LOCUS       RR212194                6924 bp    DNA     circular  SYN 
DEFINITION  Srpk2 (Myc-DDK-tagged ORF) - Rat SFRS protein kinase 2 (Srpk2), (10 ug)
ACCESSION   NM_001106575
VERSION     NM_001106575.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3081..3172 
                     /label="Myc-DDK"  
     misc_feature    3202..3787 
                     /label="PolyA signal"  
     misc_feature    3259..3278 
                     /label="Restriction Enzyme"  
     misc_feature    4063..4735 
                     /label="ColE1"  
     misc_feature    5165..5959 
                     /label="Kan/Neo"  
     misc_feature    5994..6296 
                     /label="SV40 ori"  
     misc_feature    6382..6859 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3063..3069 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3063 
                     /label="Srpk2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCAGTTAAC TCTGAGAAGT CGTCCTCTTC AGAAAGGCCG GAGCCTCAAC 
     1081 AGAAAGCTCC TTTAGTTCCT CCTCCTCCAC CACCGCCACC GCCACCACCA CTGCCAGACC 
     1141 CCGTGCCTCC AGAGCCAGAG GAGGAGATTC TGGGGTCAGA TGATGAGGAG CAGGAGGACC 
     1201 CCGCGGATTA CTGCAAAGGT GGCTATCATC CAGTGAAAAT CGGAGACCTC TTCAATGGTC 
     1261 GCTATCATGT CATTAGGAAG CTAGGATGGG GGCACTTTTC CACCGTCTGG CTATGCTGGG 
     1321 ATATGCAAGG GAAGAGGTTT GTCGCCATGA AAGTTGTAAA AAGTGCACAG CATTATACAG 
     1381 AGACAGCCTT GGATGAAATT AAATTGCTCA AATGTGTTCG AGAAAGTGAC CCCAGTGACC 
     1441 CAAACAAAGA CATGGTAGTC CAGCTAATCG ATGACTTCAA GATCTCAGGC ATGAATGGGA 
     1501 TACATGTCTG CATGGTCTTC GAAGTACTTG GTCACCATCT CCTCAAATGG ATCATCAAGT 
     1561 CCAACTATCA AGGCCTCCCA GTCCGCTGTG TGAAGAGTAT CATTCGACAG GTCCTTCAGG 
     1621 GCTTAGACTA TCTACACAGC AAGTGCAAGA TAATCCACAC TGATATCAAG CCGGAGAACA 
     1681 TCCTGATGTG TGTGGACGGC GCGTACGTGC GGAGAATGGC GGCCGAGGCT ACGGAGTGGC 
     1741 AGAAAGCAGG TGCTCCTCCT CCTTCTGGGT CTGCAGTGAG TACGGCTCCA CAGCAGAAAC 
     1801 CTATAGGAAA AATATCTAAA AACAAAAAGA AAAAGCTAAA AAAGAAACAG AAGAGGCAGG 
     1861 CTGAGTTGCT GGAGAAGCGC CTGCAGGAGA TTGAGGAATT GGAGAGAGAA GCCGAAAGGA 
     1921 AAATCCTAGA AGAGAACACC ACCTCTGCAG AAGCTTCCAG TGAGCAAGAT GGAGAATGCC 
     1981 AGCCTGAGGT GAAACTGAAA GAGGCCGAGT TAGAGGACAC AACTGAGGAA GAGACAGCAA 
     2041 AGGACAATGG TGAAGTTGAA GACCAGGAAG AGAAAGAAGA CACAGAGAAG GAAAACACTG 
     2101 AGAAGGATGA AGATGATGTT GAACAGGAAC TTGAGAACAT AGACCCTACG TGGATGGAGT 
     2161 CCCCTAAGGC CAACGGCCAT ATTGAAAATG GCCTGTTTTT ACTGGAGCAG CAGCTGGAGG 
     2221 ATGAAGAGGA TGAGGAAGAT GACTGTGCAA ACCCCGAGGA GTATAACCTG GATGAACCAA 
     2281 ATGCGGAGAG TGATTATACG TATAGCAGCT CCTATGAACA ATTCAATGGT GAATTGCCAA 
     2341 ACGGACAGCC TAAGAGTCCA GAGTTTCCCA CACCGCTGTT TTCTGGGCCC TTAGAACCTG 
     2401 TGGCCTCTGA CTCTGTGCTG TCAGAGGGAT CACCACTCAC CGAGCCAGAG GAAAGCAGTC 
     2461 CATCCCGTGA CAGAAGCAGG ACAGTTTCAG CCTCCAGTAC TGGAGACTTG CCAAAAACAA 
     2521 AAACCCGGGC AGCTGACCTG TTGGTGAACC CTCTGGATCC ACAGAATGCA GATAAAATTA 
     2581 GAGTAAAAAT TGCTGACCTG GGAAATGCTT GTTGGGTGCA TAAACATTTC ACAGAGGATA 
     2641 TCCAAACACG TCAGTATAGG TCCATAGAGG TTTTGATAGG AGCAGGCTAC AGTACACCTG 
     2701 CAGACATTTG GAGTACAGCA TGCATGGCAT TTGAGCTGGC AACAGGAGAC TATTTGTTCG 
     2761 AACCGCATTC TGGGGAAGAC TATTCCAGAG ATGAAGACCA CATAGCCCAC ATCATAGAGC 
     2821 TGCTAGGCAG TATCCCAAGG CACTTTGCTC TGTCTGGAAA ATACTCCCGG GAGTTCTTCA 
     2881 ACCGCAGAGG AGAACTGCGG CACATCACCA AGCTGAAGCC CTGGAGCCTC TTTGATGTTC 
     2941 TTGTGGAAAA GTATGGCTGG CCCCATGAAG ATGCTGCACA GTTTACAGAT TTCCTGATCC 
     3001 CAATGTTAGA GATGGTTCCA GAAAAACGAG CCTCAGCTGG CGAATGCCTT CGGCATCCTT 
     3061 GGTTGAATTC TACGCGTACG CGGCCGCTCG AGCAGAAACT CATCTCAGAA GAGGATCTGG 
     3121 CAGCAAATGA TATCCTGGAT TACAAGGATG ACGACGATAA GGTTTAAACG GCCGGCCGCG 
     3181 GTCATAGCTG TTTCCTGAAC AGATCCCGGG TGGCATCCCT GTGACCCCTC CCCAGTGCCT 
     3241 CTCCTGGCCC TGGAAGTTGC CACTCCAGTG CCCACCAGCC TTGTCCTAAT AAAATTAAGT 
     3301 TGCATCATTT TGTCTGACTA GGTGTCCTTC TATAATATTA TGGGGTGGAG GGGGGTGGTA 
     3361 TGGAGCAAGG GGCAAGTTGG GAAGACAACC TGTAGGGCCT GCGGGGTCTA TTGGGAACCA 
     3421 AGCTGGAGTG CAGTGGCACA ATCTTGGCTC ACTGCAATCT CCGCCTCCTG GGTTCAAGCG 
     3481 ATTCTCCTGC CTCAGCCTCC CGAGTTGTTG GGATTCCAGG CATGCATGAC CAGGCTCAGC 
     3541 TAATTTTTGT TTTTTTGGTA GAGACGGGGT TTCACCATAT TGGCCAGGCT GGTCTCCAAC 
     3601 TCCTAATCTC AGGTGATCTA CCCACCTTGG CCTCCCAAAT TGCTGGGATT ACAGGCGTGA 
     3661 ACCACTGCTC CCTTCCCTGT CCTTCTGATT TTAAAATAAC TATACCAGCA GGAGGACGTC 
     3721 CAGACACAGC ATAGGCTACC TGGCCATGCC CAACCGGTGG GACATTTGAG TTGCTTGCTT 
     3781 GGCACTGTCC TCTCATGCGT TGGGTCCACT CAGTAGATGC CTGTTGAATT GGGTACGCGG 
     3841 CCAGCGGCGA GCGGTATCAG CTCACTCAAA GGCGGTAATA CGGTTATCCA CAGAATCAGG 
     3901 GGATAACGCA GGAAAGAACA TGTGAGCAAA AGGCCAGCAA AAGGCCAGGA ACCGTAAAAA 
     3961 GGCCGCGTTG CTGGCGTTTT TCCATAGGCT CCGCCCCCCT GACGAGCATC ACAAAAATCG 
     4021 ACGCTCAAGT CAGAGGTGGC GAAACCCGAC AGGACTATAA AGATACCAGG CGTTTCCCCC 
     4081 TGGAAGCTCC CTCGTGCGCT CTCCTGTTCC GACCCTGCCG CTTACCGGAT ACCTGTCCGC 
     4141 CTTTCTCCCT TCGGGAAGCG TGGCGCTTTC TCATAGCTCA CGCTGTAGGT ATCTCAGTTC 
     4201 GGTGTAGGTC GTTCGCTCCA AGCTGGGCTG TGTGCACGAA CCCCCCGTTC AGCCCGACCG 
     4261 CTGCGCCTTA TCCGGTAACT ATCGTCTTGA GTCCAACCCG GTAAGACACG ACTTATCGCC 
     4321 ACTGGCAGCA GCCACTGGTA ACAGGATTAG CAGAGCGAGG TATGTAGGCG GTGCTACAGA 
     4381 GTTCTTGAAG TGGTGGCCTA ACTACGGCTA CACTAGAAGA ACAGTATTTG GTATCTGCGC 
     4441 TCTGCTGAAG CCAGTTACCT TCGGAAAAAG AGTTGGTAGC TCTTGATCCG GCAAACAAAC 
     4501 CACCGCTGGT AGCGGTGGTT TTTTTGTTTG CAAGCAGCAG ATTACGCGCA GAAAAAAAGG 
     4561 ATCTCAAGAA GATCCTTTGA TCTTTTCTAC GGGGTCTGAC GCTCAGTGGA ACGAAAACTC 
     4621 ACGTTAAGGG ATTTTGGTCA TGAGATTATC AAAAAGGATC TTCACCTAGA TCCTTTTAAA 
     4681 TTAAAAATGA AGTTTTAAAT CAATCTAAAG TATATATGAG TAACCTGAGG CTATGGCAGG 
     4741 GCCTGCCGCC CCGACGTTGG CTGCGAGCCC TGGGCCTTCA CCCGAACTTG GGGGGTGGGG 
     4801 TGGGGAAAAG GAAGAAACGC GGGCGTATTG GCCCCAATGG GGTCTCGGTG GGGTATCGAC 
     4861 AGAGTGCCAG CCCTGGGACC GAACCCCGCG TTTATGAACA AACGACCCAA CACCGTGCGT 
     4921 TTTATTCTGT CTTTTTATTG CCGTCATAGC GCGGGTTCCT TCCGGTATTG TCTCCTTCCG 
     4981 TGTTTCAGTT AGCCTCCCCC TAGGGTGGGC GAAGAACTCC AGCATGAGAT CCCCGCGCTG 
     5041 GAGGATCATC CAGCCGGCGT CCCGGAAAAC GATTCCGAAG CCCAACCTTT CATAGAAGGC 
     5101 GGCGGTGGAA TCGAAATCTC GTGATGGCAG GTTGGGCGTC GCTTGGTCGG TCATTTCGAA 
     5161 CCCCAGAGTC CCGCTCAGAA GAACTCGTCA AGAAGGCGAT AGAAGGCGAT GCGCTGCGAA 
     5221 TCGGGAGCGG CGATACCGTA AAGCACGAGG AAGCGGTCAG CCCATTCGCC GCCAAGCTCT 
     5281 TCAGCAATAT CACGGGTAGC CAACGCTATG TCCTGATAGC GATCCGCCAC ACCCAGCCGG 
     5341 CCACAGTCGA TGAATCCAGA AAAGCGGCCA TTTTCCACCA TGATATTCGG CAAGCAGGCA 
     5401 TCGCCATGGG TCACGACGAG ATCCTCGCCG TCGGGCATGC TCGCCTTGAG CCTGGCGAAC 
     5461 AGTTCGGCTG GCGCGAGCCC CTGATGCTCT TCGTCCAGAT CATCCTGATC GACAAGACCG 
     5521 GCTTCCATCC GAGTACGTGC TCGCTCGATG CGATGTTTCG CTTGGTGGTC GAATGGGCAG 
     5581 GTAGCCGGAT CAAGCGTATG CAGCCGCCGC ATTGCATCAG CCATGATGGA TACTTTCTCG 
     5641 GCAGGAGCAA GGTGAGATGA CAGGAGATCC TGCCCCGGCA CTTCGCCCAA TAGCAGCCAG 
     5701 TCCCTTCCCG CTTCAGTGAC AACGTCGAGC ACAGCTGCGC AAGGAACGCC CGTCGTGGCC 
     5761 AGCCACGATA GCCGCGCTGC CTCGTCTTGC AGTTCATTCA GGGCACCGGA CAGGTCGGTC 
     5821 TTGACAAAAA GAACCGGGCG CCCCTGCGCT GACAGCCGGA ACACGGCGGC ATCAGAGCAG 
     5881 CCGATTGTCT GTTGTGCCCA GTCATAGCCG AATAGCCTCT CCACCCAAGC GGCCGGAGAA 
     5941 CCTGCGTGCA ATCCATCTTG TTCAATCATG CGAAACGATC CTCATCCTGT CTCTTGATCG 
     6001 ATCTTTGCAA AAGCCTAGGC CTCCAAAAAA GCCTCCTCAC TACTTCTGGA ATAGCTCAGA 
     6061 GGCCGAGGCG GCCTCGGCCT CTGCATAAAT AAAAAAAATT AGTCAGCCAT GGGGCGGAGA 
     6121 ATGGGCGGAA CTGGGCGGAG TTAGGGGCGG GATGGGCGGA GTTAGGGGCG GGACTATGGT 
     6181 TGCTGACTAA TTGAGATGCA TGCTTTGCAT ACTTCTGCCT GCTGGGGAGC CTGGGGACTT 
     6241 TCCACACCTG GTTGCTGACT AATTGAGATG CATGCTTTGC ATACTTCTGC CTGCTGGGGA 
     6301 GCCTGGGGAC TTTCCACACC CTAACTGACA CACATTCCAC AGCTGGTTCT TTCCGCCTCA 
     6361 GGACTCTTCC TTTTTCAATA TTATTGAAGC ATTTATCAGG GTTATTGTCT CATGAGCGGA 
     6421 TACATATTTG AATGTATTTA GAAAAATAAA CAAATAGGGG TTCCGCGCAC ATTTCCCCGA 
     6481 AAAGTGCCAC CTGACGCGCC CTGTAGCGGC GCATTAAGCG CGGCGGGTGT GGTGGTTACG 
     6541 CGCAGCGTGA CCGCTACACT TGCCAGCGCC CTAGCGCCCG CTCCTTTCGC TTTCTTCCCT 
     6601 TCCTTTCTCG CCACGTTCGC CGGCTTTCCC CGTCAAGCTC TAAATCGGGG GCTCCCTTTA 
     6661 GGGTTCCGAT TTAGTGCTTT ACGGCACCTC GACCCCAAAA AACTTGATTA GGGTGATGGT 
     6721 TCACGTAGTG GGCCATCGCC CTGATAGACG GTTTTTCGCC CTTTGACGTT GGAGTCCACG 
     6781 TTCTTTAATA GTGGACTCTT GTTCCAAACT GGAACAACAC TCAACCCTAT CTCGGTCTAT 
     6841 TCTTTTGATT TATAAGGGAT TTTGCCGATT TCGGCCTATT GGTTAAAAAA TGAGCTGATT 
     6901 TAACAAAAAT TTAACGCGAA TTTT
//