LOCUS       RR212295                7476 bp    DNA     circular  SYN 
DEFINITION  Med16 (Myc-DDK-tagged ORF) - Rat mediator complex subunit 16 (Med16), (10 ug)
ACCESSION   NM_001106768
VERSION     NM_001106768.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3633..3724 
                     /label="Myc-DDK"  
     misc_feature    3754..4339 
                     /label="PolyA signal"  
     misc_feature    3811..3830 
                     /label="Restriction Enzyme"  
     misc_feature    4615..5287 
                     /label="ColE1"  
     misc_feature    5717..6511 
                     /label="Kan/Neo"  
     misc_feature    6546..6848 
                     /label="SV40 ori"  
     misc_feature    6934..7411 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3615..3621 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3615 
                     /label="Med16"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGCGCCG GATCCCGGGC GTTGGGGCGA GTGTGGACCG CGGCCGCCCG 
     1081 GTCGGCTGGG CGCCCGGGGC GGACAGGAGC CAGGGACGGT GCTGGGTGCG GTGGGTATGA 
     1141 TGGACCTAGC CTATGTGTGC GAATGGGAGA AGTGGGCCAA GAGCACGTAC TGCCCGTCGC 
     1201 TGCCCCTGGC CTGCGCCTGG TCCTGCAGGA ACCTCATTGC CTTCACCACA GACCTACGCA 
     1261 ATGATGACCA GGACCTCACA CACATGATTC ATATCCTTGA CACAGAACAT CCCTGGGAAG 
     1321 TGCACTCTGT CAGCTCTGGC CACAGTGAGG CCATCACCTG CCTGGAATGG GACCAGTCAG 
     1381 GGTCCCGGCT CCTGTCTGCC GATGCTGACG GGCAGATCAA ATGTTGGAGC ATGGCTGACC 
     1441 ACCTGGCCAA CAGCTGGGAG AGCTCCGTGG GCAGCCAGGT GGAAGGGGAC CCCATCGTGG 
     1501 CTCTGTCCTG GCTGCATAAC GGGGTGAAAC TGGCACTGCA CGTAGAGAAG TCTGGTGCCT 
     1561 CCAGCTTCGG AGAAAAGTTC TCCCGGGTGA AATTCTCCCC GTCTCTCACG CTGTTTGGTG 
     1621 GCAAGCCAAT GGAGGGTTGG ATTGCGGTGA CAGTCAGCGG CCTGGTCACT GTGTCCCTCC 
     1681 TGAAGCCCAG TGGGCAGGTG CTGACGTCCA CGGAGAGCCT GTGCCGGCTG CGTGGCCGAG 
     1741 TGGCACTGGC CGACATCGCC TTCACAGGCG GTGGGAACAT CGTTGTGGCC GCTGCGGACG 
     1801 GCAGCAGTGC ATCGCCCGTG AAGTTCTACA AGGTGTGCGT GAGCGTGGTG AGCGAGAAGT 
     1861 GCCGCATCGA CACGGAGATC CTGCCCTCGC TGTTTATGCG CTGCACCACC GACCCCAACC 
     1921 GCAAGGACCG GTTCCCCGCC ATCACACACC TCAAGTTCCT GGCGAGAGAC ATGTCTGAGC 
     1981 AGGTGCTCCT GTGTGCATCC AGCCAGACCA GCAGCCTAGT GGAGTGCTGG TCCCTTCGGA 
     2041 AAGAGGGCCT TCCTGTGAAC AACATCTTCC AGCAGATCTC GCCTGTGGTT GGTGACAAAC 
     2101 AGCCCATGAT CCTCAAGTGG CGGATCCTGT CAGCCACCAA TGACCTGGAC CGTGTATCAG 
     2161 CTGTGGCACT GCCGAAACTG CCCATCTCAC TCACCAACAC TGACCTCAAG GTGGCCAGTG 
     2221 ACACCCAGTT CTATCCAGGC CTTGGCTTGG CGCTGGCCTT CCAGGACGGC AGCGTGCACA 
     2281 TGGTGCATCG GCTATCCCTT CAGACCATGG CTGTATTCTA CAGCTCGGCC CCACGCTCTC 
     2341 TGGATGAGCC TGCCCTGAAG CGCCCACGCA CCACAGGCCC CGCTGTACAC TTCAAGGCCA 
     2401 TGCAGCTGTC CTGGACTTCG CTGGCCCTGG TGGGCATCGA TAACCACGGG AAGCTCAGCA 
     2461 TGCTGCGCAT CTCTCCGTCC CTGGGCCACG CGCTGGAGCC CAAGCTGGCC CTGCAGCATC 
     2521 TGCTGTTCCT GCTGGAGTAC TGCATGGTGA CTGGCTACGA CTGGTGGGAC ATCCTGCTCC 
     2581 ACGTGCAGCC AGGCATGGTG CAGAGCCTGG TGGAGCGGCT GCACGAGGAG TACACACGCC 
     2641 AGAAGCCTGC CCTGCAACAG GTCCTCTCCA CCCGGATCTT AGCCATGAAG GCCTCGCTGT 
     2701 GCAAGCTGTC ACCCTGCACC GTGGCTCGTG TGTGTGACTA CCACACCAAG CTGTTCCTCA 
     2761 TGGCTATCAC GTCCACCCTC AAGTCGCTGC TGCGCCCACA CTTCCTCAAC ACCCCTGACA 
     2821 AGAGCCCTGG AGACCGCCTG GCCGAGATCT GTGCCAAGAT CACCGATGTT GACATCGACA 
     2881 AAGTCATGAT CAACCTGAAG ACAGAAGAGT TTGTTCTAGA CATGAACACT TTGCAGGCGC 
     2941 TGCAGCAGTT GCTACAATGG GTGGGAGACT TCGTGCTCTA CCTCCTGGTC AGCCTGCCCA 
     3001 ACCAGGGCTC CGCACTGAGG CCGGGCCACA GCTTCCTCCG GGATGGTGCC TCACTGGGTA 
     3061 TGCTGCGGGA ATTGATGGTT GTCATCCGAA TATGGGGCCT ACTGAAGCCC AGTTGCCTGC 
     3121 CCGTCTATAC AGCCACCTCA GACACCCAGG ACAGCATGTC CCTGCTCTTC CGGCTGCTCA 
     3181 CCAAGCTGTG GATTTGCTGC CGTGACGAAG GCCCAGCCAG TGAGCCCGAT GAGGGCTTGG 
     3241 TGGACGAATG CTGCCTGCTG CCCAGCCAGC TGCTGGTCCC CAGCCTGGAC TGGCTTCCTG 
     3301 CCAGTGACGG CCTGGTCAGT CGCCTGCAGC CCAAACAGCC CTTGCGCCTG CGCTTCGGCA 
     3361 GGGCACCCAC CCTGCCCAGC AGCACCTCCA TCCTGCAGCT GGACGGCCTC ACCAGGGCCC 
     3421 CTGGGCAGCC CAAGATCGAC CACCTTCGGA GGCTACACCT GGGTGCCTAC CCCACGGAGG 
     3481 AGTGCAAAGC CTGCACCAGG TGTGGCTGTG TCACCATGCT GAAGTCACCC AACAAGACAA 
     3541 CTGCTGTGAA ACAGTGGGAA CAGCGGTGGA TCAAGAATTG CCTGTGTGGT GGGCTGTGGC 
     3601 GGAGAGTGCC TCTCAGCTGT TCCACGCGTA CGCGGCCGCT CGAGCAGAAA CTCATCTCAG 
     3661 AAGAGGATCT GGCAGCAAAT GATATCCTGG ATTACAAGGA TGACGACGAT AAGGTTTAAA 
     3721 CGGCCGGCCG CGGTCATAGC TGTTTCCTGA ACAGATCCCG GGTGGCATCC CTGTGACCCC 
     3781 TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT GCCACTCCAG TGCCCACCAG CCTTGTCCTA 
     3841 ATAAAATTAA GTTGCATCAT TTTGTCTGAC TAGGTGTCCT TCTATAATAT TATGGGGTGG 
     3901 AGGGGGGTGG TATGGAGCAA GGGGCAAGTT GGGAAGACAA CCTGTAGGGC CTGCGGGGTC 
     3961 TATTGGGAAC CAAGCTGGAG TGCAGTGGCA CAATCTTGGC TCACTGCAAT CTCCGCCTCC 
     4021 TGGGTTCAAG CGATTCTCCT GCCTCAGCCT CCCGAGTTGT TGGGATTCCA GGCATGCATG 
     4081 ACCAGGCTCA GCTAATTTTT GTTTTTTTGG TAGAGACGGG GTTTCACCAT ATTGGCCAGG 
     4141 CTGGTCTCCA ACTCCTAATC TCAGGTGATC TACCCACCTT GGCCTCCCAA ATTGCTGGGA 
     4201 TTACAGGCGT GAACCACTGC TCCCTTCCCT GTCCTTCTGA TTTTAAAATA ACTATACCAG 
     4261 CAGGAGGACG TCCAGACACA GCATAGGCTA CCTGGCCATG CCCAACCGGT GGGACATTTG 
     4321 AGTTGCTTGC TTGGCACTGT CCTCTCATGC GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA 
     4381 TTGGGTACGC GGCCAGCGGC GAGCGGTATC AGCTCACTCA AAGGCGGTAA TACGGTTATC 
     4441 CACAGAATCA GGGGATAACG CAGGAAAGAA CATGTGAGCA AAAGGCCAGC AAAAGGCCAG 
     4501 GAACCGTAAA AAGGCCGCGT TGCTGGCGTT TTTCCATAGG CTCCGCCCCC CTGACGAGCA 
     4561 TCACAAAAAT CGACGCTCAA GTCAGAGGTG GCGAAACCCG ACAGGACTAT AAAGATACCA 
     4621 GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG CTCTCCTGTT CCGACCCTGC CGCTTACCGG 
     4681 ATACCTGTCC GCCTTTCTCC CTTCGGGAAG CGTGGCGCTT TCTCATAGCT CACGCTGTAG 
     4741 GTATCTCAGT TCGGTGTAGG TCGTTCGCTC CAAGCTGGGC TGTGTGCACG AACCCCCCGT 
     4801 TCAGCCCGAC CGCTGCGCCT TATCCGGTAA CTATCGTCTT GAGTCCAACC CGGTAAGACA 
     4861 CGACTTATCG CCACTGGCAG CAGCCACTGG TAACAGGATT AGCAGAGCGA GGTATGTAGG 
     4921 CGGTGCTACA GAGTTCTTGA AGTGGTGGCC TAACTACGGC TACACTAGAA GAACAGTATT 
     4981 TGGTATCTGC GCTCTGCTGA AGCCAGTTAC CTTCGGAAAA AGAGTTGGTA GCTCTTGATC 
     5041 CGGCAAACAA ACCACCGCTG GTAGCGGTGG TTTTTTTGTT TGCAAGCAGC AGATTACGCG 
     5101 CAGAAAAAAA GGATCTCAAG AAGATCCTTT GATCTTTTCT ACGGGGTCTG ACGCTCAGTG 
     5161 GAACGAAAAC TCACGTTAAG GGATTTTGGT CATGAGATTA TCAAAAAGGA TCTTCACCTA 
     5221 GATCCTTTTA AATTAAAAAT GAAGTTTTAA ATCAATCTAA AGTATATATG AGTAACCTGA 
     5281 GGCTATGGCA GGGCCTGCCG CCCCGACGTT GGCTGCGAGC CCTGGGCCTT CACCCGAACT 
     5341 TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG 
     5401 TGGGGTATCG ACAGAGTGCC AGCCCTGGGA CCGAACCCCG CGTTTATGAA CAAACGACCC 
     5461 AACACCGTGC GTTTTATTCT GTCTTTTTAT TGCCGTCATA GCGCGGGTTC CTTCCGGTAT 
     5521 TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC CCTAGGGTGG GCGAAGAACT CCAGCATGAG 
     5581 ATCCCCGCGC TGGAGGATCA TCCAGCCGGC GTCCCGGAAA ACGATTCCGA AGCCCAACCT 
     5641 TTCATAGAAG GCGGCGGTGG AATCGAAATC TCGTGATGGC AGGTTGGGCG TCGCTTGGTC 
     5701 GGTCATTTCG AACCCCAGAG TCCCGCTCAG AAGAACTCGT CAAGAAGGCG ATAGAAGGCG 
     5761 ATGCGCTGCG AATCGGGAGC GGCGATACCG TAAAGCACGA GGAAGCGGTC AGCCCATTCG 
     5821 CCGCCAAGCT CTTCAGCAAT ATCACGGGTA GCCAACGCTA TGTCCTGATA GCGATCCGCC 
     5881 ACACCCAGCC GGCCACAGTC GATGAATCCA GAAAAGCGGC CATTTTCCAC CATGATATTC 
     5941 GGCAAGCAGG CATCGCCATG GGTCACGACG AGATCCTCGC CGTCGGGCAT GCTCGCCTTG 
     6001 AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC CCCTGATGCT CTTCGTCCAG ATCATCCTGA 
     6061 TCGACAAGAC CGGCTTCCAT CCGAGTACGT GCTCGCTCGA TGCGATGTTT CGCTTGGTGG 
     6121 TCGAATGGGC AGGTAGCCGG ATCAAGCGTA TGCAGCCGCC GCATTGCATC AGCCATGATG 
     6181 GATACTTTCT CGGCAGGAGC AAGGTGAGAT GACAGGAGAT CCTGCCCCGG CACTTCGCCC 
     6241 AATAGCAGCC AGTCCCTTCC CGCTTCAGTG ACAACGTCGA GCACAGCTGC GCAAGGAACG 
     6301 CCCGTCGTGG CCAGCCACGA TAGCCGCGCT GCCTCGTCTT GCAGTTCATT CAGGGCACCG 
     6361 GACAGGTCGG TCTTGACAAA AAGAACCGGG CGCCCCTGCG CTGACAGCCG GAACACGGCG 
     6421 GCATCAGAGC AGCCGATTGT CTGTTGTGCC CAGTCATAGC CGAATAGCCT CTCCACCCAA 
     6481 GCGGCCGGAG AACCTGCGTG CAATCCATCT TGTTCAATCA TGCGAAACGA TCCTCATCCT 
     6541 GTCTCTTGAT CGATCTTTGC AAAAGCCTAG GCCTCCAAAA AAGCCTCCTC ACTACTTCTG 
     6601 GAATAGCTCA GAGGCCGAGG CGGCCTCGGC CTCTGCATAA ATAAAAAAAA TTAGTCAGCC 
     6661 ATGGGGCGGA GAATGGGCGG AACTGGGCGG AGTTAGGGGC GGGATGGGCG GAGTTAGGGG 
     6721 CGGGACTATG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6781 GCCTGGGGAC TTTCCACACC TGGTTGCTGA CTAATTGAGA TGCATGCTTT GCATACTTCT 
     6841 GCCTGCTGGG GAGCCTGGGG ACTTTCCACA CCCTAACTGA CACACATTCC ACAGCTGGTT 
     6901 CTTTCCGCCT CAGGACTCTT CCTTTTTCAA TATTATTGAA GCATTTATCA GGGTTATTGT 
     6961 CTCATGAGCG GATACATATT TGAATGTATT TAGAAAAATA AACAAATAGG GGTTCCGCGC 
     7021 ACATTTCCCC GAAAAGTGCC ACCTGACGCG CCCTGTAGCG GCGCATTAAG CGCGGCGGGT 
     7081 GTGGTGGTTA CGCGCAGCGT GACCGCTACA CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC 
     7141 GCTTTCTTCC CTTCCTTTCT CGCCACGTTC GCCGGCTTTC CCCGTCAAGC TCTAAATCGG 
     7201 GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT TTACGGCACC TCGACCCCAA AAAACTTGAT 
     7261 TAGGGTGATG GTTCACGTAG TGGGCCATCG CCCTGATAGA CGGTTTTTCG CCCTTTGACG 
     7321 TTGGAGTCCA CGTTCTTTAA TAGTGGACTC TTGTTCCAAA CTGGAACAAC ACTCAACCCT 
     7381 ATCTCGGTCT ATTCTTTTGA TTTATAAGGG ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA 
     7441 AATGAGCTGA TTTAACAAAA ATTTAACGCG AATTTT
//