LOCUS       RR212259                8064 bp    DNA     circular  SYN 
DEFINITION  Pum2 (Myc-DDK-tagged ORF) - Rat pumilio homolog 2 (Drosophila) (Pum2), (10 ug)
ACCESSION   NM_001106715
VERSION     NM_001106715.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4221..4312 
                     /label="Myc-DDK"  
     misc_feature    4342..4927 
                     /label="PolyA signal"  
     misc_feature    4399..4418 
                     /label="Restriction Enzyme"  
     misc_feature    5203..5875 
                     /label="ColE1"  
     misc_feature    6305..7099 
                     /label="Kan/Neo"  
     misc_feature    7134..7436 
                     /label="SV40 ori"  
     misc_feature    7522..7999 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4203..4209 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4203 
                     /label="Pum2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAATCATGAT TTTCAAGCTC TTGCATTAGA ATCTCGGGGA ATGGGAGAGC 
     1081 TTTTGCCTAC CAAAAAATTT TGGGAACCTG ATGATTCAAC AAAAGATGGA CAAAAAGGCA 
     1141 TATTTCTTGG GGATGATGAA TGGAGAGAGA CTGCATGGGG AACTTCTCAT CATTCAATGT 
     1201 CCCAGCCTAT TATGGTACAG AGAAGATCTG GACAGAGTTT TCATGGAAAC AGTGAAGTAA 
     1261 ATGCAATTCT TTCTCCGCGC TCAGAAAGTG GAGGCCTTGG CGTGAGCATG GTAGAATATG 
     1321 TATTAAGTTC CTCTCCTGCT GATAAATTGG ATTCTCGATT TAGGAAGGGA ACTTTTGGTA 
     1381 CTAGAGATGC TGAAACAGAT GGACCTGAGA AAGGAGATCA AAAAGGCAAG GCTTCTCCAT 
     1441 TTGAGGAGGA CCAAAACAGA GATCTTAAAC AAGATGATGA GGACTCTAAA ATAAATGGCA 
     1501 GAGGTTTGCC AAATGGAATG GATGCCGATT GCAAAGATTT TAATCGTACT CCGGGAAGTC 
     1561 GTCAAGCCTC TCCAACTGAA GTAGTTGAGC GCCTTGGCCC TAGTACTAAT CCCCCAGAAG 
     1621 GATTGGGTCC TCTTCCTAAT CCGACAGCGA ATAAACCACT TGTTGAAGAA TTTTCAAATC 
     1681 CTGAAACTCA GAATCTGGAT GCAATGGACC AAGTTGGTCT GGATTCCTTA CAGTTTGACT 
     1741 ATCCTGGTAA TCAGGTACCC ATGGATTCTT CAGGAGCTAC TGTAGGCCTT TTTGACTACA 
     1801 ATTCCCAACA GCAGCTCTTT CAGAGGACTA ATGCACTAAC AGTTCAGCAG TTAACTGCTG 
     1861 CTCAACAGCA GCAGTATGCA TTAGCAGCAG CTCAGCAGCC GCATATAGCT GGTGTATTCT 
     1921 CAGCAGGCCT TGCTCCAGCT GCATTTGTGC CAAATCCATA TATTATTAGT GCTGCTCCTC 
     1981 CAGGGACCGA CCCGTATACT GCAGCAGGAT TGGCTGCAGC AGCAACATTA GCAGGTCCAG 
     2041 CAGTGGTTCC ACCTCAGTAT TATGGTGTTC CATGGGGGGT GTATCCAGCC AATTTATTTC 
     2101 AGCAACAAGC TGCAGCTGCG GCAAGCAACA CAGCCAATCA GCAAGCAGCA TCACAAGCTC 
     2161 AGCCTGGACA GCAGCAGGTT CTTCGTCCTG GAGCAGGTCA ACGTCCTATT ACTCCCAGTC 
     2221 AGGGCCAACA AGGGCAGCAA GCAGAGTCAC TTGCAGCAGC CGCAGCTGCA AACCCAACTT 
     2281 TGGCTTTTGG TCAGAGTCTT GCTGCAGGCA TGCCAGGCTA TCAAGTACTA GCTCCAACTG 
     2341 CCTATTATGA TCAGACTGGT GCCTTAGTGG TTGGCCCTGG AGCAAGAACT GGCCTTGGAG 
     2401 CTCCAGTACG ATTAATGGCT CCAACACCTG TCTTAATTAG TTCAGCAGCA GCACAAGCTG 
     2461 CAGCAGCAGC AGCAGCAGCT GGAGGAACTG CAAATAGTCT TACAGGCAGC ACAAACGGTC 
     2521 TGTTTCGGCC GATTGGCACT CAGCCACCGC AGCAGCAGCA GCAGCAGCAG CCAAGCACTA 
     2581 ACCTGCAGTC TAATTCGTTT TATGGGAGCA GCTCTTTGAC TAACAGCTCC CAGAGCAGCT 
     2641 CTTTATTTTC TCACGGACCT GGCCAACCTG GAAGTGCATC TCTTGGCTTT GGAAGTGGTA 
     2701 GCTCTTTGGG AGCCGCTATA GGCTCAGCTC TCAGTGGATT TGGCTCATCA GTTGGCAGTT 
     2761 CTGCAAGTAG TAGTGCCACA AGGAGAGAGT CTCTATCTAC TAGCTCTGAC TTGTACAAAA 
     2821 GATCTAGTAG CAGCCTAGCA CCCATAGGGC AACCATTTTA CAATAGTCTG GGATTTTCCT 
     2881 CCTCTCCAAG TCCAATAGGC ATGCCTCTGC CAAGCCAAAC TCCAGGACAT TCACTTACGC 
     2941 CACCGCCATC ACTTTCATCA CATGGATCCT CATCCAGAGG CCTGACAAAT GGTAGTGGTC 
     3001 GGTACATCTC TGCAGCACCT GGAGCGGAAG CAAAGTACCG GAGCGCTTCC AGCACTTCCA 
     3061 GCCTGTTCAG CTCCAGCAGC CAGCTCTTTC CTCCCTCTCG GCTCCGCTAT AACAGATCTG 
     3121 ATATCATGCC CTCTGGCCGC AGCAGGCTAT TGGAAGATTT CAGGAACAAC CGCTTCCCCA 
     3181 ACCTCCAGCT TAGAGACTTG ATGGGACATA TAGTCGAGTT TTCTCAAGAC CAGCATGGCT 
     3241 CTAGATTCAT ACAGCAGAAG CTAGAGAGAG CTACTCCAGC TGAGCGACAG ATGGTATTTA 
     3301 ATGAAATTGT ACAGGCTGCC TATCAATTAA TGACAGATGT TTTTGGAAAC TATGTTATAC 
     3361 AGAAGTTTTT TGAGTTTGGA AGTTTGGATC AGAAATTAGC CCTGGCTAAT CGTATTCGTG 
     3421 GTCATGTTCT ACCATTAGCC TTGCAGATGT ATGGCTGCCG TGTTATTCAA AAAGCATTAG 
     3481 AATCTATTTC TTCTGATCAG CAGAGTGAAA TGGTCAAGGA ACTTGATGGT CATGTACTTA 
     3541 AATGTGTGAA AGATCAGAAT GGAAACCATG TTGTACAGAA GTGTATTGAA TGTGTTCAAC 
     3601 CGCAGTCACT GCAGTTCATC ATCGATGCTT TCAAAGGACA AGTATTTGTG CTTTCAACCC 
     3661 ATCCTTACGG CTGCAGAGTC ATTCAGCGGA TCTTAGAGCA CTGTACTGCA GAGCAGACTT 
     3721 TACCCATCTT AGAAGAGCTT CACCAACACA CAGAGCAGTT GGTACAGGAT CAGTATGGCA 
     3781 ATTATGTTAT TCAGCATGTA CTGGAACATG GTCGGCCTGA AGACAAGAGC AAAATTGTTT 
     3841 CTGAAATCAG AGGAAAGGTC TTAGCCCTGA GTCAACACAA ATTTGCCAGC AATGTAGTAG 
     3901 AAAAGTGTGT TACTCATGCC TCACGTGCTG AGAGAGCTTT ACTGATTGAT GAGGTCTGCT 
     3961 GTCAGAATGA TGGTCCTCAC AGTGCCTTAT ACACCATGAT GAAGGATCAA TATGCCAACT 
     4021 ATGTGGTTCA GAAGATGATT GATATGGCTG AGCCTGCTCA GAGAAAGATA ATCATGCACA 
     4081 AGATTCGACC TCACATCACT ACTCTTCGCA AATACACATA TGGGAAGCAT ATACTGGCCA 
     4141 AGTTGGAAAA ATACTATTTG AAAAACAGCC CAGATCTAGG GCCTATTGGA GGACCACCAA 
     4201 ATGGAATGCT GACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     4261 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     4321 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     4381 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     4441 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     4501 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     4561 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     4621 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     4681 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     4741 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     4801 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     4861 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     4921 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     4981 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     5041 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     5101 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     5161 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     5221 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     5281 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     5341 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     5401 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     5461 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     5521 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     5581 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     5641 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     5701 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     5761 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     5821 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     5881 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     5941 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     6001 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     6061 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     6121 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     6181 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     6241 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     6301 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     6361 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     6421 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     6481 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     6541 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     6601 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     6661 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     6721 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     6781 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     6841 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     6901 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     6961 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     7021 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     7081 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     7141 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     7201 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     7261 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     7321 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     7381 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     7441 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     7501 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     7561 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     7621 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     7681 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     7741 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     7801 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     7861 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     7921 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     7981 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     8041 TAACAAAAAT TTAACGCGAA TTTT
//