LOCUS       RR212087                7737 bp    DNA     circular  SYN 
DEFINITION  Usp15 (Myc-DDK-tagged ORF) - Rat ubiquitin specific peptidase 15 (Usp15), (10 ug)
ACCESSION   NM_145184
VERSION     NM_145184.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3894..3985 
                     /label="Myc-DDK"  
     misc_feature    4015..4600 
                     /label="PolyA signal"  
     misc_feature    4072..4091 
                     /label="Restriction Enzyme"  
     misc_feature    4876..5548 
                     /label="ColE1"  
     misc_feature    5978..6772 
                     /label="Kan/Neo"  
     misc_feature    6807..7109 
                     /label="SV40 ori"  
     misc_feature    7195..7672 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3876..3882 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3876 
                     /label="Usp15"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGCGGAAGGC GGAGCGGCGG ACCTGGACAC CCAGCGTTCT GACATCGCGA 
     1081 CGCTGCTCAA AACCTCGCTC CGGAAAGGGG ACACCTGGTA TCTAGTAGAT AGTCGGTGGT 
     1141 TCAAACAGTG GAAAAAATAT GTTGGCTTTG ACAGTTGGGA CAAATACCAG ATGGGAGATC 
     1201 AAAATGTCTA TCCTGGACCC ATCGATAACT CTGGACTTCT CAAAGATGGC GATGCTCAGT 
     1261 CACTTAAGGA ACACCTTATC GACGAGTTGG ATTACATACT GTTGCCGACT GAGGGCTGGA 
     1321 ATAAACTTGT CAGCTGGTAC ACACTGATGG AAGGCCAAGA GCCAATAGCA AGAAAGGTGG 
     1381 TAGAACAGGG CATGTTTGTA AAGCACTGCA AAGTGGAAGT CTACCTCACA GAACTGAAGC 
     1441 TCTGCGAGAA TGGGAACATG AACAATGTTG TAACTCGGAG ATTTAGCAAA GCTGACACAA 
     1501 TAGACACAAT TGAAAAGGAA ATAAGAAAGA TCTTCAATAT TCCAGATGAA AAGGAGGCCA 
     1561 GACTGTGGAA CAAGTATATG AGTAACACAT TTGAGCCACT GAATAAGCCA GACAGCACCA 
     1621 TCCAGGATGC CGGCCTATAC CAAGGACAGG TGTTAGTGAT AGAACAAAAA AATGAAGACG 
     1681 GAACGTGGCC CAGGGGTCCC TCTGCTCCTA ATGTGAAAAA CTCCAATTAT TGTCTCCCGT 
     1741 CATATACTGC TTACAAGAAC TATGATTATT CGGAACCTGG AAGAAACAAT GAACAGCCAG 
     1801 GCCTGTGTGG CCTAAGTAAC TTGGGAAACA CATGTTTCAT GAACTCAGCC ATTCAGTGTT 
     1861 TGAGCAACAC ACCTCCACTT ACTGAATATT TCCTCAATGA TAAGTACCAA GAAGAGCTGA 
     1921 ATTTTGACAA TCCCTTAGGA ATGAGAGGTG AAATAGCTAA ATCATATGCT GAACTCATCA 
     1981 AGCAGATGTG GTCTGGAAAG TTTAGCTATG TCACTCCAAG AGCATTTAAG ACACAAGTAG 
     2041 GACGTTTTGC ACCTCAGTTC TCTGGATATC AACAGCAAGA CTGCCAAGAA CTACTAGCTT 
     2101 TCCTATTAGA TGGATTACAT GAGGATTTGA ATAGAATTAG GAAAAAACCA TATATCCAGT 
     2161 TAAAAGATGC TGATGGGAGG CCAGACAAGG TGGTTGCTGA AGAAGCCTGG GAAAACCATT 
     2221 TAAAAAGAAA TGATTCTATC ATAGTAGATA TATTTCATGG CCTTTTCAAG TCTACGTTAG 
     2281 TGTGTCCTGA GTGTGCTAAG ATCTCAGTGA CCTTCGACCC TTTCTGTTAC TTGACACTTC 
     2341 CATTGCCTAT GAAAAAGGAA CGTAGCTTAG AAGTTTATTT AGTTAGAATG GATCCACTTG 
     2401 CTAAACCTAT GCAATACAAA GTCATTGTGC CCAAAATTGG AAATATACTA GACCTTTGTA 
     2461 CAGCACTGTC TGCTTTGTCG GGAGTACCTG CAGATAAGAT GATAGTCACT GACATATATA 
     2521 ATCATAGATT TCACAGGATA TTTGCAATGG ATGAGAACCT GAGTAGTATT ATGGAGCGGG 
     2581 ATGATATTTA TGTGTTTGAA ATTAACATCA ACAGGACAGA AGACACAGAA CATGTGGTTA 
     2641 TTCCTGTTTG CCTAAGAGAA AAGTTCAGAC ACTCAAGTTA CACTCACCAC ACTGGCTCTT 
     2701 CACTGTTCGG CCAGCCTTTT CTTATGGCCG TGCCACGGAA CAATACTGAA GATAAACTCT 
     2761 ACAATCTCCT GCTCTTGAGA ATGTGCCGGT ATGTCAAGAT GTCTACTGAA ACTGAGGAAA 
     2821 CTGATGGACC CCTGCGTTGC TGTGAGGACC AGAATATTAA TGGGAATGGT CCAAATGGCA 
     2881 TACATGAAGA AGGCTCACCA AGTGAGATGG AGACAGATGA ACCAGATGAT GAGTCCAGCC 
     2941 AGGATCAAGA GCTTCCCTCA GAAAATGAGA ACAGTCAGTC TGAAGATTCA GTCGGAGGAG 
     3001 ATAACGATTC TGAAAATGGA TTGTGTACTG AAGAAACATG CAAAGGTCGA CTGACGGGAC 
     3061 ACAAAAAGCG ATTGTTTACA TTCCAGTTCA ACAACTTAGG CAACACTGAT ATCAACTATA 
     3121 TCAAAGACGA CACCAGGCAT ATAAGATTTG ATGATAGGCA GCTTCGGCTA GATGAAAGAT 
     3181 CTTTTCTCGC CTTGGACTGG GACCCTGACT TGAAGAAGAG ATATTTTGAT GAGAATGCTG 
     3241 CAGAGGATTT TGAAAAACAT GAAAGCGTGG AATATAAACC TCCAAAAAGA CCCTTTGTGA 
     3301 AGTTAAAAGA CTGCATTGAA CTTTTCACAA CAAAAGAGAA GCTAGGTGCT GAAGACCCCT 
     3361 GGTATTGTCC AAATTGTAAA GAGCATCAGC AAGCCACCAA GAAATTAGAT CTGTGGTCCC 
     3421 TGCCTCCCGT TCTTGTGGTG CATCTCAAGA GATTCTCCTA TAGTCGCTAC ATGAGAGACA 
     3481 AGCTAGATAC ATTAGTCGAT TTTCCCATCA GTGACTTGGA TATGTCGGAA TTCCTAATTA 
     3541 ACCCAAATGC AGGCCCTTGC CGCTATAATT TGATTGCTGT TTCCAATCAC TATGGAGGGA 
     3601 TGGGAGGAGG ACATTATACT GCTTTTGCAA AGAACAAAGA TGATGGAAAG TGGTACTATT 
     3661 TTGATGACAG TAGCGTCTCC AGTGCATCTG AAGACCAAAT TGTGTCCAAA GCAGCATACG 
     3721 TGCTCTTCTA CCAGAGACAG GACACTTTCA GTGGAACTGG CTTCTTCCCT CTCGACCGAG 
     3781 AAACTAAAGG TGCTTCAGCT GCCACAGGTG TCCCCCTGGA AAGTGACGAA GACAGCAATG 
     3841 ATAATGACAA TGATCTAGAA AATGAAAACT GTATGCACAC TAATACGCGT ACGCGGCCGC 
     3901 TCGAGCAGAA ACTCATCTCA GAAGAGGATC TGGCAGCAAA TGATATCCTG GATTACAAGG 
     3961 ATGACGACGA TAAGGTTTAA ACGGCCGGCC GCGGTCATAG CTGTTTCCTG AACAGATCCC 
     4021 GGGTGGCATC CCTGTGACCC CTCCCCAGTG CCTCTCCTGG CCCTGGAAGT TGCCACTCCA 
     4081 GTGCCCACCA GCCTTGTCCT AATAAAATTA AGTTGCATCA TTTTGTCTGA CTAGGTGTCC 
     4141 TTCTATAATA TTATGGGGTG GAGGGGGGTG GTATGGAGCA AGGGGCAAGT TGGGAAGACA 
     4201 ACCTGTAGGG CCTGCGGGGT CTATTGGGAA CCAAGCTGGA GTGCAGTGGC ACAATCTTGG 
     4261 CTCACTGCAA TCTCCGCCTC CTGGGTTCAA GCGATTCTCC TGCCTCAGCC TCCCGAGTTG 
     4321 TTGGGATTCC AGGCATGCAT GACCAGGCTC AGCTAATTTT TGTTTTTTTG GTAGAGACGG 
     4381 GGTTTCACCA TATTGGCCAG GCTGGTCTCC AACTCCTAAT CTCAGGTGAT CTACCCACCT 
     4441 TGGCCTCCCA AATTGCTGGG ATTACAGGCG TGAACCACTG CTCCCTTCCC TGTCCTTCTG 
     4501 ATTTTAAAAT AACTATACCA GCAGGAGGAC GTCCAGACAC AGCATAGGCT ACCTGGCCAT 
     4561 GCCCAACCGG TGGGACATTT GAGTTGCTTG CTTGGCACTG TCCTCTCATG CGTTGGGTCC 
     4621 ACTCAGTAGA TGCCTGTTGA ATTGGGTACG CGGCCAGCGG CGAGCGGTAT CAGCTCACTC 
     4681 AAAGGCGGTA ATACGGTTAT CCACAGAATC AGGGGATAAC GCAGGAAAGA ACATGTGAGC 
     4741 AAAAGGCCAG CAAAAGGCCA GGAACCGTAA AAAGGCCGCG TTGCTGGCGT TTTTCCATAG 
     4801 GCTCCGCCCC CCTGACGAGC ATCACAAAAA TCGACGCTCA AGTCAGAGGT GGCGAAACCC 
     4861 GACAGGACTA TAAAGATACC AGGCGTTTCC CCCTGGAAGC TCCCTCGTGC GCTCTCCTGT 
     4921 TCCGACCCTG CCGCTTACCG GATACCTGTC CGCCTTTCTC CCTTCGGGAA GCGTGGCGCT 
     4981 TTCTCATAGC TCACGCTGTA GGTATCTCAG TTCGGTGTAG GTCGTTCGCT CCAAGCTGGG 
     5041 CTGTGTGCAC GAACCCCCCG TTCAGCCCGA CCGCTGCGCC TTATCCGGTA ACTATCGTCT 
     5101 TGAGTCCAAC CCGGTAAGAC ACGACTTATC GCCACTGGCA GCAGCCACTG GTAACAGGAT 
     5161 TAGCAGAGCG AGGTATGTAG GCGGTGCTAC AGAGTTCTTG AAGTGGTGGC CTAACTACGG 
     5221 CTACACTAGA AGAACAGTAT TTGGTATCTG CGCTCTGCTG AAGCCAGTTA CCTTCGGAAA 
     5281 AAGAGTTGGT AGCTCTTGAT CCGGCAAACA AACCACCGCT GGTAGCGGTG GTTTTTTTGT 
     5341 TTGCAAGCAG CAGATTACGC GCAGAAAAAA AGGATCTCAA GAAGATCCTT TGATCTTTTC 
     5401 TACGGGGTCT GACGCTCAGT GGAACGAAAA CTCACGTTAA GGGATTTTGG TCATGAGATT 
     5461 ATCAAAAAGG ATCTTCACCT AGATCCTTTT AAATTAAAAA TGAAGTTTTA AATCAATCTA 
     5521 AAGTATATAT GAGTAACCTG AGGCTATGGC AGGGCCTGCC GCCCCGACGT TGGCTGCGAG 
     5581 CCCTGGGCCT TCACCCGAAC TTGGGGGGTG GGGTGGGGAA AAGGAAGAAA CGCGGGCGTA 
     5641 TTGGCCCCAA TGGGGTCTCG GTGGGGTATC GACAGAGTGC CAGCCCTGGG ACCGAACCCC 
     5701 GCGTTTATGA ACAAACGACC CAACACCGTG CGTTTTATTC TGTCTTTTTA TTGCCGTCAT 
     5761 AGCGCGGGTT CCTTCCGGTA TTGTCTCCTT CCGTGTTTCA GTTAGCCTCC CCCTAGGGTG 
     5821 GGCGAAGAAC TCCAGCATGA GATCCCCGCG CTGGAGGATC ATCCAGCCGG CGTCCCGGAA 
     5881 AACGATTCCG AAGCCCAACC TTTCATAGAA GGCGGCGGTG GAATCGAAAT CTCGTGATGG 
     5941 CAGGTTGGGC GTCGCTTGGT CGGTCATTTC GAACCCCAGA GTCCCGCTCA GAAGAACTCG 
     6001 TCAAGAAGGC GATAGAAGGC GATGCGCTGC GAATCGGGAG CGGCGATACC GTAAAGCACG 
     6061 AGGAAGCGGT CAGCCCATTC GCCGCCAAGC TCTTCAGCAA TATCACGGGT AGCCAACGCT 
     6121 ATGTCCTGAT AGCGATCCGC CACACCCAGC CGGCCACAGT CGATGAATCC AGAAAAGCGG 
     6181 CCATTTTCCA CCATGATATT CGGCAAGCAG GCATCGCCAT GGGTCACGAC GAGATCCTCG 
     6241 CCGTCGGGCA TGCTCGCCTT GAGCCTGGCG AACAGTTCGG CTGGCGCGAG CCCCTGATGC 
     6301 TCTTCGTCCA GATCATCCTG ATCGACAAGA CCGGCTTCCA TCCGAGTACG TGCTCGCTCG 
     6361 ATGCGATGTT TCGCTTGGTG GTCGAATGGG CAGGTAGCCG GATCAAGCGT ATGCAGCCGC 
     6421 CGCATTGCAT CAGCCATGAT GGATACTTTC TCGGCAGGAG CAAGGTGAGA TGACAGGAGA 
     6481 TCCTGCCCCG GCACTTCGCC CAATAGCAGC CAGTCCCTTC CCGCTTCAGT GACAACGTCG 
     6541 AGCACAGCTG CGCAAGGAAC GCCCGTCGTG GCCAGCCACG ATAGCCGCGC TGCCTCGTCT 
     6601 TGCAGTTCAT TCAGGGCACC GGACAGGTCG GTCTTGACAA AAAGAACCGG GCGCCCCTGC 
     6661 GCTGACAGCC GGAACACGGC GGCATCAGAG CAGCCGATTG TCTGTTGTGC CCAGTCATAG 
     6721 CCGAATAGCC TCTCCACCCA AGCGGCCGGA GAACCTGCGT GCAATCCATC TTGTTCAATC 
     6781 ATGCGAAACG ATCCTCATCC TGTCTCTTGA TCGATCTTTG CAAAAGCCTA GGCCTCCAAA 
     6841 AAAGCCTCCT CACTACTTCT GGAATAGCTC AGAGGCCGAG GCGGCCTCGG CCTCTGCATA 
     6901 AATAAAAAAA ATTAGTCAGC CATGGGGCGG AGAATGGGCG GAACTGGGCG GAGTTAGGGG 
     6961 CGGGATGGGC GGAGTTAGGG GCGGGACTAT GGTTGCTGAC TAATTGAGAT GCATGCTTTG 
     7021 CATACTTCTG CCTGCTGGGG AGCCTGGGGA CTTTCCACAC CTGGTTGCTG ACTAATTGAG 
     7081 ATGCATGCTT TGCATACTTC TGCCTGCTGG GGAGCCTGGG GACTTTCCAC ACCCTAACTG 
     7141 ACACACATTC CACAGCTGGT TCTTTCCGCC TCAGGACTCT TCCTTTTTCA ATATTATTGA 
     7201 AGCATTTATC AGGGTTATTG TCTCATGAGC GGATACATAT TTGAATGTAT TTAGAAAAAT 
     7261 AAACAAATAG GGGTTCCGCG CACATTTCCC CGAAAAGTGC CACCTGACGC GCCCTGTAGC 
     7321 GGCGCATTAA GCGCGGCGGG TGTGGTGGTT ACGCGCAGCG TGACCGCTAC ACTTGCCAGC 
     7381 GCCCTAGCGC CCGCTCCTTT CGCTTTCTTC CCTTCCTTTC TCGCCACGTT CGCCGGCTTT 
     7441 CCCCGTCAAG CTCTAAATCG GGGGCTCCCT TTAGGGTTCC GATTTAGTGC TTTACGGCAC 
     7501 CTCGACCCCA AAAAACTTGA TTAGGGTGAT GGTTCACGTA GTGGGCCATC GCCCTGATAG 
     7561 ACGGTTTTTC GCCCTTTGAC GTTGGAGTCC ACGTTCTTTA ATAGTGGACT CTTGTTCCAA 
     7621 ACTGGAACAA CACTCAACCC TATCTCGGTC TATTCTTTTG ATTTATAAGG GATTTTGCCG 
     7681 ATTTCGGCCT ATTGGTTAAA AAATGAGCTG ATTTAACAAA AATTTAACGC GAATTTT
//