LOCUS       RR212123                9381 bp    DNA     circular  SYN 
DEFINITION  Cps1 (Myc-DDK-tagged ORF) - Rat carbamoyl-phosphate synthetase 1, mitochondrial (Cps1), nuclear gene encoding mitochondrial protein, (10 ug)
ACCESSION   NM_017072
VERSION     NM_017072.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    5538..5629 
                     /label="Myc-DDK"  
     misc_feature    5659..6244 
                     /label="PolyA signal"  
     misc_feature    5716..5735 
                     /label="Restriction Enzyme"  
     misc_feature    6520..7192 
                     /label="ColE1"  
     misc_feature    7622..8416 
                     /label="Kan/Neo"  
     misc_feature    8451..8753 
                     /label="SV40 ori"  
     misc_feature    8839..9316 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    5520..5526 
                     /label="Restriction Enzyme"  
     misc_feature    1020..5520 
                     /label="Cps1"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GACGAGGATT TTGACAGCAT GCAAAGTGGT GAAGACACTG AAGAGTGGCT 
     1081 TTGGTTTGGC CAATGTGACT TCGAAGCGAC AGTGGGACTT CTCTAGACCT GGCATCAGGC 
     1141 TCCTTTCTGT GAAGGCACAG ACAGCACACA TTGTTCTGGA AGATGGAACT AAGATGAAGG 
     1201 GCTACTCCTT TGGCCATCCC TCCTCGGTTG CTGGCGAAGT GGTTTTTAAT ACTGGCTTAG 
     1261 GAGGGTACTC GGAAGCACTT ACTGATCCTG CCTACAAGGG GCAGATCCTC ACCATGGCCA 
     1321 ACCCTATCAT TGGGAATGGT GGGGCCCCTG ATACAACGGC ACGTGATGAA CTGGGACTGA 
     1381 ATAAGTACAT GGAGTCTGAT GGAATCAAGG TGGCGGGTCT GCTGGTGCTG AATTACAGTC 
     1441 ATGACTACAA CCACTGGCTG GCCACCAAGA GTCTGGGTCA GTGGCTGCAG GAGGAGAAGG 
     1501 TCCCTGCAAT TTATGGAGTG GATACAAGAA TGCTGACTAA AATAATTCGG GATAAGGGTA 
     1561 CCATGCTTGG GAAGATTGAG TTTGAGGGCC AGTCTGTGGA CTTTGTGGAT CCTAATAAGC 
     1621 AGAATTTGAT TGCCGAGGTT TCAACCAAGG ATGTCAAGGT GTTTGGCAAA GGAAACCCCA 
     1681 CGAAAGTGGT AGCCGTGGAC TGTGGGATAA AAAACAATGT CATCCGCCTG CTAGTTAAGC 
     1741 GAGGAGCGGA AGTGCATTTG GTCCCCTGGA ATCATGACTT CACCCAGATG GACTATGACG 
     1801 GACTTCTGAT CGCTGGAGGA CCTGGGAACC CAGCTCTGGC ACAGCCACTA ATTCAGAACG 
     1861 TGAAGAAGAT TTTGGAGAGT GACCGCAAAG AGCCGTTGTT TGGAATCAGT ACAGGAAACA 
     1921 TAATAACAGG ATTGGCTGCT GGCGCCAAAT CCTACAAGAT GTCCATGGCC AACAGAGGAC 
     1981 AGAACCAACC TGTTTTGAAT ATCACAAACA GACAGGCTTT CATAACTGCT CAGAATCATG 
     2041 GCTATGCTCT GGACAACACC CTCCCTGCTG GCTGGAAACC ACTGTTTGTC AATGTCAATG 
     2101 ACCAAACAAA TGAGGGGATT ATGCATGAGA GCAAACCCTT CTTCGCTGTG CAGTTCCACC 
     2161 CAGAGGTCAG CCCGGGGCCA ACAGACACTG AGTACCTATT TGATTCCTTC TTCTCGCTGA 
     2221 TAAAGAAGGG CAAAGGCACC ACCATTACCT CAGTTCTGCC CAAGCCAGCA TTGGTGGCAT 
     2281 CTCGAGTCGA GGTTTCCAAG GTCCTTATCC TAGGATCAGG AGGCCTGTCC ATTGGTCAGG 
     2341 CAGGTGAATT TGATTACTCC GGATCTCAGG CTGTAAAAGC CATGAAGGAA GAAAACGTCA 
     2401 AAACAGTCCT GATGAACCCG AACATTGCAT CCGTGCAGAC CAACGAGGTG GGATTGAAGC 
     2461 AGGCAGATGC AGTCTACTTT CTCCCTATCA CCCCCCAGTT TGTCACAGAA GTTATCAAGG 
     2521 CTGAACGGCC CGATGGGTTA ATTCTGGGCA TGGGTGGCCA GACAGCTCTG AACTGTGGAG 
     2581 TGGAACTATT CAAGAGGGGT GTGCTCAAGG AATATGGTGT GAAGGTCTTG GGAACATCGG 
     2641 TCGAGTCCAT TATGGCCACA GAAGACAGGC AGCTTTTCTC AGACAAGCTG AATGAGATCA 
     2701 ACGAGAAGAT TGCTCCTAGC TTTGCAGTGG AATCAATGGA GGATGCCTTG AAGGCAGCAG 
     2761 ACACCATTGG CTACCCTGTG ATGATTCGGT CTGCGTATGC TCTGGGTGGG TTAGGCTCCG 
     2821 GCATCTGTCC CAACAAGGAG ACCTTAATGG ATCTTGGCAC AAAGGCATTT GCTATGACCA 
     2881 ACCAGATCCT GGTGGAGCGG TCAGTGACAG GTTGGAAAGA AATAGAATAT GAAGTTGTCC 
     2941 GAGATGCGGA TGATAACTGT GTCACAGTCT GTAACATGGA GAATGTTGAC GCCATGGGTG 
     3001 TTCACACAGG TGATTCAGTT GTTGTGGCCC CGGCCCAGAC CCTCTCCAAT GCAGAGTTCC 
     3061 AGATGTTGAG ACGCACTTCA ATCAATGTTG TTCGCCACTT GGGCATTGTG GGTGAATGCA 
     3121 ACATTCAGTT TGCTCTTCAC CCCACTTCCA TGGAATACTG TATCATTGAA GTGAATGCCA 
     3181 GGCTCTCCAG GAGTTCTGCC CTGGCCTCCA AGGCCACTGG CTACCCACTG GCGTTCATCG 
     3241 CAGCAAAGAT CGCTCTAGGA ATCCCACTTC CAGAAATCAA GAATGTTGTG TCTGGGAAGA 
     3301 CCTCAGCCTG CTTCGAACCT AGCCTGGATT ACATGGTGAC CAAGATTCCT CGCTGGGACC 
     3361 TTGACCGTTT TCATGGAACA TCCAGCCGAA TTGGTAGCTC AATGAAAAGT GTAGGCGAGG 
     3421 TCATGGCCAT TGGTCGCACC TTTGAGGAAA GTTTCCAGAA GGCTCTGAGG ATGTGCCATC 
     3481 CATCTGTGGA TGGGTTCACT CCCCGTCTCC CAATGAATAA GGAATGGCCA GCAAACCTGG 
     3541 ATCTGAGGAA GGAGCTGTCT GAACCCTCCA GCACCCGCAT CTATGCCATT GCTAAGGCCT 
     3601 TGGAAAACAA CATGTCCCTT GACGAAATCG TGAAGCTCAC ATCCATTGAC AAGTGGTTTT 
     3661 TGTATAAGAT GCGTGACATT TTAAACATGG ATAAGACACT GAAAGGGCTT AACAGTGAGT 
     3721 CTGTTACAGA AGAAACTCTG AGACAGGCCA AAGAGATTGG GTTCTCAGAC AAGCAGATTT 
     3781 CAAAATGTTT AGGACTGACC GAGGCTCAGA CAAGAGAGCT GAGATTGAAG AAAAACATCC 
     3841 ACCCTTGGGT TAAACAGATT GATACATTGG CTGCAGAATA CCCATCAGTG ACAAATTACC 
     3901 TGTATGTTAC CTACAATGGC CAGGAGCACG ACATCAAATT TGATGAACAT GGAATAATGG 
     3961 TGCTGGGCTG TGGCCCATAC CACATTGGCA GCAGTGTGGA ATTTGATTGG TGTGCTGTCT 
     4021 CCAGTATCCG CACACTGCGC CAACTTGGCA AGAAGACGGT GGTGGTGAAT TGTAACCCGG 
     4081 AGACTGTGAG CACTGACTTT GATGAGTGTG ACAAACTCTA CTTTGAAGAG CTGTCTTTGG 
     4141 AGAGAATCCT AGATATCTAC CACCAGGAGG CATGTAATGG CTGCATCATA TCAGTCGGGG 
     4201 GCCAGATTCC AAACAACTTG GCGGTTCCGC TATACAAGAA CGGTGTCAAG ATCATGGGTA 
     4261 CCAGTCCTCT GCAGATCGAT AGGGCTGAGG ATCGCTCCAT CTTCTCGGCT GTCTTAGATG 
     4321 AGCTGAAGGT GGCCCAGGCT CCTTGGAAAG CTGTTAACAC TTTGAACGAG GCGCTGGAAT 
     4381 TTGCAAACTC TGTGGGCTAT CCCTGCTTAC TGAGACCTTC CTATGTTTTG AGTGGGTCTG 
     4441 CCATGAACGT GGTATTCTCT GAGGATGAGA TGAAAAGGTT CCTTGAGGAG GCCACTCGAG 
     4501 TCTCTCAGGA ACACCCAGTG GTGCTGACCA AGTTTATTGA GGGGGCTCGG GAAGTGGAGA 
     4561 TGGACGCTGT TGGCAAAGAA GGACGGGTCA TCTCCCATGC CATCTCTGAA CATGTTGAAG 
     4621 ATGCAGGTGT CCACTCAGGG GATGCCACAC TGATGCTACC TACGCAGACC ATCAGCCAAG 
     4681 GAGCCATTGA AAAGGTGAAG GATGCCACAC GAAAGATTGC AAAGGCTTTT GCCATCTCTG 
     4741 GGCCATTCAA TGTTCAGTTT CTTGTCAAAG GAAATGATGT CTTGGTGATT GAGTGCAATC 
     4801 TGAGAGCCTC TCGATCCTTC CCCTTTGTCT CCAAGACTCT TGGGGTGGAC TTCATTGATG 
     4861 TGGCCACCAA GGTGATGATC GGAGAGAGTG TTGATGAGAA GCATCTACCC ACACTGGAAC 
     4921 AACCCATCAT CCCCTCTGAC TATGTTGCCA TTAAGGCTCC CATGTTTTCC TGGCCCCGAC 
     4981 TGAGGGATGC TGATCCTATT CTGAGATGTG AGATGGCTTC TACTGGAGAG GTGGCGTGTT 
     5041 TTGGTGAGGG CATTCATACA GCCTTCCTAA AGGCAATGCT GTCCACAGGG TTTAAGATAC 
     5101 CTCAGAAGGG CATTCTGATT GGCATCCAGC AATCATTCCG TCCAAGATTC CTTGGTGTTG 
     5161 CTGAGCAGTT ACACAATGAA GGTTTCAAGC TTTTTGCCAC AGAAGCCACA TCAGACTGGC 
     5221 TCAACGCCAA CAATGTTCCT GCCACCCCAG TGGCTTGGCC ATCTCAGGAA GGACAGAATC 
     5281 CCAGCCTCTC TTCCATCAGA AAGTTGATAA GAGACGGAAG CATTGACCTA GTGATTAACC 
     5341 TCCCCAATAA CAACACCAAA TTTGTCCATG ATAATTATGT GATTCGGAGG ACAGCTGTGG 
     5401 ACAGTGGAAT TGCTCTGCTC ACCAATTTCC AGGTGACCAA ACTTTTTGCT GAGGCTGTGC 
     5461 AGAAAGCTCG TACTGTGGAC TCCAAGAGTC TGTTCCACTA CAGGCAGTAC AGCGCTGGAA 
     5521 AGGCAGCAAC GCGTACGCGG CCGCTCGAGC AGAAACTCAT CTCAGAAGAG GATCTGGCAG 
     5581 CAAATGATAT CCTGGATTAC AAGGATGACG ACGATAAGGT TTAAACGGCC GGCCGCGGTC 
     5641 ATAGCTGTTT CCTGAACAGA TCCCGGGTGG CATCCCTGTG ACCCCTCCCC AGTGCCTCTC 
     5701 CTGGCCCTGG AAGTTGCCAC TCCAGTGCCC ACCAGCCTTG TCCTAATAAA ATTAAGTTGC 
     5761 ATCATTTTGT CTGACTAGGT GTCCTTCTAT AATATTATGG GGTGGAGGGG GGTGGTATGG 
     5821 AGCAAGGGGC AAGTTGGGAA GACAACCTGT AGGGCCTGCG GGGTCTATTG GGAACCAAGC 
     5881 TGGAGTGCAG TGGCACAATC TTGGCTCACT GCAATCTCCG CCTCCTGGGT TCAAGCGATT 
     5941 CTCCTGCCTC AGCCTCCCGA GTTGTTGGGA TTCCAGGCAT GCATGACCAG GCTCAGCTAA 
     6001 TTTTTGTTTT TTTGGTAGAG ACGGGGTTTC ACCATATTGG CCAGGCTGGT CTCCAACTCC 
     6061 TAATCTCAGG TGATCTACCC ACCTTGGCCT CCCAAATTGC TGGGATTACA GGCGTGAACC 
     6121 ACTGCTCCCT TCCCTGTCCT TCTGATTTTA AAATAACTAT ACCAGCAGGA GGACGTCCAG 
     6181 ACACAGCATA GGCTACCTGG CCATGCCCAA CCGGTGGGAC ATTTGAGTTG CTTGCTTGGC 
     6241 ACTGTCCTCT CATGCGTTGG GTCCACTCAG TAGATGCCTG TTGAATTGGG TACGCGGCCA 
     6301 GCGGCGAGCG GTATCAGCTC ACTCAAAGGC GGTAATACGG TTATCCACAG AATCAGGGGA 
     6361 TAACGCAGGA AAGAACATGT GAGCAAAAGG CCAGCAAAAG GCCAGGAACC GTAAAAAGGC 
     6421 CGCGTTGCTG GCGTTTTTCC ATAGGCTCCG CCCCCCTGAC GAGCATCACA AAAATCGACG 
     6481 CTCAAGTCAG AGGTGGCGAA ACCCGACAGG ACTATAAAGA TACCAGGCGT TTCCCCCTGG 
     6541 AAGCTCCCTC GTGCGCTCTC CTGTTCCGAC CCTGCCGCTT ACCGGATACC TGTCCGCCTT 
     6601 TCTCCCTTCG GGAAGCGTGG CGCTTTCTCA TAGCTCACGC TGTAGGTATC TCAGTTCGGT 
     6661 GTAGGTCGTT CGCTCCAAGC TGGGCTGTGT GCACGAACCC CCCGTTCAGC CCGACCGCTG 
     6721 CGCCTTATCC GGTAACTATC GTCTTGAGTC CAACCCGGTA AGACACGACT TATCGCCACT 
     6781 GGCAGCAGCC ACTGGTAACA GGATTAGCAG AGCGAGGTAT GTAGGCGGTG CTACAGAGTT 
     6841 CTTGAAGTGG TGGCCTAACT ACGGCTACAC TAGAAGAACA GTATTTGGTA TCTGCGCTCT 
     6901 GCTGAAGCCA GTTACCTTCG GAAAAAGAGT TGGTAGCTCT TGATCCGGCA AACAAACCAC 
     6961 CGCTGGTAGC GGTGGTTTTT TTGTTTGCAA GCAGCAGATT ACGCGCAGAA AAAAAGGATC 
     7021 TCAAGAAGAT CCTTTGATCT TTTCTACGGG GTCTGACGCT CAGTGGAACG AAAACTCACG 
     7081 TTAAGGGATT TTGGTCATGA GATTATCAAA AAGGATCTTC ACCTAGATCC TTTTAAATTA 
     7141 AAAATGAAGT TTTAAATCAA TCTAAAGTAT ATATGAGTAA CCTGAGGCTA TGGCAGGGCC 
     7201 TGCCGCCCCG ACGTTGGCTG CGAGCCCTGG GCCTTCACCC GAACTTGGGG GGTGGGGTGG 
     7261 GGAAAAGGAA GAAACGCGGG CGTATTGGCC CCAATGGGGT CTCGGTGGGG TATCGACAGA 
     7321 GTGCCAGCCC TGGGACCGAA CCCCGCGTTT ATGAACAAAC GACCCAACAC CGTGCGTTTT 
     7381 ATTCTGTCTT TTTATTGCCG TCATAGCGCG GGTTCCTTCC GGTATTGTCT CCTTCCGTGT 
     7441 TTCAGTTAGC CTCCCCCTAG GGTGGGCGAA GAACTCCAGC ATGAGATCCC CGCGCTGGAG 
     7501 GATCATCCAG CCGGCGTCCC GGAAAACGAT TCCGAAGCCC AACCTTTCAT AGAAGGCGGC 
     7561 GGTGGAATCG AAATCTCGTG ATGGCAGGTT GGGCGTCGCT TGGTCGGTCA TTTCGAACCC 
     7621 CAGAGTCCCG CTCAGAAGAA CTCGTCAAGA AGGCGATAGA AGGCGATGCG CTGCGAATCG 
     7681 GGAGCGGCGA TACCGTAAAG CACGAGGAAG CGGTCAGCCC ATTCGCCGCC AAGCTCTTCA 
     7741 GCAATATCAC GGGTAGCCAA CGCTATGTCC TGATAGCGAT CCGCCACACC CAGCCGGCCA 
     7801 CAGTCGATGA ATCCAGAAAA GCGGCCATTT TCCACCATGA TATTCGGCAA GCAGGCATCG 
     7861 CCATGGGTCA CGACGAGATC CTCGCCGTCG GGCATGCTCG CCTTGAGCCT GGCGAACAGT 
     7921 TCGGCTGGCG CGAGCCCCTG ATGCTCTTCG TCCAGATCAT CCTGATCGAC AAGACCGGCT 
     7981 TCCATCCGAG TACGTGCTCG CTCGATGCGA TGTTTCGCTT GGTGGTCGAA TGGGCAGGTA 
     8041 GCCGGATCAA GCGTATGCAG CCGCCGCATT GCATCAGCCA TGATGGATAC TTTCTCGGCA 
     8101 GGAGCAAGGT GAGATGACAG GAGATCCTGC CCCGGCACTT CGCCCAATAG CAGCCAGTCC 
     8161 CTTCCCGCTT CAGTGACAAC GTCGAGCACA GCTGCGCAAG GAACGCCCGT CGTGGCCAGC 
     8221 CACGATAGCC GCGCTGCCTC GTCTTGCAGT TCATTCAGGG CACCGGACAG GTCGGTCTTG 
     8281 ACAAAAAGAA CCGGGCGCCC CTGCGCTGAC AGCCGGAACA CGGCGGCATC AGAGCAGCCG 
     8341 ATTGTCTGTT GTGCCCAGTC ATAGCCGAAT AGCCTCTCCA CCCAAGCGGC CGGAGAACCT 
     8401 GCGTGCAATC CATCTTGTTC AATCATGCGA AACGATCCTC ATCCTGTCTC TTGATCGATC 
     8461 TTTGCAAAAG CCTAGGCCTC CAAAAAAGCC TCCTCACTAC TTCTGGAATA GCTCAGAGGC 
     8521 CGAGGCGGCC TCGGCCTCTG CATAAATAAA AAAAATTAGT CAGCCATGGG GCGGAGAATG 
     8581 GGCGGAACTG GGCGGAGTTA GGGGCGGGAT GGGCGGAGTT AGGGGCGGGA CTATGGTTGC 
     8641 TGACTAATTG AGATGCATGC TTTGCATACT TCTGCCTGCT GGGGAGCCTG GGGACTTTCC 
     8701 ACACCTGGTT GCTGACTAAT TGAGATGCAT GCTTTGCATA CTTCTGCCTG CTGGGGAGCC 
     8761 TGGGGACTTT CCACACCCTA ACTGACACAC ATTCCACAGC TGGTTCTTTC CGCCTCAGGA 
     8821 CTCTTCCTTT TTCAATATTA TTGAAGCATT TATCAGGGTT ATTGTCTCAT GAGCGGATAC 
     8881 ATATTTGAAT GTATTTAGAA AAATAAACAA ATAGGGGTTC CGCGCACATT TCCCCGAAAA 
     8941 GTGCCACCTG ACGCGCCCTG TAGCGGCGCA TTAAGCGCGG CGGGTGTGGT GGTTACGCGC 
     9001 AGCGTGACCG CTACACTTGC CAGCGCCCTA GCGCCCGCTC CTTTCGCTTT CTTCCCTTCC 
     9061 TTTCTCGCCA CGTTCGCCGG CTTTCCCCGT CAAGCTCTAA ATCGGGGGCT CCCTTTAGGG 
     9121 TTCCGATTTA GTGCTTTACG GCACCTCGAC CCCAAAAAAC TTGATTAGGG TGATGGTTCA 
     9181 CGTAGTGGGC CATCGCCCTG ATAGACGGTT TTTCGCCCTT TGACGTTGGA GTCCACGTTC 
     9241 TTTAATAGTG GACTCTTGTT CCAAACTGGA ACAACACTCA ACCCTATCTC GGTCTATTCT 
     9301 TTTGATTTAT AAGGGATTTT GCCGATTTCG GCCTATTGGT TAAAAAATGA GCTGATTTAA 
     9361 CAAAAATTTA ACGCGAATTT T
//