LOCUS       RR212118                6997 bp    DNA     circular  SYN 
DEFINITION  Pcsk9 (Myc-DDK-tagged ORF) - Rat proprotein convertase subtilisin/kexin type 9 (Pcsk9), (10 ug)
ACCESSION   NM_199253
VERSION     NM_199253.2
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3154..3245 
                     /label="Myc-DDK"  
     misc_feature    3275..3860 
                     /label="PolyA signal"  
     misc_feature    3332..3351 
                     /label="Restriction Enzyme"  
     misc_feature    4136..4808 
                     /label="ColE1"  
     misc_feature    5238..6032 
                     /label="Kan/Neo"  
     misc_feature    6067..6369 
                     /label="SV40 ori"  
     misc_feature    6455..6932 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3136..3142 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3136 
                     /label="Pcsk9"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGCATCCGC TGCTCTACAT GGTTGCGGTG GCCGCTGTCG CCGCAGCTGC 
     1081 TGTTGTTGCT GCTACTGTGC CCCACAGGCT CCCGTGCCCA GGACGAGGAC GGAGACTACG 
     1141 AAGAGCTGAT GCTCGCCCTC CCGTCCCAGG AGGATAGCCT GGTTGATGAG GCCTCACACG 
     1201 TGGCCACCGC CACCTTCCGC CGTTGCTCCA AGGAGGCCTG GAGGCTGCCA GGAACCTACG 
     1261 TGGTGGTGCT GATGGAGGAG ACCCAGCGGC TGCAGGTTGA ACAAACTGCC CATCGCCTGC 
     1321 AGACCTGGGC GGCCCGCCGG GGCTATGTCA TCAAGGTTCT GCATGTCTTT TATGACCTCT 
     1381 TCCCTGGCTT CTTGGTGAAG ATGAGCAGTG ACCTGTTGGG CCTGGCCCTG AAGTTGCCCC 
     1441 ATGTGGAGTA CATCGAGGAA GACTCATTAG TCTTCGCCCA GAGCATCCCA TGGAACCTGG 
     1501 AGCGGATTAT CCCAGCGTGG CAGCAGACAG AGGAAGATAG CTCCCCTGAC GGAAGTAGCC 
     1561 AGGTGGAGGT GTATCTCTTA GATACCAGCA TCCAGAGTGG CCACCGGGAG ATCGAGGGCA 
     1621 GAGTTACCAT CACTGACTTC AACAGTGTGC CTGAGGAGGA CGGGACACGT TTCCACAGAC 
     1681 AGGCGAGCAA GTGTGACAGC CATGGCACCC ACCTAGCAGG TGTGGTCAGC GGCCGGGATG 
     1741 CTGGTGTGGC CAAGGGCACC AGTCTGCACA GTCTGCGTGT GCTCAACTGT CAAGGGAAGG 
     1801 GCACAGTCAG CGGCACCCTC ATAGGCCTGG AGTTTATTCG GAAGAGCCAG CTAATCCAGC 
     1861 CTTCGGGGCC ACTCGTGGTG CTGCTGCCCC TGGCGGGTGG GTATAGCCGG ATCCTTAACA 
     1921 CTGCCTGCCA GCGCCTGGCA AGGACTGGGG TAGTGCTGGT GGCAGCAGCT GGGAATTTCC 
     1981 GAGATGATGC CTGCCTCTAC TCCCCAGCCT CTGCTCCAGA GGTCATTACA GTTGGGGCCA 
     2041 CTAATGCCCA GGACCAGCCA GTCACCCTGG GGACTTTGGG GACAAACTTT GGACGCTGTG 
     2101 TGGATCTCTT TGCCCCCGGG AAGGACATCA TCGGAGCCTC CAGTGACTGT AGCACGTGCT 
     2161 ACATGTCACA GAGTGGGACG TCACAAGCTG CTGCCCACGT GGCTGGCATT GTGGCTATGA 
     2221 TGCTGAACCG GGATCCAGCA CTTACCCTGG CTGAGCTGCG GCAGAGGTTG ATCCTCTTCT 
     2281 CTACCAAAGA TGTCATCAAC ATGGCCTGGT TCCCTGAGGA CCAGCGGGTG CTGACCCCCA 
     2341 ACCGGGTGGC CACACTGCCC CCCAGCACCC AGGAGACAGG CGGGCAGCTG CTCTGCCGGA 
     2401 CAGTGTGGTC CGCCCACTCA GGGCCCACCC GTACAGCAAC AGCCACAGCC CGCTGTGCCC 
     2461 CTGAAGAGGA ACTGCTGAGC TGCTCCAGCT TCTCCAGGAG CGGGAGGCGA CGGGGTGATC 
     2521 GAATTGAGGC CATAGGGGGC CAGCAGGTCT GCAAGGCCCT CAATGCATTT GGGGGTGAGG 
     2581 GTGTCTATGC TGTCGCAAGG TGCTGCCTGC TTCCCCGTGT CAACTGCAGC ATCCACAACA 
     2641 CTCCTGCAGC CAGAGCTGGT CCGCAGACCC CCGTCCACTG CCACCAGAAG GACCATGTTC 
     2701 TCACAGGCTG CAGCTTCCAC TGGGAAGTGG AAAACCTTAG AGCCCAGCAG CAGCCTCTGC 
     2761 TGAGGTCCAG ACATCAACCT GGCCAATGCG TTGGCCACCA GGAGGCCAGT GTCCACGCTT 
     2821 CCTGCTGCCA TGCTCCAGGT CTGGAATGCA AAATCAAGGA GCATGGCATC GCAGGTCCTG 
     2881 CAGAGCAGGT CACCGTGGCC TGTGAGGCAG GATGGACCCT GACTGGATGC AACGTTCTCC 
     2941 CTGGGGCATC CCTCCCTCTG GGGGCCTACA GTGTGGACAA CGTGTGTGTG GCACGAATCC 
     3001 GTGATGCTGG TAGAGCGGAC AGGACCAGTG AAGAAGCCAC GGTAGCTGCT GCCATCTGCT 
     3061 GCCGGAGCCG GCCTTCGGCA AAGGCCTCCT GGGTTCACCA GCTGCCGGAG CCGGCCTTCG 
     3121 GCAAAGGCCT CCTGGGTTCA CCAGACGCGT ACGCGGCCGC TCGAGCAGAA ACTCATCTCA 
     3181 GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG ATGACGACGA TAAGGTTTAA 
     3241 ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC GGGTGGCATC CCTGTGACCC 
     3301 CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA GTGCCCACCA GCCTTGTCCT 
     3361 AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC TTCTATAATA TTATGGGGTG 
     3421 GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA ACCTGTAGGG CCTGCGGGGT 
     3481 CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG CTCACTGCAA TCTCCGCCTC 
     3541 CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG TTGGGATTCC AGGCATGCAT 
     3601 GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG GGTTTCACCA TATTGGCCAG 
     3661 GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT TGGCCTCCCA AATTGCTGGG 
     3721 ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG ATTTTAAAAT AACTATACCA 
     3781 GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT GCCCAACCGG TGGGACATTT 
     3841 GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC ACTCAGTAGA TGCCTGTTGA 
     3901 ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC AAAGGCGGTA ATACGGTTAT 
     3961 CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC AAAAGGCCAG CAAAAGGCCA 
     4021 GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG GCTCCGCCCC CCTGACGAGC 
     4081 ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC GACAGGACTA TAAAGATACC 
     4141 AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT TCCGACCCTG CCGCTTACCG 
     4201 GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT TTCTCATAGC TCACGCTGTA 
     4261 GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG CTGTGTGCAC GAACCCCCCG 
     4321 TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT TGAGTCCAAC CCGGTAAGAC 
     4381 ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT TAGCAGAGCG AGGTATGTAG 
     4441 GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG CTACACTAGA AGAACAGTAT 
     4501 TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA AAGAGTTGGT AGCTCTTGAT 
     4561 CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT TTGCAAGCAG CAGATTACGC 
     4621 GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC TACGGGGTCT GACGCTCAGT 
     4681 GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT ATCAAAAAGG ATCTTCACCT 
     4741 AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA AAGTATATAT GAGTAACCTG 
     4801 AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG CCCTGGGCCT TCACCCGAAC 
     4861 TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA TTGGCCCCAA TGGGGTCTCG 
     4921 GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC GCGTTTATGA ACAAACGACC 
     4981 CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT AGCGCGGGTT CCTTCCGGTA 
     5041 TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG GGCGAAGAAC TCCAGCATGA 
     5101 GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA AACGATTCCG AAGCCCAACC 
     5161 TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG CAGGTTGGGC GTCGCTTGGT 
     5221 CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG TCAAGAAGGC GATAGAAGGC 
     5281 GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG AGGAAGCGGT CAGCCCATTC 
     5341 GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT ATGTCCTGAT AGCGATCCGC 
     5401 CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG CCATTTTCCA CCATGATATT 
     5461 CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG CCGTCGGGCA TGCTCGCCTT 
     5521 GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC TCTTCGTCCA GATCATCCTG 
     5581 ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG ATGCGATGTT TCGCTTGGTG 
     5641 GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC CGCATTGCAT CAGCCATGAT 
     5701 GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA TCCTGCCCCG GCACTTCGCC 
     5761 CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG AGCACAGCTG CGCAAGGAAC 
     5821 GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT TGCAGTTCAT TCAGGGCACC 
     5881 GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC GCTGACAGCC GGAACACGGC 
     5941 GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG CCGAATAGCC TCTCCACCCA 
     6001 AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC ATGCGAAACG ATCCTCATCC 
     6061 TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA AAAGCCTCCT CACTACTTCT 
     6121 GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA AATAAAAAAA ATTAGTCAGC 
     6181 CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG CGGGATGGGC GGAGTTAGGG 
     6241 GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG CATACTTCTG CCTGCTGGGG 
     6301 AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG ATGCATGCTT TGCATACTTC 
     6361 TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG ACACACATTC CACAGCTGGT 
     6421 TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA AGCATTTATC AGGGTTATTG 
     6481 TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT AAACAAATAG GGGTTCCGCG 
     6541 CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC GGCGCATTAA GCGCGGCGGG 
     6601 TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC GCCCTAGCGC CCGCTCCTTT 
     6661 CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT CCCCGTCAAG CTCTAAATCG 
     6721 GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC CTCGACCCCA AAAAACTTGA 
     6781 TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG ACGGTTTTTC GCCCTTTGAC 
     6841 GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA ACTGGAACAA CACTCAACCC 
     6901 TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG ATTTCGGCCT ATTGGTTAAA 
     6961 AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//