LOCUS       RR212086                8526 bp    DNA     circular  SYN 
DEFINITION  Zeb2 (Myc-DDK-tagged ORF) - Rat zinc finger E-box binding homeobox 2 (Zeb2), (10 ug)
ACCESSION   NM_001033701
VERSION     NM_001033701.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    4683..4774 
                     /label="Myc-DDK"  
     misc_feature    4804..5389 
                     /label="PolyA signal"  
     misc_feature    4861..4880 
                     /label="Restriction Enzyme"  
     misc_feature    5665..6337 
                     /label="ColE1"  
     misc_feature    6767..7561 
                     /label="Kan/Neo"  
     misc_feature    7596..7898 
                     /label="SV40 ori"  
     misc_feature    7984..8461 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    4665..4671 
                     /label="Restriction Enzyme"  
     misc_feature    1020..4665 
                     /label="Zeb2"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GAAGCAGCCG ATCATGGCGG ATGGCCCCCG GTGCAAGAGG CGCAAACAAG 
     1081 CCAATCCCAG GAGGAAAAAC GTGGTGAACT ATGACAACGT AGTGGACGCA GGTTCCGAAA 
     1141 CAGACGAGGA GGACAAGCTG CACATTGCTG AGGATGACGG CATTGCAAAC CCTCTGGACC 
     1201 AGGAGACGAG TCCAGCTAGC ATGCCCAACC ACGAGTCCTC CCCACACATG AGCCAAGGCC 
     1261 TGCTACCAAG AGAGGAAGAA GAAGACGAGA TAAGGGAGAG CGTTGTGGAA CACAGCTGGC 
     1321 ACAGCGGTGA GATTCTGCAA GCCTCTGTAG ACGGTCCAGA AGAAATGAAG GAGGATTATG 
     1381 ATGCTCTGGG GCCAGAAGCC ACGATCCAGA CCACAATTAA CAATGGTACA GTTAAGAATG 
     1441 CAAATTGCAC ATCAGACTTT GAGGAATACT TTGCCAAAAG AAAACTGGAG GAAAGAGATG 
     1501 GCCACGCAGT CAGCATTGAA GAGTACCTTC AGCGAAGCGA CACGGCCATT ATTTACCCAG 
     1561 AAGCCCCTGA GGAGCTGTCT CGCCTTGGCA CGCCAGAGGC CAATGGGCAA GAAGAAAATG 
     1621 ACCTGCCACC TGGAACTCCA GATGCTTTTG CCCAACTGCT GACCTGCCCC TACTGCGACC 
     1681 GCGGCTACAA GCGCTTGACA TCGCTGAAGG AGCACATCAA GTACCGCCAC GAGAAGAATG 
     1741 AAGAGAACTT TTCCTGCCCT CTTTGTAGCT ACACGTTCGC CTACCGCACC CAGCTCGAAA 
     1801 GGCATATGGT GACGCACAAG CCAGGGACAG ATCAGCACCA AATGCTAACC CAAGGAGCAG 
     1861 GTAACCGCAA GTTCAAGTGC ACAGAGTGTG GCAAGGCCTT CAAGTACAAG CACCACCTGA 
     1921 AAGAACACCT GAGAATTCAC AGTGGTGAAA AACCTTACGA ATGCCCCAAC TGCAAGAAAC 
     1981 GCTTCTCTCA TTCTGGTTCC TACAGTTCAC ATATCAGCAG CAAGAAATGT ATTGGTTTAA 
     2041 TCTCAGTAAA TGGCCGAATG AGAAACAATA TCAAGACGGG TTCTTCCCCT AATTCTGTTT 
     2101 CTTCTTCTCC TACTAACTCA GCCATTACTC AACTAAGGAA CAAGTTGGAA AATGGAAAAC 
     2161 CACTTAGCAT GTCTGAGCAG ACGGGCTTAC TTAAGATTAA AACAGAACCA CTAGACTTCA 
     2221 ATGACTATAA AGTTCTTATG GCAACACATG GGTTTAGTGG CACTAGTCCC TTTATGAACG 
     2281 GTGGGCTTGG AGCCACCAGC CCTTTAGGTG TGCACCCATC TGCTCAGAGT CCAATGCAGC 
     2341 ACTTAGGTGT AGGGTTAGAA GCCCCTTTAC TTGGATTTCC CACTATGAAT AGTAATCTGA 
     2401 GTGAGGTACA AAAGGTTCTA CAGATTGTGG ATAATACGGT TTCTAGGCAA AAGATGGACT 
     2461 GCAAGACTGA AGACATTTCA AAGTTGAAAG GTTACCACAT GAAGGATCCA TGTTCTCAGC 
     2521 CAGAAGAACA AGGGGTTACT TCTCCTAGTA TTCCAGCCGT CGGTCTTCCA GTAGTGAGTC 
     2581 ATAATGGTGC CACTAAAAGT ATCATTGACT ATACCTTAGA GAAAGTCAAC GAAGCCAAAG 
     2641 CTTGCCTCCA GAGCTTGACT ACTGACTCAA GGAGACAGAT CAGCAATATA AAGAAAGAGA 
     2701 AGCTACGTAC TTTGATAGAT TTGGTCACCG ATGATAAAAT GATTGAGAAC CACAGCATAC 
     2761 CCACTCCGTT TTCATGCCAG TTCTGTAAAG AAAGCTTCCC AGGTCCTATC CCCCTGCATC 
     2821 AGCATGAACG CTACCTGTGT AAAATGAATG AAGAGATCAA GGCGGTCCTG CAACCTCACG 
     2881 AAAACATAGT CCCCAACAAA GCTGGGGTGT TCGTTGACAA TAAAGCCCTC CTCTTGTCAT 
     2941 CTGTACTTTC TGAGAAAGGA CTGACAAGCC CGATCAACCC GTACAAGGAC CACATGTCTG 
     3001 TACTCAAAGC ATACTATGCT ATGAATATGG AACCCAACTC TGATGAACTG CTGAAAATCT 
     3061 CCATTGCTGT GGGCCTTCCT CAGGAGTTTG TGAAGGAATG GTTTGAGCAG AGAAAAGTCT 
     3121 ACCAGTACTC CAGTTCCAGG TCACCATCAC TGGAGAGGAA CTCCAAGCCC TTAGCTCCCA 
     3181 ACAGCAACCC CACCACAAAA GACTCTTTGT TACCCAGGTC TCCTGTAAAA CCTATGGACT 
     3241 CAATCACGTC ACCATCTATA GCAGAACTCC ACAACAGTGT CACGAGTTGT GATCCTCCTC 
     3301 TCAGGCTAAC AAAATCTTCC CACTTCACCA ATACTAAAGC AGTTGATAAA CTGGACCACT 
     3361 CAAGGAGTAA CACTCCTTCT CCTTTAAATC TTTCTTCCAC GTCTTCTAAA AACTCCCACA 
     3421 GTAGCTCGTA CACTCCAAAT AGCTTCTCTT CCGAGGAGCT GCAGGCTGAG CCGTTGGACC 
     3481 TGTCATTACC AAAACAAATG AGAGAGCCCA AAAGTATTAT AGCCACAAAG AACAAGACGA 
     3541 AAGCTACTAG CATAAGTTTA GACCACAACA GTGTTTCTTC ATCCTCTGAA AACTCAGATG 
     3601 AACCTCTGAA TTTGACTTTT ATCAAGAAAG AGTTTTCAAA TTCTAATAAC CTGGACAATA 
     3661 AAAGCACTAA CCCTGTGTTC GGCATGAACC CATTTAGTGC CAAGCCTTTA TACACAGCTC 
     3721 TTCCACCACA GAGCGCATTT CCCCCTGCCA CTTTCATGCC ACCAGTCCAG ACCAGCATCC 
     3781 CCGGGCTACG ACCATACCCA GGGCTGGATC AGATGAGCTT CCTACCACAT ATGGCCTATA 
     3841 CCTACCCAAC GGGAGCAGCT ACCTTTGCTG ATATGCAGCA AAGGAGGAAA TACCAGCGGA 
     3901 AACAAGGATT TCAGGGAGAA TTGCTTGATG GAGCACAAGA CTACATGTCG GGCCTAGATG 
     3961 ACTTGACAGA CTCCGATTCC TGTCTGTCTC GAAAGAAGAT AAAGAAGACA GAAAGTGGCA 
     4021 TGTATGCATG TGACTTATGT GACAAGACAT TCCAGAAAAG CAGTTCCCTT CTGCGACATA 
     4081 AATACGAACA CACAGGAAAG AGACCACACC AGTGTCAGAT TTGTAAGAAA GCGTTCAAAC 
     4141 ACAAACACCA CCTTATCGAG CACTCGAGGC TGCACTCGGG CGAGAAGCCC TATCAGTGTG 
     4201 ACAAATGTGG CAAGCGCTTT TCACACTCGG GCTCCTACTC GCAGCACATG AATCACAGGT 
     4261 ACTCCTACTG CAAGCGGGAG GCGGAGGAGC GGGAAGCGGC CGAGCGCGAG GCACGAGAGA 
     4321 AAGGGCACTT GGAACCCACC GAGCTGCTGA TGAACCGGGC TTACTTGCAG AGCATCACCC 
     4381 CTCAGGGGTA CTCTGACTCG GAGGAGAGGG AGAGCATGCC GAGGGATGGC GAGAGCGAGA 
     4441 AGGAGCACGA GAAGGAGGGC GAGGAGGGCT ATGGGAAGCT GCGGAGAAGG GACGGCGACG 
     4501 AGGAGGAAGA GGAGGAAGAG GAAGAAAGTG AAAATAAAAG TATGGATACG GATCCCGAGA 
     4561 CGATACGGGA TGAGGAGGAG ACTGGGGATC ACTCAATGGA TGACAGTTCA GAGGATGGGA 
     4621 AAATGGAAAC CAAATCAGAC CACGAGGAAG ACAATATGGA AGACGGCATG GAAACGCGTA 
     4681 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     4741 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     4801 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     4861 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     4921 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4981 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     5041 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     5101 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     5161 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     5221 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     5281 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     5341 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     5401 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     5461 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     5521 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     5581 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     5641 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     5701 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     5761 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     5821 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     5881 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     5941 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     6001 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     6061 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     6121 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     6181 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     6241 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     6301 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     6361 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     6421 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     6481 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     6541 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     6601 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     6661 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     6721 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     6781 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     6841 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     6901 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6961 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     7021 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     7081 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     7141 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     7201 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     7261 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     7321 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     7381 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     7441 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     7501 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     7561 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     7621 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     7681 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     7741 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     7801 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     7861 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     7921 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7981 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     8041 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     8101 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     8161 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     8221 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     8281 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     8341 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     8401 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     8461 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     8521 AATTTT
//