LOCUS       RR212226                8274 bp    DNA     circular  SYN 
DEFINITION  Rfc1 (Myc-DDK-tagged ORF) - Rat replication factor C (activator 1) 1 (Rfc1), (10 ug)
ACCESSION   NM_053547
VERSION     NM_053547.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4431..4522 
                     /label="Myc-DDK"  
     misc_feature    4552..5137 
                     /label="PolyA signal"  
     misc_feature    4609..4628 
                     /label="Restriction Enzyme"  
     misc_feature    5413..6085 
                     /label="ColE1"  
     misc_feature    6515..7309 
                     /label="Kan/Neo"  
     misc_feature    7344..7646 
                     /label="SV40 ori"  
     misc_feature    7732..8209 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4413..4419 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4413 
                     /label="Rfc1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGACATTCGG AAATTCTTTG GGGTTATATC AAGTGGAAAA AAGCCTGTAA 
     1081 ATGAGACAGT AAAGAAGAAT GAGAAGACAA AACCTTCGGA GGGCACTGTC AAAGGAAAGC 
     1141 AAGGAGTAAA GGAAGCCAAG GTTAATAATC CCTGCAAAGA GGATGCCTCC AGACCAAAGC 
     1201 AGCACAACAA GAAAAAGAGG ATCATCTATG ACTCAGATTC AGAATCAGAG GAGACAGTGC 
     1261 AAGTAAAAAA TGCTAAAAAG AAATCAGAAA AATTGCCAGT GTCTTGTAAA CCTGGTAAAA 
     1321 TCTCTCGGAA GGATCCTGTT ACCTATATTT CTGAGACAGA TGAAGACGAT GACTTTTTAT 
     1381 GTAAAAAGGC AGCCTCCAAA TCAAAAGAGA ATGGAGTATC TACAAACAGT TACCTTGGAG 
     1441 CATCAAACGT GAAAAAGAAT GAAGAAAACA CTAAGACTAA GAGTAAACCG TTATCACCAA 
     1501 TAAAACTAAC ACCGACATCA GTGCTTGATT ATTTTGGAAC TGAAAGTGTC CAGAGATCTG 
     1561 GGAAGAAGAT GGTGGCGAGC AAAAAGAAAG AGTCTTCTCA AACCCCAGAT GATTCCAGAT 
     1621 TAAATGATGA GGCCATCGCC AAGCAGCTTC AGCTTGATGA AGATGCAGAG CTGGAGAGGC 
     1681 AGTTGCATGA AGATGAAGAA TTTGCAAGAA CACTGGCCTT GTTGGATGAA GAACCTAAGA 
     1741 CCAAAAAGGC TCGAAAGGAC TCTGAAGAGG GAGAATCATT TTCACCTGCC AAAGCTGAGT 
     1801 TAAGCAAAGC AGCAAAGCAG AAGAGCCCTG CTAACGAGCA TTTCTCAATT GGAAGAAAGA 
     1861 CCTACAGTCC TGCTAAGTAC GGCAAGGGTA GAGGCTCAGA AGGCACCAAG CAGCCCTGCA 
     1921 GATCAGCTCA CCAGAAGGAA GCCTGCTCCT CTCTCAAGGC CAGCTCCAAG TCGGCTCTTA 
     1981 TGAAAGCACA AGAAGAAAAT TCTTACAAAG AAACAGAACT GCTGGCTGCA AAAAGAGAAA 
     2041 GTGCCATTGA GCCCAAAGGA GAGAAAACAA CTCCTAGGAA AACGAAAGGC TCTCCAACTA 
     2101 AAAGAGAGTC TGTAAGCCCA GAAGATTCTG AAAAGAAGCG CACCAATTAT CAAGCTTATC 
     2161 GAAGCTACTT AAATCGAGAA GGTCCCAAAG CCCTGGGCTC CAAAGAAATA CCCAAGGGAG 
     2221 CTGAAAACTG CTTGGAGGGC CTGACGTTCG TGATCACCGG AGTGCTGGAG TCCATCGAAC 
     2281 GAGAAGAAGC CAAGTCTCTA ATTGAACGTT ATGGGGGGAA AGTAACAGGA AACGTGAGCA 
     2341 AGAAAACCAG CTACCTCGTC ATGGGCCGGG ACAGCGGGCA GTCCAAGAGT GACAAGGCAG 
     2401 CAGCTCTGGG AACAAAAATC CTTGATGAAG ACGGCCTGTT GGATCTGATT CGAACTATGC 
     2461 CGGGCAAAAA ATCCAAGTAC GAAATCGCTG CTGAGGCTGA GATGAAGAAA GAAAAGTCCA 
     2521 AATTGGAGAG AACACCCCAA AAAAATGATC AGGGAAAAAG AAAACTTAGT CCAACCAAGA 
     2581 GGGAGTTGGA GCCTAAAAAG AGCAAGCTGA CTCCCCTTAA GCACAGCCCT CGGAAGGCAG 
     2641 TGAAGGAGGA GGAACGTGTG TGTCCGAGGG GCTTGGACGC CAAGGAGCCT CATGGCAGCC 
     2701 ACAGTGCCAG CAGAGAGGAG TGCCTGCTCT GGGTGGATAA GTACAAGCCC ACCTCACTCA 
     2761 AGAACATAAT CGGACAGCAG GGTGACCAGA GCTGTGCCAA CAAACTGCTA CGCTGGCTCA 
     2821 GAAACTGGCA CAAGAGTTCT CCAGAAGAGA AAAAGCATGC AGCAAAATTT GGCAAAGTTG 
     2881 CCAGCAAAGA TGATGGCTCC AGTTTCAAGG CAGCACTGCT GTCTGGCCCT CCAGGTGTTG 
     2941 GCAAAACCAC TACAGCTTCT CTTGTGTGTC AGGAATTGGG CTACAGTTAT GTGGAACTAA 
     3001 ATGCGAGTGA TACTCGGAGT AAGAACAGTC TGAAAGCAAT TGTTGCTGAG TCATTGAACA 
     3061 ACACCAGCAT CAAAGGCTTT TATACAAGTG GAGCATCTCC TTCTGTAAGT GCGAGGCATG 
     3121 CTCTCATCAT GGATGAAGTT GATGGCATGG CGGGCAATGA GGACAGGGGT GGAATTCAGG 
     3181 AGCTAATAGG CCTGATAAAG CATACGAAAA TTCCCATCAT TTGTATGTGC AATGATAGAA 
     3241 ATCACCCCAA AATCCGCTCA TTGGTTCATT ATTGTTTTGA TCTTCGTTTC CAAAGACCTC 
     3301 GAGTTGAACA GATTAAGAGT GCAATGCTGT CTATTGCATT TAAAGAGGGA TTAAAGATCC 
     3361 CACCTCCAGC TATGAATGAA ATAATTTTGG GAGCTAATCA AGATGTCAGA CAGGTTTTAC 
     3421 ACAATCTCAG TATGTGGTGT GCACAGAGTA AGGTCCTAAC CTATGACCAA GCCAAGGCTG 
     3481 ATTCTCAGAG GGCCAAGAAG GACATCAGAT TGGGCCCATT TGATGTCACC CGGAAAGTGT 
     3541 TTGCAGCTGG AGAGGAGACT GCACACATGT CACTGATGGA CAAATCGGAT CTCTTTTTCC 
     3601 ATGACTATTC CATTGCACCC CTCTTTGTCC AGGAGAACTA CCTCCATGTA AAGCCTGTGG 
     3661 CTGCAGGGGG TGACATGAAA AAGCACTTGA TGCTTTTAAG CCGGGCAGCA GATAGCATAT 
     3721 GTGACGGTGA CTTAGTGGAC AATCAGATCC GGAGTAAGCA AAACTGGAGT CTTCTGCCCA 
     3781 CACAGGCCAT TTACGCCAGC GTTGTTCCTG GAGAGTTGAT GAGGGGCTAC ATGTCTCAGT 
     3841 TTCCCAGCTT CCCAAGCTGG CTGGGAAAGC ACTCGTCTAT GGGCAAGCAT GATCGGACTG 
     3901 TTCAGGACCT GTCCTTGCAC ATGAGTCTTA GAACTTACTC CAGCAAAAGG ACAGTGAACA 
     3961 TGGATTACCT GTCACACATC AGAGACGCAC TTGTACGACC CTTGACCTCA CAAGGAGTAG 
     4021 AAGGGGTTCA GCGTGTTGTC ACACTTATGG ATACATACTA TTTGGTGAAA GAAGATTTTG 
     4081 ACAACATCAT GGAAGTTAGC AGCTGGGGGG GCAAGCCCAG TACCTTTTCC AAGCTGGACC 
     4141 CCAAGGTGAA AGCAGCCTTC ACAAGAGCCT ATAATAAGGA GGCCCACCTG ACCCCATACT 
     4201 CACTTCAGGT GGTAAAGGCA TCGAGGCCCA GCATAGGTCC AGCACTGGAT TCTGAGTACA 
     4261 ATGAAGAGTC ACAGGAAGAT GCCACTCAGT CTGATGAGAA AGAGCAGGAT GCCCTAGAGA 
     4321 CTGATGCCAT GATCAAGCAG AAAAAGACGA GATCTTCAAA GCCTTCAAAA TCAGAAAAAG 
     4381 AGAAGGAGTC CAAAAAAGGA AAAGGAAAAA ATTCGAAGAA AACGCGTACG CGGCCGCTCG 
     4441 AGCAGAAACT CATCTCAGAA GAGGATCTGG CAGCAAATGA TATCCTGGAT TACAAGGATG 
     4501 ACGACGATAA GGTTTAAACG GCCGGCCGCG GTCATAGCTG TTTCCTGAAC AGATCCCGGG 
     4561 TGGCATCCCT GTGACCCCTC CCCAGTGCCT CTCCTGGCCC TGGAAGTTGC CACTCCAGTG 
     4621 CCCACCAGCC TTGTCCTAAT AAAATTAAGT TGCATCATTT TGTCTGACTA GGTGTCCTTC 
     4681 TATAATATTA TGGGGTGGAG GGGGGTGGTA TGGAGCAAGG GGCAAGTTGG GAAGACAACC 
     4741 TGTAGGGCCT GCGGGGTCTA TTGGGAACCA AGCTGGAGTG CAGTGGCACA ATCTTGGCTC 
     4801 ACTGCAATCT CCGCCTCCTG GGTTCAAGCG ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG 
     4861 GGATTCCAGG CATGCATGAC CAGGCTCAGC TAATTTTTGT TTTTTTGGTA GAGACGGGGT 
     4921 TTCACCATAT TGGCCAGGCT GGTCTCCAAC TCCTAATCTC AGGTGATCTA CCCACCTTGG 
     4981 CCTCCCAAAT TGCTGGGATT ACAGGCGTGA ACCACTGCTC CCTTCCCTGT CCTTCTGATT 
     5041 TTAAAATAAC TATACCAGCA GGAGGACGTC CAGACACAGC ATAGGCTACC TGGCCATGCC 
     5101 CAACCGGTGG GACATTTGAG TTGCTTGCTT GGCACTGTCC TCTCATGCGT TGGGTCCACT 
     5161 CAGTAGATGC CTGTTGAATT GGGTACGCGG CCAGCGGCGA GCGGTATCAG CTCACTCAAA 
     5221 GGCGGTAATA CGGTTATCCA CAGAATCAGG GGATAACGCA GGAAAGAACA TGTGAGCAAA 
     5281 AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA GGCCGCGTTG CTGGCGTTTT TCCATAGGCT 
     5341 CCGCCCCCCT GACGAGCATC ACAAAAATCG ACGCTCAAGT CAGAGGTGGC GAAACCCGAC 
     5401 AGGACTATAA AGATACCAGG CGTTTCCCCC TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC 
     5461 GACCCTGCCG CTTACCGGAT ACCTGTCCGC CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC 
     5521 TCATAGCTCA CGCTGTAGGT ATCTCAGTTC GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG 
     5581 TGTGCACGAA CCCCCCGTTC AGCCCGACCG CTGCGCCTTA TCCGGTAACT ATCGTCTTGA 
     5641 GTCCAACCCG GTAAGACACG ACTTATCGCC ACTGGCAGCA GCCACTGGTA ACAGGATTAG 
     5701 CAGAGCGAGG TATGTAGGCG GTGCTACAGA GTTCTTGAAG TGGTGGCCTA ACTACGGCTA 
     5761 CACTAGAAGA ACAGTATTTG GTATCTGCGC TCTGCTGAAG CCAGTTACCT TCGGAAAAAG 
     5821 AGTTGGTAGC TCTTGATCCG GCAAACAAAC CACCGCTGGT AGCGGTGGTT TTTTTGTTTG 
     5881 CAAGCAGCAG ATTACGCGCA GAAAAAAAGG ATCTCAAGAA GATCCTTTGA TCTTTTCTAC 
     5941 GGGGTCTGAC GCTCAGTGGA ACGAAAACTC ACGTTAAGGG ATTTTGGTCA TGAGATTATC 
     6001 AAAAAGGATC TTCACCTAGA TCCTTTTAAA TTAAAAATGA AGTTTTAAAT CAATCTAAAG 
     6061 TATATATGAG TAACCTGAGG CTATGGCAGG GCCTGCCGCC CCGACGTTGG CTGCGAGCCC 
     6121 TGGGCCTTCA CCCGAACTTG GGGGGTGGGG TGGGGAAAAG GAAGAAACGC GGGCGTATTG 
     6181 GCCCCAATGG GGTCTCGGTG GGGTATCGAC AGAGTGCCAG CCCTGGGACC GAACCCCGCG 
     6241 TTTATGAACA AACGACCCAA CACCGTGCGT TTTATTCTGT CTTTTTATTG CCGTCATAGC 
     6301 GCGGGTTCCT TCCGGTATTG TCTCCTTCCG TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC 
     6361 GAAGAACTCC AGCATGAGAT CCCCGCGCTG GAGGATCATC CAGCCGGCGT CCCGGAAAAC 
     6421 GATTCCGAAG CCCAACCTTT CATAGAAGGC GGCGGTGGAA TCGAAATCTC GTGATGGCAG 
     6481 GTTGGGCGTC GCTTGGTCGG TCATTTCGAA CCCCAGAGTC CCGCTCAGAA GAACTCGTCA 
     6541 AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA TCGGGAGCGG CGATACCGTA AAGCACGAGG 
     6601 AAGCGGTCAG CCCATTCGCC GCCAAGCTCT TCAGCAATAT CACGGGTAGC CAACGCTATG 
     6661 TCCTGATAGC GATCCGCCAC ACCCAGCCGG CCACAGTCGA TGAATCCAGA AAAGCGGCCA 
     6721 TTTTCCACCA TGATATTCGG CAAGCAGGCA TCGCCATGGG TCACGACGAG ATCCTCGCCG 
     6781 TCGGGCATGC TCGCCTTGAG CCTGGCGAAC AGTTCGGCTG GCGCGAGCCC CTGATGCTCT 
     6841 TCGTCCAGAT CATCCTGATC GACAAGACCG GCTTCCATCC GAGTACGTGC TCGCTCGATG 
     6901 CGATGTTTCG CTTGGTGGTC GAATGGGCAG GTAGCCGGAT CAAGCGTATG CAGCCGCCGC 
     6961 ATTGCATCAG CCATGATGGA TACTTTCTCG GCAGGAGCAA GGTGAGATGA CAGGAGATCC 
     7021 TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG TCCCTTCCCG CTTCAGTGAC AACGTCGAGC 
     7081 ACAGCTGCGC AAGGAACGCC CGTCGTGGCC AGCCACGATA GCCGCGCTGC CTCGTCTTGC 
     7141 AGTTCATTCA GGGCACCGGA CAGGTCGGTC TTGACAAAAA GAACCGGGCG CCCCTGCGCT 
     7201 GACAGCCGGA ACACGGCGGC ATCAGAGCAG CCGATTGTCT GTTGTGCCCA GTCATAGCCG 
     7261 AATAGCCTCT CCACCCAAGC GGCCGGAGAA CCTGCGTGCA ATCCATCTTG TTCAATCATG 
     7321 CGAAACGATC CTCATCCTGT CTCTTGATCG ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA 
     7381 GCCTCCTCAC TACTTCTGGA ATAGCTCAGA GGCCGAGGCG GCCTCGGCCT CTGCATAAAT 
     7441 AAAAAAAATT AGTCAGCCAT GGGGCGGAGA ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG 
     7501 GATGGGCGGA GTTAGGGGCG GGACTATGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7561 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCTG GTTGCTGACT AATTGAGATG 
     7621 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC CTAACTGACA 
     7681 CACATTCCAC AGCTGGTTCT TTCCGCCTCA GGACTCTTCC TTTTTCAATA TTATTGAAGC 
     7741 ATTTATCAGG GTTATTGTCT CATGAGCGGA TACATATTTG AATGTATTTA GAAAAATAAA 
     7801 CAAATAGGGG TTCCGCGCAC ATTTCCCCGA AAAGTGCCAC CTGACGCGCC CTGTAGCGGC 
     7861 GCATTAAGCG CGGCGGGTGT GGTGGTTACG CGCAGCGTGA CCGCTACACT TGCCAGCGCC 
     7921 CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT TCCTTTCTCG CCACGTTCGC CGGCTTTCCC 
     7981 CGTCAAGCTC TAAATCGGGG GCTCCCTTTA GGGTTCCGAT TTAGTGCTTT ACGGCACCTC 
     8041 GACCCCAAAA AACTTGATTA GGGTGATGGT TCACGTAGTG GGCCATCGCC CTGATAGACG 
     8101 GTTTTTCGCC CTTTGACGTT GGAGTCCACG TTCTTTAATA GTGGACTCTT GTTCCAAACT 
     8161 GGAACAACAC TCAACCCTAT CTCGGTCTAT TCTTTTGATT TATAAGGGAT TTTGCCGATT 
     8221 TCGGCCTATT GGTTAAAAAA TGAGCTGATT TAACAAAAAT TTAACGCGAA TTTT
//