LOCUS       RR212027                7566 bp    DNA     circular  SYN 
DEFINITION  Incenp (Myc-DDK-tagged ORF) - Rat inner centromere protein (Incenp), (10 ug)
ACCESSION   NM_001106335
VERSION     NM_001106335.1
SOURCE      synthetic construct 
FEATURES    Location/Qualifiers 
     misc_feature    201..924 
                     /label="CMV promoter"  
     misc_feature    839..856 
                     /label="Restriction Enzyme"  
     misc_feature    953..971 
                     /label="T7 promoter"  
     misc_feature    3723..3814 
                     /label="Myc-DDK"  
     misc_feature    3844..4429 
                     /label="PolyA signal"  
     misc_feature    3901..3920 
                     /label="Restriction Enzyme"  
     misc_feature    4705..5377 
                     /label="ColE1"  
     misc_feature    5807..6601 
                     /label="Kan/Neo"  
     misc_feature    6636..6938 
                     /label="SV40 ori"  
     misc_feature    7024..7501 
                     /label="f1 ori"  
     misc_feature    1020..1028 
                     /label="Restriction Enzyme"  
     misc_feature    3705..3711 
                     /label="Restriction Enzyme"  
     misc_feature    1020..3705 
                     /label="Incenp"  
ORIGIN                      
        1 AACAAAATAT TAACGCTTAC AATTTCCATT CGCCATTCAG GCTGCGCAAC TGTTGGGAAG 
       61 GGCGATCGGT GCGGGCCTCT TCGCTATTAC GCCAGCTGGC GAAAGGGGGA TGTGCTGCAA 
      121 GGCGATTAAG TTGGGTAACG CCAGGGTTTT CCCAGTCACG ACGTTGTAAA ACGACGGCCA 
      181 GTGCCAAGCT GATCTATACA TTGAATCAAT ATTGGCAATT AGCCATATTA GTCATTGGTT 
      241 ATATAGCATA AATCAATATT GGCTATTGGC CATTGCATAC GTTGTATCTA TATCATAATA 
      301 TGTACATTTA TATTGGCTCA TGTCCAATAT GACCGCCATG TTGACATTGA TTATTGACTA 
      361 GTTATTAATA GTAATCAATT ACGGGGTCAT TAGTTCATAG CCCATATATG GAGTTCCGCG 
      421 TTACATAACT TACGGTAAAT GGCCCGCCTG GCTGACCGCC CAACGACCCC CGCCCATTGA 
      481 CGTCAATAAT GACGTATGTT CCCATAGTAA CGCCAATAGG GACTTTCCAT TGACGTCAAT 
      541 GGGTGGAGTA TTTACGGTAA ACTGCCCACT TGGCAGTACA TCAAGTGTAT CATATGCCAA 
      601 GTCCGCCCCC TATTGACGTC AATGACGGTA AATGGCCCGC CTGGCATTAT GCCCAGTACA 
      661 TGACCTTACG GGACTTTCCT ACTTGGCAGT ACATCTACGT ATTAGTCATC GCTATTACCA 
      721 TGGTGATGCG GTTTTGGCAG TACACCAATG GGCGTGGATA GCGGTTTGAC TCACGGGGAT 
      781 TTCCAAGTCT CCACCCCATT GACGTCAATG GGAGTTTGTT TTGGCACCAA AATCAACGGG 
      841 ACTTTCCAAA ATGTCGTAAT AACCCCGCCC CGTTGACGCA AATGGGCGGT AGGCGTGTAC 
      901 GGTGGGAGGT CTATATAAGC AGAGCTCGTT TAGTGAACCG TCAGAATTTT GTAATACGAC 
      961 TCACTATAGG GCGGCCGGGA ATTCGTCGAC TGGATCCGGT ACCGAGGAGA TCTGCCGCCG 
     1021 CGATCGCCAT GGGGACCACA GCCCCAGGGC CTGCTAACCT TCTGGACCTA TGTGACCAGA 
     1081 AACTCCTGGA CTTTGTCTAC AATGTGGACA ACAAGGACTT CATGTGGCTT AAGGAGATTG 
     1141 AGGAGGAGGC CGAGCGAATG TTTGTCAGAG AATTCAACAA TGAGCCAGAG CTGATGCCCA 
     1201 AAACTCCTTC TCAGAAAAAC CGTCGGAAGA AGAGGCGGGT GTCTAACATT CAGGATGAAA 
     1261 ACAGAGATCC TGTGAGGAAA AGGTTGTCCC GCAGAAAGTC TCGGAGCAGT CAGGTAGGCC 
     1321 CCCGGCACCG CCGCAGCAAG CTGGGTGCTG TTGTTGAGGA GAATGGTTCG CCTGTGTTGC 
     1381 GTCGGATAAC CCGGGCAGCA GCTGCAGCAG CAGCAGCGGC AGCATCAACG TCAGCGTCTG 
     1441 TGGCATCAGC TTCTTCTTCT CCCACTACGG TGTCTCCCAT AGTGCTAATC AAAAAAGCTG 
     1501 TGGTAGAGGT AAGCACCGGT GAGCAACTGA GTGCAGAGCT TCAACTCATT AAGCTCAAAG 
     1561 GTGGCCTTCC TCAGTCTTCA GTCCCAGCTC CAGCCACATC TTCAGTTTCC CAAGGCACCC 
     1621 TGACCTCAGA AGAGGAAGCA ACACCCAAGA AATCAGAGGC TGGGAGGCTG GACTCTGCCA 
     1681 CAGACTCCGT TGCAGTGAAC TCCCTCAAGG CTACCCCTCA GAGCCCCAAG AACCGAGGGG 
     1741 TTGGGGAAGC ACGTTCTGTT TCCAAGCTCA AGATTGCTCG GGCCTCCAGG GGCCTGCAGG 
     1801 ACTCTCCTGG CTCTACGGAC TCTCCATGGC AAGAGCGGGT GCTTTCTCCT ATTCTGCCGA 
     1861 ATAACATCTT GTCCACCACA GCCAAGAACC CTCTTGGGAG TATTCAGTCA GTGCAGCAAA 
     1921 GCCTGATTTC CCAGGATTCC CAAGTGCCAT TAGCCAAAAA GTATTGTTTG GTGACTCAAC 
     1981 AAGAAAATGC CAGTCGCAGA TCAAGCAGGA TTGCTAAGAA GGCTGGCAAA GAGCCAGAGG 
     2041 CCTCTGCTCG CATCATTTGT CACAGTTACC TGGAGAGGCT CCTGAATGTT GAAGTGCCTC 
     2101 AGAATGTTGG CCTGGAAAAG GAGCCTGTTG AGGTGGCCGA GCCTGAGGAG GCAGTAGAGG 
     2161 AGCAAGAGGT CTCCAAGAAC AGTGGATGTT CCTCCAAACC CCACAGTGCC ACCAAGATGG 
     2221 CGATCAGTAC GCCTACCTCG AAGCCTGTGG CCACTGGCCA GGCAACAGCT GTTGAAGAGC 
     2281 AAGACGCTGA GCTTGACCAA ACAGATGGAC ACAGAGAACC ACCCCAGAGT GTCAGGAGGA 
     2341 AACGCAGTTA CAAGCAGGCC ATGAGTGAAC TGGATGAGGA GCATCTGGAG GATGAAGAGC 
     2401 TGCAGCCTCC CCGGAACAAG ACCCCTTCCC CACCCTGTCC AGCCAGCAAG GTGGTACGGC 
     2461 CCCTCCGGAC TTTCTTGCAC ACAGTGCAGA AGAACCAGAT GCTCATGACC CCTACTCTGG 
     2521 CCTCCCGCAG CAGTGTCATG AAGTCCTTTA TTAAACGCAA CACTCCACTC CGTGTGGACC 
     2581 CTAAGGAGAA GGAGCGCCAG CGCCTAGAGA GCCTGCGGCG GAAGGAGGAG GCCGAGCAGC 
     2641 TGCGCAGACA GAAGGTAGAG GAAGACAAGC GGCGGCGCCT GGAGGAGGTG AAGCTGAAGC 
     2701 GTGAAGAGCG CCTCCGCAAG GTATTGCAGG CTCGTGAGCG GGTGGAACAG ATGAAGGAGG 
     2761 AAAAGAAGAA GCAGATTGAG CAGAAGTTTG CTCAGATTGA TGAGAAGACA GAGAAGGCTA 
     2821 AGGAGGAGCG GCTGGCAGAG AAGGCCAAGA AGAAGGCGGC CGCCAAGAAG ATGGAAGAGA 
     2881 TGGAGGCGCG CAGGAAGCAG GAGGAGGAGG CGCGCAGGCT CAAGTGGCTC CAGCAGGAGG 
     2941 AAGAGGAGCG GCGGCATCAA GAAATGCTAC AGCGGAAGAA GGAGGAAGAA CAGGAGCGCC 
     3001 GCAAAGCAGC TGAGGCCAGG CGACTGGCAG AACAGCGTGA GCAAGAGCGG CGGAGAGAGC 
     3061 AAGAACGGAG AGAGCAGGAA CGGAGAGAGC AGGAGCGGAG AGAGCAGGAG CGGAGAGAGC 
     3121 AGGAGCGGAG GGAGCAGGAG CGGAGAGAGC AGGAACGGAG GGAGCAGGAA CGACTTCGGG 
     3181 CCAAGAGGGA GATGCAGGAG AGAGAGAAGG CCCTGCGGCT CCAAAAGGAA CGACTTCAGA 
     3241 GGGAACTGGA AGAGAAGAAG AAGAAGGAAG AGCAGCAGCG CCTGGCTGAG CAGCAACTGC 
     3301 AGGAGGAGCA GGCAAAGAAA GCTAAGGAGG TGGCCGCCGG CAAAGCACTG AACATGACTG 
     3361 TGGATGTGCA GTCTCCTGTT TGTACCTCAT ATCAGATGAC TCCACAAGGA CCCAAGTCCT 
     3421 TTCCCAAGAT CAGCATGGAT GACTATGGGA TGGACCTGAA TAGCGATGAC TCCACAGATG 
     3481 ATGAGGCCCA TCCCCGGAAA CCCATCCCTT CCTGGGCCAA AGGCACTCAG CTCAGCCAGG 
     3541 CCATCGTCCA CCAGTACTAC CACCCCCCAA ACATTCTAGA GCTCTTCGGA TCTATTCTCC 
     3601 CACTGGACTT GGAGGACATC TTTAAGAAGA GGAAGACTCG CTACCACAAG CGCACCAGCT 
     3661 CTGCTGTTTG GAACTCACCA CCTCTGAAAG CCACCATCCC CAGCAGTGGG GACACGCGTA 
     3721 CGCGGCCGCT CGAGCAGAAA CTCATCTCAG AAGAGGATCT GGCAGCAAAT GATATCCTGG 
     3781 ATTACAAGGA TGACGACGAT AAGGTTTAAA CGGCCGGCCG CGGTCATAGC TGTTTCCTGA 
     3841 ACAGATCCCG GGTGGCATCC CTGTGACCCC TCCCCAGTGC CTCTCCTGGC CCTGGAAGTT 
     3901 GCCACTCCAG TGCCCACCAG CCTTGTCCTA ATAAAATTAA GTTGCATCAT TTTGTCTGAC 
     3961 TAGGTGTCCT TCTATAATAT TATGGGGTGG AGGGGGGTGG TATGGAGCAA GGGGCAAGTT 
     4021 GGGAAGACAA CCTGTAGGGC CTGCGGGGTC TATTGGGAAC CAAGCTGGAG TGCAGTGGCA 
     4081 CAATCTTGGC TCACTGCAAT CTCCGCCTCC TGGGTTCAAG CGATTCTCCT GCCTCAGCCT 
     4141 CCCGAGTTGT TGGGATTCCA GGCATGCATG ACCAGGCTCA GCTAATTTTT GTTTTTTTGG 
     4201 TAGAGACGGG GTTTCACCAT ATTGGCCAGG CTGGTCTCCA ACTCCTAATC TCAGGTGATC 
     4261 TACCCACCTT GGCCTCCCAA ATTGCTGGGA TTACAGGCGT GAACCACTGC TCCCTTCCCT 
     4321 GTCCTTCTGA TTTTAAAATA ACTATACCAG CAGGAGGACG TCCAGACACA GCATAGGCTA 
     4381 CCTGGCCATG CCCAACCGGT GGGACATTTG AGTTGCTTGC TTGGCACTGT CCTCTCATGC 
     4441 GTTGGGTCCA CTCAGTAGAT GCCTGTTGAA TTGGGTACGC GGCCAGCGGC GAGCGGTATC 
     4501 AGCTCACTCA AAGGCGGTAA TACGGTTATC CACAGAATCA GGGGATAACG CAGGAAAGAA 
     4561 CATGTGAGCA AAAGGCCAGC AAAAGGCCAG GAACCGTAAA AAGGCCGCGT TGCTGGCGTT 
     4621 TTTCCATAGG CTCCGCCCCC CTGACGAGCA TCACAAAAAT CGACGCTCAA GTCAGAGGTG 
     4681 GCGAAACCCG ACAGGACTAT AAAGATACCA GGCGTTTCCC CCTGGAAGCT CCCTCGTGCG 
     4741 CTCTCCTGTT CCGACCCTGC CGCTTACCGG ATACCTGTCC GCCTTTCTCC CTTCGGGAAG 
     4801 CGTGGCGCTT TCTCATAGCT CACGCTGTAG GTATCTCAGT TCGGTGTAGG TCGTTCGCTC 
     4861 CAAGCTGGGC TGTGTGCACG AACCCCCCGT TCAGCCCGAC CGCTGCGCCT TATCCGGTAA 
     4921 CTATCGTCTT GAGTCCAACC CGGTAAGACA CGACTTATCG CCACTGGCAG CAGCCACTGG 
     4981 TAACAGGATT AGCAGAGCGA GGTATGTAGG CGGTGCTACA GAGTTCTTGA AGTGGTGGCC 
     5041 TAACTACGGC TACACTAGAA GAACAGTATT TGGTATCTGC GCTCTGCTGA AGCCAGTTAC 
     5101 CTTCGGAAAA AGAGTTGGTA GCTCTTGATC CGGCAAACAA ACCACCGCTG GTAGCGGTGG 
     5161 TTTTTTTGTT TGCAAGCAGC AGATTACGCG CAGAAAAAAA GGATCTCAAG AAGATCCTTT 
     5221 GATCTTTTCT ACGGGGTCTG ACGCTCAGTG GAACGAAAAC TCACGTTAAG GGATTTTGGT 
     5281 CATGAGATTA TCAAAAAGGA TCTTCACCTA GATCCTTTTA AATTAAAAAT GAAGTTTTAA 
     5341 ATCAATCTAA AGTATATATG AGTAACCTGA GGCTATGGCA GGGCCTGCCG CCCCGACGTT 
     5401 GGCTGCGAGC CCTGGGCCTT CACCCGAACT TGGGGGGTGG GGTGGGGAAA AGGAAGAAAC 
     5461 GCGGGCGTAT TGGCCCCAAT GGGGTCTCGG TGGGGTATCG ACAGAGTGCC AGCCCTGGGA 
     5521 CCGAACCCCG CGTTTATGAA CAAACGACCC AACACCGTGC GTTTTATTCT GTCTTTTTAT 
     5581 TGCCGTCATA GCGCGGGTTC CTTCCGGTAT TGTCTCCTTC CGTGTTTCAG TTAGCCTCCC 
     5641 CCTAGGGTGG GCGAAGAACT CCAGCATGAG ATCCCCGCGC TGGAGGATCA TCCAGCCGGC 
     5701 GTCCCGGAAA ACGATTCCGA AGCCCAACCT TTCATAGAAG GCGGCGGTGG AATCGAAATC 
     5761 TCGTGATGGC AGGTTGGGCG TCGCTTGGTC GGTCATTTCG AACCCCAGAG TCCCGCTCAG 
     5821 AAGAACTCGT CAAGAAGGCG ATAGAAGGCG ATGCGCTGCG AATCGGGAGC GGCGATACCG 
     5881 TAAAGCACGA GGAAGCGGTC AGCCCATTCG CCGCCAAGCT CTTCAGCAAT ATCACGGGTA 
     5941 GCCAACGCTA TGTCCTGATA GCGATCCGCC ACACCCAGCC GGCCACAGTC GATGAATCCA 
     6001 GAAAAGCGGC CATTTTCCAC CATGATATTC GGCAAGCAGG CATCGCCATG GGTCACGACG 
     6061 AGATCCTCGC CGTCGGGCAT GCTCGCCTTG AGCCTGGCGA ACAGTTCGGC TGGCGCGAGC 
     6121 CCCTGATGCT CTTCGTCCAG ATCATCCTGA TCGACAAGAC CGGCTTCCAT CCGAGTACGT 
     6181 GCTCGCTCGA TGCGATGTTT CGCTTGGTGG TCGAATGGGC AGGTAGCCGG ATCAAGCGTA 
     6241 TGCAGCCGCC GCATTGCATC AGCCATGATG GATACTTTCT CGGCAGGAGC AAGGTGAGAT 
     6301 GACAGGAGAT CCTGCCCCGG CACTTCGCCC AATAGCAGCC AGTCCCTTCC CGCTTCAGTG 
     6361 ACAACGTCGA GCACAGCTGC GCAAGGAACG CCCGTCGTGG CCAGCCACGA TAGCCGCGCT 
     6421 GCCTCGTCTT GCAGTTCATT CAGGGCACCG GACAGGTCGG TCTTGACAAA AAGAACCGGG 
     6481 CGCCCCTGCG CTGACAGCCG GAACACGGCG GCATCAGAGC AGCCGATTGT CTGTTGTGCC 
     6541 CAGTCATAGC CGAATAGCCT CTCCACCCAA GCGGCCGGAG AACCTGCGTG CAATCCATCT 
     6601 TGTTCAATCA TGCGAAACGA TCCTCATCCT GTCTCTTGAT CGATCTTTGC AAAAGCCTAG 
     6661 GCCTCCAAAA AAGCCTCCTC ACTACTTCTG GAATAGCTCA GAGGCCGAGG CGGCCTCGGC 
     6721 CTCTGCATAA ATAAAAAAAA TTAGTCAGCC ATGGGGCGGA GAATGGGCGG AACTGGGCGG 
     6781 AGTTAGGGGC GGGATGGGCG GAGTTAGGGG CGGGACTATG GTTGCTGACT AATTGAGATG 
     6841 CATGCTTTGC ATACTTCTGC CTGCTGGGGA GCCTGGGGAC TTTCCACACC TGGTTGCTGA 
     6901 CTAATTGAGA TGCATGCTTT GCATACTTCT GCCTGCTGGG GAGCCTGGGG ACTTTCCACA 
     6961 CCCTAACTGA CACACATTCC ACAGCTGGTT CTTTCCGCCT CAGGACTCTT CCTTTTTCAA 
     7021 TATTATTGAA GCATTTATCA GGGTTATTGT CTCATGAGCG GATACATATT TGAATGTATT 
     7081 TAGAAAAATA AACAAATAGG GGTTCCGCGC ACATTTCCCC GAAAAGTGCC ACCTGACGCG 
     7141 CCCTGTAGCG GCGCATTAAG CGCGGCGGGT GTGGTGGTTA CGCGCAGCGT GACCGCTACA 
     7201 CTTGCCAGCG CCCTAGCGCC CGCTCCTTTC GCTTTCTTCC CTTCCTTTCT CGCCACGTTC 
     7261 GCCGGCTTTC CCCGTCAAGC TCTAAATCGG GGGCTCCCTT TAGGGTTCCG ATTTAGTGCT 
     7321 TTACGGCACC TCGACCCCAA AAAACTTGAT TAGGGTGATG GTTCACGTAG TGGGCCATCG 
     7381 CCCTGATAGA CGGTTTTTCG CCCTTTGACG TTGGAGTCCA CGTTCTTTAA TAGTGGACTC 
     7441 TTGTTCCAAA CTGGAACAAC ACTCAACCCT ATCTCGGTCT ATTCTTTTGA TTTATAAGGG 
     7501 ATTTTGCCGA TTTCGGCCTA TTGGTTAAAA AATGAGCTGA TTTAACAAAA ATTTAACGCG 
     7561 AATTTT
//