LOCUS       RR212309                5971 bp    DNA     circular  SYN 
DEFINITION  Gpr139 (Myc-DDK-tagged ORF) - Rat G protein-coupled receptor 139 (Gpr139), (10 ug)
ACCESSION   NM_001024241
VERSION     NM_001024241.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    2128..2219 
                     /label="Myc-DDK"  
     misc_feature    2249..2834 
                     /label="PolyA signal"  
     misc_feature    2306..2325 
                     /label="Restriction Enzyme"  
     misc_feature    3110..3782 
                     /label="ColE1"  
     misc_feature    4212..5006 
                     /label="Kan/Neo"  
     misc_feature    5041..5343 
                     /label="SV40 ori"  
     misc_feature    5429..5906 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    2110..2116 
                     /label="Restriction Enzyme"  
     misc_feature    1020..2110 
                     /label="Gpr139"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGAGCACACG CACGCCCACC TCGCTGCGAA TAGCTCAGCT TGCGGCTTGG 
     1081 GCTTCGTGCC GGTGGTCTAC TACAGCTTCT TGTTGTGCCT CGGGTTACCA GCAAATATCT 
     1141 TGACAGTCAT CATCCTCTCT CAACTGGTAG CCAGAAGACA GAAGTCCTCC TACAACTATC 
     1201 TTCTGGCACT TGCTGCTGCC GACATCTTGG TCCTCTTTTT CATCGTCTTT GTGGATTTCT 
     1261 TGTTAGAAGA CTTCATTTTG ACCATGCAGA TGCCTCCGAT CCCTGACAAG ATCATAGAAG 
     1321 TGCTAGAGTT CTCCTCCATC CACACTTCTA TTTGGATTAC AGTCCCCTTA ACAGTTGATA 
     1381 GGTATATCGC AGTCTGTCAC CCACTCAAAT ACCACACAGT TTCCTACCCA GCCAGGACCC 
     1441 GGAAAGTCAT TCTGAGTGTT TATATTACCT GCTTCCTGAC TAGCATCCCC TACTACTGGT 
     1501 GGCCTAACAT CTGGACTGAA GACTACATCA GTACCTCCAT GCATCATGTC CTTGTCTGGA 
     1561 TCCACTGCTT CACCGTGTAC CTGGTGCCCT GCTCCATCTT CTTCATCTTG AACTCAATCA 
     1621 TTGTGTACAA GCTTAGGAGA AAGAGTAATT TCCGCCTCCG TGGCTATTCC ACTGGCAAGA 
     1681 CCACTGCCAT CTTGTTTACC ATCACCTCCA TCTTTGCTAC CCTCTGGGCC CCCCGCATCA 
     1741 TCATGATTCT CTACCACCTC TACGGAGCAC CCATCCAGAA CCCTTGGCTG GTCCACATTA 
     1801 TGTTGGATGT TGCCAACATG CTGGCTCTTC TGAACACAGC CATCAACTTC TTCCTCTACT 
     1861 GCTTCATCAG CAAGCGCTTC CGTACCATGG CAGCTGCTAC ACTCAAGGCC TTGTTCAAGT 
     1921 GCCAGAAGCA GCCTGTACAG TTCTACACCA ACCATAACTT TTCCATAACA AGTAGTCCCT 
     1981 GGATCTCACC AGCAAACTCA CACTGCATCA AAATGTTGGT GTACCAGTAT GACAAACATG 
     2041 GGAAGCCTAT AAAAGTATCC CCGCAAAATG TTGGTGTACC AGTATGACAA ACATGGGAAG 
     2101 CCTATAAAAG TATCCCCGAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG 
     2161 GATCTGGCAG CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC 
     2221 GGCCGCGGTC ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC 
     2281 AGTGCCTCTC CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA 
     2341 ATTAAGTTGC ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG 
     2401 GGTGGTATGG AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG 
     2461 GGAACCAAGC TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT 
     2521 TCAAGCGATT CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG 
     2581 GCTCAGCTAA TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT 
     2641 CTCCAACTCC TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA 
     2701 GGCGTGAACC ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA 
     2761 GGACGTCCAG ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG 
     2821 CTTGCTTGGC ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG 
     2881 TACGCGGCCA GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG 
     2941 AATCAGGGGA TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC 
     3001 GTAAAAAGGC CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA 
     3061 AAAATCGACG CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT 
     3121 TTCCCCCTGG AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC 
     3181 TGTCCGCCTT TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC 
     3241 TCAGTTCGGT GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC 
     3301 CCGACCGCTG CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT 
     3361 TATCGCCACT GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG 
     3421 CTACAGAGTT CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA 
     3481 TCTGCGCTCT GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA 
     3541 AACAAACCAC CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA 
     3601 AAAAAGGATC TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG 
     3661 AAAACTCACG TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC 
     3721 TTTTAAATTA AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA 
     3781 TGGCAGGGCC TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG 
     3841 GGTGGGGTGG GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG 
     3901 TATCGACAGA GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC 
     3961 CGTGCGTTTT ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT 
     4021 CCTTCCGTGT TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC 
     4081 CGCGCTGGAG GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT 
     4141 AGAAGGCGGC GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA 
     4201 TTTCGAACCC CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG 
     4261 CTGCGAATCG GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC 
     4321 AAGCTCTTCA GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC 
     4381 CAGCCGGCCA CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA 
     4441 GCAGGCATCG CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT 
     4501 GGCGAACAGT TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC 
     4561 AAGACCGGCT TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA 
     4621 TGGGCAGGTA GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC 
     4681 TTTCTCGGCA GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG 
     4741 CAGCCAGTCC CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT 
     4801 CGTGGCCAGC CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG 
     4861 GTCGGTCTTG ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC 
     4921 AGAGCAGCCG ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC 
     4981 CGGAGAACCT GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC 
     5041 TTGATCGATC TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA 
     5101 GCTCAGAGGC CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG 
     5161 GCGGAGAATG GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA 
     5221 CTATGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG 
     5281 GGGACTTTCC ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG 
     5341 CTGGGGAGCC TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC 
     5401 CGCCTCAGGA CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT 
     5461 GAGCGGATAC ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT 
     5521 TCCCCGAAAA GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT 
     5581 GGTTACGCGC AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT 
     5641 CTTCCCTTCC TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT 
     5701 CCCTTTAGGG TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG 
     5761 TGATGGTTCA CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA 
     5821 GTCCACGTTC TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC 
     5881 GGTCTATTCT TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA 
     5941 GCTGATTTAA CAAAAATTTA ACGCGAATTT T
//