LOCUS       RR212205                8322 bp    DNA     circular  SYN 
DEFINITION  Prg4 (Myc-DDK-tagged ORF) - Rat proteoglycan 4, (megakaryocyte stimulating factor, articular superficial zone protein, camptodactyly, arthropathy, coxa vara, pericarditis syndrome) (Prg4, (10 ug)
ACCESSION   NM_001105962
VERSION     NM_001105962.3
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4479..4570 
                     /label="Myc-DDK"  
     misc_feature    4600..5185 
                     /label="PolyA signal"  
     misc_feature    4657..4676 
                     /label="Restriction Enzyme"  
     misc_feature    5461..6133 
                     /label="ColE1"  
     misc_feature    6563..7357 
                     /label="Kan/Neo"  
     misc_feature    7392..7694 
                     /label="SV40 ori"  
     misc_feature    7780..8257 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4461..4467 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4461 
                     /label="Prg4"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGTGGAAA ATACTTCCCA TCTGCTTGTC GATGCTGCTT CCTGTTTTCC 
     1081 TGATTCAACA AGTTTCATCT CAAGATATAT CAAGTTGTGC AGGGAGATGT GGGGAAGGGT 
     1141 ATTCTAGAGA CGCCACCTGC AACTGTGATT ATAACTGCCA ACACTACATG GAGTGCTGTC 
     1201 CTGATTTCAA GAGAGTCTGC ACTACAGAGC TCTCCTGCAA AGGACGCTGC TTCGAGTCCT 
     1261 TCGCACGAGG GAGGGAGTGT GACTGTGACT CCCAGTGTAA ACAGTACGGC AAATGCTGTG 
     1321 CAGACTACAA CAGCTTCTGC GAAGAAGTGC ATAATCCCAC ATCACCACCT CCAAAGACTG 
     1381 CACCCCCGCC TCCAGGAGCG TCTCGCACCA TCAAATCAAC CACCAAACGT TCACCCAAAT 
     1441 CACCAAAAAA GAAGACTAAG AAAGTTGTAG AGTCTGAGGA AATAACAGAA GAACATTCTG 
     1501 TTTCTGAAAA TCAAGAGTCC TCCTCCTCCT CCTCTTCCTC CTCTTCAACT ATTCGGAAAA 
     1561 TCAAGTCTTC CAAAAATTCA GCTAATAGAG AATTACAGAA GAACCCAAAA GTAAAAGATA 
     1621 ACAAGAAAAA CACCCCGAAA AAGAAACCTA ATCCAGAACC ACCAGTCGTG GATGAAGCTG 
     1681 GAAGTGGTCT GGACAATGGT GAGCCCAAGC TTACCCCACC TCCTGACCCT CCTACTCCAC 
     1741 ACAACAAAGT CACCGCATCT CCCAAGACTA CAGCGGCAAA ACCAGTAACT CCTAAACCCA 
     1801 GTCTTGCACC TAATTCTGAG ACTTCCAAAG AGGCATCTTC AACTTCCAAT AAAGAGACAA 
     1861 CAGTTGAAAC AAAGGAGACC ACTGAAACAA ATAAACAGTC TTCCGCCAGT AAAAAAGAGA 
     1921 AGACTACTTC AGCTAAAGAG ACACGGAGTG CAGAGAACAC ATCTGCTAAA GACGTTGAAC 
     1981 CCACATCTAC AACTCCCAAG AACTCAGCTC CCACTACCAC CAAGAAGCCT GTAACTACAA 
     2041 CCAAGGAACC TGTTCCTACC ACTACCAAGG GGCCTGAACC CACCTCCAAG GAGCCAGCTC 
     2101 CCACCACCCC TAAGGAGCCT GAACTCACCA TTCCCAAAGA ACCAGCTCCC ACCACCAAGA 
     2161 AGCCAGCTCC CACCACCCCC AAGGAGCCAG CTCCCACCAC CCCTAAGGAG CCAGCTCCCA 
     2221 CCACCCCCAA GGAGCCAGCT CCCACCACCA AGAAGCCTGA ACCCACCACA CCTAAGGAGC 
     2281 CAGCTCCCAC CACCCCCAAG GAGCCAGCTC CCACCACCCC CAAGGAGCCA GCTCCCAATA 
     2341 CCCCTAAGGA GCCAGCTCCC ACCACCCCCA AGGAGCCAGC TCCCACCACC CCTAAGGAGC 
     2401 CTGAATCCAC CACCCCCAAG GAACCAGCTC CCACCACCCC CAAGGAGCCA GCTCCCACCA 
     2461 CCCCCAAGGA GCCAGCTCCC ACCACCCCCA AGGAGCCTGC TCCCACTACC CCTAAGGAGC 
     2521 CAGCTCCCAC CACCCCCAAG GAGCCAGCTC TTACTACCCC TAAGGAGCCA GCTCCCACCC 
     2581 CCAAAGAGCC TGAACCCACC ACCCCTAAGG AGCCAGCTCC CACCACCCCT AAGGAGCCAG 
     2641 CTCCCACCAC CAAGAAGCCT GAACTCACCA TCCCCAAGGA GCCAGTTCCT ACCACCCCCA 
     2701 AAGAACCAGC TCCTACCACT CCCAAAGAGC CTGCACCCAC CACCACCAAG AAGCCTGAAC 
     2761 TCAGCACCCC TAAGGAGCCC ACATCTACTA ACCCCAAGGA ACCTGAACCC ACCCTCCCCA 
     2821 AGGAGCCTGA ACTCACCCTC CCCGAGGAGC CTGAACCCAG CAACCCCGAG ACATCCGATG 
     2881 AATTAATAGC TGAGCTTGCT GAGGCACCCA CACCAAAGGC TCTTGAAAAT AATCCCAAGG 
     2941 AACCCACTGT ACCAACTGAG ACTCCTGAGG TAAGCAAGCC TGAAATGACA ACTGCTAAAG 
     3001 AGAAGACAAC AGAAAAAGAC ACAACCCAAA CTACAACAAC AGTAACCCCT AAGACAACAA 
     3061 CAGAGACAAC AATCATACCG GAAGAGACTA CTGAACCAAG AGCAAGTACC ACCACTCGAA 
     3121 TAACGTCTAC AGCTCAAGAT ACCACATCAC CCCAAATGAC TACTCTGAAA GCTACAACTC 
     3181 TTGCAGCCAA AGTAACTGCT CCAACAGAAG AGATTCAGAG CAAACCAGAA GAAACAACTT 
     3241 TAACAACTCC TGCATTGGAA GGTTCTGATG CTTCTAAGAC AACCCTAAAA CCTCAGAAAC 
     3301 CAACCAAAGC GCCCAAGAAG CCCGCCTCTA CCAAAAAGCC AACCAAAGCG CCCAAGAAGC 
     3361 CCACCTCTAC CAAAAAGCCA AAGACACCAA AAGGGAGAAA ACCAAAAACC ACACCATCTC 
     3421 CTCTTAAGAC TTCAACAATG TCTGAACAGA ATACCACTCC TCTAGAAGTC ATGCTGCCAA 
     3481 CCACCACCGT CCCTAAACAA ACTCCAAACT CTGAAACAGC TGAAGTGAAT CCAGCTCATG 
     3541 AAGATGCAGA TGGAGGTGAA GGAGAAAAAC CTCTGATTCC CAGGCCCCCT GTGCTATCCC 
     3601 CCACTGCTGT TCCAGGAACC GATCTCTTGG TTGAGAGACT CAATCCAGGC ATTAACATCC 
     3661 ATCCCATGTT TTCAGATGAG ACCAATATAT GCAATGGTAA GCCAGTGGAT GGACTGACCA 
     3721 CTCTGCGCAA CGGGACGTTA GTTGCATTTC GAGGTCATTA TTTCTGGATG CTGAACCCAT 
     3781 TCAGACCACC ATCTCCACCA CGCAGAATCA CTGAAGTCTG GGGTATTCCC TCTCCCATTG 
     3841 ACACGGTTTT TACTAGATGT AACTGTGAAG GAAAAACTTT CTTCTTTAAG GATTCTCAGT 
     3901 ACTGGCGCTT TACCAACGAT GTAATGGATG CTGGGTATCC TAAACTAATT GTCAAAGGCT 
     3961 TTGGAGGACT AACAGGGAAG ATAGTGGCTG CTCTTTCAAT AGCCAAGTAC AAGGACAGAC 
     4021 CTGAATCTGT GTACTTCTTC AAGAGAGGTG GCAACATCCA GCAGTACACT TACAAACAGG 
     4081 AGCCCGTGAG GAAGTGCACA GGGAGGCAGC CTGCCATCAA CTACTCAGTG TATGGGCAGG 
     4141 CAGCACAGGT CAGGAGGCGC CGCTTTGAGC GTGCTGTTGG ACCTTTCCAG ACACACACCT 
     4201 TCAGGATCCA TTACTCGGTG CCCATCAAAG CCTCTTATCA AGACAAAGGC TTCCTCCACA 
     4261 ATGAAGTCAA AGTGAGCACA CTGTGGAGAG GGTTCCCAAA TGTCGTTACT TCCGCGATAA 
     4321 CACTGCCCAA CATTAGAAAA CCTGACGGCT ATGATTACTA CGCCTTTTCC AAAGATCAAT 
     4381 ACTATAACAT CGATGTGCCT ACTAGAACAG CAAGAGCAAT CACCACGCGC TCAGGGCAGA 
     4441 CCCTCTCCAG AGTCTGGTAC AACTGTCCTA CGCGTACGCG GCCGCTCGAG CAGAAACTCA 
     4501 TCTCAGAAGA GGATCTGGCA GCAAATGATA TCCTGGATTA CAAGGATGAC GACGATAAGG 
     4561 TTTAAACGGC CGGCCGCGGT CATAGCTGTT TCCTGAACAG ATCCCGGGTG GCATCCCTGT 
     4621 GACCCCTCCC CAGTGCCTCT CCTGGCCCTG GAAGTTGCCA CTCCAGTGCC CACCAGCCTT 
     4681 GTCCTAATAA AATTAAGTTG CATCATTTTG TCTGACTAGG TGTCCTTCTA TAATATTATG 
     4741 GGGTGGAGGG GGGTGGTATG GAGCAAGGGG CAAGTTGGGA AGACAACCTG TAGGGCCTGC 
     4801 GGGGTCTATT GGGAACCAAG CTGGAGTGCA GTGGCACAAT CTTGGCTCAC TGCAATCTCC 
     4861 GCCTCCTGGG TTCAAGCGAT TCTCCTGCCT CAGCCTCCCG AGTTGTTGGG ATTCCAGGCA 
     4921 TGCATGACCA GGCTCAGCTA ATTTTTGTTT TTTTGGTAGA GACGGGGTTT CACCATATTG 
     4981 GCCAGGCTGG TCTCCAACTC CTAATCTCAG GTGATCTACC CACCTTGGCC TCCCAAATTG 
     5041 CTGGGATTAC AGGCGTGAAC CACTGCTCCC TTCCCTGTCC TTCTGATTTT AAAATAACTA 
     5101 TACCAGCAGG AGGACGTCCA GACACAGCAT AGGCTACCTG GCCATGCCCA ACCGGTGGGA 
     5161 CATTTGAGTT GCTTGCTTGG CACTGTCCTC TCATGCGTTG GGTCCACTCA GTAGATGCCT 
     5221 GTTGAATTGG GTACGCGGCC AGCGGCGAGC GGTATCAGCT CACTCAAAGG CGGTAATACG 
     5281 GTTATCCACA GAATCAGGGG ATAACGCAGG AAAGAACATG TGAGCAAAAG GCCAGCAAAA 
     5341 GGCCAGGAAC CGTAAAAAGG CCGCGTTGCT GGCGTTTTTC CATAGGCTCC GCCCCCCTGA 
     5401 CGAGCATCAC AAAAATCGAC GCTCAAGTCA GAGGTGGCGA AACCCGACAG GACTATAAAG 
     5461 ATACCAGGCG TTTCCCCCTG GAAGCTCCCT CGTGCGCTCT CCTGTTCCGA CCCTGCCGCT 
     5521 TACCGGATAC CTGTCCGCCT TTCTCCCTTC GGGAAGCGTG GCGCTTTCTC ATAGCTCACG 
     5581 CTGTAGGTAT CTCAGTTCGG TGTAGGTCGT TCGCTCCAAG CTGGGCTGTG TGCACGAACC 
     5641 CCCCGTTCAG CCCGACCGCT GCGCCTTATC CGGTAACTAT CGTCTTGAGT CCAACCCGGT 
     5701 AAGACACGAC TTATCGCCAC TGGCAGCAGC CACTGGTAAC AGGATTAGCA GAGCGAGGTA 
     5761 TGTAGGCGGT GCTACAGAGT TCTTGAAGTG GTGGCCTAAC TACGGCTACA CTAGAAGAAC 
     5821 AGTATTTGGT ATCTGCGCTC TGCTGAAGCC AGTTACCTTC GGAAAAAGAG TTGGTAGCTC 
     5881 TTGATCCGGC AAACAAACCA CCGCTGGTAG CGGTGGTTTT TTTGTTTGCA AGCAGCAGAT 
     5941 TACGCGCAGA AAAAAAGGAT CTCAAGAAGA TCCTTTGATC TTTTCTACGG GGTCTGACGC 
     6001 TCAGTGGAAC GAAAACTCAC GTTAAGGGAT TTTGGTCATG AGATTATCAA AAAGGATCTT 
     6061 CACCTAGATC CTTTTAAATT AAAAATGAAG TTTTAAATCA ATCTAAAGTA TATATGAGTA 
     6121 ACCTGAGGCT ATGGCAGGGC CTGCCGCCCC GACGTTGGCT GCGAGCCCTG GGCCTTCACC 
     6181 CGAACTTGGG GGGTGGGGTG GGGAAAAGGA AGAAACGCGG GCGTATTGGC CCCAATGGGG 
     6241 TCTCGGTGGG GTATCGACAG AGTGCCAGCC CTGGGACCGA ACCCCGCGTT TATGAACAAA 
     6301 CGACCCAACA CCGTGCGTTT TATTCTGTCT TTTTATTGCC GTCATAGCGC GGGTTCCTTC 
     6361 CGGTATTGTC TCCTTCCGTG TTTCAGTTAG CCTCCCCCTA GGGTGGGCGA AGAACTCCAG 
     6421 CATGAGATCC CCGCGCTGGA GGATCATCCA GCCGGCGTCC CGGAAAACGA TTCCGAAGCC 
     6481 CAACCTTTCA TAGAAGGCGG CGGTGGAATC GAAATCTCGT GATGGCAGGT TGGGCGTCGC 
     6541 TTGGTCGGTC ATTTCGAACC CCAGAGTCCC GCTCAGAAGA ACTCGTCAAG AAGGCGATAG 
     6601 AAGGCGATGC GCTGCGAATC GGGAGCGGCG ATACCGTAAA GCACGAGGAA GCGGTCAGCC 
     6661 CATTCGCCGC CAAGCTCTTC AGCAATATCA CGGGTAGCCA ACGCTATGTC CTGATAGCGA 
     6721 TCCGCCACAC CCAGCCGGCC ACAGTCGATG AATCCAGAAA AGCGGCCATT TTCCACCATG 
     6781 ATATTCGGCA AGCAGGCATC GCCATGGGTC ACGACGAGAT CCTCGCCGTC GGGCATGCTC 
     6841 GCCTTGAGCC TGGCGAACAG TTCGGCTGGC GCGAGCCCCT GATGCTCTTC GTCCAGATCA 
     6901 TCCTGATCGA CAAGACCGGC TTCCATCCGA GTACGTGCTC GCTCGATGCG ATGTTTCGCT 
     6961 TGGTGGTCGA ATGGGCAGGT AGCCGGATCA AGCGTATGCA GCCGCCGCAT TGCATCAGCC 
     7021 ATGATGGATA CTTTCTCGGC AGGAGCAAGG TGAGATGACA GGAGATCCTG CCCCGGCACT 
     7081 TCGCCCAATA GCAGCCAGTC CCTTCCCGCT TCAGTGACAA CGTCGAGCAC AGCTGCGCAA 
     7141 GGAACGCCCG TCGTGGCCAG CCACGATAGC CGCGCTGCCT CGTCTTGCAG TTCATTCAGG 
     7201 GCACCGGACA GGTCGGTCTT GACAAAAAGA ACCGGGCGCC CCTGCGCTGA CAGCCGGAAC 
     7261 ACGGCGGCAT CAGAGCAGCC GATTGTCTGT TGTGCCCAGT CATAGCCGAA TAGCCTCTCC 
     7321 ACCCAAGCGG CCGGAGAACC TGCGTGCAAT CCATCTTGTT CAATCATGCG AAACGATCCT 
     7381 CATCCTGTCT CTTGATCGAT CTTTGCAAAA GCCTAGGCCT CCAAAAAAGC CTCCTCACTA 
     7441 CTTCTGGAAT AGCTCAGAGG CCGAGGCGGC CTCGGCCTCT GCATAAATAA AAAAAATTAG 
     7501 TCAGCCATGG GGCGGAGAAT GGGCGGAACT GGGCGGAGTT AGGGGCGGGA TGGGCGGAGT 
     7561 TAGGGGCGGG ACTATGGTTG CTGACTAATT GAGATGCATG CTTTGCATAC TTCTGCCTGC 
     7621 TGGGGAGCCT GGGGACTTTC CACACCTGGT TGCTGACTAA TTGAGATGCA TGCTTTGCAT 
     7681 ACTTCTGCCT GCTGGGGAGC CTGGGGACTT TCCACACCCT AACTGACACA CATTCCACAG 
     7741 CTGGTTCTTT CCGCCTCAGG ACTCTTCCTT TTTCAATATT ATTGAAGCAT TTATCAGGGT 
     7801 TATTGTCTCA TGAGCGGATA CATATTTGAA TGTATTTAGA AAAATAAACA AATAGGGGTT 
     7861 CCGCGCACAT TTCCCCGAAA AGTGCCACCT GACGCGCCCT GTAGCGGCGC ATTAAGCGCG 
     7921 GCGGGTGTGG TGGTTACGCG CAGCGTGACC GCTACACTTG CCAGCGCCCT AGCGCCCGCT 
     7981 CCTTTCGCTT TCTTCCCTTC CTTTCTCGCC ACGTTCGCCG GCTTTCCCCG TCAAGCTCTA 
     8041 AATCGGGGGC TCCCTTTAGG GTTCCGATTT AGTGCTTTAC GGCACCTCGA CCCCAAAAAA 
     8101 CTTGATTAGG GTGATGGTTC ACGTAGTGGG CCATCGCCCT GATAGACGGT TTTTCGCCCT 
     8161 TTGACGTTGG AGTCCACGTT CTTTAATAGT GGACTCTTGT TCCAAACTGG AACAACACTC 
     8221 AACCCTATCT CGGTCTATTC TTTTGATTTA TAAGGGATTT TGCCGATTTC GGCCTATTGG 
     8281 TTAAAAAATG AGCTGATTTA ACAAAAATTT AACGCGAATT TT
//