LOCUS       RR212189                8379 bp    DNA     circular  SYN 
DEFINITION  Adcy6 (Myc-DDK-tagged ORF) - Rat adenylate cyclase 6 (Adcy6), (10 ug)
ACCESSION   NM_012821
VERSION     NM_012821.4
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4536..4627 
                     /label="Myc-DDK"  
     misc_feature    4657..5242 
                     /label="PolyA signal"  
     misc_feature    4714..4733 
                     /label="Restriction Enzyme"  
     misc_feature    5518..6190 
                     /label="ColE1"  
     misc_feature    6620..7414 
                     /label="Kan/Neo"  
     misc_feature    7449..7751 
                     /label="SV40 ori"  
     misc_feature    7837..8314 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4518..4524 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4518 
                     /label="Adcy6"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GTCATGGTTT AGCGGCCTCC TGGTTCCCAA AGTGGATGAA CGGAAAACAG 
     1081 CCTGGGGCGA ACGCAATGGA CAGAAGCGCC CACGCCAGGC GACCCGAGCC CGTGGCTTCT 
     1141 GCGCGCCCCG CTACATGAGC TGCCTCAAGA ATGTGGAGCC ACCCAGCCCC ACTCCTGCAG 
     1201 CTCGCACTCG GTGCCCCTGG CAGGATGAAG CCTTCATCAG GAGGGCTGGC CCGGGAAGGG 
     1261 GTGTGAAGCT GGGGCTGCGG TCAGTGGCCT TGGGTTTTGA TGACACTGAG GTGACCACAC 
     1321 CGATGGGGAC AGCTGAAGTG GCACCCGACA CATCGCCTCG AAGCGGTCCG TCCTGCTGGC 
     1381 ACCGGCTAGC GCAGGTGTTC CAGTCTAAGC AGTTCCGCTC CGCCAAGCTG GAGCGTCTGT 
     1441 ACCAGCGGTA CTTCTTCCAG ATGAACCAGA GCAGCCTCAC GCTGCTCATG GCGGTGCTTG 
     1501 TGCTCCTCAT GGCTGTACTG TTGACCTTCC ACGCCGCGCC TGCCCTGCCT CAGCCTGCTT 
     1561 ATGTGGCCCT GCTGACCTGT GCCTCCGTCC TTTTTGTGGT ACTCATGGTA GTGTGTAACC 
     1621 GACATAGCTT CCGCCAGGAC TCCATGTGGG TAGTGAGCTA TGTGGTTCTG GGCATCCTAG 
     1681 CAGCCGTGCA AGTCGGGGGT GCCCTGGCAG CCAACCCACG CAGCCCCTCA GCAGGCCTTT 
     1741 GGTGCCCCGT GTTCTTCGTC TACATCACCT ACACACTTCT TCCCATTCGT ATGCGAGCGG 
     1801 CCGTGCTCAG TGGCCTGGGT CTTTCCACCC TGCATTTGAT TTTGGCCTGG CATCTCAACA 
     1861 ATGGTGACCC CTTCCTTTGG AAGCAGCTCG GTGCTAACGT GGTGCTCTTC CTGTGCACCA 
     1921 ATGCCATCGG TGTCTGCACG CACTACCCCG CTGAAGTGTC TCAGCGCCAA GCCTTTCAGG 
     1981 AGACCCGTGG TTACATCCAG GCCCGGCTGC ACTTGCAGCA TGAGAATCGA CAGCAGGAAC 
     2041 GGCTGCTGCT GTCGGTGTTG CCCCAGCATG TTGCCATGGA AATGAAAGAG GATATCAACA 
     2101 CAAAAAAGGA AGACATGATG TTCCACAAGA TTTACATCCA GAAGCATGAC AATGTCAGCA 
     2161 TCCTGTTTGC CGACATCGAG GGCTTCACCA GCCTGGCCTC CCAGTGCACT GCCCAGGAAC 
     2221 TGGTCATGAC CTTGAATGAG CTCTTTGCCC GGTTCGACAA GCTGGCTGCG GAGAATCACT 
     2281 GTCTGAGGAT CAAGATCTTA GGAGACTGTT ACTACTGTGT GTCGGGGCTG CCGGAGGCCC 
     2341 GGGCAGACCA TGCCCACTGC TGTGTGGAGA TGGGGGTAGA CATGATCGAG GCCATCTCGC 
     2401 TGGTGCGTGA GGTAACGGGT GTAAATGTGA ACATGCGCGT GGGCATCCAC AGCGGGCGTG 
     2461 TACACTGCGG TGTCCTTGGT CTGCGGAAAT GGCAGTTTGA TGTCTGGTCC AACGATGTGA 
     2521 CCCTGGCCAA CCACATGGAG GCGGGGGGCC GGGCCGGCCG CATCCACATC ACTCGGGCCA 
     2581 CACTGCAGTA CCTGAACGGG GACTATGAGG TGGAGCCAGG CCGTGGCGGT GAGCGCAACG 
     2641 GCTACCTCAA GGAGCAGTGC ATTGAGACCT TCCTCATACT AGGAGCCAGC CAGAAACGGA 
     2701 AAGAGGAGAA GGCCATGCTG GTCAAGCTGC AGCGGACGCG GGCCAACTCC ATGGAAGGAC 
     2761 TGATGCCCCG CTGGGTTCCT GACCGTGCCT TCTCCCGGAC CAAGGACTCT AAGGCATTCC 
     2821 GACAGATGGG CATCGATGAC TCTAGCAAAG AGAACCGGGG TGCCCAAGAT GCTCTGAACC 
     2881 CTGAGGATGA GGTGGACGAG TTTCTGGGCC GAGCCATCGA TGCCCGAAGC ATCGACCAGC 
     2941 TGCGTAAGGA CCATGTGCGC CGGTTCCTGC TCACCTTCCA GAGGGAGGAT CTCGAGAAGA 
     3001 AGTATTCACG GAAAGTAGAC CCTCGTTTCG GAGCCTACGT CGCCTGTGCC CTCCTGGTTT 
     3061 TCTGCTTCAT CTGTTTCATC CAGTTCCTCG TATTCCCACA CTCCGCCCTG ATACTCGGGA 
     3121 TTTATGCCGG GATCTTCCTT TTGCTGCTGG TCACCGTGCT CATCTGTGCT GTGTGCTCCT 
     3181 GTGGGTCTTT CTTCCCCAAC GCCCTGCAGC GCCTGTCCCG CAGTATCGTC CGCTCACGGG 
     3241 TGCACAGCAC GGCTGTTGGA GTCTTCTCGG TTCTGCTTGT GTTCATCTCT GCCATTGCCA 
     3301 ACATGTTCAC CTGCAGTCAC ACCCCACTGA GGACCTGTGC GGCCCGGATG CTGAACTTAA 
     3361 CACCGTCCGA TGTCACCGCC TGTCACCTAC GACAGATCAA TTACTCTCTG GGACTGGAAG 
     3421 CTCCCCTGTG TGAGGGCACA GCACCCACCT GCAGTTTCCC TGAGTACTTT GTCGGGAGTG 
     3481 TGCTGTTGAG TCTCTTGGCC AGCTCCGTCT TCCTCCACAT CAGCAGCATT GGCAAGCTAG 
     3541 TTATGACCTT TGTCTTGGGG TTCATCTACT TGCTTCTGCT TTTGCTGGGT CCCCCAGCCA 
     3601 CCATCTTTGA CAACTATGAT CTACTGCTTA GCGTCCATGG CTTGGCTTCC TCCAATGAGA 
     3661 CCTTCGATGG GCTGGACTGC CCAGCCGTAG GGAGGGTAGC GCTCAAATAC ATGACCCCTG 
     3721 TGATTCTCCT CGTGTTCGCC CTGGCACTGT ATCTACACGC ACAACAGGTG GAATCTACCG 
     3781 CCCGCCTGGA CTTCCTGTGG AAACTGCAGG CCACAGGGGA GAAGGAGGAG ATGGAGGAGT 
     3841 TGCAGGCCTA CAACCGGCGG CTGCTGCATA ACATCCTTCC CAAGGACGTG GCTGCCCACT 
     3901 TCCTGGCCCG GGAGCGCCGC AACGACGAGC TGTACTACCA ATCCTGCGAG TGCGTGGCTG 
     3961 TCATGTTTGC CTCCATCGCC AACTTCTCTG AGTTCTATGT GGAACTGGAG GCGAACAATG 
     4021 AGGGCGTGGA GTGCCTGCGA CTGCTCAATG AGATCATCGC GGACTTTGAT GAGATCATCA 
     4081 GTGAGGAGAG GTTCCGGCAG CTGGAGAAGA TCAAGACCAT CGGTAGCACT TACATGGCCG 
     4141 CCTCCGGGCT AAATGCCAGC ACCTATGACC AGGTCGGCCG CTCGCACATC ACCGCCCTGG 
     4201 CAGACTACGC CATGCGGCTT ATGGAGCAAA TGAAACACAT CAACGAACAC TCTTTCAACA 
     4261 ACTTCCAGAT GAAGATCGGG TTGAACATGG GTCCGGTTGT AGCAGGTGTC ATTGGGGCCC 
     4321 GGAAGCCACA GTATGACATC TGGGGAAACA CGGTGAATGT TTCCAGCCGT ATGGACAGCA 
     4381 CAGGAGTTCC TGACCGAATA CAGGTGACCA CGGATCTCTA CCAGGTTCTA GCTGCCAAGG 
     4441 GCTACCAACT GGAGTGTCGA GGGGTGGTCA AGGTGAAGGG AAAGGGGGAG ATGACCACCT 
     4501 ACTTCCTCAA TGGGGGCCCC AGCAGTACGC GTACGCGGCC GCTCGAGCAG AAACTCATCT 
     4561 CAGAAGAGGA TCTGGCAGCA AATGATATCC TGGATTACAA GGATGACGAC GATAAGGTTT 
     4621 AAACGGCCGG CCGCGGTCAT AGCTGTTTCC TGAACAGATC CCGGGTGGCA TCCCTGTGAC 
     4681 CCCTCCCCAG TGCCTCTCCT GGCCCTGGAA GTTGCCACTC CAGTGCCCAC CAGCCTTGTC 
     4741 CTAATAAAAT TAAGTTGCAT CATTTTGTCT GACTAGGTGT CCTTCTATAA TATTATGGGG 
     4801 TGGAGGGGGG TGGTATGGAG CAAGGGGCAA GTTGGGAAGA CAACCTGTAG GGCCTGCGGG 
     4861 GTCTATTGGG AACCAAGCTG GAGTGCAGTG GCACAATCTT GGCTCACTGC AATCTCCGCC 
     4921 TCCTGGGTTC AAGCGATTCT CCTGCCTCAG CCTCCCGAGT TGTTGGGATT CCAGGCATGC 
     4981 ATGACCAGGC TCAGCTAATT TTTGTTTTTT TGGTAGAGAC GGGGTTTCAC CATATTGGCC 
     5041 AGGCTGGTCT CCAACTCCTA ATCTCAGGTG ATCTACCCAC CTTGGCCTCC CAAATTGCTG 
     5101 GGATTACAGG CGTGAACCAC TGCTCCCTTC CCTGTCCTTC TGATTTTAAA ATAACTATAC 
     5161 CAGCAGGAGG ACGTCCAGAC ACAGCATAGG CTACCTGGCC ATGCCCAACC GGTGGGACAT 
     5221 TTGAGTTGCT TGCTTGGCAC TGTCCTCTCA TGCGTTGGGT CCACTCAGTA GATGCCTGTT 
     5281 GAATTGGGTA CGCGGCCAGC GGCGAGCGGT ATCAGCTCAC TCAAAGGCGG TAATACGGTT 
     5341 ATCCACAGAA TCAGGGGATA ACGCAGGAAA GAACATGTGA GCAAAAGGCC AGCAAAAGGC 
     5401 CAGGAACCGT AAAAAGGCCG CGTTGCTGGC GTTTTTCCAT AGGCTCCGCC CCCCTGACGA 
     5461 GCATCACAAA AATCGACGCT CAAGTCAGAG GTGGCGAAAC CCGACAGGAC TATAAAGATA 
     5521 CCAGGCGTTT CCCCCTGGAA GCTCCCTCGT GCGCTCTCCT GTTCCGACCC TGCCGCTTAC 
     5581 CGGATACCTG TCCGCCTTTC TCCCTTCGGG AAGCGTGGCG CTTTCTCATA GCTCACGCTG 
     5641 TAGGTATCTC AGTTCGGTGT AGGTCGTTCG CTCCAAGCTG GGCTGTGTGC ACGAACCCCC 
     5701 CGTTCAGCCC GACCGCTGCG CCTTATCCGG TAACTATCGT CTTGAGTCCA ACCCGGTAAG 
     5761 ACACGACTTA TCGCCACTGG CAGCAGCCAC TGGTAACAGG ATTAGCAGAG CGAGGTATGT 
     5821 AGGCGGTGCT ACAGAGTTCT TGAAGTGGTG GCCTAACTAC GGCTACACTA GAAGAACAGT 
     5881 ATTTGGTATC TGCGCTCTGC TGAAGCCAGT TACCTTCGGA AAAAGAGTTG GTAGCTCTTG 
     5941 ATCCGGCAAA CAAACCACCG CTGGTAGCGG TGGTTTTTTT GTTTGCAAGC AGCAGATTAC 
     6001 GCGCAGAAAA AAAGGATCTC AAGAAGATCC TTTGATCTTT TCTACGGGGT CTGACGCTCA 
     6061 GTGGAACGAA AACTCACGTT AAGGGATTTT GGTCATGAGA TTATCAAAAA GGATCTTCAC 
     6121 CTAGATCCTT TTAAATTAAA AATGAAGTTT TAAATCAATC TAAAGTATAT ATGAGTAACC 
     6181 TGAGGCTATG GCAGGGCCTG CCGCCCCGAC GTTGGCTGCG AGCCCTGGGC CTTCACCCGA 
     6241 ACTTGGGGGG TGGGGTGGGG AAAAGGAAGA AACGCGGGCG TATTGGCCCC AATGGGGTCT 
     6301 CGGTGGGGTA TCGACAGAGT GCCAGCCCTG GGACCGAACC CCGCGTTTAT GAACAAACGA 
     6361 CCCAACACCG TGCGTTTTAT TCTGTCTTTT TATTGCCGTC ATAGCGCGGG TTCCTTCCGG 
     6421 TATTGTCTCC TTCCGTGTTT CAGTTAGCCT CCCCCTAGGG TGGGCGAAGA ACTCCAGCAT 
     6481 GAGATCCCCG CGCTGGAGGA TCATCCAGCC GGCGTCCCGG AAAACGATTC CGAAGCCCAA 
     6541 CCTTTCATAG AAGGCGGCGG TGGAATCGAA ATCTCGTGAT GGCAGGTTGG GCGTCGCTTG 
     6601 GTCGGTCATT TCGAACCCCA GAGTCCCGCT CAGAAGAACT CGTCAAGAAG GCGATAGAAG 
     6661 GCGATGCGCT GCGAATCGGG AGCGGCGATA CCGTAAAGCA CGAGGAAGCG GTCAGCCCAT 
     6721 TCGCCGCCAA GCTCTTCAGC AATATCACGG GTAGCCAACG CTATGTCCTG ATAGCGATCC 
     6781 GCCACACCCA GCCGGCCACA GTCGATGAAT CCAGAAAAGC GGCCATTTTC CACCATGATA 
     6841 TTCGGCAAGC AGGCATCGCC ATGGGTCACG ACGAGATCCT CGCCGTCGGG CATGCTCGCC 
     6901 TTGAGCCTGG CGAACAGTTC GGCTGGCGCG AGCCCCTGAT GCTCTTCGTC CAGATCATCC 
     6961 TGATCGACAA GACCGGCTTC CATCCGAGTA CGTGCTCGCT CGATGCGATG TTTCGCTTGG 
     7021 TGGTCGAATG GGCAGGTAGC CGGATCAAGC GTATGCAGCC GCCGCATTGC ATCAGCCATG 
     7081 ATGGATACTT TCTCGGCAGG AGCAAGGTGA GATGACAGGA GATCCTGCCC CGGCACTTCG 
     7141 CCCAATAGCA GCCAGTCCCT TCCCGCTTCA GTGACAACGT CGAGCACAGC TGCGCAAGGA 
     7201 ACGCCCGTCG TGGCCAGCCA CGATAGCCGC GCTGCCTCGT CTTGCAGTTC ATTCAGGGCA 
     7261 CCGGACAGGT CGGTCTTGAC AAAAAGAACC GGGCGCCCCT GCGCTGACAG CCGGAACACG 
     7321 GCGGCATCAG AGCAGCCGAT TGTCTGTTGT GCCCAGTCAT AGCCGAATAG CCTCTCCACC 
     7381 CAAGCGGCCG GAGAACCTGC GTGCAATCCA TCTTGTTCAA TCATGCGAAA CGATCCTCAT 
     7441 CCTGTCTCTT GATCGATCTT TGCAAAAGCC TAGGCCTCCA AAAAAGCCTC CTCACTACTT 
     7501 CTGGAATAGC TCAGAGGCCG AGGCGGCCTC GGCCTCTGCA TAAATAAAAA AAATTAGTCA 
     7561 GCCATGGGGC GGAGAATGGG CGGAACTGGG CGGAGTTAGG GGCGGGATGG GCGGAGTTAG 
     7621 GGGCGGGACT ATGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC TGCCTGCTGG 
     7681 GGAGCCTGGG GACTTTCCAC ACCTGGTTGC TGACTAATTG AGATGCATGC TTTGCATACT 
     7741 TCTGCCTGCT GGGGAGCCTG GGGACTTTCC ACACCCTAAC TGACACACAT TCCACAGCTG 
     7801 GTTCTTTCCG CCTCAGGACT CTTCCTTTTT CAATATTATT GAAGCATTTA TCAGGGTTAT 
     7861 TGTCTCATGA GCGGATACAT ATTTGAATGT ATTTAGAAAA ATAAACAAAT AGGGGTTCCG 
     7921 CGCACATTTC CCCGAAAAGT GCCACCTGAC GCGCCCTGTA GCGGCGCATT AAGCGCGGCG 
     7981 GGTGTGGTGG TTACGCGCAG CGTGACCGCT ACACTTGCCA GCGCCCTAGC GCCCGCTCCT 
     8041 TTCGCTTTCT TCCCTTCCTT TCTCGCCACG TTCGCCGGCT TTCCCCGTCA AGCTCTAAAT 
     8101 CGGGGGCTCC CTTTAGGGTT CCGATTTAGT GCTTTACGGC ACCTCGACCC CAAAAAACTT 
     8161 GATTAGGGTG ATGGTTCACG TAGTGGGCCA TCGCCCTGAT AGACGGTTTT TCGCCCTTTG 
     8221 ACGTTGGAGT CCACGTTCTT TAATAGTGGA CTCTTGTTCC AAACTGGAAC AACACTCAAC 
     8281 CCTATCTCGG TCTATTCTTT TGATTTATAA GGGATTTTGC CGATTTCGGC CTATTGGTTA 
     8341 AAAAATGAGC TGATTTAACA AAAATTTAAC GCGAATTTT
//