CAGGCCGCTG CAGGTTTTCC 
     1381 ACGTGGAGGT AGAGGTGAGG GACATTAACG ACAACCCTCC CACGTTCCCA ACCACACAAA 
     1441 AGAATCTGTT CATCGCAGAA TCAAGGCCAC TTGACACTTG GTTTCCACTA GAGGGCGCTT 
     1501 CAGATTCAGA TATCGGAATC AATGCTGTGC TGACTTACAG ACTAAGTCCA AATGATTACT 
     1561 TTTCTTTGGA AAAACCAACC AACAGTGAAC GGGTAAAAGG TCTTGGGCTT ATATTACGGA 
     1621 AATCTTTAGA CCGGGAGGAA ACTCCAGAGT TATTTTTAGT GCTCACTGTC ACGGATGGAG 
     1681 GAAAGCCCGA GCTGACTGGC AGTGTTCAGT TACTCATCAC AGTGCTGGAT GCCAACGATA 
     1741 ATTCTCCAGT TTTTGACAGA TCTCTCTATA CCGTGAAATT ACCAGAAAAC GTCCCAAATG 
     1801 GGACATTGGT AGTCAAAGTC AATGCCTCAG ATTTAGATGA AGGCGCCAAT GGGGAAGTTA 
     1861 TGTATTCATT TTCTACAGAT ATTTCACCAA ATGTGAAAAA CAAATTCCAC ATAGACCCGG 
     1921 TTACCGGAGA GATCGCTGTA AAGGGATACA TTGATTTTGA AGAATGCACA TCCTATGAAA 
     1981 TTCTCATAGA GGGAATTGAC AAGGGACAAC TTCCCCTCTC TGGACACTGT AAAGTTATTG 
     2041 TACAAGTTGA AGACATCAAT GATAACGCCC CAGAGTTGGA ATTCAAATCT CTATCACTTC 
     2101 CAATCAGAGA GAATGCTCCG GTGGGAACTG TCATCGCACT CATCAGTGTG TTTGATCCGG 
     2161 ACACGGGTGT CAATGGGCAG GTTACTTGCT CCCTGACTCC TCAGGTCCCC TTCAAGCTGG 
     2221 TGTCCACTTT CAAGAATTAC TATTCGCTTG TGTTGGACAG CGCCCTGGAC CGGGAGACCA 
     2281 CAGCTGACTA TAAGGTGGTA GTGATAGCCC GGGATGGGGG CTTGCCCTCG CTGTGGGCCA 
     2341 CGGCCAGTGT GTCCGTGGAG GTGGCTGACG TGAACGACAA TGCCCCTGCG TTCGCACAGC 
     2401 CTGAATACAC GGTGTTTGTG AAGGAGAACA ACCCACCTGG AGCGCACATC TTCACGGTAT 
     2461 CCGCGGTGGA CGCAGACTCA CAGGAGAACG CGCTGGTGTC CTACTCGCTG GTGGAGCGGC 
     2521 GGGTGGGCGA GCGCTTGCTG TCAAGCTATG TCTCTGTGCA CGCGGAGAGC GGCAAGGTGT 
     2581 TCGCACTGCA GCCTCTGGAC CATGAGGAGC TGGAGCTGCT GCAGTTCCAG GTGAGCGCGC 
     2641 GGGATGCTGG TGTGCCTGCC CTGGGCAGCA ATGTGACTCT GCAGGTGTTT GTGCTGGATG 
     2701 AGAACGACAA CGCGCCCACC CTGCTGGAAC CCGAGGCAGG AATCTCTGGT GGAATCGTGA 
     2761 GCCGGCTAGT GTCCAGATCA GTAGGTGCGG GCCATGTGGT GGCCAAAGTG CGCGCGGTGG 
     2821 ATGCGGACTC TGGCTATAAT GCATGGCTCT CTTATGAGCT GCAATCGTCA GAAGGCAATT 
     2881 CCCGTAGCCT ATTCCGTGTG GGTCTATATA CGGGCGAGAT TAGTACTACG CGCAGCTTGG 
     2941 ATGAAGCAGA TTCGCCGCGT CAGCGCCTTC TGGTGCTGGT GAAGGACCAT GGCGACCCAG 
     3001 CAATGATGGT TACTGCCACA GTGTTGGTGT CGCTGGTAGA GAATGGCCCG CTACCAAAGG 
     3061 CTCCATCGCG AGTGTCCACA AGAGTCACAA ACGCGGAGGC GTCACTGGTG GATGTCAACG 
     3121 TGTATCTGAT CATCGCCATC TGTGCAGTAT CCAGCCTGTT AGTGCTCACG CTGCTGCTGT 
     3181 ACAGTGCCCT GCGCTGCTCC ACTGTCCCCA GTGAGAGCGT GTGCGGGCCT CCAAAACCAG 
     3241 TAATGGTGTG CTCCAGCGCA GTGGGGAGCT GGTCATACTC TCAGCAAAGG AGGCAAAGGG 
     3301 TGTGCTCTGG AGAGTACCCA CCTAAGACCG ACCTCATGGC CTTTAGTCCC AGTCTATCTG 
     3361 ATTCAAGGGA CAGAGAGGAT CAATTGCAGT CCACAGAGGA TTCCTCTGGA AAGCCCCGAC 
     3421 AGCCCAACCC TGACTGGCGC TACTCTGCCT CGCTAAGAGC CGGCATGCAC AGCTCTGTGC 
     3481 ACCTGGAGGA GGCTGGCATT CTACGGGCTG GTCCAGGAGG GCCTGATCAA CAGTGGCCAA 
     3541 CAGTATCCAG TGCAACACCA GAACCAGAGG CAGGAGAGGT GTCCCCTCCA GTGGGCGCTG 
     3601 GTGTCAACAG CAACAGCTGG ACCTTTAAAT ACGGACCCGG CAACCCCAAA CAGCCCGGTC 
     3661 CCGGTGAGTT GCCAGACAAA TTCATTATCC CAGGATCTCC TGCAATCATC TCCATCCGGC 
     3721 AGGAGTCTGC TAACAACCAA ATTGACAAAA GCGATTTTAT AACCTTCGGC AAAAAGGAGG 
     3781 AGACCAAGAA AAAGAAGAAA AAGAAGAAGG GTAACAAGAC CCAGGAGAAA AAAGAGAAAG 
     3841 GGAACAGCAC GACGGACAAC AGTGACCAGA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     3901 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     3961 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4021 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4081 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4141 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4201 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4261 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4321 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4381 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     4441 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     4501 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     4561 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     4621 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     4681 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     4741 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     4801 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     4861 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     4921 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     4981 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5041 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5101 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5161 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5221 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5281 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5341 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     5401 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     5461 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     5521 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     5581 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     5641 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     5701 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     5761 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     5821 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     5881 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     5941 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6001 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6061 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6121 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6181 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6241 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6301 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6361 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     6421 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     6481 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     6541 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     6601 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     6661 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     6721 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     6781 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     6841 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     6901 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     6961 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7021 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7081 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7141 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7201 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7261 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7321 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7381 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     7441 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     7501 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     7561 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     7621 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     7681 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//